Research Topics
| Pei Xuan GuoSummaryAffiliation: Purdue University Country: USA Publications
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Detail Information
Publications
Engineering RNA for targeted siRNA delivery and medical applicationPeixuan Guo
Department of Biomedical Engineering College of Engineering College of Medicine, University of Cincinnati, Cincinnati, OH 45221, USA
Adv Drug Deliv Rev 62:650-66. 2010....- Uniqueness, advantages, challenges, solutions, and perspectives in therapeutics applying RNA nanotechnologyPeixuan Guo
Nanobiotechnology Center, Markey Cancer Center and Department of Pharmaceutical Sciences, University of Kentucky, Lexington, Kentucky 40536, USA
Nucleic Acid Ther 22:226-45. 2012.... 
Methods for structural and functional analysis of an RNA hexamer of bacterial virus phi29 DNA packaging motorPei Xuan Guo
Department of Pathobiology and Purdue Cancer Center, Purdue University, West Lafayette, IN 47907, USA
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 34:533-43. 2002..This article reviews the structure of pRNA to demonstrate that its structure plays a vital role in its function, and focuses on methods and unique approaches that lead to the elucidation of pRNA structure...- Quantum dots for robust and simple assays using single particles in nanodevicesPeixuan Guo
Purdue University, West hafayette, Indiada John Hopkins University, USA
Nanomedicine 1:122-4. 2005 - Viral nanomotors for packaging of dsDNA and dsRNAPeixuan Guo
Department of Comparative Pathobiology and Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
Mol Microbiol 64:886-903. 2007.... - A simple mathematical formula for stoichiometry quantification of viral and nanobiological assemblage using slopes of log/log plot curvesDan Shu
Department of Pathobiology and Purdue Cancer Research Center, B-36 Hansen Life Science Research Building, Purdue University, West Lafayette, IN 47907, USA
J Virol Methods 115:19-30. 2004.... - Specific delivery of therapeutic RNAs to cancer cells via the dimerization mechanism of phi29 motor pRNASongchuan Guo
Department of Pathobiology and Purdue Cancer Research Center, Purdue University, West Lafayette, IN 47907, USA
Hum Gene Ther 16:1097-109. 2005.... - Construction and 3-D computer modeling of connector arrays with tetragonal to decagonal transition induced by pRNA of phi29 DNA-packaging motorYin Yin Guo
Department of Pathobiology and Wildon School of Bioengineering, Purdue University, West Lafayette, IN 47907, USA
J Nanosci Nanotechnol 5:856-63. 2005..Both the connector array and the decagon are ideal candidates to be used as templates to build patterned suprastructures in nanotechnology... - Bacterial virus phi29 DNA-packaging motor and its potential applications in gene therapy and nanotechnologyPeixuan Guo
Department of Pathobiology, Purdue University, West Lafayette, IN, USA
Methods Mol Biol 300:285-324. 2005.... - Interaction of gp16 with pRNA and DNA for genome packaging by the motor of bacterial virus phi29Tae-Jin Lee
Department of Pathobiology, Weldon School of Biomedical Engineering, and Cancer Center, Purdue University, West Lafayette, IN 47907, USA
J Mol Biol 356:589-99. 2006..The data also imply that, with the exception of the C18C19A20 bulge, the main role of the 5'/3' helical double-stranded region of pRNA is not for procapsid binding but for binding to gp16... - Strand and nucleotide-dependent ATPase activity of gp16 of bacterial virus phi29 DNA packaging motorTae Jin Lee
Department of Biomedical Engineering, College of Engineering and College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USA
Virology 380:69-74. 2008..The results suggest that pRNA accelerates gp16 ATPase activity more significantly than genomic dsDNA, albeit both pRNA and genomic DNA are involved in the contact with gp16 during DNA packaging... - Assembly mechanism of the sixty-subunit nanoparticles via interaction of RNA with the reengineered protein connector of phi29 DNA-packaging motorFeng Xiao
Department of Biomedical Engineering, College of Engineering College of Medicine, University of Cincinnati, Cincinnati, Ohio 45267, USA
ACS Nano 4:3293-301. 2010.... - Reversible switching of pRNA activity on the DNA packaging motor of bacteriophage phi29Seung Hyeon Ko
Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, Indiana 47907, USA
J Am Chem Soc 130:17684-7. 2008..The switching process can be repeated for multiple cycles and has been demonstrated by gel electrophoresis and a virion assembly assay... - Construction of bacteriophage phi29 DNA packaging motor and its applications in nanotechnology and therapyTae Jin Lee
Department of Biomedical Engineering, The Vontz Center for Molecular Studies, College of Engineering and College of Medicine, University of Cincinnati, Room 1301, Cincinnati, OH 45267, USA
Ann Biomed Eng 37:2064-81. 2009.... - Translocation of double-stranded DNA through membrane-adapted phi29 motor protein nanoporesDavid Wendell
Department of Biomedical Engineering, College of Engineering and College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USA
Nat Nanotechnol 4:765-72. 2009..8 nS in 1 M KCl. This engineered and membrane-adapted phage connector is expected to have applications in microelectromechanical sensing, microreactors, gene delivery, drug loading and DNA sequencing... - Counting of six pRNAs of phi29 DNA-packaging motor with customized single-molecule dual-view systemDan Shu
Department of Comparative Pathobiology and Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
EMBO J 26:527-37. 2007..Heat-denaturation analysis confirmed that the stoichiometry of pRNA is the common multiple of 2 and 3. EM imaging of procapsid/pRNA complexes clearly revealed six ferritin particles that were conjugated to each pRNA ring... - Grouping of ferritin and gold nanoparticles conjugated to pRNA of the phage phi29 DNA-packaging motorWulf Dieter Moll
Department of Comparative Pathobiology and Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
J Nanosci Nanotechnol 7:3257-67. 2007..The results provide approaches to assembled ferritin or gold-containing nano-complexes via pRNA mediated assembly... - RNA nanotechnology: engineering, assembly and applications in detection, gene delivery and therapyPeixuan Guo
Department of Pathobiology and Purdue Cancer Center, Purdue University, West Lafayette, IN 47907, USA
J Nanosci Nanotechnol 5:1964-82. 2005..The most recent development in exploration of RNA nanoparticles for pathogen detection, drug/gene delivery, and therapeutic application is also introduced in this review... - Controllable self-assembly of nanoparticles for specific delivery of multiple therapeutic molecules to cancer cells using RNA nanotechnologyAnnette Khaled
Department of Pathobiology and Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907, USA
Nano Lett 5:1797-808. 2005..The use of such antigenicity-free 20-40 nm particles holds promise for the repeated long-term treatment of chronic diseases... - Structure and function of phi29 hexameric RNA that drives the viral DNA packaging motor: reviewPeixuan Guo
Department of Pathobiology and Purdue Cancer Center, Purdue University, West Lafayette, Indiana 47907, USA
Prog Nucleic Acid Res Mol Biol 72:415-72. 2002..This article reviews the structure and function of this pRNA and focuses on novel methods and unique approaches that lead to the illumination of its structure and function... - A viral RNA that binds ATP and contains a motif similar to an ATP-binding aptamer from SELEXDan Shu
Department of Pathobiology and Purdue Cancer Center, Purdue University, West Lafayette, Indiana 47907, USA
J Biol Chem 278:7119-25. 2003..This is the first demonstration of a natural RNA molecule that binds ATP and the first case to report the presence of a SELEX-derived RNA aptamer in living organisms... - Use of PEG to acquire highly soluble DNA-packaging enzyme gp16 of bacterial virus phi29 for stoichiometry quantificationLisa P Huang
Department of Pathobiology, Purdue Cancer Research Center, Purdue University, Hansen Life Science Research Building B-36, West Lafayette, IN 47907, USA
J Virol Methods 109:235-44. 2003..This result leads to a subsequent finding that the stoichiometry of gp16 for phi29 DNA-packaging was about 11+/-2. These findings will facilitate the study on this novel motor that involves three pRNA dimers and a 12-subunit connector... - Gene cloning, purification, and stoichiometry quantification of phi29 anti-receptor gp12 with potential use as special ligand for gene deliverySongchuan Guo
Laboratory of Molecular Virology and Gene Therapy, Purdue University, West Lafayette, IN 47907, USA
Gene 315:145-52. 2003..The results provide a basis for future research into bacteriophage-host interaction by modifying the anti-receptor protein. The ultimate goal is to re-target the bacteriophage to new host cells for the purpose of gene delivery... - Construction of phi29 DNA-packaging RNA monomers, dimers, and trimers with variable sizes and shapes as potential parts for nanodevicesDan Shu
Department of Pathobiology, Purdue Cancer Research Center, Purdue University, West Lafayette, Indiana, USA
J Nanosci Nanotechnol 3:295-302. 2003..Other conditions, including the salt requirement for the formation of monomers, dimers, and trimers, have been investigated. RNA monomers, dimers, and trimers with variable lengths are potential parts for nanodevices... - Controlling bacteriophage phi29 DNA-packaging motor by addition or discharge of a peptide at N-terminus of connector protein that interacts with pRNAJianhe Sun
Department of Pathobiology, Purdue Cancer Center and Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
Nucleic Acids Res 34:5482-90. 2006.... - Only one pRNA hexamer but multiple copies of the DNA-packaging protein gp16 are needed for the motor to package bacterial virus phi29 genomic DNADan Shu
Department of Pathobiology and Purdue Cancer Center, Purdue University, West Lafayette, IN 47907, USA
Virology 309:108-13. 2003..Only one pRNA hexamer, but multiple copies of gp16, were needed to accomplish DNA packaging. pRNA functions continuously during the entire DNA translocation process, suggesting that pRNA is a vital part of the DNA packaging motor... - Construction of a laser combiner for dual fluorescent single molecule imaging of pRNA of phi29 DNA packaging motorHui Zhang
Department of Biomedical Engineering, College of Engineering and College of Medicine, University of Cincinnati, Cincinnati, OH 45221, USA
Biomed Microdevices 12:97-106. 2010..The single molecule FRET with the dual-color imaging system was also applied to reconstructed phi29 motor pRNA monomers. Finally, techniques for dual laser alignment and tuning of laser power for dual-color excitation are discussed... - Fabrication of massive sheets of single layer patterned arrays using lipid directed reengineered phi29 motor dodecamerFeng Xiao
Department of Biomedical Engineering, University of Cincinnati, Cincinnati, Ohio 45221, USA
ACS Nano 3:100-7. 2009..Uniform, clean and highly ordered arrays were constructed as shown by both transmission electron microscopy and atomic force microscopy imaging... - Use of acetone to attain highly active and soluble DNA packaging protein Gp16 of Phi29 for ATPase assayLisa P Huang
Department of Pathobiology, Purdue University, West Lafayette, IN 47907, USA
Virology 312:449-57. 2003..However, mutation on amino acids G(248) and D(256) did not affect the function of gp16 in DNA packaging... - Novel mechanism of hexamer ring assembly in protein/RNA interactions revealed by single molecule imagingFeng Xiao
Department of Biomedical Engineering, College of Engineering College of Medicine, University of Cincinnati, Cincinnati, OH 45221, USA
Nucleic Acids Res 36:6620-32. 2008..This new mechanism was tested by redesigning two artificial RNAs that formed hexamer and packaged DNA. The results confirmed the stoichiometry of pRNA on the motor was the common multiple of two and three, thus, a hexamer... - The effect of N- or C-terminal alterations of the connector of bacteriophage phi29 DNA packaging motor on procapsid assembly, pRNA binding, and DNA packagingYing Cai
Department of Biomedical Engineering, The Vontz Center for Molecular Studies, College of Engineering and College of Medicine, University of Cincinnati, Cincinnati, Ohio 45267, USA
Nanomedicine 4:8-18. 2008.... - Instrumentation and metrology for single RNA counting in biological complexes or nanoparticles by a single-molecule dual-view systemHui Zhang
Department of Comparative Pathobiology, Purdue University, West Lafayette, Indiana 47907, USA
RNA 13:1793-802. 2007..The dual-viewing system for excitation and recording at different wavelengths simultaneously will enable the differentiation of different complexes with different labels or relative motion of each labeled component in motion machines... - Binding of pRNA to the N-terminal 14 amino acids of connector protein of bacteriophage phi29Feng Xiao
Department of Pathobiology, Wildon School of Bioengineering and Purdue Cancer Research Center, Purdue University, West Lafayette, IN 47907, USA
Nucleic Acids Res 33:2640-9. 2005..It is therefore concluded that pRNA bound to the 12-fold symmetrical connector to form a pRNA-connector complex and that the foothold for pRNA is the connector but not the capsid protein... - Translocation of nicked but not gapped DNA by the packaging motor of bacteriophage phi29Wulf-Dieter Moll
Department of Pathobiology and Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
J Mol Biol 351:100-7. 2005..Thus the phi29 DNA packaging machinery tolerated nicks, but stopped at gaps. The packaging motor did not require a nick-free DNA backbone, but the presence of both DNA strands, for uninterrupted packaging... - Adjustable ellipsoid nanoparticles assembled from re-engineered connectors of the bacteriophage phi29 DNA packaging motorFeng Xiao
Department of Biomedical Engineering, College of Engineering and College of Medicine, University of Cincinnati, Cincinnati, Ohio 45221, USA
ACS Nano 3:2163-70. 2009..This further extends the application of this nanoparticle to pathogen detection and disease diagnosis by signal enhancement... - Engineering of the fluorescent-energy-conversion arm of phi29 DNA packaging motor for single-molecule studiesTae Jin Lee
Department of Biomedical Engineering, The Vontz Center for Molecular Studies, College of Engineering, University of Cincinnati, Cincinnati, OH 45267, USA
Small 5:2453-9. 2009..The activity of the re-engineered motor with eGFP-gp16 is also observed directly with a bright-field microscope via its ability to transport a 2-microm-sized cargo bound to the DNA... - Crystallization of Phi29 spindle-shaped nano-bar anti-receptor with glycosidase domainAlexander J DiMauro
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
J Nanosci Nanotechnol 7:2616-22. 2007..These results suggest a function for gp12 as a cell-wall degrading enzyme in addition to its role in recognition of the host receptor... - Computer modeling of three-dimensional structure of DNA-packaging RNA (pRNA) monomer, dimer, and hexamer of Phi29 DNA packaging motorStephen Hoeprich
Department of Pathobiology, Purdue University, West Lafayette, Indiana 47907, USA
J Biol Chem 277:20794-803. 2002..pRNA hexamer docking with the connector crystal structure reveals a very impressive match with the available biochemical, genetic, and physical data...