Research Topics
| Wen JiangSummaryAffiliation: Purdue University Country: USA Publications
Research Grants
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Detail Information
Publications
A graph theory method for determination of cryo-EM image focusesWen Jiang
Markey Center for Structural Biology, Department of Biological Sciences, Purdue University, 249 S Martin Jischke Drive, West Lafayette, IN 47906, USA
J Struct Biol 180:343-51. 2012..The new integrated focus determination method will improve reliability of automated focus determination for large-scale data processing that is increasingly common in the cryo-EM field...
Backbone structure of the infectious epsilon15 virus capsid revealed by electron cryomicroscopyWen Jiang
Markey Center for Structural Biology, Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
Nature 451:1130-4. 2008..Beyond epsilon15, this method potentially offers a new approach for modelling the backbone conformations of the protein subunits in other macromolecular assemblies at near-native solution states...- Exterior modification of a DNA tetrahedronChuan Zhang
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA
Chem Commun (Camb) 46:6792-4. 2010..This paper reports an introduction of extra structural features into self-assembled DNA polyhedra... - Conformational flexibility facilitates self-assembly of complex DNA nanostructuresChuan Zhang
Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA
Proc Natl Acad Sci U S A 105:10665-9. 2008..These results demonstrate that conformational flexibility could be explored to generate complex DNA nanostructures. The basic concept might be further extended to other biomacromolecular systems, such as RNA and proteins... - DNA-directed three-dimensional protein organizationChuan Zhang
Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA
Angew Chem Int Ed Engl 51:3382-5. 2012..This strategy was also applied to different 3D DNA nanostructures and different proteins... 
Controlling the chirality of DNA nanocagesChuan Zhang
Department of Chemistry, Purdue University, West Lafayette, IN 47907 USA
Angew Chem Int Ed Engl 51:7999-8002. 2012..The resulting DNA nanocages have been characterized with a variety of methods. Such chiral control could be useful for tuning the photonic/optical properties of DNA-templated nanostructures...- Symmetry controls the face geometry of DNA polyhedraChuan Zhang
Department of Chemistry, Markey Center for Structural Biology and Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
J Am Chem Soc 131:1413-5. 2009..As a demonstration, DNA cubes, whose each face contains four tiles, have been assembled through these approaches... - Synergistic self-assembly of RNA and DNA moleculesSeung Hyeon Ko
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA
Nat Chem 2:1050-5. 2010..The current strategy could enable the integration of the precise programmability of DNA with the rich functionality of RNA... - Visualization of uncorrelated, tandem symmetry mismatches in the internal genome packaging apparatus of bacteriophage T7Fei Guo
Markey Center for Structural Biology, Department of Biological Sciences, Purdue University, West Lafayette, IN 47907
Proc Natl Acad Sci U S A 110:6811-6. 2013..We also found a small tilt of the core stack relative to the icosahedral fivefold axis and propose that this tilt assists DNA spooling without tangling during packaging... - Reversibly switching the surface porosity of a DNA tetrahedronChuan Zhang
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA
J Am Chem Soc 134:11998-2001. 2012..This work may lead to the design and construction of stimuli-responsive nanocages that might find applications as smart materials... - Visualization of bacteriophage T3 capsids with DNA incompletely packaged in vivoPing an Fang
Markey Center for Structural Biology, Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
J Mol Biol 384:1384-99. 2008.... - Cryo-EM Structure of a Novel Calicivirus, Tulane VirusGuimei Yu
Markey Center for Structural Biology, Department of Biological Science, Purdue University, West Lafayette, Indiana, United States of America
PLoS ONE 8:e59817. 2013..Residues in the S-P1 hinge between the S and P domain may play a critical role in the flexibility of P domains of C/C dimers... - Hierarchical self-assembly of DNA into symmetric supramolecular polyhedraYu He
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA
Nature 452:198-201. 2008..We expect that our assembly strategy can be adapted to allow the fabrication of a range of relatively complex three-dimensional structures... - Cryoelectron microscopy of icosahedral virus particlesWen Jiang
Department of Biological Sciences, Purdue Univesity, IN, USA
Methods Mol Biol 369:345-63. 2007..In addition, we will describe how to extract useful structural information from the three-dimensional maps... - Genome sequence, structural proteins, and capsid organization of the cyanophage Syn5: a "horned" bacteriophage of marine synechococcusWelkin H Pope
Department of Biology, Massachusetts Institute of Technology, Cambridge MA 02139, USA
J Mol Biol 368:966-81. 2007..This phage/host system should allow detailed investigation of the physiology and biochemistry of phage propagation in marine photosynthetic bacteria... - Structure of epsilon15 bacteriophage reveals genome organization and DNA packaging/injection apparatusWen Jiang
National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
Nature 439:612-6. 2006..The image reconstruction approach should be applicable to studying other biological nanomachines with components of mixed symmetries... - The skeletal muscle Ca2+ release channel has an oxidoreductase-like domainMatthew L Baker
Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
Proc Natl Acad Sci U S A 99:12155-60. 2002..Consistent with the prediction of an oxidoreductase domain, RyR1 binds [3H]NAD+, supporting a model in which RyR1 has a oxidoreductase-like domain that could function as a type of redox sensor... - Coat protein fold and maturation transition of bacteriophage P22 seen at subnanometer resolutionsWen Jiang
National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
Nat Struct Biol 10:131-5. 2003..The cryo-EM shows that a large conformational change of the P22 capsid during maturation transition involves not only the domain movement of individual subunits, but also refolding of the capsid protein... - Architecture of the herpes simplex virus major capsid protein derived from structural bioinformaticsMatthew L Baker
National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, TX 77030, USA
J Mol Biol 331:447-56. 2003..The combined structural and sequence-based informatics has led to an architectural model of VP5. This model placed in the context of the capsid provides insights into the strategies used to achieve viral capsid stability... - Applications of a bilateral denoising filter in biological electron microscopyWen Jiang
Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
J Struct Biol 144:114-22. 2003..In as much, we have applied this technique to individual micrographs, entire 3D reconstructions, segmented proteins, and tomographic reconstructions... - A 9 angstroms single particle reconstruction from CCD captured images on a 200 kV electron cryomicroscopeChristopher R Booth
Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, TX 77030, USA
J Struct Biol 147:116-27. 2004.... - Electron cryomicroscopy of biological machines at subnanometer resolutionWah Chiu
National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
Structure 13:363-72. 2005..The consequences of these analyses can not only identify accurately some of the secondary structure elements of the molecular components in machines but also suggest structural mechanisms related to their biological functions... - Electron cryomicroscopy of single particles at subnanometer resolutionWen Jiang
National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
Curr Opin Struct Biol 15:571-7. 2005..There is now hope that, in the next few years, this technique will achieve resolutions approaching 4 A, permitting a complete trace of the protein backbone without reference to a crystal structure... - Common ancestry of herpesviruses and tailed DNA bacteriophagesMatthew L Baker
National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
J Virol 79:14967-70. 2005..On the basis of this and other emerging relationships, it seems increasingly likely that the very diverse collection of extant viruses may have arisen from a relatively small number of primordial progenitors... - Cryo-EM asymmetric reconstruction of bacteriophage P22 reveals organization of its DNA packaging and infecting machineryJuan Chang
Graduate Program in Structural and Computational Biology and Molecular Biophysics, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
Structure 14:1073-82. 2006..The coaxially packed DNA genome is encapsidated by the icosahedral shell. This complete structure unifies various biochemical, genetic, and crystallographic data of its components from the past several decades... - Ab initio modeling of the herpesvirus VP26 core domain assessed by CryoEM densityMatthew L Baker
National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, United States of America
PLoS Comput Biol 2:e146. 2006..While this new model provides for a better understanding of the assembly and interactions of VP26 in HSV-1, the approach itself may have broader applications in modeling the components of large macromolecular assemblies... - Deriving folds of macromolecular complexes through electron cryomicroscopy and bioinformatics approachesWah Chiu
Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, Texas 77030, USA
Curr Opin Struct Biol 12:263-9. 2002..Such approaches have been demonstrated with the 6.8 A structure of the rice dwarf virus to derive models for the major capsid shell proteins...
Research Grants
- Structural basis of bacteriophage T7 assembly and DNA packagingWen Jiang; Fiscal Year: 2007..The T7 structures will form the foundation for understanding the basic processes of assembly, maturation and genome packaging of not only the tailed dsDNA phages, but also the related human herpes viruses. ..
- Structural basis of bacteriophage T7 assembly and DNA packagingWen Jiang; Fiscal Year: 2010..The T7 structures will form the foundation for understanding the basic processes of assembly, maturation and genome packaging of not only the tailed dsDNA phages, but also the related human herpes viruses. ..