STEFAN KAPPESummaryAffiliation: Seattle Biomedical Research Institute Country: USA Publications
Research Grants
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Detail Information
Publications
Host cell transcriptional profiling during malaria liver stage infection reveals a coordinated and sequential set of biological eventsSónia S Albuquerque
Unidade de Malaria, Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Lisboa, Portugal
BMC Genomics 10:270. 2009..Although this constitutes an essential step of malaria infection, the requirements of Plasmodium parasites in liver cells and how they use the host cell for their own survival and development are poorly understood...- Malaria parasite development in the mosquito and infection of the mammalian hostAhmed S I Aly
Seattle Biomedical Research Institute, Seattle, Washington 98109, USA
Annu Rev Microbiol 63:195-221. 2009..Here, we discuss what is known about the molecular and cellular basis of the developmental progression of parasites and their interactions with host tissues in the mosquito and during the early phase of mammalian infection... 
Plasmodium sporozoite molecular cell biologyStefan H I Kappe
Seattle Biomedical Research Institute, Seattle, WA 98109 1651, USA
Annu Rev Cell Dev Biol 20:29-59. 2004....- Apicomplexan gliding motility and host cell invasion: overhauling the motor modelStefan H I Kappe
Seattle Biomedical Research Institute, 4 Nickerson Street, Suite 200, Seattle, WA 98109-1651, USA
Trends Parasitol 20:13-6. 2004 - Plasmodium yoelii sporozoites with simultaneous deletion of P52 and P36 are completely attenuated and confer sterile immunity against infectionMehdi Labaied
Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109 5219, USA
Infect Immun 75:3758-68. 2007..The study will critically guide the design of Plasmodium falciparum live attenuated malaria vaccines... - Distinct malaria parasite sporozoites reveal transcriptional changes that cause differential tissue infection competence in the mosquito vector and mammalian hostSebastian A Mikolajczak
Seattle Biomedical Research Institute, Seattle, WA 98109 5219, USA
Mol Cell Biol 28:6196-207. 2008..Genes identified herein might represent targets for vector-based transmission blocking strategies (UOS genes), as well as strategies that prevent mammalian host infection (UIS genes)... - Targeted deletion of SAP1 abolishes the expression of infectivity factors necessary for successful malaria parasite liver infectionAhmed S I Aly
Seattle Biomedical Research Institute, Seattle, WA 98109, USA
Mol Microbiol 69:152-63. 2008..These findings demonstrate that SAP1 is essential for liver infection possibly by functioning as a selective regulator controlling the expression of infectivity-associated parasite effector genes... - Quantitative isolation and in vivo imaging of malaria parasite liver stagesAlice S Tarun
Seattle Biomedical Research Institute, Seattle, WA 98109, USA
Int J Parasitol 36:1283-93. 2006..Intravital imaging thus reveals new, important information on the malaria parasite's transition from tissue to blood stage... 
An efficient strategy for gene targeting and phenotypic assessment in the Plasmodium yoelii rodent malaria modelSebastian A Mikolajczak
Seattle Biomedical Research Institute, Seattle, WA 98109, USA
Mol Biochem Parasitol 158:213-6. 2008..This allows for a more rapid assessment of parasite growth in all of its developmental stages. In addition, the introduction of the fluorescent marker via the replacement strategy confers the stable integration of the marker...- Depletion of the Plasmodium berghei thrombospondin-related sporozoite protein reveals a role in host cell entry by sporozoitesMehdi Labaied
Seattle Biomedical Research Institute, Seattle, WA 98109, USA
Mol Biochem Parasitol 153:158-66. 2007..Thus, TRSP is an additional TSR-containing malaria parasite protein that is mainly involved in initial infection of the mammalian host... - L-FABP is a critical host factor for successful malaria liver stage developmentSebastian A Mikolajczak
Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109 5219, USA
Int J Parasitol 37:483-9. 2007..This is the first identified direct liver stage-host cell protein interaction, providing a possible explanation for the importance of UIS3 in liver infection... - Protracted sterile protection with Plasmodium yoelii pre-erythrocytic genetically attenuated parasite malaria vaccines is independent of significant liver-stage persistence and is mediated by CD8+ T cellsAlice S Tarun
Seattle Biomedical Research Institute, Seattle, WA 98109, USA
J Infect Dis 196:608-16. 2007..These findings have important implications for the development of a P. falciparum GAP malaria vaccine... - Type II fatty acid synthesis is essential only for malaria parasite late liver stage developmentAshley M Vaughan
Seattle Biomedical Research Institute, Seattle, WA 98109, USA
Cell Microbiol 11:506-20. 2009..Malaria parasites therefore depend on the intrinsic FAS II pathway only at one specific life cycle transition point, from liver to blood... - Malaria parasite pre-erythrocytic stage infection: gliding and hidingAshley M Vaughan
Seattle Biomedical Research Institute, Seattle, WA 98109, USA
Cell Host Microbe 4:209-18. 2008..We discuss the factors, both parasite and host, involved in the interactions that occur during this "silent" phase of infection... - Preerythrocytic, live-attenuated Plasmodium falciparum vaccine candidates by designKelley M VanBuskirk
Seattle Biomedical Research Institute, Seattle, WA 98109, USA
Proc Natl Acad Sci U S A 106:13004-9. 2009..GAPs might provide a safe and reproducible platform to develop an efficacious whole-cell malaria vaccine that prevents infection at the preerythrocytic stage... - Subpatent infection with nucleoside transporter 1-deficient Plasmodium blood stage parasites confers sterile protection against lethal malaria in miceAhmed S I Aly
Seattle Biomedical Research Institute, Seattle, WA 98109, USA
Cell Microbiol 12:930-8. 2010..The study demonstrates that genetic manipulation provides a platform for the designed, complete attenuation of malaria parasite blood stages and suggests testing the safety and efficacy of P. falciparum NT1 knockout strains in humans... - Redefining the role of de novo fatty acid synthesis in Plasmodium parasitesAlice S Tarun
Seattle Biomedical Research Institute, Seattle, WA 98109, USA Infectious Diseases and Microbiology, University of Pittsburgh PA 15261, USA
Trends Parasitol 25:545-50. 2009..We discuss the role of fatty acid metabolism in Plasmodium and why we believe that de novo fatty acid synthesis is only required for parasite late liver-stage development... - A combined transcriptome and proteome survey of malaria parasite liver stagesAlice S Tarun
Seattle Biomedical Research Institute, Seattle, WA 98109, USA
Proc Natl Acad Sci U S A 105:305-10. 2008.... - That was then but this is now: malaria research in the time of an eradication agendaStefan H I Kappe
Seattle Biomedical Research Institute, Seattle, WA 98109, USA
Science 328:862-6. 2010..Here, we highlight some of the research into malaria parasite biology that has the potential to provide new intervention targets for antimalarial drugs and vaccines... - Preerythrocytic malaria vaccine developmentSebastian A Mikolajczak
Seattle Biomedical Research Institute, Seattle, Washington 98109 5219, USA
Curr Opin Infect Dis 20:461-6. 2007..This review examines the potential of current preerythrocytic stage malaria vaccine approaches to reduce the global burden of malaria... - Plasmodium pyruvate dehydrogenase activity is only essential for the parasite's progression from liver infection to blood infectionYing Pei
Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109, USA
Mol Microbiol 75:957-71. 2010..This phenotype is similar to that observed for deletions of genes involved in FAS II elongation. The data strongly support the hypothesis that the sole role of PDH is to provide acetyl-CoA for FAS II... - Advances and challenges in malaria vaccine developmentRuobing Wang
Seattle Biomedical Research Institute and Department of Global Health, University of Washington, Seattle, Washington, USA
Expert Rev Mol Med 11:e39. 2009..Elimination of malaria will probably ultimately depend on the development of highly effective vaccines... - A dispensable Plasmodium locus for stable transgene expressionVanessa Y Jacobs-Lorena
Seattle Biomedical Research Institute, Seattle, WA 98109 5219, USA
Mol Biochem Parasitol 171:40-4. 2010..yoelii wildtype parasites. Further, we show that a fluorescent transgene can be stably expressed from this site. This demonstrates that the S1 locus can be utilized for stable expression of heterologous genes in rodent malaria parasites... - Plasmodium falciparum PF10_0164 (ETRAMP10.3) is an essential parasitophorous vacuole and exported protein in blood stagesDrew C MacKellar
Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109 5219, USA
Eukaryot Cell 9:784-94. 2010..We conclude that PF10_0164 is a parasitophorous vacuole protein that is essential in asexual blood stages and that does not complement P. yoelii UIS4, and it is thus likely not a functional ortholog of UIS4... - Genetically engineered, attenuated whole-cell vaccine approaches for malariaAshley M Vaughan
Seattle Biomedical Research Institute, Seattle, WA, USA
Hum Vaccin 6:107-13. 2010.... - Assessment and improvement of the Plasmodium yoelii yoelii genome annotation through comparative analysisAshley Vaughan
Seattle Biomedical Research Institute, Seattle, WA 98109, USA
Bioinformatics 24:i383-9. 2008..Unfortunately, only preliminary gene models were annotated on the partially sequenced genome, mostly by in silico gene prediction, and there has been no major improvement of the annotation since 2002... - A member of a conserved Plasmodium protein family with membrane-attack complex/perforin (MACPF)-like domains localizes to the micronemes of sporozoitesKarine Kaiser
Department of Pathology, Michael Heidelberger Division, New York University School of Medicine, New York, NY 10016, USA
Mol Biochem Parasitol 133:15-26. 2004..MACPF-like proteins may play important roles in parasite interactions with the mosquito vector and transmission to the vertebrate host... - Infectivity-associated changes in the transcriptional repertoire of the malaria parasite sporozoite stageKai Matuschewski
Michael Heidelberger Division, Department of Pathology, New York University School of Medicine, New York 10016, USA
J Biol Chem 277:41948-53. 2002..Developmental up-regulation of specific mRNAs in the infectious transmission stage of Plasmodium indicates that their translation products may have unique roles in hepatocyte infection and/or development of liver stages... - Myosin A tail domain interacting protein (MTIP) localizes to the inner membrane complex of Plasmodium sporozoitesLawrence W Bergman
Division of Molecular Parasitology, Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA 19129, USA
J Cell Sci 116:39-49. 2003..The actin/TRAP complex is then redistributed by the stationary MyoA from the anterior to the posterior end of the zoite, leading to its forward movement on a substrate or to penetration of a host cell... - The Plasmodium sporozoite journey: a rite of passageStefan H I Kappe
Michael Heidelberger Division, Department of Pathology, New York University School of Medicine, New York, NY 10016, USA
Trends Parasitol 19:135-43. 2003..This article discusses recent work, mostly done with rodent malaria parasites, that has contributed to a better understanding of the sporozoites' complex biology and which has opened up new avenues for future sporozoite research... 
Transformation of sporozoites into early exoerythrocytic malaria parasites does not require host cellsKarine Kaiser
Michael Heidelberger Division, Dept. of Pathology, New York University School of Medicine, New York, NY 10016, USA
J Exp Med 197:1045-50. 2003....- Differential transcriptome profiling identifies Plasmodium genes encoding pre-erythrocytic stage-specific proteinsKarine Kaiser
Michael Heidelberger Division, Department of Pathology, New York University School of Medicine, New York, NY 10016, USA
Mol Microbiol 51:1221-32. 2004.... - Genetically modified Plasmodium parasites as a protective experimental malaria vaccineAnn-Kristin Mueller
Department of Parasitology, Heidelberg University School of Medicine, Heidelberg 69120, Germany
Nature 433:164-7. 2005..This protection is sustained and stage specific. Our findings demonstrate that a safe and effective, genetically attenuated whole-organism malaria vaccine is possible... - Microbiology. Malaria's stealth shuttleAlan F Cowman
Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia
Science 313:1245-6. 2006 - Genetically attenuated Plasmodium berghei liver stages induce sterile protracted protection that is mediated by major histocompatibility complex Class I-dependent interferon-gamma-producing CD8+ T cellsOusman Jobe
Division of Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA
J Infect Dis 196:599-607. 2007..On the basis of these observations, we propose that the development of genetically attenuated P. falciparum parasites is warranted for tests in clinical trials as a pre-erythrocytic stage vaccine candidate... - Heme oxygenase-1 is an anti-inflammatory host factor that promotes murine plasmodium liver infectionSabrina Epiphanio
Unidade de Malaria, Instituto de Medicina Molecular, Universidade de Lisboa, 1649 028 Lisboa, Portugal
Cell Host Microbe 3:331-8. 2008.... - Plasmodium liver stage developmental arrest by depletion of a protein at the parasite-host interfaceAnn-Kristin Mueller
Department of Parasitology, Heidelberg University School of Medicine, 69120 Heidelberg, Germany
Proc Natl Acad Sci U S A 102:3022-7. 2005..Genetically attenuated liver stages may thus induce immune responses, which inhibit subsequent infection of the liver with WT parasites... - Malaria liver stage culture: in vitro veritas?Stefan H I Kappe
Am J Trop Med Hyg 74:706-7. 2006 - Plasmodium falciparum MAEBL is a unique member of the ebl familyPeter L Blair
Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556-0369, USA
Mol Biochem Parasitol 122:35-44. 2002..These data indicate that the primary structure of maebl is highly conserved among Plasmodium species, while its characteristics demonstrate a function unique among the ebl proteins... - Malaria: progress, perils, and prospects for eradicationBrian M Greenwood
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
J Clin Invest 118:1266-76. 2008..Insights into parasite biology, human immunity, and vector behavior will guide efforts to translate parasite and mosquito genome sequences into novel interventions... - Release of hepatic Plasmodium yoelii merozoites into the pulmonary microvasculatureKerstin Baer
Department of Medical Parasitology, New York University School of Medicine, New York, New York, United States of America
PLoS Pathog 3:e171. 2007..We believe this previously unknown part of the plasmodial life cycle ensures an effective transition from the liver to the blood phase of the malaria infection...
Research Grants
- New infection-related proteins of Plasmodium sporozoites and liver stagesStefan H I Kappe; Fiscal Year: 2010..Identifying major liver stage-host hepatocyte interactions and elucidating their functional significance will reveal fundamental principles of malaria parasite liver infection and provide new avenues for preventive drug design. ..
- New invasion-related proteins of Plasmodium sporozoitesSTEFAN KAPPE; Fiscal Year: 2007..abstract_text> ..
- New infection-related proteins of Plasmodium sporozoites and liver stagesStefan H I Kappe; Fiscal Year: 2011..Identifying major liver stage-host hepatocyte interactions and elucidating their functional significance will reveal fundamental principles of malaria parasite liver infection and provide new avenues for preventive drug design. ..