Wen Jiang

Summary

Affiliation: Purdue University
Country: USA

Publications

  1. pmc Cryo-EM structure of a novel calicivirus, Tulane virus
    Guimei Yu
    Markey Center for Structural Biology, Department of Biological Science, Purdue University, West Lafayette, Indiana, United States of America
    PLoS ONE 8:e59817. 2013
  2. pmc A graph theory method for determination of cryo-EM image focuses
    Wen Jiang
    Markey Center for Structural Biology, Department of Biological Sciences, Purdue University, 249 S Martin Jischke Drive, West Lafayette, IN 47906, USA
    J Struct Biol 180:343-51. 2012
  3. ncbi request reprint Backbone structure of the infectious epsilon15 virus capsid revealed by electron cryomicroscopy
    Wen Jiang
    Markey Center for Structural Biology, Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    Nature 451:1130-4. 2008
  4. ncbi request reprint Exterior modification of a DNA tetrahedron
    Chuan Zhang
    Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA
    Chem Commun (Camb) 46:6792-4. 2010
  5. pmc Conformational flexibility facilitates self-assembly of complex DNA nanostructures
    Chuan Zhang
    Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA
    Proc Natl Acad Sci U S A 105:10665-9. 2008
  6. pmc Visualization of uncorrelated, tandem symmetry mismatches in the internal genome packaging apparatus of bacteriophage T7
    Fei Guo
    Markey Center for Structural Biology, Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
    Proc Natl Acad Sci U S A 110:6811-6. 2013
  7. doi request reprint DNA-directed three-dimensional protein organization
    Chuan Zhang
    Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA
    Angew Chem Int Ed Engl 51:3382-5. 2012
  8. ncbi request reprint Controlling the chirality of DNA nanocages
    Chuan Zhang
    Department of Chemistry, Purdue University, West Lafayette, IN 47907 USA
    Angew Chem Int Ed Engl 51:7999-8002. 2012
  9. pmc Synergistic self-assembly of RNA and DNA molecules
    Seung Hyeon Ko
    Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA
    Nat Chem 2:1050-5. 2010
  10. doi request reprint Symmetry controls the face geometry of DNA polyhedra
    Chuan Zhang
    Department of Chemistry, Markey Center for Structural Biology and Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Am Chem Soc 131:1413-5. 2009

Collaborators

Detail Information

Publications33

  1. pmc Cryo-EM structure of a novel calicivirus, Tulane virus
    Guimei Yu
    Markey Center for Structural Biology, Department of Biological Science, Purdue University, West Lafayette, Indiana, United States of America
    PLoS ONE 8:e59817. 2013
    ..Residues in the S-P1 hinge between the S and P domain may play a critical role in the flexibility of P domains of C/C dimers...
  2. pmc A graph theory method for determination of cryo-EM image focuses
    Wen Jiang
    Markey Center for Structural Biology, Department of Biological Sciences, Purdue University, 249 S Martin Jischke Drive, West Lafayette, IN 47906, USA
    J Struct Biol 180:343-51. 2012
    ..The new integrated focus determination method will improve reliability of automated focus determination for large-scale data processing that is increasingly common in the cryo-EM field...
  3. ncbi request reprint Backbone structure of the infectious epsilon15 virus capsid revealed by electron cryomicroscopy
    Wen Jiang
    Markey Center for Structural Biology, Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    Nature 451:1130-4. 2008
    ..Beyond epsilon15, this method potentially offers a new approach for modelling the backbone conformations of the protein subunits in other macromolecular assemblies at near-native solution states...
  4. ncbi request reprint Exterior modification of a DNA tetrahedron
    Chuan Zhang
    Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA
    Chem Commun (Camb) 46:6792-4. 2010
    ..This paper reports an introduction of extra structural features into self-assembled DNA polyhedra...
  5. pmc Conformational flexibility facilitates self-assembly of complex DNA nanostructures
    Chuan Zhang
    Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA
    Proc Natl Acad Sci U S A 105:10665-9. 2008
    ..These results demonstrate that conformational flexibility could be explored to generate complex DNA nanostructures. The basic concept might be further extended to other biomacromolecular systems, such as RNA and proteins...
  6. pmc Visualization of uncorrelated, tandem symmetry mismatches in the internal genome packaging apparatus of bacteriophage T7
    Fei Guo
    Markey Center for Structural Biology, Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
    Proc Natl Acad Sci U S A 110:6811-6. 2013
    ..We also found a small tilt of the core stack relative to the icosahedral fivefold axis and propose that this tilt assists DNA spooling without tangling during packaging...
  7. doi request reprint DNA-directed three-dimensional protein organization
    Chuan Zhang
    Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA
    Angew Chem Int Ed Engl 51:3382-5. 2012
    ..This strategy was also applied to different 3D DNA nanostructures and different proteins...
  8. ncbi request reprint Controlling the chirality of DNA nanocages
    Chuan Zhang
    Department of Chemistry, Purdue University, West Lafayette, IN 47907 USA
    Angew Chem Int Ed Engl 51:7999-8002. 2012
    ..The resulting DNA nanocages have been characterized with a variety of methods. Such chiral control could be useful for tuning the photonic/optical properties of DNA-templated nanostructures...
  9. pmc Synergistic self-assembly of RNA and DNA molecules
    Seung Hyeon Ko
    Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA
    Nat Chem 2:1050-5. 2010
    ..The current strategy could enable the integration of the precise programmability of DNA with the rich functionality of RNA...
  10. doi request reprint Symmetry controls the face geometry of DNA polyhedra
    Chuan Zhang
    Department of Chemistry, Markey Center for Structural Biology and Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Am Chem Soc 131:1413-5. 2009
    ..As a demonstration, DNA cubes, whose each face contains four tiles, have been assembled through these approaches...
  11. ncbi request reprint Construction of RNA nanocages by re-engineering the packaging RNA of Phi29 bacteriophage
    Chenhui Hao
    1 Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA 2
    Nat Commun 5:3890. 2014
    ..The resulting RNA nanostructures have been thoroughly characterized by gel electrophoresis, cryogenic electron microscopy imaging and dynamic light scattering. ..
  12. ncbi request reprint DNA Nanocages Swallow Gold Nanoparticles (AuNPs) to Form AuNP@DNA Cage Core-Shell Structures
    Chuan Zhang
    Department of Chemistry and Markey Center for Structural Biology and Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, United States
    ACS Nano 8:1130-5. 2014
    ..The AuNP guest can be further controllably released from the host (DNA nanocages), pointing to potential applications in surface engineering of inorganic NPs and cargo delivery of DNA nanocages. ..
  13. doi request reprint Reversibly switching the surface porosity of a DNA tetrahedron
    Chuan Zhang
    Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA
    J Am Chem Soc 134:11998-2001. 2012
    ..This work may lead to the design and construction of stimuli-responsive nanocages that might find applications as smart materials...
  14. pmc Visualization of bacteriophage T3 capsids with DNA incompletely packaged in vivo
    Ping an Fang
    Markey Center for Structural Biology, Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
    J Mol Biol 384:1384-99. 2008
    ....
  15. ncbi request reprint Directed self-assembly of DNA tiles into complex nanocages
    Cheng Tian
    Department of Chemistry, Purdue University, West Lafayette, IN 47907 USA
    Angew Chem Int Ed Engl 53:8041-4. 2014
    ..This strategy greatly expands the scope of accessible DNA nanostructures and would facilitate technological applications such as nanoguest encapsulation, drug delivery, and nanoparticle organization. ..
  16. pmc Single-step antibody-based affinity cryo-electron microscopy for imaging and structural analysis of macromolecular assemblies
    Guimei Yu
    Markey Center for Structural Biology, Department of Biological Science, Purdue University, West Lafayette, IN, USA
    J Struct Biol 187:1-9. 2014
    ....
  17. pmc Obstruction of dengue virus maturation by Fab fragments of the 2H2 antibody
    Zhiqing Wang
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA
    J Virol 87:8909-15. 2013
    ....
  18. pmc Single particle cryo-electron microscopy and 3-D reconstruction of viruses
    Fei Guo
    Department of Biological Sciences, Markey Center for Structural Biology, Purdue University, West Lafayette, IN, USA
    Methods Mol Biol 1117:401-43. 2014
    ..Procedures for rigorous validation of the reconstructions and resolution evaluations using truly independent de novo initial models and refinements are also introduced. ..
  19. doi request reprint Hierarchical self-assembly of DNA into symmetric supramolecular polyhedra
    Yu He
    Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA
    Nature 452:198-201. 2008
    ..We expect that our assembly strategy can be adapted to allow the fabrication of a range of relatively complex three-dimensional structures...
  20. ncbi request reprint Cryoelectron microscopy of icosahedral virus particles
    Wen Jiang
    Department of Biological Sciences, Purdue Univesity, IN, USA
    Methods Mol Biol 369:345-63. 2007
    ..In addition, we will describe how to extract useful structural information from the three-dimensional maps...
  21. pmc Genome sequence, structural proteins, and capsid organization of the cyanophage Syn5: a "horned" bacteriophage of marine synechococcus
    Welkin H Pope
    Department of Biology, Massachusetts Institute of Technology, Cambridge MA 02139, USA
    J Mol Biol 368:966-81. 2007
    ..This phage/host system should allow detailed investigation of the physiology and biochemistry of phage propagation in marine photosynthetic bacteria...
  22. pmc Structure of epsilon15 bacteriophage reveals genome organization and DNA packaging/injection apparatus
    Wen Jiang
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 439:612-6. 2006
    ..The image reconstruction approach should be applicable to studying other biological nanomachines with components of mixed symmetries...
  23. pmc The skeletal muscle Ca2+ release channel has an oxidoreductase-like domain
    Matthew L Baker
    Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 99:12155-60. 2002
    ..Consistent with the prediction of an oxidoreductase domain, RyR1 binds [3H]NAD+, supporting a model in which RyR1 has a oxidoreductase-like domain that could function as a type of redox sensor...
  24. pmc Ab initio modeling of the herpesvirus VP26 core domain assessed by CryoEM density
    Matthew L Baker
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS Comput Biol 2:e146. 2006
    ..While this new model provides for a better understanding of the assembly and interactions of VP26 in HSV-1, the approach itself may have broader applications in modeling the components of large macromolecular assemblies...
  25. ncbi request reprint Coat protein fold and maturation transition of bacteriophage P22 seen at subnanometer resolutions
    Wen Jiang
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Nat Struct Biol 10:131-5. 2003
    ..The cryo-EM shows that a large conformational change of the P22 capsid during maturation transition involves not only the domain movement of individual subunits, but also refolding of the capsid protein...
  26. ncbi request reprint Architecture of the herpes simplex virus major capsid protein derived from structural bioinformatics
    Matthew L Baker
    National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, TX 77030, USA
    J Mol Biol 331:447-56. 2003
    ..The combined structural and sequence-based informatics has led to an architectural model of VP5. This model placed in the context of the capsid provides insights into the strategies used to achieve viral capsid stability...
  27. ncbi request reprint Applications of a bilateral denoising filter in biological electron microscopy
    Wen Jiang
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    J Struct Biol 144:114-22. 2003
    ..In as much, we have applied this technique to individual micrographs, entire 3D reconstructions, segmented proteins, and tomographic reconstructions...
  28. ncbi request reprint A 9 angstroms single particle reconstruction from CCD captured images on a 200 kV electron cryomicroscope
    Christopher R Booth
    Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, TX 77030, USA
    J Struct Biol 147:116-27. 2004
    ....
  29. ncbi request reprint Electron cryomicroscopy of biological machines at subnanometer resolution
    Wah Chiu
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Structure 13:363-72. 2005
    ..The consequences of these analyses can not only identify accurately some of the secondary structure elements of the molecular components in machines but also suggest structural mechanisms related to their biological functions...
  30. ncbi request reprint Electron cryomicroscopy of single particles at subnanometer resolution
    Wen Jiang
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Curr Opin Struct Biol 15:571-7. 2005
    ..There is now hope that, in the next few years, this technique will achieve resolutions approaching 4 A, permitting a complete trace of the protein backbone without reference to a crystal structure...
  31. pmc Common ancestry of herpesviruses and tailed DNA bacteriophages
    Matthew L Baker
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Virol 79:14967-70. 2005
    ..On the basis of this and other emerging relationships, it seems increasingly likely that the very diverse collection of extant viruses may have arisen from a relatively small number of primordial progenitors...
  32. ncbi request reprint Cryo-EM asymmetric reconstruction of bacteriophage P22 reveals organization of its DNA packaging and infecting machinery
    Juan Chang
    Graduate Program in Structural and Computational Biology and Molecular Biophysics, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Structure 14:1073-82. 2006
    ..The coaxially packed DNA genome is encapsidated by the icosahedral shell. This complete structure unifies various biochemical, genetic, and crystallographic data of its components from the past several decades...
  33. ncbi request reprint Deriving folds of macromolecular complexes through electron cryomicroscopy and bioinformatics approaches
    Wah Chiu
    Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, Texas 77030, USA
    Curr Opin Struct Biol 12:263-9. 2002
    ..Such approaches have been demonstrated with the 6.8 A structure of the rice dwarf virus to derive models for the major capsid shell proteins...

Research Grants4

  1. Structural basis of bacteriophage T7 assembly and DNA packaging
    Wen Jiang; Fiscal Year: 2007
    ..The T7 structures will form the foundation for understanding the basic processes of assembly, maturation and genome packaging of not only the tailed dsDNA phages, but also the related human herpes viruses. ..
  2. Structural basis of bacteriophage T7 assembly and DNA packaging
    Wen Jiang; Fiscal Year: 2009
    ..The T7 structures will form the foundation for understanding the basic processes of assembly, maturation and genome packaging of not only the tailed dsDNA phages, but also the related human herpes viruses. ..
  3. Structural basis of bacteriophage T7 assembly and DNA packaging
    Wen Jiang; Fiscal Year: 2010
    ..The T7 structures will form the foundation for understanding the basic processes of assembly, maturation and genome packaging of not only the tailed dsDNA phages, but also the related human herpes viruses. ..