Research Topics
| DANIEL HOFTSummaryAffiliation: Saint Louis University Country: USA Publications
Research Grants
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Detail Information
Publications
A recombinant adenovirus expressing immunodominant TB antigens can significantly enhance BCG-induced human immunityDaniel F Hoft
Division of Infectious Diseases, Allergy and Immunology, Departments of Internal Medicine and Molecular Microbiology, Saint Louis University Medical Center and Center for Vaccine Development, Saint Louis, MO 63104, USA
Vaccine 30:2098-108. 2012..Despite the availability of Bacille Calmette Guérin (BCG) vaccines, Mycobacterium tuberculosis currently infects billions of people and millions die annually from tuberculosis (TB) disease. New TB vaccines are urgently needed...
Trans-sialidase recombinant protein mixed with CpG motif-containing oligodeoxynucleotide induces protective mucosal and systemic trypanosoma cruzi immunity involving CD8+ CTL and B cell-mediated cross-primingDaniel F Hoft
Department of Internal Medicine, Saint Louis University Health Sciences Center, Saint Louis, MO 63110, USA
J Immunol 179:6889-900. 2007....
Type 1 immunity provides optimal protection against both mucosal and systemic Trypanosoma cruzi challengesD F Hoft
Department of Internal Medicine, Saint Louis University Health Sciences Center, Missouri 63110, USA
Infect Immun 70:6715-25. 2002..We conclude that future vaccines for T. cruzi and possibly other mucosally invasive, intracellular pathogens should induce both mucosal and systemic type 1 immunity...- Investigation of the relationships between immune-mediated inhibition of mycobacterial growth and other potential surrogate markers of protective Mycobacterium tuberculosis immunityDaniel F Hoft
Division of Infectious Diseases and Immunology and Vaccine and Treatment Evaluation Unit, Saint Louis University Health Sciences Center, St Louis, Missouri 63110, USA
J Infect Dis 186:1448-57. 2002..Despite previous evidence that type 1 immune responses are essential for TB immunity, interferon-gamma production did not correlate with mycobacterial inhibition. These results have important implications for TB vaccine development... - Tuberculosis vaccine development: goals, immunological design, and evaluationDaniel F Hoft
Division of Immunobiology, Department of Internal Medicine, Saint Louis University Health Sciences Center, St Louis, MO, USA
Lancet 372:164-75. 2008.... - A new recombinant bacille Calmette-Guérin vaccine safely induces significantly enhanced tuberculosis-specific immunity in human volunteersDaniel F Hoft
Department of Internal Medicine and Molecular Biology, Saint Louis University Medical Center, and Center for Vaccine Development, Saint Louis, MO 63104, USA
J Infect Dis 198:1491-501. 2008..rBCG30, which overexpresses the Mycobacterium tuberculosis secreted antigen Ag85b, was the first rBCG shown to induce significantly greater protection against TB in animals than parental BCG... - Optimizing vaccine developmentDaniel F Hoft
Division of Infectious Diseases, Allergy and Immunology, Saint Louis University School of Medicine, St Louis, MO, USA
Cell Microbiol 13:934-42. 2011..This review presents general concepts relevant for the design and development of ideal vaccines protective against diverse pathogens... - Live and inactivated influenza vaccines induce similar humoral responses, but only live vaccines induce diverse T-cell responses in young childrenDaniel F Hoft
Department of Internal Medicine, Saint Louis University Vaccine and Treatment Evaluation Unit, Missouri, USA
J Infect Dis 204:845-53. 2011..However, whether combinations of LAIV and TIV are safe and equally immunogenic is unknown. Furthermore, LAIV is more protective than TIV in children for unclear reasons... - Mucosal bacille calmette-Guérin vaccination of humans inhibits delayed-type hypersensitivity to purified protein derivative but induces mycobacteria-specific interferon-gamma responsesD F Hoft
Saint Louis University Vaccine and Treatment Evaluation Unit, Department of Internal Medicine, Saint Louis University Health Sciences Center, St Louis, MO 63110, USA
Clin Infect Dis 30:S217-22. 2000..More detailed studies of cytokine and homing molecule expression indicated that differential mucosal versus cutaneous trafficking may explain the dissociation between IFN-gamma and DTH responses... - Clinical reactogenicity of intradermal bacille Calmette-Guérin vaccinationD F Hoft
Department of Internal Medicine, Saint Louis University Health Sciences Center, Missouri, USA
Clin Infect Dis 28:785-90. 1999..These results demonstrate the clinical reactogenicity of BCG and the potential risk of contact spread of the vaccine strain and suggest that clinical reactogenicity is a trade-off for the induction of protective mycobacterial immunity... - Involvement of CD4(+) Th1 cells in systemic immunity protective against primary and secondary challenges with Trypanosoma cruziD F Hoft
Departments of Internal Medicine, Saint Louis University Health Sciences Center, St Louis, Missouri 63110, USA
Infect Immun 68:197-204. 2000..cruzi systemic immunity may be to provide helper activity for the development of other effector functions protective in vivo... - Persistence and boosting of bacille Calmette-Guérin-induced delayed-type hypersensitivityD F Hoft
Division of Infectious Diseases and Immunology, Saint Louis University Health Sciences Center, Missouri 63110, USA
Ann Intern Med 131:32-6. 1999..Bacille Calmette-Guérin (BCG) vaccination may induce persistent and booster purified protein derivative (PPD) responses that complicate tuberculosis screening efforts... - Bacille Calmette-Guérin vaccination enhances human gamma delta T cell responsiveness to mycobacteria suggestive of a memory-like phenotypeD F Hoft
St Louis University Vaccine Treatment and Evaluation Unit, Department of Internal Medicine, St Louis University Health Sciences Center, MO 63110, USA
J Immunol 161:1045-54. 1998..Our results suggest that gamma delta T cells may be capable of developing a memory immune-like phenotype, and therefore might be important targets for new vaccines... - Type 1 immunity provides both optimal mucosal and systemic protection against a mucosally invasive, intracellular pathogenDaniel F Hoft
Division of Infectious Diseases and Immunology, Saint Louis University Health Sciences Center, 3635 Vista Avenue, St Louis, MO 63110, USA
Infect Immun 73:4934-40. 2005..These results strongly confirm our earlier conclusion from studies with polarizing vaccination protocols that type 1 immunity provides optimal mucosal and systemic protection against a mucosally invasive, intracellular pathogen... - Intranasal vaccinations with the trans-sialidase antigen plus CpG Adjuvant induce mucosal immunity protective against conjunctival Trypanosoma cruzi challengesO K Giddings
Department of Molecular Microbiology and Immunology, Saint Louis University Medical Center, St Louis, Missouri 63104, USA
Infect Immun 78:1333-8. 2010..We also provide the first direct evidence that mucosal antibodies induced by intranasal TS vaccination can inhibit parasite invasion... - Differential effects of control and antigen-specific T cells on intracellular mycobacterial growthS Worku
Department of Internal Medicine, Saint Louis University Center for Vaccine Development, St. Louis, MO 63110, USA
Infect Immun 71:1763-73. 2003..These culture systems provide in vitro models for studying the opposing T-cell mechanisms involved in mycobacterial survival and protective host immunity... - Enhancement of innate and cell-mediated immunity by antimycobacterial antibodiesS de Valliere
Department of Internal Medicine, Saint Louis University Health Science Center, MO 63110, USA
Infect Immun 73:6711-20. 2005..We conclude that mycobacterium-specific antibodies are capable of enhancing both innate and cell-mediated immune responses to mycobacteria... - Gastric invasion by Trypanosoma cruzi and induction of protective mucosal immune responsesD F Hoft
Department of Internal Medicine, Saint Louis University Health Sciences Center, Missouri 63110, USA
Infect Immun 64:3800-10. 1996..Our results document the first example of systemic microbial invasion through gastric mucosa and suggest the feasibility of a mucosal vaccine designed to prevent infection with this important human pathogen... - Immunoregulatory role of secreted glycoprotein G from respiratory syncytial virusR Ray
Division of Infectious Diseases and Immunology, Saint Louis University, 3635 Vista Avenue, St Louis, MO 63110, USA
Virus Res 75:147-54. 2001..However, Gs did not inhibit live RSV specific T cell responses. These results suggest that Gs may suppress immune response to unrelated antigens, but should not interfere with the overall development of RSV specific immunity... - Anatomical route of invasion and protective mucosal immunity in Trypanosoma cruzi conjunctival infectionO K Giddings
Department of Molecular Microbiology and Immunology, St. Louis University Health Science Center, 3635 Vista Ave, FDT-8N, St. Louis, MO 63110, USA
Infect Immun 74:5549-60. 2006..05), demonstrating that mucosal antibodies can play a role in T. cruzi immunity. This model provides an important tool for detailed studies of mucosal immunity necessary for the development of mucosal vaccines... - Canarypox vaccines induce antigen-specific human gammadelta T cells capable of interferon-gamma productionS Worku
Division of Infectious Diseases and Immunology, Department of Internal Medicine, Saint Louis University Health Sciences Center, St Louis, Missouri, USA
J Infect Dis 184:525-32. 2001..02). Increased IFN-gamma production by gammadelta T and NK cells could enhance the induction of protective type 1 memory immunity. Thus, stimulation of gammadelta T cells might be an important feature of live vaccines... - Differential interleukin-8 and nitric oxide production in epithelial cells induced by mucosally invasive and noninvasive Trypanosoma cruzi trypomastigotesC S Eickhoff
Department of Internal Medicine, Saint Louis University Health Sciences Center, St Louis, Missouri, USA
Infect Immun 71:5394-7. 2003..MT may avoid induction of inflammation as an important biological mechanism facilitating mucosal invasion... - Induction of B- and T-cell responses to cruzipain in the murine model of Trypanosoma cruzi infectionAnita R Schnapp
Division of Infectious Diseases and Immunology, Department of Internal Medicine, St. Louis University Health Sciences Center, 3635 Vista Avenue, St. Louis, MO 63110, USA
Microbes Infect 4:805-13. 2002..cruzi-infected cells. The induction of serum antibody, mucosal IgA, Th1 cytokine and CTL responses by cruzipain in mice supports the use of this parasite protein for further efforts in T. cruzi vaccine development... - Cruzipain induces both mucosal and systemic protection against Trypanosoma cruzi in miceAnita R Schnapp
Department of Internal Medicine, St. Louis University Health Sciences Center, St. Louis, Missouri 63110, USA
Infect Immun 70:5065-74. 2002..cruzi mucosal infection. Overall, these data indicate that cruzipain is an important T. cruzi vaccine candidate and that protective T. cruzi vaccines will need to induce more than CD4(+) Th1 cells alone... - Bactericidal activity in whole blood as a potential surrogate marker of immunity after vaccination against tuberculosisSeon-Hee Cheon
Case Western Reserve University, Cleveland, Ohio, USA
Clin Diagn Lab Immunol 9:901-7. 2002..These experiments indicate that whole blood culture may be used to measure immunity to M. tuberculosis and that further studies of repeated BCG vaccination are warranted... - Flow-cytometric detection of vaccinia-induced memory effector CD4(+), CD8(+), and gamma delta TCR(+) T cells capable of antigen-specific expansion and effector functionsGetahun Abate
Department of Internal Medicine, Saint Louis University, St. Louis, Missouri 63110, USA
J Infect Dis 192:1362-71. 2005..CFSE-based flow-cytometric assays will be useful in evaluating cell-mediated immune responses induced by new smallpox vaccines... - CD8+-T-cell-dependent control of Trypanosoma cruzi infection in a highly susceptible mouse strain after immunization with recombinant proteins based on amastigote surface protein 2Adriano F S Araújo
CINTERGEN, UNIFESP Escola Paulista de Medicina, Rua Mirassol, 207, São Paulo SP 04044 010, Brazil
Infect Immun 73:6017-25. 2005..cruzi infection... - Hepatitis C virus inhibits cell surface expression of HLA-DR, prevents dendritic cell maturation, and induces interleukin-10 productionKousuke Saito
Departments of Internal Medicine, Saint Louis University, St Louis, Missouri, USA
J Virol 82:3320-8. 2008..These effects may account for or contribute to the low overall level of immunogenicity of HCV observed in chronically infected patients... - Lipoarabinomannan-reactive human secretory immunoglobulin A responses induced by mucosal bacille Calmette-Guérin vaccinationRobin M Brown
Saint Louis University Vaccine and Treatment Evaluation Unit, Division of Infectious Diseases and Immunology, Department of Internal Medicine, Saint Louis University, St. Louis, Missouri 63110, USA
J Infect Dis 187:513-7. 2003..05). LAM-reactive antibody assays can serve as markers of humoral and mucosal immunity in future trials of BCG and newer attenuated mycobacterial vaccines...
Research Grants
- Role of gamma/delta T cells in Vaccine Induced ImmunityDANIEL HOFT; Fiscal Year: 2009..Dobos at Colorado State, who will pursue detailed biochemical studies designed to identify the specific components of mycobacteria and vaccinia that induce gamma/delta T cells relevant for vaccine development. ..
- Role of gamma/delta T cells in Vaccine Induced ImmunityDANIEL HOFT; Fiscal Year: 2007..Dobos at Colorado State, who will pursue detailed biochemical studies designed to identify the specific components of mycobacteria and vaccinia that induce gamma/delta T cells relevant for vaccine development. ..
- TRYPANOSOMA CRUZI MUCOSAL & SYSTEMIC PROTECTIVE IMMUNITYDANIEL HOFT; Fiscal Year: 2007..Chemokine and/or integrin receptor expression will be studied by flow cytometry and RT-Real-Time PCR, and the in vivo effects of defective chemokine and integrin binding and/or signaling will be evaluated. ..
- Vaccine Induced Mucosal Protection Against TB InfectionDANIEL HOFT; Fiscal Year: 2005..Prime/boosting strategies found to prevent BCG infection after aerosol challenges will be studied for the ability to prevent infection with virulent M. tuberculosis by aerosol challenge. ..
- Role of gammadelta T Cells in Vaccine-Induced ImmunityDANIEL HOFT; Fiscal Year: 2004..Finally, the involvement of gd T cells in protective effector mechanisms capable of inhibiting intracellular replication of mycobacteria inside macrophages and HIV inside T cells will be examined. ..
- TRYPANOSOMA CRUZI MUCOSAL & SYSTEMIC PROTECTIVE IMMUNITYDANIEL HOFT; Fiscal Year: 2002..Protective soluble protein and live recombinant vaccines will be studied in genetic knockout mice to identify the molecular and cellular requirements for immune protection. ..
- Role of gamma/delta T cells in Vaccine Induced ImmunityDaniel F Hoft; Fiscal Year: 2010..Dobos at Colorado State, who will pursue detailed biochemical studies designed to identify the specific components of mycobacteria and vaccinia that induce gamma/delta T cells relevant for vaccine development. ..