Research Topics | ANN STOCKSummaryAffiliation: Rutgers University Country: USA Publications
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Publications
Two-component signal transductionA M Stock
Center for Advanced Biotechnology and Medicine and Howard Hughes Medical Institute, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
Annu Rev Biochem 69:183-215. 2000..Thus detailed analyses of a relatively small number of representative proteins provide a foundation for understanding this large family of signaling proteins...
A new perspective on response regulator activationAnn M Stock
Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854-5627, USA
J Bacteriol 188:7328-30. 2006
Biological insights from structures of two-component proteinsRong Gao
Center for Advanced Biotechnology and Medicine, Department of Biochemistry, UMDNJ Robert Wood Johnson Medical School and Howard Hughes Medical Institute, Piscataway, New Jersey 08854 5627, USA
Annu Rev Microbiol 63:133-54. 2009....- Crystal structures of beryllium fluoride-free and beryllium fluoride-bound CheY in complex with the conserved C-terminal peptide of CheZ reveal dual binding modes specific to CheY conformationJayita Guhaniyogi
Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854, USA
J Mol Biol 359:624-45. 2006..Our dual binding mode hypothesis provides implications for reverse information flow in CheY and extends previous observations on inherent resilience in CheY-like signaling domains... - Interaction of CheY with the C-terminal peptide of CheZJayita Guhaniyogi
Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854, USA
J Bacteriol 190:1419-28. 2008.... - System-level mapping of Escherichia coli response regulator dimerization with FRET hybridsRong Gao
Center for Advanced Biotechnology and Medicine, Howard HughesMedical Institute, UMDNJ Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Mol Microbiol 69:1358-72. 2008..Weak hetero-pair interactions were also identified between several different RRs, suggesting potential cross-regulation between distinct pathways... - Molecular strategies for phosphorylation-mediated regulation of response regulator activityRong Gao
Center for Advanced Biotechnology and Medicine, UMDNJ Robert Wood Johnson, Medical School and Howard Hughes Medical Institute, Piscataway, NJ, USA
Curr Opin Microbiol 13:160-7. 2010..These themes involve interactions at the alpha4-beta5-alpha5 face of the receiver domain, modes of dimerization of receiver domains, and inhibitory or activating heterodomain interactions... - Crystal structures of the receiver domain of the response regulator PhoP from Escherichia coli in the absence and presence of the phosphoryl analog beryllofluoridePriti Bachhawat
Department of Biochemistry, Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854 5627, USA
J Bacteriol 189:5987-95. 2007..In the absence of activating agents, the PhoP receiver domain crystallizes with a similar structure, consistent with the previous observation that high concentrations can promote an active state of PhoP independent of phosphorylation... - A common dimerization interface in bacterial response regulators KdpE and TorRAlejandro Toro-Roman
Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854, USA
Protein Sci 14:3077-88. 2005..These results provide further evidence that most all response regulators of the OmpR/ PhoB subfamily share a common mechanism of activation by dimerization... - Kinetic basis for the stimulatory effect of phosphorylation on the methylesterase activity of CheBGanesh S Anand
Center for Advanced Biotechnology and Medicine, Howard Hughes Medical Institute, Joint Program in Biochemistry, Rutgers University-UMDNJ-Robert Wood Johnson Medical School, 679 Hoes Lane, Piscataway, NJ 08854, USA
Biochemistry 41:6752-60. 2002..Finally, studies of methylesterase catalysis by the free catalytic domain in the presence and absence of the regulatory domain have enabled detection of an association between the two domains in the absence of the linker... - Structure of the Staphylococcus aureus AgrA LytTR domain bound to DNA reveals a beta fold with an unusual mode of bindingDavid J Sidote
Center for Advanced Biotechnology and Medicine and Department of Biochemistry, Howard Hughes Medical Institute, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, 679 Hoes Lane, Piscataway, NJ 08854, USA
Structure 16:727-35. 2008..Loss of DNA binding upon substitution of key interacting residues in AgrA supports the observed binding mode. This mode of protein-DNA interaction provides a potential target for future antimicrobial drug design... - Probing the roles of the two different dimers mediated by the receiver domain of the response regulator PhoBTimothy R Mack
Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854, USA
J Mol Biol 389:349-64. 2009..Together, these results support a model of activation of PhoB in which phosphorylation promotes dimerization via the alpha 4-beta 5-alpha 5 face, which enhances DNA binding and thus the ability of PhoB to regulate transcription... - Characterization of the Thermotoga maritima chemotaxis methylation system that lacks pentapeptide-dependent methyltransferase CheR:MCP tetheringEduardo Perez
Center for Advanced Biotechnology and Medicine, UMDNJ Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Mol Microbiol 63:363-78. 2007.... - Regulation of response regulator autophosphorylation through interdomain contactsChristopher M Barbieri
Center for Advanced Biotechnology and Medicine, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 5635, USA
J Biol Chem 285:32325-35. 2010.... - Structure of a cholesterol-binding protein deficient in Niemann-Pick type C2 diseaseNatalia Friedland
Center for Advanced Biotechnology and Medicine, Howard Hughes Medical Institute, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, 679 Hoes Lane, Piscataway, NJ 08854, USA
Proc Natl Acad Sci U S A 100:2512-7. 2003..We propose that this region represents the incipient cholesterol-binding site that dilates to accommodate an approximately 740-A(3) cholesterol molecule... - Structural analysis and solution studies of the activated regulatory domain of the response regulator ArcA: a symmetric dimer mediated by the alpha4-beta5-alpha5 faceAlejandro Toro-Roman
Department of Chemistry and Chemical Biology, Rutgers University, 610 Taylor Road, Piscataway, NJ 08854, USA
J Mol Biol 349:11-26. 2005.... - Mechanism of activation for transcription factor PhoB suggested by different modes of dimerization in the inactive and active statesPriti Bachhawat
Center for Advanced Biotechnology and Medicine, Piscataway, NJ 08854, USA
Structure 13:1353-63. 2005.... - Structural analysis of the domain interface in DrrB, a response regulator of the OmpR/PhoB subfamilyVictoria L Robinson
Howard Hughes Medical Institute, Center for Advanced Biotechnology and Medicine, and Department of Biochemistry, Robert Wood Johnson Medical School, The University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854, USA
J Bacteriol 185:4186-94. 2003.... - Bacterial response regulators: versatile regulatory strategies from common domainsRong Gao
Center for Advanced Biotechnology and Medicine, Howard Hughes Medical Institute, Piscataway, NJ 08854, USA
Trends Biochem Sci 32:225-34. 2007.... - Domain arrangement of Der, a switch protein containing two GTPase domainsVictoria L Robinson
Howard Hughes Medical Institute and Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854, USA
Structure 10:1649-58. 2002..In contrast, the GD2-KH domain interface is distal to the GDP binding site of GD2... - Structural basis of sterol binding by NPC2, a lysosomal protein deficient in Niemann-Pick type C2 diseaseSujuan Xu
Center for Advanced Biotechnology and Medicine, Department of Biochemistry, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
J Biol Chem 282:23525-31. 2007..The NPC2 structures provide evidence of a malleable binding site, consistent with the previously documented broad range of sterol ligand specificity... - Evidence of intradomain and interdomain flexibility in an OmpR/PhoB homolog from Thermotoga maritimaDavid R Buckler
Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854, USA
Structure 10:153-64. 2002..The lack of an extensive interface in the unphosphorylated protein distinguishes DrrD from other structurally characterized multidomain RRs and suggests a different mode of interdomain regulation... - Universally applicable methods for monitoring response regulator aspartate phosphorylation both in vitro and in vivo using Phos-tag-based reagentsChristopher M Barbieri
Center for Advanced Biotechnology and Medicine, Department of Biochemistry and Howard Hughes Medical Institute, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Anal Biochem 376:73-82. 2008.... - Crystal structure and autoactivation pathway of the precursor form of human tripeptidyl-peptidase 1, the enzyme deficient in late infantile ceroid lipofuscinosisJayita Guhaniyogi
Center for Advanced Biotechnology and Medicine, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, New Jersey, USA
J Biol Chem 284:3985-97. 2009..These data provide new insights into TPP1 function and represent a valuable resource for constructing improved TPP1 variants for treatment of late infantile neuronal ceroid lipofuscinosis... - Discrimination between different methylation states of chemotaxis receptor Tar by receptor methyltransferase CheREduardo Perez
Center for Advanced Biotechnology and Medicine, Department of Biochemistry, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
Biochemistry 43:953-61. 2004..It is likely that this complementarity is involved in discriminating different methylation states of the receptors... - Identification of methylation sites in Thermotoga maritima chemotaxis receptorsEduardo Perez
Department of Biochemistry, Center for Advanced Biotechnology and Medicine, UMDNJ Robert Wood Johnson Medical School, 679 Hoes Lane, Piscataway, NJ 08854 5627, USA
J Bacteriol 188:4093-100. 2006..coli and S. enterica. These findings suggest that consensus sequences for posttranslational modifications in one organism may not be directly extrapolated to analogous modifications in other bacteria... - Molecular information processing: lessons from bacterial chemotaxisRobert B Bourret
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina 27599-7290, USA
J Biol Chem 277:9625-8. 2002 - Diguanylate cyclase activation: it takes twoAnn M Stock
Structure 15:887-8. 2007..2007) reveals how phosphorylation promotes dimerization necessary for synthesis of the second messenger c-di-GMP, establishes the catalytic mechanism, and identifies a widely conserved mode of product inhibition... - Domain orientation in the inactive response regulator Mycobacterium tuberculosis MtrA provides a barrier to activationNatalia Friedland
Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
Biochemistry 46:6733-43. 2007..The domain orientation exhibited by MtrA also provides a rationale for the variation in linker length that is observed within the OmpR/PhoB family of response regulators... - Transmembrane signaling by asymmetryAnn M Stock
Nat Struct Mol Biol 13:862-3. 2006
Research Grants
- STRUCTURE/FUNCTION OF RESPONSE REGULATOR PROTEINSANN STOCK; Fiscal Year: 2001..4. Structural analysis of a histidine protein kinase. The X-ray crystal structure of the cytoplasmic region of a thermostable histidine protein kinase, T. maritima HpkA, will be pursued. ..
- STRUCTURE AND FUNCTION OF RESPONSE REGULATOR PROTEINSANN STOCK; Fiscal Year: 1993....
- STRUCTURE AND FUNCTION OF RESPONSE REGULATOR PROTEINSANN STOCK; Fiscal Year: 2007..abstract_text> ..