Sobin Kim

Summary

Affiliation: Rutgers University
Country: USA

Publications

  1. ncbi request reprint SNP genotyping: technologies and biomedical applications
    Sobin Kim
    Department of Biomedical Engineering, Rutgers University, Piscataway, New Jersey 08854, USA
    Annu Rev Biomed Eng 9:289-320. 2007
  2. doi request reprint Microbead device for isolating biotinylated oligonucleotides for use in mass spectrometric analysis
    Ashish Misra
    Department of Chemical and Biochemical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
    Anal Biochem 384:96-100. 2009
  3. ncbi request reprint Multiplex genotyping of cytochrome p450 single-nucleotide polymorphisms by use of MALDI-TOF mass spectrometry
    Ashish Misra
    Department of Chemical and Biochemical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
    Clin Chem 53:933-9. 2007
  4. pmc Simultaneous determination of multiple mRNA levels utilizing MALDI-TOF mass spectrometry and biotinylated dideoxynucleotides
    Daniel Scott Duffield
    Department of Chemical and Biochemical Engineering, Rutgers University, Piscataway, New Jersey 08854, USA
    RNA 16:1285-91. 2010
  5. pmc Solid phase capturable dideoxynucleotides for multiplex genotyping using mass spectrometry
    Sobin Kim
    Laboratory of DNA Sequencing and Chemical Biology, Columbia Genome Center, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Nucleic Acids Res 30:e85. 2002
  6. ncbi request reprint Digital genotyping using molecular affinity and mass spectrometry
    Sobin Kim
    Columbia Genome Center and the Department of Chemical Engineering, Columbia University College of Physicians and Surgeons, New York 10032, USA
    Nat Rev Genet 4:1001-8. 2003
  7. ncbi request reprint Multiplex genotyping of the human beta2-adrenergic receptor gene using solid-phase capturable dideoxynucleotides and mass spectrometry
    Sobin Kim
    Laboratory of DNA Sequencing and Chemical Biology, Columbia Genome Center, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Anal Biochem 316:251-8. 2003
  8. pmc Design and synthesis of a photocleavable biotinylated nucleotide for DNA analysis by mass spectrometry
    Xiaopeng Bai
    Laboratory of DNA Sequencing and Chemical Biology, Columbia Genome Center, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Nucleic Acids Res 32:535-41. 2004
  9. ncbi request reprint Thirtyfold multiplex genotyping of the p53 gene using solid phase capturable dideoxynucleotides and mass spectrometry
    Sobin Kim
    Columbia Genome Center, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Genomics 83:924-31. 2004
  10. pmc A photocleavable fluorescent nucleotide for DNA sequencing and analysis
    Zengmin Li
    Columbia Genome Center, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 100:414-9. 2003

Collaborators

  • Li Cai
  • Jun yan Hong
  • Ashish Misra
  • Jingyue Ju
  • Hameer Ruparel
  • Xiaopeng Bai
  • Zengmin Li
  • Daniel Scott Duffield
  • Nicholas J Turro
  • Michael E Ulz

Detail Information

Publications11

  1. ncbi request reprint SNP genotyping: technologies and biomedical applications
    Sobin Kim
    Department of Biomedical Engineering, Rutgers University, Piscataway, New Jersey 08854, USA
    Annu Rev Biomed Eng 9:289-320. 2007
    ..Further, several current biomedical applications of SNP genotyping are discussed...
  2. doi request reprint Microbead device for isolating biotinylated oligonucleotides for use in mass spectrometric analysis
    Ashish Misra
    Department of Chemical and Biochemical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
    Anal Biochem 384:96-100. 2009
    ..These results indicate that the microbead device is a low-cost tool that enhances sample cleanup prior to MS for SNP genotyping...
  3. ncbi request reprint Multiplex genotyping of cytochrome p450 single-nucleotide polymorphisms by use of MALDI-TOF mass spectrometry
    Ashish Misra
    Department of Chemical and Biochemical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
    Clin Chem 53:933-9. 2007
    ..Studies on these polymorphisms in research and diagnostic settings typically involve large-scale genotyping and hence require high-throughput assays...
  4. pmc Simultaneous determination of multiple mRNA levels utilizing MALDI-TOF mass spectrometry and biotinylated dideoxynucleotides
    Daniel Scott Duffield
    Department of Chemical and Biochemical Engineering, Rutgers University, Piscataway, New Jersey 08854, USA
    RNA 16:1285-91. 2010
    ..Thus the approach enhances the parallel analysis of multiple gene transcripts by MS. Utilizing the method we have simultaneously measured mRNA levels of four genes (Rho, Nrl, Hprt, and Lhx2) in mouse retinal tissue...
  5. pmc Solid phase capturable dideoxynucleotides for multiplex genotyping using mass spectrometry
    Sobin Kim
    Laboratory of DNA Sequencing and Chemical Biology, Columbia Genome Center, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Nucleic Acids Res 30:e85. 2002
    ..Using this method, we simultaneously distinguished six nucleotide variations on synthetic DNA templates mimicking mutations in the p53 gene and two disease-associated SNPs in the human hereditary hemochromatosis gene...
  6. ncbi request reprint Digital genotyping using molecular affinity and mass spectrometry
    Sobin Kim
    Columbia Genome Center and the Department of Chemical Engineering, Columbia University College of Physicians and Surgeons, New York 10032, USA
    Nat Rev Genet 4:1001-8. 2003
    ..Pilot studies show that these recently developed approaches allow unambiguous multiplex detection of heterozygous variants and the identification of deletion and insertion variants...
  7. ncbi request reprint Multiplex genotyping of the human beta2-adrenergic receptor gene using solid-phase capturable dideoxynucleotides and mass spectrometry
    Sobin Kim
    Laboratory of DNA Sequencing and Chemical Biology, Columbia Genome Center, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Anal Biochem 316:251-8. 2003
    ..Comparison of this approach with direct sequencing and the restriction fragment length polymorphism method indicated that the SPC-SBE method is superior for detecting nucleotide variations at known SNP sites...
  8. pmc Design and synthesis of a photocleavable biotinylated nucleotide for DNA analysis by mass spectrometry
    Xiaopeng Bai
    Laboratory of DNA Sequencing and Chemical Biology, Columbia Genome Center, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Nucleic Acids Res 32:535-41. 2004
    ..Furthermore, these nucleotide analogs should also be useful in isolating DNA-protein complexes under non-denaturing conditions...
  9. ncbi request reprint Thirtyfold multiplex genotyping of the p53 gene using solid phase capturable dideoxynucleotides and mass spectrometry
    Sobin Kim
    Columbia Genome Center, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Genomics 83:924-31. 2004
    ..This is the highest level of multiplex genotyping reported thus far using MS, indicating that the approach might be applicable to screening a repertoire of genotypes in candidate genes as potential disease markers...
  10. pmc A photocleavable fluorescent nucleotide for DNA sequencing and analysis
    Zengmin Li
    Columbia Genome Center, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 100:414-9. 2003
    ..These fluorescent tags can be removed easily by photocleavage after the identification of each nucleotide in the DNA sequencing-by-synthesis approach...
  11. pmc Digital detection of genetic mutations using SPC-sequencing
    Hameer Ruparel
    Laboratory of DNA Sequencing and Chemical Biology, Columbia Genome Center, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    Genome Res 14:296-300. 2004
    ..We have compared SPC-sequencing with electrophoresis-based sequencing in characterizing the above BRCA1 mutations, demonstrating the significant advantage offered by SPC-sequencing for the accurate identification of frameshift mutations...

Research Grants1

  1. Analysis of gene expression changes in breast cancer using MALDI-TOF MS
    Sobin Kim; Fiscal Year: 2007
    ..Therefore, the method will provide easy classification of breast cancer subtypes and accurate determination of progression stages, which is critical for individual patient assessment and for personalized treatment. ..