Victor W Rodwell

Summary

Affiliation: Purdue University
Country: USA

Publications

  1. pmc Inhibition of the class II HMG-CoA reductase of Pseudomonas mevalonii
    Matija Hedl
    Department of Biochemistry, Purdue University, 175 South University Street, West Lafayette, IN 47907 2063, USA
    Protein Sci 13:1693-7. 2004
  2. pmc Enterococcus faecalis 3-hydroxy-3-methylglutaryl coenzyme A synthase, an enzyme of isopentenyl diphosphate biosynthesis
    Autumn Sutherlin
    Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907 1153, USA
    J Bacteriol 184:4065-70. 2002
  3. ncbi request reprint Multienzyme mevalonate pathway bioreactor
    Autumn Sutherlin
    Department of Biochemistry, Purdue University, 175 South University Street, West Lafayette, Indiana 47907 2063, USA
    Biotechnol Bioeng 87:546-51. 2004
  4. ncbi request reprint X-ray crystal structures of HMG-CoA synthase from Enterococcus faecalis and a complex with its second substrate/inhibitor acetoacetyl-CoA
    C Nicklaus Steussy
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    Biochemistry 44:14256-67. 2005
  5. pmc Enterococcus faecalis mevalonate kinase
    Matija Hedl
    Department of Biochemistry, Purdue University, West Lafayette, IN 47907 2063, USA
    Protein Sci 13:687-93. 2004
  6. doi request reprint A novel role for coenzyme A during hydride transfer in 3-hydroxy-3-methylglutaryl-coenzyme A reductase
    C Nicklaus Steussy
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, United States
    Biochemistry 52:5195-205. 2013
  7. ncbi request reprint A structural limitation on enzyme activity: the case of HMG-CoA synthase
    Calvin N Steussy
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    Biochemistry 45:14407-14. 2006
  8. pmc Class II 3-hydroxy-3-methylglutaryl coenzyme A reductases
    Matija Hedl
    Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA
    J Bacteriol 186:1927-32. 2004
  9. pmc Enterococcus faecalis phosphomevalonate kinase
    Stephanie S Doun
    Department of Biochemistry, Purdue University, 175 South University Street, West Lafayette, Indiana 47907 2063, USA
    Protein Sci 14:1134-9. 2005
  10. pmc Enterococcus faecalis acetoacetyl-coenzyme A thiolase/3-hydroxy-3-methylglutaryl-coenzyme A reductase, a dual-function protein of isopentenyl diphosphate biosynthesis
    Matija Hedl
    Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907 1153, USA
    J Bacteriol 184:2116-22. 2002

Collaborators

  • Calvin N Steussy
  • Lydia Tabernero
  • Matija Hedl
  • Autumn Sutherlin
  • John W Burgner
  • Cynthia V Stauffacher
  • Stephanie S Doun
  • Jon A Friesen
  • Scott D Briggs
  • Kevin R Lehnbeuter
  • Michael N Gwynn
  • Marie Mazzulla
  • Damien McDevitt
  • E Imogen Wilding
  • Pamela Lane
  • Barbara Sanchez-Neri

Detail Information

Publications12

  1. pmc Inhibition of the class II HMG-CoA reductase of Pseudomonas mevalonii
    Matija Hedl
    Department of Biochemistry, Purdue University, 175 South University Street, West Lafayette, IN 47907 2063, USA
    Protein Sci 13:1693-7. 2004
    ....
  2. pmc Enterococcus faecalis 3-hydroxy-3-methylglutaryl coenzyme A synthase, an enzyme of isopentenyl diphosphate biosynthesis
    Autumn Sutherlin
    Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907 1153, USA
    J Bacteriol 184:4065-70. 2002
    ..60 +/- 0.02. The K(m) for the hydrolysis of acetyl-CoA was 10 microM. Coupled conversion of acetyl-CoA to mevalonate was demonstrated by using HMG-CoA synthase and acetoacetyl-CoA thiolase/HMG-CoA reductase from E. faecalis...
  3. ncbi request reprint Multienzyme mevalonate pathway bioreactor
    Autumn Sutherlin
    Department of Biochemistry, Purdue University, 175 South University Street, West Lafayette, Indiana 47907 2063, USA
    Biotechnol Bioeng 87:546-51. 2004
    ..Finally, linking a Ni(++) affinity support bioreactor to an HPLC-mass spectrometer would provide an experimental and pedagogical tool for study of metabolite flux and pool sizes of intermediates to model regulation in intact cells...
  4. ncbi request reprint X-ray crystal structures of HMG-CoA synthase from Enterococcus faecalis and a complex with its second substrate/inhibitor acetoacetyl-CoA
    C Nicklaus Steussy
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    Biochemistry 44:14256-67. 2005
    ..This is consistent with the kinetics of the reaction that have shown acetoacetyl-CoA to be a potent inhibitor of the overall reaction, and provides a starting point in the search for a small molecule inhibitor...
  5. pmc Enterococcus faecalis mevalonate kinase
    Matija Hedl
    Department of Biochemistry, Purdue University, West Lafayette, IN 47907 2063, USA
    Protein Sci 13:687-93. 2004
    ..1 mM (ATP), and 3.3 mM (Mg(2+)). Unlike mammalian mevalonate kinases, E. faecalis mevalonate kinase utilized all tested nucleoside triphosphates as phosphoryl donors. ADP, but not AMP, inhibited the reaction with a K(i) of 2.7 mM...
  6. doi request reprint A novel role for coenzyme A during hydride transfer in 3-hydroxy-3-methylglutaryl-coenzyme A reductase
    C Nicklaus Steussy
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, United States
    Biochemistry 52:5195-205. 2013
    ..Identification of this reaction intermediate provides a template for the development of an inhibitor that would act as an antibiotic effective against the HMG-CoA reductase of methicillin-resistant Staphylococcus aureus. ..
  7. ncbi request reprint A structural limitation on enzyme activity: the case of HMG-CoA synthase
    Calvin N Steussy
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    Biochemistry 45:14407-14. 2006
    ..In addition, the hydroxyl of Ser308 rotates 120 degrees to a position where it is able to stabilize the carbanion intermediate formed by the methyl group of the acetyl-S-enzyme during its condensation with acetoacetyl-CoA...
  8. pmc Class II 3-hydroxy-3-methylglutaryl coenzyme A reductases
    Matija Hedl
    Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA
    J Bacteriol 186:1927-32. 2004
  9. pmc Enterococcus faecalis phosphomevalonate kinase
    Stephanie S Doun
    Department of Biochemistry, Purdue University, 175 South University Street, West Lafayette, Indiana 47907 2063, USA
    Protein Sci 14:1134-9. 2005
    ..The specific activity of the purified enzyme was 3.9 micromol substrate converted per minute per milligram protein. Applications to an immobilized enzyme bioreactor and to drug screening and design are discussed...
  10. pmc Enterococcus faecalis acetoacetyl-coenzyme A thiolase/3-hydroxy-3-methylglutaryl-coenzyme A reductase, a dual-function protein of isopentenyl diphosphate biosynthesis
    Matija Hedl
    Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907 1153, USA
    J Bacteriol 184:2116-22. 2002
    ..Sequence comparisons with other HMG-CoA reductases suggest that the essential active-site histidine is His756. The mvaE gene product represents the first example of an HMG-CoA reductase fused to another enzyme...
  11. ncbi request reprint Crystal structure of a statin bound to a class II hydroxymethylglutaryl-CoA reductase
    Lydia Tabernero
    School of Biological Sciences, University of Manchester, Oxford Road, United Kingdom
    J Biol Chem 278:19933-8. 2003
    ..We suggest that these differences might be exploited to develop selective class II inhibitors for use as antibacterial agents against pathogenic microorganisms...
  12. pmc The 3-hydroxy-3-methylglutaryl coenzyme-A (HMG-CoA) reductases
    Jon A Friesen
    Department of Chemistry, Illinois State University, Normal, IL 61790 4160, USA
    Genome Biol 5:248. 2004
    ..Probably because of its critical role in cellular cholesterol homeostasis, mammalian HMG-CoA reductase is extensively regulated at the transcriptional, translational, and post-translational levels...