Research Topics
| Ramon GonzalezSummaryAffiliation: Rice University Country: USA Publications
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Detail Information
Publications
Efficient synthesis of L-lactic acid from glycerol by metabolically engineered Escherichia coliSuman Mazumdar
Department of Chemical and Biomolecular Engineering, Rice University, 6100 Main Street, MS 362, Houston, TX 77005, USA
Microb Cell Fact 12:7. 2013..This work reports the engineering of E. coli for the efficient conversion of glycerol into L-lactic acid (L-lactate)...- A novel data mining method to identify assay-specific signatures in functional genomic studiesDerrick K Rollins
Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa 50011, USA
BMC Bioinformatics 7:377. 2006..This article proposes a new and powerful PCA-based method for the identification of assay-specific gene signatures in FG studies... 
Production of ethanol from thin stillage by metabolically engineered Escherichia coliRamon Gonzalez
Department of Chemical and Biomolecular Engineering, Rice University, 6100 Main Street, MS 362, Houston, TX, 77005, USA
Biotechnol Lett 32:405-11. 2010..These results, along with the comparable performance of strain EH05 (pZSKLMgldA) in 0.5 and 5 l fermenters, indicate a great potential for the adoption of this process by the biofuels industry...- A new model for the anaerobic fermentation of glycerol in enteric bacteria: trunk and auxiliary pathways in Escherichia coliRamon Gonzalez
Department of Chemical and Biomolecular Engineering, Rice University, P O Box 1892, Houston, TX 77251 1892, USA
Metab Eng 10:234-45. 2008..These include high concentrations of potassium and phosphate, low concentrations of glycerol, alkaline pH, and closed cultivation systems that promote the accumulation of hydrogen gas... - Understanding and harnessing the microaerobic metabolism of glycerol in Escherichia coliGuyton Durnin
Department of Chemical and Biomolecular Engineering, Rice University, 6100 Main Street, MS 362, P O Box 1892, Houston, Texas 77251 1892, USA
Biotechnol Bioeng 103:148-61. 2009..Engineered strains efficiently produced ethanol and hydrogen and ethanol and formate from glycerol in a minimal medium without rich supplements... - Escherichia coli strains engineered for homofermentative production of D-lactic acid from glycerolSuman Mazumdar
Department of Chemical and Biomolecular Engineering, Rice University, 6100 Main St, MS 362, Houston, TX 77005, USA
Appl Environ Microbiol 76:4327-36. 2010..5 g/liter/h and 1.25 g/g cell mass/h, respectively. The engineered homolactic route generates 1 to 2 mol of ATP per mol of d-lactate and is redox balanced, thus representing a viable metabolic pathway... - Fermentative utilization of glycerol by Escherichia coli and its implications for the production of fuels and chemicalsAbhishek Murarka
Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX 77251 1892, USA
Appl Environ Microbiol 74:1124-35. 2008..The implications of our findings for the production of reduced chemicals and fuels were illustrated by coproducing ethanol plus formic acid and ethanol plus hydrogen from glycerol at yields approaching their theoretical maximum... - Quantitative analysis of the fermentative metabolism of glycerol in Escherichia coliAngela Cintolesi
Department of Chemical and Biomolecular Engineering, Rice University, 6100 Main Street, MS 362 Houston, Texas 77005, USA
Biotechnol Bioeng 109:187-98. 2012.... - Metabolic analysis of wild-type Escherichia coli and a pyruvate dehydrogenase complex (PDHC)-deficient derivative reveals the role of PDHC in the fermentative metabolism of glucoseAbhishek Murarka
Department of Chemical and Biomolecular Engineering, RiceUniversity, Houston, Texas 77005, USA
J Biol Chem 285:31548-58. 2010..A stoichiometric analysis of CO(2) generation via PDHC and ox-PPP revealed that the PDHC route is more carbon- and energy-efficient, in agreement with its beneficial role in cell growth... 
Anaerobic fermentation of glycerol by Escherichia coli: a new platform for metabolic engineeringYandi Dharmadi
Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX 77251 1892, USA
Biotechnol Bioeng 94:821-9. 2006..coli-based platform for the anaerobic production of reduced chemicals from glycerol at yields higher than those obtained from common sugars, such as glucose...- Metabolic engineering of Escherichia coli for the production of succinate from glycerolMatthew D Blankschien
Department of Chemical and Biomolecular Engineering, Rice University, 6100 Main Street, MS 362, P O Box 1892, Houston, TX 77005, USA
Metab Eng 12:409-19. 2010..g. antibiotics). Our biocatalysts demonstrated a maximum specific productivity of approximately 400 mg succinate/g cell/h and a yield of 0.69 g succinate/g glycerol, on par with the use of glucose as a feedstock... - Engineered reversal of the β-oxidation cycle for the synthesis of fuels and chemicalsClementina Dellomonaco
Department of Chemical and Biomolecular Engineering, Rice University, Houston, Texas 77005, USA
Nature 476:355-9. 2011..The ubiquitous nature of β-oxidation, aldehyde/alcohol dehydrogenase and thioesterase enzymes has the potential to enable the efficient synthesis of these products in other industrial organisms... - Metabolic flux analysis of wild-type Escherichia coli and mutants deficient in pyruvate-dissimilating enzymes during the fermentative metabolism of glucuronateAbhishek Murarka
Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX, USA
Microbiology 156:1860-72. 2010..Secondly, the NADH generated by PDH can be converted to NADPH by the action of transhydrogenases, thus serving as biosynthetic reducing power in the synthesis of building blocks and macromolecules... - Anaerobic fermentation of glycerol in Paenibacillus macerans: metabolic pathways and environmental determinantsAshutosh Gupta
Department of Chemical and Biomolecular Engineering, Rice University, 6100 Main Street, MS 362, Houston, TX 77005, USA
Appl Environ Microbiol 75:5871-83. 2009..Given the availability, low price, and high degree of reduction of glycerol, the high metabolic rates exhibited by P. macerans N234A are of paramount importance for the production of fuels and chemicals... - Engineering Escherichia coli for the efficient conversion of glycerol to ethanol and co-productsSyed Shams Yazdani
Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX, USA
Metab Eng 10:340-51. 2008..These yields and productivities are superior to those reported for the conversion of glycerol to ethanol-H(2) or ethanol-formate by other organisms and equivalent to those achieved in the production of ethanol from sugars using E. coli... - Engineered respiro-fermentative metabolism for the production of biofuels and biochemicals from fatty acid-rich feedstocksClementina Dellomonaco
Department of Chemical and Biomolecular Engineering, Rice University, 6100 Main Street, MS 362, Houston, TX 77005, USA
Appl Environ Microbiol 76:5067-78. 2010..coli. Finally, the synthesis of isopropanol and succinate was also demonstrated. The work reported here represents the first effort toward engineering microorganisms for the conversion of FAs to the aforementioned products... - Metabolic engineering of Escherichia coli for the production of 1,2-propanediol from glycerolJames M Clomburg
Department of Chemical and Biomolecular Engineering, Rice University, 6100 Main Street, MS 362, Houston, Texas 77005, USA
Biotechnol Bioeng 108:867-79. 2011..The titer and yield achieved in this study were favorable to those obtained with the use of E. coli for the production of 1,2-PDO from common sugars... - Biofuel production in Escherichia coli: the role of metabolic engineering and synthetic biologyJames M Clomburg
Department of Chemical and Biomolecular Engineering, Rice University, 6100 Main Street, MS 362, Houston, TX 77005, USA
Appl Microbiol Biotechnol 86:419-34. 2010.... - Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industrySyed Shams Yazdani
Department of Chemical and Biomolecular Engineering, Rice University, 6100 Main Street, MS 362, Houston, TX 77005, USA
Curr Opin Biotechnol 18:213-9. 2007..Special attention is given to products whose synthesis from glycerol would be advantageous when compared with their production from common sugars... - Elementary network reconstruction: a framework for the analysis of regulatory networks in biological systemsYandi Dharmadi
Department of Chemical and Biomolecular Engineering, Rice University, 6100 Main Street, MS 362, Houston, TX 77005, USA
J Theor Biol 263:499-509. 2010..Since functional genomics studies are mainly concerned with redistribution of species abundances in perturbed systems, ENR could be exploited in the system-level analysis of biological systems... - A Synthetic Biology Approach to Engineer a Functional Reversal of the β-Oxidation CycleJames M Clomburg
Department of Chemical and Biomolecular Engineering and Department of Bioengineering, Rice University, 6100 Main St, Houston, Texas 77005, United States
ACS Synth Biol 1:541-54. 2012..The well-defined and self-contained nature of each functional unit makes the engineered reversal of the β-oxidation cycle "chassis neutral" and hence transferrable to the host of choice for efficient fuel or chemical production... - The path to next generation biofuels: successes and challenges in the era of synthetic biologyClementina Dellomonaco
Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX, USA
Microb Cell Fact 9:3. 2010.... - Light-triggered biocatalysis using thermophilic enzyme-gold nanoparticle complexesMatthew D Blankschien
Department of Chemical and Biomolecular Engineering, Rice University, 6100 Main Street, Houston, Texas 77005 1892, USA
ACS Nano 7:654-63. 2013..This approach could significantly improve how biochemical pathways are controlled for in vitro and, quite possibly, in vivo use... - Anaerobic fermentation of glycerol: a platform for renewable fuels and chemicalsJames M Clomburg
Department of Chemical and Biomolecular Engineering, Rice University, 6100 Main Street, MS 362, Houston, TX 77005, USA
Trends Biotechnol 31:20-8. 2013..This review focuses on current engineering efforts as well as the challenges involved in the utilization of glycerol as a carbon source for the production of fuels and chemicals...