ANN STOCK

Summary

Affiliation: Rutgers University
Country: USA

Publications

  1. pmc Regulation of response regulator autophosphorylation through interdomain contacts
    Christopher M Barbieri
    Center for Advanced Biotechnology and Medicine, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 5635, USA
    J Biol Chem 285:32325-35. 2010
  2. pmc Crystal structure and autoactivation pathway of the precursor form of human tripeptidyl-peptidase 1, the enzyme deficient in late infantile ceroid lipofuscinosis
    Jayita Guhaniyogi
    Center for Advanced Biotechnology and Medicine, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, New Jersey, USA
    J Biol Chem 284:3985-97. 2009
  3. pmc System-level mapping of Escherichia coli response regulator dimerization with FRET hybrids
    Rong Gao
    Center for Advanced Biotechnology and Medicine, Howard HughesMedical Institute, UMDNJ Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Mol Microbiol 69:1358-72. 2008
  4. pmc Evolutionary tuning of protein expression levels of a positively autoregulated two-component system
    Rong Gao
    Center for Advanced Biotechnology and Medicine, Department of Biochemistry and Molecular Biology, Rutgers University Robert Wood Johnson Medical School, Piscataway, New Jersey, United States of America
    PLoS Genet 9:e1003927. 2013
  5. ncbi request reprint Two-component signal transduction
    A M Stock
    Center for Advanced Biotechnology and Medicine and Howard Hughes Medical Institute, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Annu Rev Biochem 69:183-215. 2000
  6. pmc A new perspective on response regulator activation
    Ann M Stock
    Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854 5627, USA
    J Bacteriol 188:7328-30. 2006
  7. pmc Crystal structures of beryllium fluoride-free and beryllium fluoride-bound CheY in complex with the conserved C-terminal peptide of CheZ reveal dual binding modes specific to CheY conformation
    Jayita Guhaniyogi
    Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854, USA
    J Mol Biol 359:624-45. 2006
  8. pmc Interaction of CheY with the C-terminal peptide of CheZ
    Jayita Guhaniyogi
    Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854, USA
    J Bacteriol 190:1419-28. 2008
  9. pmc Biological insights from structures of two-component proteins
    Rong Gao
    Center for Advanced Biotechnology and Medicine, Department of Biochemistry, UMDNJ Robert Wood Johnson Medical School and Howard Hughes Medical Institute, Piscataway, New Jersey 08854 5627, USA
    Annu Rev Microbiol 63:133-54. 2009
  10. pmc Structure of the Staphylococcus aureus AgrA LytTR domain bound to DNA reveals a beta fold with an unusual mode of binding
    David J Sidote
    Center for Advanced Biotechnology and Medicine and Department of Biochemistry, Howard Hughes Medical Institute, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, 679 Hoes Lane, Piscataway, NJ 08854, USA
    Structure 16:727-35. 2008

Collaborators

Detail Information

Publications31

  1. pmc Regulation of response regulator autophosphorylation through interdomain contacts
    Christopher M Barbieri
    Center for Advanced Biotechnology and Medicine, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 5635, USA
    J Biol Chem 285:32325-35. 2010
    ....
  2. pmc Crystal structure and autoactivation pathway of the precursor form of human tripeptidyl-peptidase 1, the enzyme deficient in late infantile ceroid lipofuscinosis
    Jayita Guhaniyogi
    Center for Advanced Biotechnology and Medicine, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, New Jersey, USA
    J Biol Chem 284:3985-97. 2009
    ..These data provide new insights into TPP1 function and represent a valuable resource for constructing improved TPP1 variants for treatment of late infantile neuronal ceroid lipofuscinosis...
  3. pmc System-level mapping of Escherichia coli response regulator dimerization with FRET hybrids
    Rong Gao
    Center for Advanced Biotechnology and Medicine, Howard HughesMedical Institute, UMDNJ Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Mol Microbiol 69:1358-72. 2008
    ..Weak hetero-pair interactions were also identified between several different RRs, suggesting potential cross-regulation between distinct pathways...
  4. pmc Evolutionary tuning of protein expression levels of a positively autoregulated two-component system
    Rong Gao
    Center for Advanced Biotechnology and Medicine, Department of Biochemistry and Molecular Biology, Rutgers University Robert Wood Johnson Medical School, Piscataway, New Jersey, United States of America
    PLoS Genet 9:e1003927. 2013
    ..Our results suggest that the natural protein expression levels and feedback regulatory schemes of TCSs are evolved to match the phosphorylation output of the system, which is determined by intrinsic activities of TCS proteins. ..
  5. ncbi request reprint Two-component signal transduction
    A M Stock
    Center for Advanced Biotechnology and Medicine and Howard Hughes Medical Institute, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Annu Rev Biochem 69:183-215. 2000
    ..Thus detailed analyses of a relatively small number of representative proteins provide a foundation for understanding this large family of signaling proteins...
  6. pmc A new perspective on response regulator activation
    Ann M Stock
    Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854 5627, USA
    J Bacteriol 188:7328-30. 2006
  7. pmc Crystal structures of beryllium fluoride-free and beryllium fluoride-bound CheY in complex with the conserved C-terminal peptide of CheZ reveal dual binding modes specific to CheY conformation
    Jayita Guhaniyogi
    Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854, USA
    J Mol Biol 359:624-45. 2006
    ..Our dual binding mode hypothesis provides implications for reverse information flow in CheY and extends previous observations on inherent resilience in CheY-like signaling domains...
  8. pmc Interaction of CheY with the C-terminal peptide of CheZ
    Jayita Guhaniyogi
    Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854, USA
    J Bacteriol 190:1419-28. 2008
    ....
  9. pmc Biological insights from structures of two-component proteins
    Rong Gao
    Center for Advanced Biotechnology and Medicine, Department of Biochemistry, UMDNJ Robert Wood Johnson Medical School and Howard Hughes Medical Institute, Piscataway, New Jersey 08854 5627, USA
    Annu Rev Microbiol 63:133-54. 2009
    ....
  10. pmc Structure of the Staphylococcus aureus AgrA LytTR domain bound to DNA reveals a beta fold with an unusual mode of binding
    David J Sidote
    Center for Advanced Biotechnology and Medicine and Department of Biochemistry, Howard Hughes Medical Institute, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, 679 Hoes Lane, Piscataway, NJ 08854, USA
    Structure 16:727-35. 2008
    ..Loss of DNA binding upon substitution of key interacting residues in AgrA supports the observed binding mode. This mode of protein-DNA interaction provides a potential target for future antimicrobial drug design...
  11. ncbi request reprint Kinetic basis for the stimulatory effect of phosphorylation on the methylesterase activity of CheB
    Ganesh S Anand
    Center for Advanced Biotechnology and Medicine, Howard Hughes Medical Institute, Joint Program in Biochemistry, Rutgers University UMDNJ Robert Wood Johnson Medical School, 679 Hoes Lane, Piscataway, NJ 08854, USA
    Biochemistry 41:6752-60. 2002
    ..Finally, studies of methylesterase catalysis by the free catalytic domain in the presence and absence of the regulatory domain have enabled detection of an association between the two domains in the absence of the linker...
  12. pmc Crystal structures of the receiver domain of the response regulator PhoP from Escherichia coli in the absence and presence of the phosphoryl analog beryllofluoride
    Priti Bachhawat
    Department of Biochemistry, Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854 5627, USA
    J Bacteriol 189:5987-95. 2007
    ..In the absence of activating agents, the PhoP receiver domain crystallizes with a similar structure, consistent with the previous observation that high concentrations can promote an active state of PhoP independent of phosphorylation...
  13. pmc A common dimerization interface in bacterial response regulators KdpE and TorR
    Alejandro Toro-Roman
    Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854, USA
    Protein Sci 14:3077-88. 2005
    ..These results provide further evidence that most all response regulators of the OmpR/ PhoB subfamily share a common mechanism of activation by dimerization...
  14. pmc Molecular strategies for phosphorylation-mediated regulation of response regulator activity
    Rong Gao
    Center for Advanced Biotechnology and Medicine, UMDNJ Robert Wood Johnson, Medical School and Howard Hughes Medical Institute, Piscataway, NJ, USA
    Curr Opin Microbiol 13:160-7. 2010
    ..These themes involve interactions at the alpha4-beta5-alpha5 face of the receiver domain, modes of dimerization of receiver domains, and inhibitory or activating heterodomain interactions...
  15. pmc Probing the roles of the two different dimers mediated by the receiver domain of the response regulator PhoB
    Timothy R Mack
    Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854, USA
    J Mol Biol 389:349-64. 2009
    ..Together, these results support a model of activation of PhoB in which phosphorylation promotes dimerization via the alpha 4-beta 5-alpha 5 face, which enhances DNA binding and thus the ability of PhoB to regulate transcription...
  16. pmc Structural analysis of the domain interface in DrrB, a response regulator of the OmpR/PhoB subfamily
    Victoria L Robinson
    Howard Hughes Medical Institute, Center for Advanced Biotechnology and Medicine, and Department of Biochemistry, Robert Wood Johnson Medical School, The University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854, USA
    J Bacteriol 185:4186-94. 2003
    ....
  17. pmc Characterization of the Thermotoga maritima chemotaxis methylation system that lacks pentapeptide-dependent methyltransferase CheR:MCP tethering
    Eduardo Perez
    Center for Advanced Biotechnology and Medicine, UMDNJ Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Mol Microbiol 63:363-78. 2007
    ....
  18. pmc Structural analysis and solution studies of the activated regulatory domain of the response regulator ArcA: a symmetric dimer mediated by the alpha4-beta5-alpha5 face
    Alejandro Toro-Roman
    Department of Chemistry and Chemical Biology, Rutgers University, 610 Taylor Road, Piscataway, NJ 08854, USA
    J Mol Biol 349:11-26. 2005
    ....
  19. pmc Mechanism of activation for transcription factor PhoB suggested by different modes of dimerization in the inactive and active states
    Priti Bachhawat
    Center for Advanced Biotechnology and Medicine, Piscataway, NJ 08854, USA
    Structure 13:1353-63. 2005
    ....
  20. pmc Structure of a cholesterol-binding protein deficient in Niemann-Pick type C2 disease
    Natalia Friedland
    Center for Advanced Biotechnology and Medicine, Howard Hughes Medical Institute, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, 679 Hoes Lane, Piscataway, NJ 08854, USA
    Proc Natl Acad Sci U S A 100:2512-7. 2003
    ..We propose that this region represents the incipient cholesterol-binding site that dilates to accommodate an approximately 740-A(3) cholesterol molecule...
  21. ncbi request reprint Evidence of intradomain and interdomain flexibility in an OmpR/PhoB homolog from Thermotoga maritima
    David R Buckler
    Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854, USA
    Structure 10:153-64. 2002
    ..The lack of an extensive interface in the unphosphorylated protein distinguishes DrrD from other structurally characterized multidomain RRs and suggests a different mode of interdomain regulation...
  22. pmc Bacterial response regulators: versatile regulatory strategies from common domains
    Rong Gao
    Center for Advanced Biotechnology and Medicine, Howard Hughes Medical Institute, Piscataway, NJ 08854, USA
    Trends Biochem Sci 32:225-34. 2007
    ....
  23. pmc Universally applicable methods for monitoring response regulator aspartate phosphorylation both in vitro and in vivo using Phos-tag-based reagents
    Christopher M Barbieri
    Center for Advanced Biotechnology and Medicine, Department of Biochemistry and Howard Hughes Medical Institute, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Anal Biochem 376:73-82. 2008
    ....
  24. pmc Structural basis of sterol binding by NPC2, a lysosomal protein deficient in Niemann-Pick type C2 disease
    Sujuan Xu
    Center for Advanced Biotechnology and Medicine, Department of Biochemistry, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 282:23525-31. 2007
    ..The NPC2 structures provide evidence of a malleable binding site, consistent with the previously documented broad range of sterol ligand specificity...
  25. ncbi request reprint Domain arrangement of Der, a switch protein containing two GTPase domains
    Victoria L Robinson
    Howard Hughes Medical Institute and Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854, USA
    Structure 10:1649-58. 2002
    ..In contrast, the GD2-KH domain interface is distal to the GDP binding site of GD2...
  26. pmc Identification of methylation sites in Thermotoga maritima chemotaxis receptors
    Eduardo Perez
    Department of Biochemistry, Center for Advanced Biotechnology and Medicine, UMDNJ Robert Wood Johnson Medical School, 679 Hoes Lane, Piscataway, NJ 08854 5627, USA
    J Bacteriol 188:4093-100. 2006
    ..coli and S. enterica. These findings suggest that consensus sequences for posttranslational modifications in one organism may not be directly extrapolated to analogous modifications in other bacteria...
  27. pmc Discrimination between different methylation states of chemotaxis receptor Tar by receptor methyltransferase CheR
    Eduardo Perez
    Center for Advanced Biotechnology and Medicine, Department of Biochemistry, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Biochemistry 43:953-61. 2004
    ..It is likely that this complementarity is involved in discriminating different methylation states of the receptors...
  28. ncbi request reprint Molecular information processing: lessons from bacterial chemotaxis
    Robert B Bourret
    Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina 27599 7290, USA
    J Biol Chem 277:9625-8. 2002
  29. ncbi request reprint Diguanylate cyclase activation: it takes two
    Ann M Stock
    Structure 15:887-8. 2007
    ..2007) reveals how phosphorylation promotes dimerization necessary for synthesis of the second messenger c-di-GMP, establishes the catalytic mechanism, and identifies a widely conserved mode of product inhibition...
  30. pmc Domain orientation in the inactive response regulator Mycobacterium tuberculosis MtrA provides a barrier to activation
    Natalia Friedland
    Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
    Biochemistry 46:6733-43. 2007
    ..The domain orientation exhibited by MtrA also provides a rationale for the variation in linker length that is observed within the OmpR/PhoB family of response regulators...
  31. ncbi request reprint Transmembrane signaling by asymmetry
    Ann M Stock
    Nat Struct Mol Biol 13:862-3. 2006

Research Grants11

  1. STRUCTURE/FUNCTION OF RESPONSE REGULATOR PROTEINS
    ANN STOCK; Fiscal Year: 2001
    ..4. Structural analysis of a histidine protein kinase. The X-ray crystal structure of the cytoplasmic region of a thermostable histidine protein kinase, T. maritima HpkA, will be pursued. ..
  2. STRUCTURE/FUNCTION OF RESPONSE REGULATOR PROTEINS
    ANN STOCK; Fiscal Year: 2000
    ..4. Structural analysis of a histidine protein kinase. The X-ray crystal structure of the cytoplasmic region of a thermostable histidine protein kinase, T. maritima HpkA, will be pursued. ..
  3. STRUCTURE/FUNCTION OF RESPONSE REGULATOR PROTEINS
    ANN STOCK; Fiscal Year: 1999
    ..4. Structural analysis of a histidine protein kinase. The X-ray crystal structure of the cytoplasmic region of a thermostable histidine protein kinase, T. maritima HpkA, will be pursued. ..
  4. STRUCTURE AND FUNCTION OF RESPONSE REGULATOR PROTEINS
    ANN STOCK; Fiscal Year: 1993
    ....
  5. STRUCTURE AND FUNCTION OF RESPONSE REGULATOR PROTEINS
    ANN STOCK; Fiscal Year: 1992
    ....
  6. STRUCTURE AND FUNCTION OF RESPONSE REGULATOR PROTEINS
    ANN STOCK; Fiscal Year: 2007
    ..abstract_text> ..