Research Topics
| JAMES FLEETSummaryAffiliation: Purdue University Country: USA Publications
Research Grants
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Detail Information
Publications
Case-control genome-wide association study of rheumatoid arthritis from Genetic Analysis Workshop 16 using penalized orthogonal-components regression-linear discriminant analysisMin Zhang
Department of Statistics, Purdue University, 150 North University Street, West Lafayette, IN 47907, USA
BMC Proc 3:S17. 2009..In addition to the two regions on chromosomes 6 and 9 previously associated with RA by GWAS, we identified SNPs on chromosomes 10 and 18 as potential candidates for further investigation...- 1,25 dihydroxyvitamin D-mediated orchestration of anticancer, transcript-level effects in the immortalized, non-transformed prostate epithelial cell line, RWPE1Pavlo L Kovalenko
Department of Foods and Nutrition and the Interdepartmental Nutrition Program, Purdue University, West Lafayette, IN 47907 2059, USA
BMC Genomics 11:26. 2010..Still, the molecular mechanisms and the gene targets for vitamin D-mediated prostate cancer prevention are unknown... 
Using genomics to understand intestinal biologyJ C Fleet
Department of Foods and Nutrition and Interdepartmental Nutrition Program, Purdue University, 700 West State St, West Lafayette, IN 47906 2059, USA
J Physiol Biochem 63:83-96. 2007....- What have genomic and proteomic approaches told us about vitamin D and cancer?James C Fleet
Department of Foods and Nutrition, Purdue University, 700 West State St, West Lafayette, IN 47906 2059, USA
Nutr Rev 65:S127-30. 2007 - Molecular regulation of calcium and bone metabolism through the vitamin D receptorJ C Fleet
Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907-2059, USA
J Musculoskelet Neuronal Interact 6:336-7. 2006 - Serum metabolite profiles and target tissue gene expression define the effect of cholecalciferol intake on calcium metabolism in rats and miceJames C Fleet
Department of Foods and Nutrition and the Interdepartmental Nutrition Program, Purdue University, West Lafayette, IN 47907 2059, USA
J Nutr 138:1114-20. 2008..Our data show that modeling human VD3 status requires lower intake than the current NRC rodent requirement (1000-IU/kg diet). Also, although rodents are very tolerant of high-VD3 intake, it cannot compensate for moderate Ca restriction... - Systems genetics of mineral metabolismJames C Fleet
Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47906, USA
J Nutr 141:520-5. 2011..e., ionomics. This short review will explain the value of forward genetics and ionomics as tools for exploring mammalian mineral metabolism... - Molecular actions of vitamin D contributing to cancer preventionJames C Fleet
Department of Foods and Nutrition, Purdue University, 700 West State Street, West Lafayette, IN 47906 2059, USA
Mol Aspects Med 29:388-96. 2008.... - The effect of differentiation on 1,25 dihydroxyvitamin D-mediated gene expression in the enterocyte-like cell line, Caco-2Min Cui
Department of Foods and Nutrition, Purdue University, West Lafayette, Indiana, USA
J Cell Physiol 218:113-21. 2009..This suggests that decreased recruitment of VDR to vitamin D response elements also contributes to the blunted transcriptional responsiveness to 1,25(OH)(2)D(3) in proliferating Caco-2 cells... - Calcium transporter 1 and epithelial calcium channel messenger ribonucleic acid are differentially regulated by 1,25 dihydroxyvitamin D3 in the intestine and kidney of miceYurong Song
Interdepartmental Nutrition Program, Purdue University, West Lafayette, Indiana 47907, USA
Endocrinology 144:3885-94. 2003..The rapid induction of intestinal CaT1 mRNA expression by 1,25(OH)(2)D(3), and the marked induction at weaning, suggest that CaT1 is critical for 1,25(OH)(2)D(3)-mediated intestinal Ca absorption... - Control of differentiation-induced calbindin-D9k gene expression in Caco-2 cells by cdx-2 and HNF-1alphaLiyong Wang
Interdepartmental Nutrition Program, Purdue University, West Lafayette, Indiana 47907, USA
Am J Physiol Gastrointest Liver Physiol 287:G943-53. 2004..Our data suggest that cdx-2 is a permissive factor that influences basal CaBP expression in enterocytes and that HNF-1alpha modulates CaBP gene expression during cellular differentiation... - 1,25 dihydroxycholecalciferol-mediated calcium absorption and gene expression are higher in female than in male miceYurong Song
Department of Foods and Nutrition and the Interdepartmental Nutrition Program, Purdue University, West Lafayette, IN 47907, USA
J Nutr 134:1857-61. 2004..Our data indicate that female mice are more sensitive to changes in serum 1,25(OH)(2) D levels than males and that this must be considered when using mice to study calcium and bone biology... - Vitamin D receptor (VDR) knockout mice reveal VDR-independent regulation of intestinal calcium absorption and ECaC2 and calbindin D9k mRNAYurong Song
Interdepartmental Nutrition Program, Purdue University, West Lafayette, IN 47907, USA
J Nutr 133:374-80. 2003..In addition, our data suggest that the 2% Ca diet promotes a vitamin D receptor-independent anabolic effect on bone formation and calcium absorption, leading to improved calcium balance even in the presence of high PTH levels... - Simultaneous genome-wide association studies of anti-cyclic citrullinated peptide in rheumatoid arthritis using penalized orthogonal-components regressionYanzhu Lin
Department of Statistics, Purdue University, West Lafayette, Indiana 47907, USA YL
BMC Proc 3:S20. 2009..Bioinformatic analysis of these genomic regions showed most of them harbor protein-coding gene(s)... - Effects of MAPK signaling on 1,25-dihydroxyvitamin D-mediated CYP24 gene expression in the enterocyte-like cell line, Caco-2Min Cui
Department of Foods and Nutrition, Purdue University, West Lafayette, Indiana 47906 2059, USA
J Cell Physiol 219:132-42. 2009..Our data show that there are both promoter-specific and cell stage-specific roles for the ERK signaling pathway on 1,25(OH)(2)D(3)-mediated gene induction in enterocyte-like Caco-2 cells... - Genomic and proteomic approaches for probing the role of vitamin D in healthJames C Fleet
Interdepartmental Nutrition Program and Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907 2059, USA
Am J Clin Nutr 80:1730S-4S. 2004.... - Short-term low-protein intake does not increase serum parathyroid hormone concentration in humansWayne W Campbell
Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907, USA
J Nutr 134:1900-4. 2004..These findings do not support the hypothesis that the short-term ingestion of inadequate dietary protein increases serum PTH concentration... - Gene expression profiling of Caco-2 BBe cells suggests a role for specific signaling pathways during intestinal differentiationJames C Fleet
Interdepartmental Nutrition Program, Purdue University, West Lafayette, Indiana 47907
Physiol Genomics 13:57-68. 2003.... - Vitamin D requirements: current and futureConnie M Weaver
Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907 2059, USA
Am J Clin Nutr 80:1735S-9S. 2004..So much new information on vitamin D and health has been collected since the requirements were set in 1997 that this nutrient is likely the most in need of revised requirements... - Vitamin D-inducible calcium transport and gene expression in three Caco-2 cell linesJames C Fleet
Department of Foods and Nutrition, Purdue University, West Lafayette, Indiana 47906, USA
Am J Physiol Gastrointest Liver Physiol 283:G618-25. 2002..Basal CaBP mRNA (86 times greater than P) and protein levels were highest in TC7 cells and were not associated with higher net CaTx, suggesting CaBP may not be rate limiting for CaTx in these cells... - Intestinal vitamin D receptor is required for normal calcium and bone metabolism in miceYingben Xue
Department of Foods and Nutrition, Purdue University, West Lafayette, Indiana 47906 2059, USA
Gastroenterology 136:1317-27, e1-2. 2009..To specifically study the effects of VDR in intestinal calcium absorption, we investigated whether restoration of intestinal VDR is sufficient to recover the phenotype of VDR-knockout mice... - Intestinal resistance to 1,25 dihydroxyvitamin D in mice heterozygous for the vitamin D receptor knockout alleleYurong Song
Interdepartmental Nutrition Program and Department of Foods and Nutrition, Purdue University, 700 West State Street, West Lafayette, IN 47906 2059, USA
Endocrinology 148:1396-402. 2007..Our data show that low VDR levels lead to resistance of intestinal Ca absorption to 1,25(OH)(2) D(3), and this resistance may be due to a role for the VDR (and VDR level) in the translation of CaBP... - Inadequate protein intake affects skeletal muscle transcript profiles in older humansAnna E Thalacker Mercer
Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907, USA
Am J Clin Nutr 85:1344-52. 2007..Inadequate dietary protein intake causes adverse changes in the morphology and function of skeletal muscle. These changes may be reflected in early alterations in muscle messenger RNA levels... - Sodium-dependent phosphate uptake in the jejunum is post-transcriptionally regulated in pigs fed a low-phosphorus diet and is independent of dietary calcium concentrationKari L Saddoris
Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA
J Nutr 140:731-6. 2010..These data suggest that restricting dietary aP concentration by only 43% stimulates Na(+)-dependent phosphate uptake and expression of the NaPi-IIb protein in the BBM of the small intestine and through a post-transcriptional mechanism... - Nucleo-cytoplasmic cycling of the vitamin D receptor in the enterocyte-like cell line, Caco-2Anna Klopot
Department of Foods and Nutrition and the Interdepartmental Nutrition Program, Purdue University, West Lafayette, IN 47907 2059, USA
J Cell Biochem 100:617-28. 2007..In addition, ligand-dependent VDR nuclear import appears to be balanced by export, thus accounting for the lack of nuclear VDR accumulation even when VDR import is significantly elevated... - Constitutive activation of the mitogen-activated protein kinase pathway impairs vitamin D signaling in human prostate epithelial cellsZhentao Zhang
Department of Foods and Nutrition, Purdue University, West Lafayette, Indiana 47907 2059, USA
J Cell Physiol 224:433-42. 2010..This is due in part to the phosphorylation of critical amino acids in the RXRalpha AF-1 domain and impaired co-activator recruitment... - Rapid, membrane-initiated actions of 1,25 dihydroxyvitamin D: what are they and what do they mean?James C Fleet
Department of Foods and Nutrition and the Interdepartmental Nutrition Program, Purdue University, West Lafayette, IN 47907, USA
J Nutr 134:3215-8. 2004..This review summarizes recent research on the rapid actions of 1,25(OH)(2) D and identifies questions that remain to be answered in this area... - Activation of rapid signaling pathways does not contribute to 1 alpha,25-dihydroxyvitamin D3-induced growth inhibition of mouse prostate epithelial progenitor cellsJia Li
Interdepartmental Nutrition Program, Purdue University, 700 W State Street, West Lafayette, Indiana 47907, USA
J Cell Biochem 107:1031-6. 2009..In conclusion, 1,25(OH)(2)D did not mediate rapid activation of ERK1/2 or PKC alpha in MPEC3 and therefore the growth inhibitory effect of 1,25(OH)(2)D is independent of rapid activation of these signaling pathways in this cell type... - Effects of hindlimb unloading and bisphosphonates on the serum proteome of ratsYongdong Zhao
Department of Foods and Nutrition, Purdue University, 700 W State St, West Lafayette, IN 47907 2059, USA
Bone 41:646-58. 2007..e. drug related), and 17 common to HU and HUA (e.g. potential mental stress or muscle loss markers). Spots were identified by LC-MS/MS after in-gel trypsin digestion and were found to relate to a variety of physiological functions... - Dietary vitamin D and vitamin D receptor level modulate epithelial cell proliferation and apoptosis in the prostatePavlo L Kovalenko
Center for Cancer Research, Department of Foods and Nutrition, Purdue University, West Lafayette, Indiana, USA
Cancer Prev Res (Phila) 4:1617-25. 2011..Our data show that low diet VDR or VDR deletion provide a prostate environment that is permissive to early procarcinogenic events that enhance prostate cancer risk... - Generation of a transgenic mouse for colorectal cancer research with intestinal cre expression limited to the large intestineYingben Xue
Purdue University, West Lafayette, IN 47906 2059, USA
Mol Cancer Res 8:1095-104. 2010..Our new model has improved features for colon cancer research, that is, transgene expression is limited to the epithelium of the large bowel with normal cells found next to genetically modified cells... - Forward genetics used to identify new gene Mon1a with critical role in controlling macrophage iron metabolism and iron recycling from erythrocytesRebecca A McCreedy
Department of Foods and Nutrition, and Center for Gene Environment Interactions, Purdue University, West Lafayette, Indiana 47906 2059, USA
Nutr Rev 67:607-10. 2009..The forward genetics approach, which has currently only been used sparingly by the nutrition research community, offers a powerful and unbiased approach to identifying genes important in nutritional metabolism... - High dietary vitamin D prevents hypocalcemia and osteomalacia in CYP27B1 knockout miceMatthew J Rowling
Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
J Nutr 137:2608-15. 2007..However, neither high dietary Ca nor high dietary VD3 is sufficient to fully recover the phenotype of CYP27B1 KO mice... - Effect of cellular environment on the selective activation of the vitamin D receptor by 1alpha,25-dihydroxyvitamin D3 and its analog 1alpha-fluoro-16-ene-20-epi-23-ene-26,27-bishomo-25-hydroxyvitamin D3 (Ro-26-9228)Ayesha Ismail
Department of Endocrine Neoplasia and Hormonal Disorders, Unit 435, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA
Mol Endocrinol 18:874-87. 2004..These results suggest that Ro-26-9228 has poor ability to promote the accumulation of a proteasome-sensitive, transcriptionally active VDR isoform in Caco-2 cells, whereas it does not have this limitation in hFOB cells... - 1,25-Dihydroxyvitamin D and 25-hydroxyvitamin D--mediated regulation of TRPV6 (a putative epithelial calcium channel) mRNA expression in Caco-2 cellsShveta Taparia
Mineral Bioavailability Laboratory, Jean Mayer USDA Human Nutrition, Research Center on Aging, Tufts University, 711 Washington Street, Boston (MA) 02111, USA
Eur J Nutr 45:196-204. 2006..In addition, the prohormone form of 1,25 dihydroxyvitamin D, i. e. the 25 hydroxyvitamin D, induced TRPV6 mRNA expression in Caco-2 cells... - Excentric cleavage products of beta-carotene inhibit estrogen receptor positive and negative breast tumor cell growth in vitro and inhibit activator protein-1-mediated transcriptional activationElmi C Tibaduiza
Molecular Pharmacology Research Center, New England Medical Center, Boston, MA 02111, USA
J Nutr 132:1368-75. 2002.... - Transgenic expression of the human Vitamin D receptor (hVDR) in the duodenum of VDR-null mice attenuates the age-dependent decline in calcium absorptionHilary D Marks
Department of Endocrine Neoplasia and Hormonal Disorders, Unit 435, The University of Texas, M D Anderson Cancer Center, Houston, TX 77030, USA
J Steroid Biochem Mol Biol 103:513-6. 2007..In addition, hVDR+/mVDR-null mice responded better to the late rescue diet suggesting that expression of VDR in the proximal small intestine protected the calcium absorbing machinery from age-dependent decline... - Renal cell cancer and nuclear receptor levels--biomarkers or functionally relevant?James C Fleet
J Urol 178:1144-5. 2007 - Vitamin D receptor alleles, periodontal disease progression, and tooth loss in the VA dental longitudinal studyKoji Inagaki
Department of Health Policy and Health Services Research, Boston University Goldman School of Dental Medicine, Boston, MA, USA
J Periodontol 74:161-7. 2003..The relationship between the vitamin D receptor (VDR) genotype and periodontal disease is not known. This study compared periodontal disease progression among polymorphisms of 2 VDR genes in men in the VA Dental Longitudinal Study... - Dairy consumption and the prevention of colon cancer: is there more to the story than calcium?James C Fleet
Am J Clin Nutr 83:527-8. 2006
Research Grants
- Intestinal Calcium Absorption: Molecular MechanismJames C Fleet; Fiscal Year: 2010..This work will lay the foundation for developing osteoporosis prevention strategies that maximize vitamin D action in the intestine and optimize the absorption of dietary calcium. ..
- Intestinal Calcium Absorption: Molecular MechanismJAMES FLEET; Fiscal Year: 2009..This work will lay the foundation for developing osteoporosis prevention strategies that maximize vitamin D action in the intestine and optimize the absorption of dietary calcium. ..
- Colon-specific Transgenic Mouse for Cancer ResearchJAMES FLEET; Fiscal Year: 2007..In addition, colon cancer models made with the new transgenic mouse will be useful for testing agents for their ability to treat and prevent cancer. ..
- Intestinal calcium absorption: molecular mechanismsJAMES FLEET; Fiscal Year: 2007..g. when vitamin D resistance associated with aging or estrogen deficiency is present. ..
- Vitamin D Status and Prostate CancerJAMES FLEET; Fiscal Year: 2007..These data will provide us with the mechanistic basis for dietary recommendations to prevent prostate cancer and optimize bone health. ..
- Calcium Absorption in Caco-2 Cells: Molecular MechanismJAMES FLEET; Fiscal Year: 2003..abstract_text> ..
- Intestinal Calcium Absorption: Molecular MechanismJames C Fleet; Fiscal Year: 2010..This work will lay the foundation for developing osteoporosis prevention strategies that maximize vitamin D action in the intestine and optimize the absorption of dietary calcium. ..