Dawn Brasaemle

Summary

Affiliation: Rutgers University
Country: USA

Publications

  1. ncbi request reprint Functional studies on native and mutated forms of perilipins. A role in protein kinase A-mediated lipolysis of triacylglycerols
    John T Tansey
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 8028, USA
    J Biol Chem 278:8401-6. 2003
  2. ncbi request reprint Cell biology. A metabolic push to proliferate
    Dawn L Brasaemle
    Department of Nutritional Sciences, Rutgers, State University of New Jersey, New Brunswick, NJ 08901, USA
    Science 313:1581-2. 2006
  3. pmc Packaging of fat: an evolving model of lipid droplet assembly and expansion
    Dawn L Brasaemle
    Rutgers Center for Lipid Research and Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA
    J Biol Chem 287:2273-9. 2012
  4. ncbi request reprint Developmental biology: holding pattern for histones
    Dawn L Brasaemle
    Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA
    Curr Biol 16:R918-20. 2006
  5. ncbi request reprint Proteomic analysis of proteins associated with lipid droplets of basal and lipolytically stimulated 3T3-L1 adipocytes
    Dawn L Brasaemle
    Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA
    J Biol Chem 279:46835-42. 2004
  6. ncbi request reprint Thematic review series: adipocyte biology. The perilipin family of structural lipid droplet proteins: stabilization of lipid droplets and control of lipolysis
    Dawn L Brasaemle
    Department of Nutritional Sciences and the Rutgers Center for Lipid Research, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA
    J Lipid Res 48:2547-59. 2007
  7. doi request reprint Perilipin A and the control of triacylglycerol metabolism
    Dawn L Brasaemle
    Department of Nutritional Sciences, Rutgers, The State University of New Jersey, 96 Lipman Drive, New Brunswick, NJ 08901, USA
    Mol Cell Biochem 326:15-21. 2009
  8. ncbi request reprint Lipolysis control: the plot thickens
    Dawn L Brasaemle
    Rutgers Center for Lipid Research and Department of Nutritional Sciences, Rutgers University, New Brunswick, NJ 08901, USA
    Cell Metab 11:173-4. 2010
  9. pmc DisseCCTing phospholipid function in lipid droplet dynamics
    Dawn L Brasaemle
    Rutgers Center for Lipid Research, Rutgers University, New Brunswick, NJ 08901, USA
    Cell Metab 14:437-8. 2011
  10. ncbi request reprint Hydrophobic sequences target and anchor perilipin A to lipid droplets
    Vidya Subramanian
    Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA
    J Lipid Res 45:1983-91. 2004

Research Grants

Collaborators

  • Rong Wang
  • Lawrence Shapiro
  • Jeffrey C Hansen
  • Diego A Moreno
  • Ilya Raskin
  • SUSAN FRIED
  • Philipp E Scherer
  • So Young Park
  • Perry E Bickel
  • Anne Garcia
  • Alex W Cohen
  • Vidya Subramanian
  • Michael P Lisanti
  • William Schubert
  • Anna Sekowski
  • Jorge M Caviglia
  • Nathan E Wolins
  • Amy Marcinkiewicz
  • Franco Capozza
  • Terence M Williams
  • Sucharita Bhattacharyya
  • John T Tansey
  • Deanna DiDonato
  • Anita M Brinker
  • Nikhil Toraskar
  • Terry C Yin
  • Janet D Sparks
  • Joseph L Dixon
  • Denise Gauthier
  • Linda A Jelicks
  • Jason K Kim
  • Terry P Combs
  • You Ree Cho
  • Carlos Gomez
  • Puneeth Iyengar
  • Georgia Dolios
  • Babak Razani
  • Martha W Love
  • Xiao Bo Wang
  • Alexis Rothenberg
  • Alan R Kimmel
  • Jennifer M Jones
  • Kathryn A Fraser
  • Constantine Londos
  • Kathryn E Davis
  • Anne M Huml
  • Rainbow Vogt

Detail Information

Publications22

  1. ncbi request reprint Functional studies on native and mutated forms of perilipins. A role in protein kinase A-mediated lipolysis of triacylglycerols
    John T Tansey
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 8028, USA
    J Biol Chem 278:8401-6. 2003
    ..Moreover, the data indicate that the unique C-terminal portion of perilipin A is responsible for its protection against lipolysis and that phosphorylation at the N-terminal PKA sites attenuates this protective effect...
  2. ncbi request reprint Cell biology. A metabolic push to proliferate
    Dawn L Brasaemle
    Department of Nutritional Sciences, Rutgers, State University of New Jersey, New Brunswick, NJ 08901, USA
    Science 313:1581-2. 2006
  3. pmc Packaging of fat: an evolving model of lipid droplet assembly and expansion
    Dawn L Brasaemle
    Rutgers Center for Lipid Research and Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA
    J Biol Chem 287:2273-9. 2012
    ..In this minireview, we focus on recent evidence supporting an endoplasmic reticulum origin for LD formation and discuss recent findings regarding LD maturation and fusion...
  4. ncbi request reprint Developmental biology: holding pattern for histones
    Dawn L Brasaemle
    Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA
    Curr Biol 16:R918-20. 2006
    ..New research on lipid droplets in Drosophila embryos has led to the surprising conclusion that these poorly understood organelles have a novel role as a regulated storage depot of maternally supplied proteins, particularly histones...
  5. ncbi request reprint Proteomic analysis of proteins associated with lipid droplets of basal and lipolytically stimulated 3T3-L1 adipocytes
    Dawn L Brasaemle
    Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA
    J Biol Chem 279:46835-42. 2004
    ....
  6. ncbi request reprint Thematic review series: adipocyte biology. The perilipin family of structural lipid droplet proteins: stabilization of lipid droplets and control of lipolysis
    Dawn L Brasaemle
    Department of Nutritional Sciences and the Rutgers Center for Lipid Research, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA
    J Lipid Res 48:2547-59. 2007
    ..A model is discussed whereby perilipin serves as a dynamic scaffold to coordinate the access of enzymes to the lipid droplet in a manner that is responsive to the metabolic status of the adipocyte...
  7. doi request reprint Perilipin A and the control of triacylglycerol metabolism
    Dawn L Brasaemle
    Department of Nutritional Sciences, Rutgers, The State University of New Jersey, 96 Lipman Drive, New Brunswick, NJ 08901, USA
    Mol Cell Biochem 326:15-21. 2009
    ..We hypothesize that perilipin A binds accessory proteins to provide assistance in carrying out these functions...
  8. ncbi request reprint Lipolysis control: the plot thickens
    Dawn L Brasaemle
    Rutgers Center for Lipid Research and Department of Nutritional Sciences, Rutgers University, New Brunswick, NJ 08901, USA
    Cell Metab 11:173-4. 2010
    ..In this issue of Cell Metabolism, Yang, et al. identify G0S2 as a regulator of the key enzyme, adipose triglyceride lipase...
  9. pmc DisseCCTing phospholipid function in lipid droplet dynamics
    Dawn L Brasaemle
    Rutgers Center for Lipid Research, Rutgers University, New Brunswick, NJ 08901, USA
    Cell Metab 14:437-8. 2011
    ....
  10. ncbi request reprint Hydrophobic sequences target and anchor perilipin A to lipid droplets
    Vidya Subramanian
    Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA
    J Lipid Res 45:1983-91. 2004
    ..The three hydrophobic sequences play a major role in targeting and anchoring perilipins to lipid droplets...
  11. ncbi request reprint The amino and carboxyl termini of perilipin a facilitate the storage of triacylglycerols
    Anne Garcia
    Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA
    J Biol Chem 279:8409-16. 2004
    ..We conclude that the amino and carboxyl termini are critical to the function of perilipin A in facilitating triacylglycerol storage...
  12. ncbi request reprint Inhibitory effects of grape seed extract on lipases
    Diego A Moreno
    Biotech Center, Cook College, Rutgers The State University of New Jersey, New Brunswick, New Jersey 08901 8520, USA
    Nutrition 19:876-9. 2003
    ....
  13. ncbi request reprint The phosphorylation of serine 492 of perilipin a directs lipid droplet fragmentation and dispersion
    Amy Marcinkiewicz
    Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA
    J Biol Chem 281:11901-9. 2006
    ..We conclude that protein kinase A-mediated phosphorylation of serine 492 of perilipin A drives the fragmentation and dispersion of lipid droplets...
  14. ncbi request reprint The central domain is required to target and anchor perilipin A to lipid droplets
    Anne Garcia
    Department of Nutritional Sciences, Rutgers, State University of New Jersey, New Brunswick, New Jersey 08901, USA
    J Biol Chem 278:625-35. 2003
    ..Thus, we conclude that the central 25% of the perilipin A sequence contains all of the amino acids necessary to target and anchor the protein to lipid droplets...
  15. ncbi request reprint Perilipin A mediates the reversible binding of CGI-58 to lipid droplets in 3T3-L1 adipocytes
    Vidya Subramanian
    Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA
    J Biol Chem 279:42062-71. 2004
    ..Thus, CGI-58 binds to perilipin A-coated lipid droplets in a manner that is dependent upon the metabolic status of the adipocyte and the activity of cAMP-dependent protein kinase...
  16. ncbi request reprint Fixation methods for the study of lipid droplets by immunofluorescence microscopy
    Deanna DiDonato
    Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA
    J Histochem Cytochem 51:773-80. 2003
    ..As more lipid droplet-associated proteins are discovered and studied, it is critical to use appropriate methods to avoid studying artifacts...
  17. pmc ABHD5/CGI-58 facilitates the assembly and secretion of apolipoprotein B lipoproteins by McA RH7777 rat hepatoma cells
    Jorge M Caviglia
    Rutgers Center for Lipid Research, Rutgers, The State University of New Jersey, USA
    Biochim Biophys Acta 1791:198-205. 2009
    ..These findings suggest that ABHD5 facilitates the use of lipid intermediates derived from lipolysis of stored triacylglycerols for the assembly of lipoproteins...
  18. doi request reprint Isolation of lipid droplets from cells by density gradient centrifugation
    Dawn L Brasaemle
    Rutgers, The State University of New Jersey, New Brunswick, New Jersey, USA
    Curr Protoc Cell Biol . 2006
    ..This unit provides protocols for isolating lipid droplets from mammalian cells by discontinuous density gradient centrifugation...
  19. ncbi request reprint Role of caveolin-1 in the modulation of lipolysis and lipid droplet formation
    Alex W Cohen
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Diabetes 53:1261-70. 2004
    ..Taken together, our data provide the first molecular genetic evidence that caveolin-1 plays a critical functional and structural role in the modulation of both lipid droplet biogenesis and metabolism in vivo...
  20. ncbi request reprint Caveolin-3 knockout mice show increased adiposity and whole body insulin resistance, with ligand-induced insulin receptor instability in skeletal muscle
    Franco Capozza
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    Am J Physiol Cell Physiol 288:C1317-31. 2005
    ..These findings may have clinical implications for the early diagnosis and treatment of caveolinopathies...
  21. ncbi request reprint Caveolin-1 expression is essential for proper nonshivering thermogenesis in brown adipose tissue
    Alex W Cohen
    Department of Molecular Pharmacology, The Albert Einstein Cancer Center, 1300 Morris Park Ave, Bronx, NY 10461, USA
    Diabetes 54:679-86. 2005
    ..Taken together, our data provide the first molecular genetic evidence that caveolin-1 plays a critical functional and structural role in the modulation of thermogenesis via an effect on lipid mobilization...
  22. ncbi request reprint A proposed model of fat packaging by exchangeable lipid droplet proteins
    Nathan E Wolins
    Department of Medicine, Division of Endocrinology, Metabolism and Lipid Research, Washington University School of Medicine, Saint Louis, MO 63110, USA
    FEBS Lett 580:5484-91. 2006
    ..Different tissues express different complements of these lipid droplet proteins. Thus, the tissue-specific complement of these proteins determines how tissues manage lipid stores...

Research Grants12

  1. Perilipin and cellular triacylglycerol metabolism
    Dawn L Brasaemle; Fiscal Year: 2010
    ....
  2. Perilipins and cellular triacyglycerol metabolism
    Dawn Brasaemle; Fiscal Year: 2009
    ..These studies will contribute to a long-term goal of defining the factors on lipid droplets that control adipocyte TAG metabolism. ..
  3. Lipid Droplets: Metabolic Consequences of Stored Neutral Lipids
    Dawn Brasaemle; Fiscal Year: 2007
    ....
  4. Perilipins and cellular triacyglycerol metabolism
    Dawn Brasaemle; Fiscal Year: 2007
    ..These studies will contribute to a long-term goal of defining the factors on lipid droplets that control adipocyte TAG metabolism. ..
  5. PERILIPINS AND CELLULAR TRIACYLGLYCEROL METABOLISM
    Dawn Brasaemle; Fiscal Year: 2003
    ..These aims will be tested by the expression of intact and mutant perilipin A in cultured cells that lack perilipins followed by assays for lipid storage and release, and the presence of the mutant perilipins on lipid droplets. ..
  6. Perilipins and cellular triacyglycerol metabolism
    Dawn L Brasaemle; Fiscal Year: 2010
    ..These studies will contribute to a long-term goal of defining the factors on lipid droplets that control adipocyte TAG metabolism. ..