M Grace

Summary

Affiliation: Schering-Plough Research Institute
Country: USA

Publications

  1. ncbi Structural and biologic characterization of pegylated recombinant IFN-alpha2b
    M Grace
    Biotechnology Development, Schering Plough Research Institute, Union, NJ 07083, USA
    J Interferon Cytokine Res 21:1103-15. 2001
  2. pmc The use of laser scanning cytometry to assess depth of penetration of adenovirus p53 gene therapy in human xenograft biopsies
    M J Grace
    Department of Biotechnology, Schering Plough Research Institute, Union, New Jersey 07083, USA
    Am J Pathol 155:1869-78. 1999
  3. ncbi Pegylated IFNs for chronic hepatitis C: an update
    Michael J Grace
    Schering Plough Research Institute, Biotechnology Development, Union, NJ 07083, USA
    Expert Opin Drug Deliv 2:219-26. 2005
  4. ncbi Pegylating IFNs at his-34 improves the in vitro antiviral activity through the JAK/STAT pathway
    Michael J Grace
    Schering Plough Research Institute, Biotechnology Development, Union, NJ, USA
    Antivir Chem Chemother 15:287-97. 2004
  5. ncbi Site of pegylation and polyethylene glycol molecule size attenuate interferon-alpha antiviral and antiproliferative activities through the JAK/STAT signaling pathway
    Michael J Grace
    Schering Plough Research Institute, Biotechnology Development, Bioanalytical and Process Development, Union, New Jersey 07083 and Aventis Pharmaceuticals, Medical Affairs Oncology, Bridgewater, New Jersey 08807, USA
    J Biol Chem 280:6327-36. 2005
  6. ncbi Biological characterization of pegylated interferons: a case study
    Lei Xie
    Schering Plough Research Institute, Union, NJ, USA
    Methods Mol Biol 308:319-35. 2005
  7. ncbi Biologic activity of polyethylene glycol12000-interferon-alpha2b compared with interferon-alpha2b: gene modulatory and antigrowth effects in tumor cells
    Keyur Vyas
    Cleveland Clinic Taussig Cancer Center and Lerner Research Institute, Ohio, USA
    J Immunother 26:202-11. 2003
  8. ncbi The use of field emission scanning electron microscopy to assess recombinant adenovirus stability
    Linda J Obenauer-Kutner
    Biotechnology Development, Schering Plough Research Institute, Union, NJ 07083, USA
    Hum Gene Ther 13:1687-96. 2002
  9. ncbi Measurement of the functional affinity constant of a monoclonal antibody for cell surface receptors using kinetic exclusion fluorescence immunoassay
    Lei Xie
    BiotechDev Bioanalytical, Schering Plough Research Institute, 1011 Morris Ave, U 13 2050, Union, NJ 07083, United States
    J Immunol Methods 304:1-14. 2005
  10. ncbi Comparative thermal stabilities of recombinant adenoviruses and hexon protein
    Peter M Ihnat
    Biotechnology Development, Schering Plough Research Institute, Union, NJ 07081 3121, USA
    Biochim Biophys Acta 1726:138-51. 2005

Collaborators

Detail Information

Publications15

  1. ncbi Structural and biologic characterization of pegylated recombinant IFN-alpha2b
    M Grace
    Biotechnology Development, Schering Plough Research Institute, Union, NJ 07083, USA
    J Interferon Cytokine Res 21:1103-15. 2001
    ..These results demonstrate that PEG Intron maintains an in vitro biologic potency profile for both antiviral and immunotherapeutic activity that is highly comparable to that of Intron A...
  2. pmc The use of laser scanning cytometry to assess depth of penetration of adenovirus p53 gene therapy in human xenograft biopsies
    M J Grace
    Department of Biotechnology, Schering Plough Research Institute, Union, New Jersey 07083, USA
    Am J Pathol 155:1869-78. 1999
    ..However, the combination therapy did cause an elevation in the number of tumor cells undergoing apoptosis...
  3. ncbi Pegylated IFNs for chronic hepatitis C: an update
    Michael J Grace
    Schering Plough Research Institute, Biotechnology Development, Union, NJ 07083, USA
    Expert Opin Drug Deliv 2:219-26. 2005
    ..Comparative trials have been initiated that will provide insight into relative importance of pharmacokinetics, bioactivity and dosing regimen...
  4. ncbi Pegylating IFNs at his-34 improves the in vitro antiviral activity through the JAK/STAT pathway
    Michael J Grace
    Schering Plough Research Institute, Biotechnology Development, Union, NJ, USA
    Antivir Chem Chemother 15:287-97. 2004
    ..The elucidation of the in vitro effects of pegylation is important and this will ultimately have a positive impact on the in vivo efficacy of treatment for patients with hepatitis C...
  5. ncbi Site of pegylation and polyethylene glycol molecule size attenuate interferon-alpha antiviral and antiproliferative activities through the JAK/STAT signaling pathway
    Michael J Grace
    Schering Plough Research Institute, Biotechnology Development, Bioanalytical and Process Development, Union, New Jersey 07083 and Aventis Pharmaceuticals, Medical Affairs Oncology, Bridgewater, New Jersey 08807, USA
    J Biol Chem 280:6327-36. 2005
    ..The higher specific activity associated with the His(34) positional isomer suggests that this site may be favorable for pegylating IFN-alpha2b molecules...
  6. ncbi Biological characterization of pegylated interferons: a case study
    Lei Xie
    Schering Plough Research Institute, Union, NJ, USA
    Methods Mol Biol 308:319-35. 2005
  7. ncbi Biologic activity of polyethylene glycol12000-interferon-alpha2b compared with interferon-alpha2b: gene modulatory and antigrowth effects in tumor cells
    Keyur Vyas
    Cleveland Clinic Taussig Cancer Center and Lerner Research Institute, Ohio, USA
    J Immunother 26:202-11. 2003
    ..Therefore, although the specific activity of IFN-alpha2b is approximately three times greater than PEG-IFN-alpha2b, the two preparations have identical in vitro biologic activities when applied to cells at equivalent antiviral units...
  8. ncbi The use of field emission scanning electron microscopy to assess recombinant adenovirus stability
    Linda J Obenauer-Kutner
    Biotechnology Development, Schering Plough Research Institute, Union, NJ 07083, USA
    Hum Gene Ther 13:1687-96. 2002
    ..The results of this study demonstrate that FESEM, coupled with sophisticated image analysis, may be an important tool in quantifying the distribution of aggregated species and assessing the overall stability of rAd samples...
  9. ncbi Measurement of the functional affinity constant of a monoclonal antibody for cell surface receptors using kinetic exclusion fluorescence immunoassay
    Lei Xie
    BiotechDev Bioanalytical, Schering Plough Research Institute, 1011 Morris Ave, U 13 2050, Union, NJ 07083, United States
    J Immunol Methods 304:1-14. 2005
    ..Taken together, we believe these data can provide valuable insight into antibody behavior in vivo, especially in the case of insoluble or difficult to purify transmembrane receptors...
  10. ncbi Comparative thermal stabilities of recombinant adenoviruses and hexon protein
    Peter M Ihnat
    Biotechnology Development, Schering Plough Research Institute, Union, NJ 07081 3121, USA
    Biochim Biophys Acta 1726:138-51. 2005
    ..However, at lower temperatures, disappearance of single virions resulted from competing irreversible degradation and aggregation reactions...
  11. ncbi Use of an automated image processing program to quantify recombinant adenovirus particles
    Linda J Obenauer-Kutner
    Biotechnology Development, Schering Plough Research Institute, Union, NJ 07083, USA
    Microsc Microanal 11:37-41. 2005
    ..The application and utility of this macro program has enabled us to rapidly and efficiently analyze very large data sets of rAd samples while minimizing operator time...
  12. ncbi Regulation of gene expression by pegylated IFN-alpha2b and IFN-alpha2b in human peripheral blood mononuclear cells
    Diana L Brassard
    Department of Biotechnology, Schering Plough Research Institute, Union, NJ 07083, USA
    J Interferon Cytokine Res 24:455-69. 2004
    ....
  13. ncbi Successful adenovirus-mediated wild-type p53 gene transfer in patients with bladder cancer by intravesical vector instillation
    Jürgen Kuball
    Department of Medicine III, Johannes Gutenberg University, Mainz, Germany
    J Clin Oncol 20:957-65. 2002
    ..To study safety, feasibility, and biologic activity of adenovirus-mediated p53 gene transfer in patients with bladder cancer...
  14. ncbi Interferon-alpha as an immunotherapeutic protein
    Diana L Brassard
    Bioanalytical Development, Schering Plough Research Institute, Union, New Jersey 07083, USA
    J Leukoc Biol 71:565-81. 2002
    ..This review will describe the current state of knowledge of IFN-alpha as an immunoregulatory protein and address specific issues of IFN-alpha as an immunotherapeutic for antineoplastic and antiviral diseases...
  15. doi Imaging glycosylation
    Hongda Wang
    Center for Single Molecule Biophysics, The Biodesign Institute, Arizona State University, Tempe, Arizona 85287 1604, USA
    J Am Chem Soc 130:8154-5. 2008
    ....