Research Topics
Genomes and Genes | Sidney PestkaSummaryAffiliation: Rutgers University Country: USA Publications
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Publications
Site-specific 32P-labeling of cytokines, monoclonal antibodies, and other protein substrates for quantitative assays and therapeutic applicationWilliam A Clark
PBL Biomedical Laboratories, Piscataway, NJ 08854 5900, USA
Biotechniques . 2002..Most importantly 32P labeling of monoclonal antibodies or other therapeutic protein candidates has several significant advantages over radioiodination or chemical conjugation of heavy metal isotopes...- Interferons, interferon-like cytokines, and their receptorsSidney Pestka
Department of Molecular Genetics, Microbiology, and Immunology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, NJ 08854 5635, USA
Immunol Rev 202:8-32. 2004..The overall structure of the IFNs is put into perspective in relation to their receptors and functions... - Interleukin-10 and related cytokines and receptorsSidney Pestka
Department of Molecular Genetics, Microbiology, and Immunology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854, USA
Annu Rev Immunol 22:929-79. 2004..Further investigation of these critical intercellular signaling molecules will provide important information to enable these proteins to be used more extensively in therapy for a variety of diseases... - A dance between interferon-alpha/beta and p53 demonstrates collaborations in tumor suppression and antiviral activitiesSidney Pestka
Department of Molecular Genetics, Microbiology and Immunology, Robert Wood Johnson Medical School UMDNJ, 675 Hoes Lane, Piscataway, NJ 08854, USA
Cancer Cell 4:85-7. 2003.... - Protein arginine methyltransferases: evolution and assessment of their pharmacological and therapeutic potentialChristopher D Krause
Department of Molecular Genetics, Microbiology, and Immunology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Pharmacol Ther 113:50-87. 2007..Nevertheless, development of pharmaceutical agents to control PRMT functions could lead to significant new targets... 
Chaperone Hsp27 modulates AUF1 proteolysis and AU-rich element-mediated mRNA degradationAnna M Knapinska
Department of Molecular Genetics, Microbiology and Immunology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 675 Hoes Lane West, Piscataway, NJ 08854, USA
Mol Cell Biol 31:1419-31. 2011..Our results suggest that the p38 MAP kinase (MAPK)-MK2-Hsp27 signaling axis may target AUF1 destruction by proteasomes, thereby promoting ARE mRNA stabilization...- Preassembly and ligand-induced restructuring of the chains of the IFN-gamma receptor complex: the roles of Jak kinases, Stat1 and the receptor chainsChristopher D Krause
Department of Molecular Genetics, Microbiology and Immunology, The University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, 675 Hoes Lane West, Piscataway, 08854, USA
Cell Res 16:55-69. 2006..These results agree with a detailed model of the IFN-gamma receptor that requires the receptor chains to be pre-associated constitutively for the receptor to be active... - Interactions among the components of the interleukin-10 receptor complexChristopher D Krause
Department of Molecular Genetics, Microbiology, and Immunology, The University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, 675 Hoes Lane West, Piscataway, NJ 08854, USA
Biochem Biophys Res Commun 340:377-85. 2006..The IL-10 receptor complex undergoes no major conformational changes when cells are treated with cellular or Epstein-Barr viral IL-10. Receptor complex preassembly may be an inherent feature of Class 2 cytokine receptor complexes... - RNA-binding protein AUF1 regulates lipopolysaccharide-induced IL10 expression by activating IkappaB kinase complex in monocytesSrijata Sarkar
Department of Molecular Genetics, Microbiology and Immunology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, NJ 08854 5635, USA
Mol Cell Biol 31:602-15. 2011..Thus, p40(AUF1) regulates a critical node within the NF-κB signaling pathway to permit IL10 induction for the anti-inflammatory arm of an innate immune response... - AUF1 isoform-specific regulation of anti-inflammatory IL10 expression in monocytesSrijata Sarkar
Department of Molecular Genetics, Microbiology, and Immunology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
J Interferon Cytokine Res 28:679-91. 2008..Thus, the p40 AUF1 isoform selectively plays a critical, positive role in IL10 expression upon LPS exposure... - Evolution of the Class 2 cytokines and receptors, and discovery of new friends and relativesChristopher D Krause
Department of Molecular Genetics, Microbiology and Immunology, Robert Wood Johnson Medical School University of Medicine and Dentistry of New Jersey, Piscataway, NJ 08854, USA
Pharmacol Ther 106:299-346. 2005..In addition, the genomic analysis led to the discovery of some new members of this family... - Design and construction of a phosphorylatable chimeric monoclonal antibody with a highly stable phosphateWei Wu
Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854-5635, USA
Oncol Res 14:541-58. 2004..The improved stability of the attached phosphate provides a MAb with potential to be used in diagnosis and therapy of adenocarcinomas... - Chaperone Hsp27, a novel subunit of AUF1 protein complexes, functions in AU-rich element-mediated mRNA decayKristina S Sinsimer
Department of Molecular Genetics, Microbiology and Immunology, UMDNJ Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
Mol Cell Biol 28:5223-37. 2008.... - Historical developments in the research of interferon receptorsChristopher D Krause
Department of Molecular Genetics, Microbiology, and Immunology, Robert Wood Johnson Medical School The University of Medicine and Dentistry of New Jersey, Piscataway, NJ 08854, USA
Cytokine Growth Factor Rev 18:473-82. 2007..In this mini-review, we take a historical perspective at the major events in the characterization of interferon receptors, discussing interesting results that still need to be explained... - Modulation of the activation of Stat1 by the interferon-gamma receptor complexChristopher D Krause
Department of Molecular Genetics, Microbiology and Immunology, Robert Wood Johnson Medical School, The University of Medicine and Dentistry of New Jersey, 675 Hoes Lane West, Piscataway, 08855, USA
Cell Res 16:113-23. 2006.... - Efficient co-expression of bicistronic proteins in mesenchymal stem cells by development and optimization of a multifunctional plasmidChristopher D Krause
Department of Molecular Genetics, Microbiology, and Immunology, RWJMS UMDNJ, 675 Hoes Lane West, Piscataway, NJ 08854, USA
Stem Cell Res Ther 2:15. 2011..Many vectors exist to fulfill this purpose, but their transfection efficiency and resulting expression levels vary considerably... - Seeing the light: preassembly and ligand-induced changes of the interferon gamma receptor complex in cellsChristopher D Krause
Department of Molecular Genetics, Microbiology and Immunology, Robert Wood Johnson Medical School University of Medicine and Dentistry of New Jersey, Piscataway 08854 5635, USA
Mol Cell Proteomics 1:805-15. 2002..Application of this technology should lead to new rapid methods for high throughput screening and delineation of the interactome of cells... - FBXO11/PRMT9, a new protein arginine methyltransferase, symmetrically dimethylates arginine residuesJeffry R Cook
UMDNJ Robert Wood Johnson Medical School, Molecular Genetics and Immunology, 675 Hoes Lane, Rm 727, Piscataway, NJ 08854, USA
Biochem Biophys Res Commun 342:472-81. 2006..Structurally, this protein is distinct from all other known PRMTs implying that convergent evolution allowed this protein to develop the ability to methylate arginine residues and evolved elements conserved in PRMTs to accomplish this... - Elevated expression of ISG15 in tumor cells interferes with the ubiquitin/26S proteasome pathwayShyamal D Desai
Department of Pharmacology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
Cancer Res 66:921-8. 2006..Together, our results suggest that the ISG15 pathway, which is deregulated during tumorigenesis, negatively regulates the ubiquitin/proteasome pathway by interfering with protein polyubiquitination/degradation... - The interferons: 50 years after their discovery, there is much more to learnSidney Pestka
Department of Molecular Genetics, Microbiology, and Immunology, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
J Biol Chem 282:20047-51. 2007..This brief review focuses on the discovery and purification of interferons, cloning of human IFN-alpha and IFN-beta, interferon receptors, activities and therapeutic uses of interferons, and the side effects of interferons... - Increased interleukin-10 mRNA stability in melanoma cells is associated with decreased levels of A + U-rich element binding factor AUF1Gary Brewer
Department of Molecular Genetics, Microbiology, and Immunology, Robert Wood Johnson Medical School UMDNJ, Piscataway, NJ 08854, USA
J Interferon Cytokine Res 23:553-64. 2003..Together, these data suggest a mechanism whereby reduced cytoplasmic levels of AUF1 in MNT1 melanoma cells may lead to IL-10 overexpression, with deleterious consequences for tumor surveillance and rejection... - The antineoplastic agent bryostatin-1 differentially regulates IFN-gamma receptor subunits in monocytic cells: transcriptional and posttranscriptional control of IFN-gamma R2Carmen S Garcia
Department of Medicine and Stanley S. Scott Cancer Center, Louisiana State University Medical Center, New Orleans, LA 70112, USA
J Immunol 177:2707-16. 2006.... - Expression analysis and genomic characterization of human melanoma differentiation associated gene-5, mda-5: a novel type I interferon-responsive apoptosis-inducing geneDong Chul Kang
Department of Pathology, Herbert Irving Comprehensive Cancer Center, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
Oncogene 23:1789-800. 2004..The conserved genomic and protein structure of mda-5 in human and mouse will permit analysis of the evolution and developmental aspects of this gene... - Mda-7/IL-24 induces apoptosis of diverse cancer cell lines through JAK/STAT-independent pathwaysMoira Sauane
Department of Pathology, Herbert Irving Comprehensive Cancer Center, Columbia University, College of Physicians and Surgeons, New York, New York, USA
J Cell Physiol 196:334-45. 2003..mda-7 induced apoptosis, might be tyrosine kinase independent and can thus be distinguished from its cytokine function related properties mediated by the IL-20/IL-22 receptor complexes that require JAK/STAT kinase activity... - MDA-7/IL-24: novel cancer growth suppressing and apoptosis inducing cytokineMoira Sauane
Department of Pathology, Herbert Irving Comprehensive Cancer Center, College of Physicians and Surgeons, Columbia University, BB 1501, 630 West 168th Street, New York, NY 10032, USA
Cytokine Growth Factor Rev 14:35-51. 2003..Various aspects of this intriguing molecule including its cytokine and anti-tumoral effects are described and discussed...
Research Grants
- VIRUS-HOST INTERACTIONS IN EUKARYOTIC CELLSSidney Pestka; Fiscal Year: 2007..Furthermore, the wide breadth of the investigators in this program provides its unique strength in developing genuinely novel and unanticipated advances in this area. ..