Research Topics
Species | Dawn BrasaemleSummaryAffiliation: Rutgers University Country: USA Publications
Research Grants
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Detail Information
Publications
Functional studies on native and mutated forms of perilipins. A role in protein kinase A-mediated lipolysis of triacylglycerolsJohn T Tansey
Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892-8028, USA
J Biol Chem 278:8401-6. 2003..Moreover, the data indicate that the unique C-terminal portion of perilipin A is responsible for its protection against lipolysis and that phosphorylation at the N-terminal PKA sites attenuates this protective effect...- Cell biology. A metabolic push to proliferateDawn L Brasaemle
Department of Nutritional Sciences, Rutgers, State University of New Jersey, New Brunswick, NJ 08901, USA
Science 313:1581-2. 2006 
Packaging of fat: an evolving model of lipid droplet assembly and expansionDawn L Brasaemle
Rutgers Center for Lipid Research and Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA
J Biol Chem 287:2273-9. 2012..In this minireview, we focus on recent evidence supporting an endoplasmic reticulum origin for LD formation and discuss recent findings regarding LD maturation and fusion...- Developmental biology: holding pattern for histonesDawn L Brasaemle
Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA
Curr Biol 16:R918-20. 2006..New research on lipid droplets in Drosophila embryos has led to the surprising conclusion that these poorly understood organelles have a novel role as a regulated storage depot of maternally supplied proteins, particularly histones... - Proteomic analysis of proteins associated with lipid droplets of basal and lipolytically stimulated 3T3-L1 adipocytesDawn L Brasaemle
Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA
J Biol Chem 279:46835-42. 2004.... - Thematic review series: adipocyte biology. The perilipin family of structural lipid droplet proteins: stabilization of lipid droplets and control of lipolysisDawn L Brasaemle
Department of Nutritional Sciences and the Rutgers Center for Lipid Research, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA
J Lipid Res 48:2547-59. 2007..A model is discussed whereby perilipin serves as a dynamic scaffold to coordinate the access of enzymes to the lipid droplet in a manner that is responsive to the metabolic status of the adipocyte... 
Perilipin A and the control of triacylglycerol metabolismDawn L Brasaemle
Department of Nutritional Sciences, Rutgers, The State University of New Jersey, 96 Lipman Drive, New Brunswick, NJ 08901, USA
Mol Cell Biochem 326:15-21. 2009..We hypothesize that perilipin A binds accessory proteins to provide assistance in carrying out these functions...- Lipolysis control: the plot thickensDawn L Brasaemle
Rutgers Center for Lipid Research and Department of Nutritional Sciences, Rutgers University, New Brunswick, NJ 08901, USA
Cell Metab 11:173-4. 2010..In this issue of Cell Metabolism, Yang, et al. identify G0S2 as a regulator of the key enzyme, adipose triglyceride lipase... - DisseCCTing phospholipid function in lipid droplet dynamicsDawn L Brasaemle
Rutgers Center for Lipid Research, Rutgers University, New Brunswick, NJ 08901, USA
Cell Metab 14:437-8. 2011.... - Hydrophobic sequences target and anchor perilipin A to lipid dropletsVidya Subramanian
Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA
J Lipid Res 45:1983-91. 2004..The three hydrophobic sequences play a major role in targeting and anchoring perilipins to lipid droplets... - The amino and carboxyl termini of perilipin a facilitate the storage of triacylglycerolsAnne Garcia
Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA
J Biol Chem 279:8409-16. 2004..We conclude that the amino and carboxyl termini are critical to the function of perilipin A in facilitating triacylglycerol storage... - Inhibitory effects of grape seed extract on lipasesDiego A Moreno
Biotech Center, Cook College, Rutgers The State University of New Jersey, New Brunswick, New Jersey 08901 8520, USA
Nutrition 19:876-9. 2003.... - The phosphorylation of serine 492 of perilipin a directs lipid droplet fragmentation and dispersionAmy Marcinkiewicz
Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA
J Biol Chem 281:11901-9. 2006..We conclude that protein kinase A-mediated phosphorylation of serine 492 of perilipin A drives the fragmentation and dispersion of lipid droplets... - The central domain is required to target and anchor perilipin A to lipid dropletsAnne Garcia
Department of Nutritional Sciences, Rutgers, State University of New Jersey, New Brunswick, New Jersey 08901, USA
J Biol Chem 278:625-35. 2003..Thus, we conclude that the central 25% of the perilipin A sequence contains all of the amino acids necessary to target and anchor the protein to lipid droplets... - Perilipin A mediates the reversible binding of CGI-58 to lipid droplets in 3T3-L1 adipocytesVidya Subramanian
Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA
J Biol Chem 279:42062-71. 2004..Thus, CGI-58 binds to perilipin A-coated lipid droplets in a manner that is dependent upon the metabolic status of the adipocyte and the activity of cAMP-dependent protein kinase... - Fixation methods for the study of lipid droplets by immunofluorescence microscopyDeanna DiDonato
Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA
J Histochem Cytochem 51:773-80. 2003..As more lipid droplet-associated proteins are discovered and studied, it is critical to use appropriate methods to avoid studying artifacts... - ABHD5/CGI-58 facilitates the assembly and secretion of apolipoprotein B lipoproteins by McA RH7777 rat hepatoma cellsJorge M Caviglia
Rutgers Center for Lipid Research, Rutgers, The State University of New Jersey, USA
Biochim Biophys Acta 1791:198-205. 2009..These findings suggest that ABHD5 facilitates the use of lipid intermediates derived from lipolysis of stored triacylglycerols for the assembly of lipoproteins... - Isolation of lipid droplets from cells by density gradient centrifugationDawn L Brasaemle
Rutgers, The State University of New Jersey, New Brunswick, New Jersey, USA
Curr Protoc Cell Biol . 2006..This unit provides protocols for isolating lipid droplets from mammalian cells by discontinuous density gradient centrifugation... - Role of caveolin-1 in the modulation of lipolysis and lipid droplet formationAlex W Cohen
Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
Diabetes 53:1261-70. 2004..Taken together, our data provide the first molecular genetic evidence that caveolin-1 plays a critical functional and structural role in the modulation of both lipid droplet biogenesis and metabolism in vivo... - Caveolin-3 knockout mice show increased adiposity and whole body insulin resistance, with ligand-induced insulin receptor instability in skeletal muscleFranco Capozza
Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
Am J Physiol Cell Physiol 288:C1317-31. 2005..These findings may have clinical implications for the early diagnosis and treatment of caveolinopathies... - Caveolin-1 expression is essential for proper nonshivering thermogenesis in brown adipose tissueAlex W Cohen
Department of Molecular Pharmacology, The Albert Einstein Cancer Center, 1300 Morris Park Ave, Bronx, NY 10461, USA
Diabetes 54:679-86. 2005..Taken together, our data provide the first molecular genetic evidence that caveolin-1 plays a critical functional and structural role in the modulation of thermogenesis via an effect on lipid mobilization... - A proposed model of fat packaging by exchangeable lipid droplet proteinsNathan E Wolins
Department of Medicine, Division of Endocrinology, Metabolism and Lipid Research, Washington University School of Medicine, Saint Louis, MO 63110, USA
FEBS Lett 580:5484-91. 2006..Different tissues express different complements of these lipid droplet proteins. Thus, the tissue-specific complement of these proteins determines how tissues manage lipid stores...
Research Grants
- Perilipin and cellular triacylglycerol metabolismDawn L Brasaemle; Fiscal Year: 2010....
- Perilipins and cellular triacyglycerol metabolismDawn Brasaemle; Fiscal Year: 2009..These studies will contribute to a long-term goal of defining the factors on lipid droplets that control adipocyte TAG metabolism. ..
- Lipid Droplets: Metabolic Consequences of Stored Neutral LipidsDawn Brasaemle; Fiscal Year: 2007....
- Perilipins and cellular triacyglycerol metabolismDawn Brasaemle; Fiscal Year: 2007..These studies will contribute to a long-term goal of defining the factors on lipid droplets that control adipocyte TAG metabolism. ..
- PERILIPINS AND CELLULAR TRIACYLGLYCEROL METABOLISMDawn Brasaemle; Fiscal Year: 2003..These aims will be tested by the expression of intact and mutant perilipin A in cultured cells that lack perilipins followed by assays for lipid storage and release, and the presence of the mutant perilipins on lipid droplets. ..
- Perilipins and cellular triacyglycerol metabolismDawn L Brasaemle; Fiscal Year: 2010..These studies will contribute to a long-term goal of defining the factors on lipid droplets that control adipocyte TAG metabolism. ..