Pei Xuan Guo

Summary

Affiliation: Purdue University
Country: USA

Publications

  1. pmc Engineering RNA for targeted siRNA delivery and medical application
    Peixuan Guo
    Department of Biomedical Engineering College of Engineering College of Medicine, University of Cincinnati, Cincinnati, OH 45221, USA
    Adv Drug Deliv Rev 62:650-66. 2010
  2. pmc Translocation of double-stranded DNA through membrane-adapted phi29 motor protein nanopores
    David Wendell
    Department of Biomedical Engineering, College of Engineering and College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USA
    Nat Nanotechnol 4:765-72. 2009
  3. pmc Controlling bacteriophage phi29 DNA-packaging motor by addition or discharge of a peptide at N-terminus of connector protein that interacts with pRNA
    Jianhe Sun
    Department of Pathobiology, Purdue Cancer Center and Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
    Nucleic Acids Res 34:5482-90. 2006
  4. pmc Binding of pRNA to the N-terminal 14 amino acids of connector protein of bacteriophage phi29
    Feng Xiao
    Department of Pathobiology, Wildon School of Bioengineering and Purdue Cancer Research Center, Purdue University, West Lafayette, IN 47907, USA
    Nucleic Acids Res 33:2640-9. 2005
  5. pmc Sequential action of ATPase, ATP, ADP, Pi and dsDNA in procapsid-free system to enlighten mechanism in viral dsDNA packaging
    Chad Schwartz
    The School of Environmental, Energy, Biological, and Medical Engineering SEEBME, Nanobiomedical Center, University of Cincinnati, Cincinnati, OH 45267, USA
    Nucleic Acids Res 40:2577-86. 2012
  6. pmc One-way traffic of a viral motor channel for double-stranded DNA translocation
    Peng Jing
    Nanobiomedical Center, College of Engineering and College of Medicine, University of Cincinnati, Cincinnati, Ohio 45267, USA
    Nano Lett 10:3620-7. 2010
  7. pmc Uniqueness, advantages, challenges, solutions, and perspectives in therapeutics applying RNA nanotechnology
    Peixuan Guo
    Nanobiotechnology Center, Markey Cancer Center and Department of Pharmaceutical Sciences, University of Kentucky, Lexington, Kentucky 40536, USA
    Nucleic Acid Ther 22:226-45. 2012
  8. pmc Viral nanomotors for packaging of dsDNA and dsRNA
    Peixuan Guo
    Department of Comparative Pathobiology and Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
    Mol Microbiol 64:886-903. 2007
  9. ncbi request reprint Quantum dots for robust and simple assays using single particles in nanodevices
    Peixuan Guo
    Purdue University, West hafayette, Indiada John Hopkins University, USA
    Nanomedicine 1:122-4. 2005
  10. ncbi request reprint Methods for structural and functional analysis of an RNA hexamer of bacterial virus phi29 DNA packaging motor
    Pei Xuan Guo
    Department of Pathobiology and Purdue Cancer Center, Purdue University, West Lafayette, IN 47907, USA
    Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 34:533-43. 2002

Collaborators

  • H Zhang
  • Ying Cai
  • Chiming Wei
  • Chengde Mao
  • Faqing Huang
  • Feng Li
  • Feng Xiao
  • Dan Shu
  • Tae Jin Lee
  • Songchuan Guo
  • Wulf Dieter Moll
  • Lisa P Huang
  • Chad Schwartz
  • Peng Jing
  • Borries Demeler
  • Carlo Montemagno
  • David Wendell
  • R Holland Cheng
  • Joseph Che Yen Wang
  • Stephen Hoeprich
  • Lisa Huang
  • Seung Hyeon Ko
  • Alexander J DiMauro
  • Jianhe Sun
  • Yin Yin Guo
  • Annette Khaled
  • Huaming Fang
  • Farzin Haque
  • Jinchuan Sun
  • Peter Schoen
  • Oana Coban
  • Cagri Savran
  • Jia Geng
  • Dominik Green
  • Varuni Subramaniam
  • Chun Li Chang
  • Dan Liang
  • Yi Chen
  • Jiashun Jin
  • John J Tanner
  • Dawei Lin
  • Dale B Karr
  • Forrest Blocker
  • Sulma Mohammed
  • Nuska Tschammer
  • Martha N Simon

Detail Information

Publications40

  1. pmc Engineering RNA for targeted siRNA delivery and medical application
    Peixuan Guo
    Department of Biomedical Engineering College of Engineering College of Medicine, University of Cincinnati, Cincinnati, OH 45221, USA
    Adv Drug Deliv Rev 62:650-66. 2010
    ....
  2. pmc Translocation of double-stranded DNA through membrane-adapted phi29 motor protein nanopores
    David Wendell
    Department of Biomedical Engineering, College of Engineering and College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USA
    Nat Nanotechnol 4:765-72. 2009
    ..8 nS in 1 M KCl. This engineered and membrane-adapted phage connector is expected to have applications in microelectromechanical sensing, microreactors, gene delivery, drug loading and DNA sequencing...
  3. pmc Controlling bacteriophage phi29 DNA-packaging motor by addition or discharge of a peptide at N-terminus of connector protein that interacts with pRNA
    Jianhe Sun
    Department of Pathobiology, Purdue Cancer Center and Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
    Nucleic Acids Res 34:5482-90. 2006
    ....
  4. pmc Binding of pRNA to the N-terminal 14 amino acids of connector protein of bacteriophage phi29
    Feng Xiao
    Department of Pathobiology, Wildon School of Bioengineering and Purdue Cancer Research Center, Purdue University, West Lafayette, IN 47907, USA
    Nucleic Acids Res 33:2640-9. 2005
    ..It is therefore concluded that pRNA bound to the 12-fold symmetrical connector to form a pRNA-connector complex and that the foothold for pRNA is the connector but not the capsid protein...
  5. pmc Sequential action of ATPase, ATP, ADP, Pi and dsDNA in procapsid-free system to enlighten mechanism in viral dsDNA packaging
    Chad Schwartz
    The School of Environmental, Energy, Biological, and Medical Engineering SEEBME, Nanobiomedical Center, University of Cincinnati, Cincinnati, OH 45267, USA
    Nucleic Acids Res 40:2577-86. 2012
    ..Combination of the two distinct roles of gp16 and connector renews the perception of previous dsDNA packaging energy calculations and provides insight into the discrepancy between 2 and 2.5 bp per ATP...
  6. pmc One-way traffic of a viral motor channel for double-stranded DNA translocation
    Peng Jing
    Nanobiomedical Center, College of Engineering and College of Medicine, University of Cincinnati, Cincinnati, Ohio 45267, USA
    Nano Lett 10:3620-7. 2010
    ....
  7. pmc Uniqueness, advantages, challenges, solutions, and perspectives in therapeutics applying RNA nanotechnology
    Peixuan Guo
    Nanobiotechnology Center, Markey Cancer Center and Department of Pharmaceutical Sciences, University of Kentucky, Lexington, Kentucky 40536, USA
    Nucleic Acid Ther 22:226-45. 2012
    ....
  8. pmc Viral nanomotors for packaging of dsDNA and dsRNA
    Peixuan Guo
    Department of Comparative Pathobiology and Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
    Mol Microbiol 64:886-903. 2007
    ....
  9. ncbi request reprint Quantum dots for robust and simple assays using single particles in nanodevices
    Peixuan Guo
    Purdue University, West hafayette, Indiada John Hopkins University, USA
    Nanomedicine 1:122-4. 2005
  10. ncbi request reprint Methods for structural and functional analysis of an RNA hexamer of bacterial virus phi29 DNA packaging motor
    Pei Xuan Guo
    Department of Pathobiology and Purdue Cancer Center, Purdue University, West Lafayette, IN 47907, USA
    Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 34:533-43. 2002
    ..This article reviews the structure of pRNA to demonstrate that its structure plays a vital role in its function, and focuses on methods and unique approaches that lead to the elucidation of pRNA structure...
  11. ncbi request reprint A simple mathematical formula for stoichiometry quantification of viral and nanobiological assemblage using slopes of log/log plot curves
    Dan Shu
    Department of Pathobiology and Purdue Cancer Research Center, B 36 Hansen Life Science Research Building, Purdue University, West Lafayette, IN 47907, USA
    J Virol Methods 115:19-30. 2004
    ....
  12. pmc Strand and nucleotide-dependent ATPase activity of gp16 of bacterial virus phi29 DNA packaging motor
    Tae Jin Lee
    Department of Biomedical Engineering, College of Engineering and College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USA
    Virology 380:69-74. 2008
    ..The results suggest that pRNA accelerates gp16 ATPase activity more significantly than genomic dsDNA, albeit both pRNA and genomic DNA are involved in the contact with gp16 during DNA packaging...
  13. ncbi request reprint Interaction of gp16 with pRNA and DNA for genome packaging by the motor of bacterial virus phi29
    Tae Jin Lee
    Department of Pathobiology, Weldon School of Biomedical Engineering, and Cancer Center, Purdue University, West Lafayette, IN 47907, USA
    J Mol Biol 356:589-99. 2006
    ..The data also imply that, with the exception of the C18C19A20 bulge, the main role of the 5'/3' helical double-stranded region of pRNA is not for procapsid binding but for binding to gp16...
  14. pmc Assembly mechanism of the sixty-subunit nanoparticles via interaction of RNA with the reengineered protein connector of phi29 DNA-packaging motor
    Feng Xiao
    Department of Biomedical Engineering, College of Engineering College of Medicine, University of Cincinnati, Cincinnati, Ohio 45267, USA
    ACS Nano 4:3293-301. 2010
    ....
  15. pmc Specific delivery of therapeutic RNAs to cancer cells via the dimerization mechanism of phi29 motor pRNA
    Songchuan Guo
    Department of Pathobiology and Purdue Cancer Research Center, Purdue University, West Lafayette, IN 47907, USA
    Hum Gene Ther 16:1097-109. 2005
    ....
  16. ncbi request reprint Construction and 3-D computer modeling of connector arrays with tetragonal to decagonal transition induced by pRNA of phi29 DNA-packaging motor
    Yin Yin Guo
    Department of Pathobiology and Wildon School of Bioengineering, Purdue University, West Lafayette, IN 47907, USA
    J Nanosci Nanotechnol 5:856-63. 2005
    ..Both the connector array and the decagon are ideal candidates to be used as templates to build patterned suprastructures in nanotechnology...
  17. ncbi request reprint Bacterial virus phi29 DNA-packaging motor and its potential applications in gene therapy and nanotechnology
    Peixuan Guo
    Department of Pathobiology, Purdue University, West Lafayette, IN, USA
    Methods Mol Biol 300:285-324. 2005
    ....
  18. ncbi request reprint Structure and function of phi29 hexameric RNA that drives the viral DNA packaging motor: review
    Peixuan Guo
    Department of Pathobiology and Purdue Cancer Center, Purdue University, West Lafayette, Indiana 47907, USA
    Prog Nucleic Acid Res Mol Biol 72:415-72. 2002
    ..This article reviews the structure and function of this pRNA and focuses on novel methods and unique approaches that lead to the illumination of its structure and function...
  19. ncbi request reprint A viral RNA that binds ATP and contains a motif similar to an ATP-binding aptamer from SELEX
    Dan Shu
    Department of Pathobiology and Purdue Cancer Center, Purdue University, West Lafayette, Indiana 47907, USA
    J Biol Chem 278:7119-25. 2003
    ..This is the first demonstration of a natural RNA molecule that binds ATP and the first case to report the presence of a SELEX-derived RNA aptamer in living organisms...
  20. ncbi request reprint Use of PEG to acquire highly soluble DNA-packaging enzyme gp16 of bacterial virus phi29 for stoichiometry quantification
    Lisa P Huang
    Department of Pathobiology, Purdue Cancer Research Center, Purdue University, Hansen Life Science Research Building B 36, West Lafayette, IN 47907, USA
    J Virol Methods 109:235-44. 2003
    ..This result leads to a subsequent finding that the stoichiometry of gp16 for phi29 DNA-packaging was about 11+/-2. These findings will facilitate the study on this novel motor that involves three pRNA dimers and a 12-subunit connector...
  21. pmc Construction of bacteriophage phi29 DNA packaging motor and its applications in nanotechnology and therapy
    Tae Jin Lee
    Department of Biomedical Engineering, The Vontz Center for Molecular Studies, College of Engineering and College of Medicine, University of Cincinnati, Room 1301, Cincinnati, OH 45267, USA
    Ann Biomed Eng 37:2064-81. 2009
    ....
  22. pmc Reversible switching of pRNA activity on the DNA packaging motor of bacteriophage phi29
    Seung Hyeon Ko
    Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, Indiana 47907, USA
    J Am Chem Soc 130:17684-7. 2008
    ..The switching process can be repeated for multiple cycles and has been demonstrated by gel electrophoresis and a virion assembly assay...
  23. ncbi request reprint Grouping of ferritin and gold nanoparticles conjugated to pRNA of the phage phi29 DNA-packaging motor
    Wulf Dieter Moll
    Department of Comparative Pathobiology and Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
    J Nanosci Nanotechnol 7:3257-67. 2007
    ..The results provide approaches to assembled ferritin or gold-containing nano-complexes via pRNA mediated assembly...
  24. pmc RNA nanotechnology: engineering, assembly and applications in detection, gene delivery and therapy
    Peixuan Guo
    Department of Pathobiology and Purdue Cancer Center, Purdue University, West Lafayette, IN 47907, USA
    J Nanosci Nanotechnol 5:1964-82. 2005
    ..The most recent development in exploration of RNA nanoparticles for pathogen detection, drug/gene delivery, and therapeutic application is also introduced in this review...
  25. ncbi request reprint Construction of phi29 DNA-packaging RNA monomers, dimers, and trimers with variable sizes and shapes as potential parts for nanodevices
    Dan Shu
    Department of Pathobiology, Purdue Cancer Research Center, Purdue University, West Lafayette, Indiana, USA
    J Nanosci Nanotechnol 3:295-302. 2003
    ..Other conditions, including the salt requirement for the formation of monomers, dimers, and trimers, have been investigated. RNA monomers, dimers, and trimers with variable lengths are potential parts for nanodevices...
  26. ncbi request reprint Gene cloning, purification, and stoichiometry quantification of phi29 anti-receptor gp12 with potential use as special ligand for gene delivery
    Songchuan Guo
    Laboratory of Molecular Virology and Gene Therapy, Purdue University, West Lafayette, IN 47907, USA
    Gene 315:145-52. 2003
    ..The results provide a basis for future research into bacteriophage-host interaction by modifying the anti-receptor protein. The ultimate goal is to re-target the bacteriophage to new host cells for the purpose of gene delivery...
  27. pmc Controllable self-assembly of nanoparticles for specific delivery of multiple therapeutic molecules to cancer cells using RNA nanotechnology
    Annette Khaled
    Department of Pathobiology and Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907, USA
    Nano Lett 5:1797-808. 2005
    ..The use of such antigenicity-free 20-40 nm particles holds promise for the repeated long-term treatment of chronic diseases...
  28. pmc Counting of six pRNAs of phi29 DNA-packaging motor with customized single-molecule dual-view system
    Dan Shu
    Department of Comparative Pathobiology and Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
    EMBO J 26:527-37. 2007
    ..Heat-denaturation analysis confirmed that the stoichiometry of pRNA is the common multiple of 2 and 3. EM imaging of procapsid/pRNA complexes clearly revealed six ferritin particles that were conjugated to each pRNA ring...
  29. pmc Construction of a laser combiner for dual fluorescent single molecule imaging of pRNA of phi29 DNA packaging motor
    Hui Zhang
    Department of Biomedical Engineering, College of Engineering and College of Medicine, University of Cincinnati, Cincinnati, OH 45221, USA
    Biomed Microdevices 12:97-106. 2010
    ..The single molecule FRET with the dual-color imaging system was also applied to reconstructed phi29 motor pRNA monomers. Finally, techniques for dual laser alignment and tuning of laser power for dual-color excitation are discussed...
  30. ncbi request reprint Translocation of nicked but not gapped DNA by the packaging motor of bacteriophage phi29
    Wulf Dieter Moll
    Department of Pathobiology and Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
    J Mol Biol 351:100-7. 2005
    ..Thus the phi29 DNA packaging machinery tolerated nicks, but stopped at gaps. The packaging motor did not require a nick-free DNA backbone, but the presence of both DNA strands, for uninterrupted packaging...
  31. pmc Fabrication of massive sheets of single layer patterned arrays using lipid directed reengineered phi29 motor dodecamer
    Feng Xiao
    Department of Biomedical Engineering, University of Cincinnati, Cincinnati, Ohio 45221, USA
    ACS Nano 3:100-7. 2009
    ..Uniform, clean and highly ordered arrays were constructed as shown by both transmission electron microscopy and atomic force microscopy imaging...
  32. ncbi request reprint Use of acetone to attain highly active and soluble DNA packaging protein Gp16 of Phi29 for ATPase assay
    Lisa P Huang
    Department of Pathobiology, Purdue University, West Lafayette, IN 47907, USA
    Virology 312:449-57. 2003
    ..However, mutation on amino acids G(248) and D(256) did not affect the function of gp16 in DNA packaging...
  33. pmc Novel mechanism of hexamer ring assembly in protein/RNA interactions revealed by single molecule imaging
    Feng Xiao
    Department of Biomedical Engineering, College of Engineering College of Medicine, University of Cincinnati, Cincinnati, OH 45221, USA
    Nucleic Acids Res 36:6620-32. 2008
    ..This new mechanism was tested by redesigning two artificial RNAs that formed hexamer and packaged DNA. The results confirmed the stoichiometry of pRNA on the motor was the common multiple of two and three, thus, a hexamer...
  34. ncbi request reprint Only one pRNA hexamer but multiple copies of the DNA-packaging protein gp16 are needed for the motor to package bacterial virus phi29 genomic DNA
    Dan Shu
    Department of Pathobiology and Purdue Cancer Center, Purdue University, West Lafayette, IN 47907, USA
    Virology 309:108-13. 2003
    ..Only one pRNA hexamer, but multiple copies of gp16, were needed to accomplish DNA packaging. pRNA functions continuously during the entire DNA translocation process, suggesting that pRNA is a vital part of the DNA packaging motor...
  35. pmc The effect of N- or C-terminal alterations of the connector of bacteriophage phi29 DNA packaging motor on procapsid assembly, pRNA binding, and DNA packaging
    Ying Cai
    Department of Biomedical Engineering, The Vontz Center for Molecular Studies, College of Engineering and College of Medicine, University of Cincinnati, Cincinnati, Ohio 45267, USA
    Nanomedicine 4:8-18. 2008
    ....
  36. pmc Instrumentation and metrology for single RNA counting in biological complexes or nanoparticles by a single-molecule dual-view system
    Hui Zhang
    Department of Comparative Pathobiology, Purdue University, West Lafayette, Indiana 47907, USA
    RNA 13:1793-802. 2007
    ..The dual-viewing system for excitation and recording at different wavelengths simultaneously will enable the differentiation of different complexes with different labels or relative motion of each labeled component in motion machines...
  37. pmc Engineering of the fluorescent-energy-conversion arm of phi29 DNA packaging motor for single-molecule studies
    Tae Jin Lee
    Department of Biomedical Engineering, The Vontz Center for Molecular Studies, College of Engineering, University of Cincinnati, Cincinnati, OH 45267, USA
    Small 5:2453-9. 2009
    ..The activity of the re-engineered motor with eGFP-gp16 is also observed directly with a bright-field microscope via its ability to transport a 2-microm-sized cargo bound to the DNA...
  38. pmc Adjustable ellipsoid nanoparticles assembled from re-engineered connectors of the bacteriophage phi29 DNA packaging motor
    Feng Xiao
    Department of Biomedical Engineering, College of Engineering and College of Medicine, University of Cincinnati, Cincinnati, Ohio 45221, USA
    ACS Nano 3:2163-70. 2009
    ..This further extends the application of this nanoparticle to pathogen detection and disease diagnosis by signal enhancement...
  39. ncbi request reprint Crystallization of Phi29 spindle-shaped nano-bar anti-receptor with glycosidase domain
    Alexander J DiMauro
    Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
    J Nanosci Nanotechnol 7:2616-22. 2007
    ..These results suggest a function for gp12 as a cell-wall degrading enzyme in addition to its role in recognition of the host receptor...
  40. ncbi request reprint Computer modeling of three-dimensional structure of DNA-packaging RNA (pRNA) monomer, dimer, and hexamer of Phi29 DNA packaging motor
    Stephen Hoeprich
    Department of Pathobiology, Purdue University, West Lafayette, Indiana 47907, USA
    J Biol Chem 277:20794-803. 2002
    ..pRNA hexamer docking with the connector crystal structure reveals a very impressive match with the available biochemical, genetic, and physical data...