JAMES FLEET

Summary

Affiliation: Purdue University
Country: USA

Publications

  1. ncbi request reprint Animal models of gastrointestinal and liver diseases. New mouse models for studying dietary prevention of colorectal cancer
    James C Fleet
    Department of Nutrition Science, Purdue University, West Lafayette, Indiana and Purdue University Center for Cancer Research, Purdue University, West Lafayette, Indiana
    Am J Physiol Gastrointest Liver Physiol 307:G249-59. 2014
  2. pmc Case-control genome-wide association study of rheumatoid arthritis from Genetic Analysis Workshop 16 using penalized orthogonal-components regression-linear discriminant analysis
    Min Zhang
    Department of Statistics, Purdue University, 150 North University Street, West Lafayette, IN 47907, USA
    BMC Proc 3:S17. 2009
  3. pmc 1,25 dihydroxyvitamin D-mediated orchestration of anticancer, transcript-level effects in the immortalized, non-transformed prostate epithelial cell line, RWPE1
    Pavlo L Kovalenko
    Department of Foods and Nutrition and the Interdepartmental Nutrition Program, Purdue University, West Lafayette, IN 47907 2059, USA
    BMC Genomics 11:26. 2010
  4. ncbi request reprint What have genomic and proteomic approaches told us about vitamin D and cancer?
    James C Fleet
    Department of Foods and Nutrition, Purdue University, 700 West State St, West Lafayette, IN 47906 2059, USA
    Nutr Rev 65:S127-30. 2007
  5. ncbi request reprint Using genomics to understand intestinal biology
    J C Fleet
    Department of Foods and Nutrition and Interdepartmental Nutrition Program, Purdue University, 700 West State St, West Lafayette, IN 47906 2059, USA
    J Physiol Biochem 63:83-96. 2007
  6. ncbi request reprint Molecular regulation of calcium and bone metabolism through the vitamin D receptor
    J C Fleet
    Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907 2059, USA
    J Musculoskelet Neuronal Interact 6:336-7. 2006
  7. pmc Serum metabolite profiles and target tissue gene expression define the effect of cholecalciferol intake on calcium metabolism in rats and mice
    James C Fleet
    Department of Foods and Nutrition and the Interdepartmental Nutrition Program, Purdue University, West Lafayette, IN 47907 2059, USA
    J Nutr 138:1114-20. 2008
  8. pmc Molecular actions of vitamin D contributing to cancer prevention
    James C Fleet
    Department of Foods and Nutrition, Purdue University, 700 West State Street, West Lafayette, IN 47906 2059, USA
    Mol Aspects Med 29:388-96. 2008
  9. pmc Systems genetics of mineral metabolism
    James C Fleet
    Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47906, USA
    J Nutr 141:520-5. 2011
  10. pmc The effect of differentiation on 1,25 dihydroxyvitamin D-mediated gene expression in the enterocyte-like cell line, Caco-2
    Min Cui
    Department of Foods and Nutrition, Purdue University, West Lafayette, Indiana, USA
    J Cell Physiol 218:113-21. 2009

Research Grants

Collaborators

Detail Information

Publications41

  1. ncbi request reprint Animal models of gastrointestinal and liver diseases. New mouse models for studying dietary prevention of colorectal cancer
    James C Fleet
    Department of Nutrition Science, Purdue University, West Lafayette, Indiana and Purdue University Center for Cancer Research, Purdue University, West Lafayette, Indiana
    Am J Physiol Gastrointest Liver Physiol 307:G249-59. 2014
    ..This review will discuss how mice can be used to model human colon cancer and it will describe a variety of new mouse models that develop colon-restricted cancer as well as more advanced phenotypes for studies of late-state disease. ..
  2. pmc Case-control genome-wide association study of rheumatoid arthritis from Genetic Analysis Workshop 16 using penalized orthogonal-components regression-linear discriminant analysis
    Min Zhang
    Department of Statistics, Purdue University, 150 North University Street, West Lafayette, IN 47907, USA
    BMC Proc 3:S17. 2009
    ..In addition to the two regions on chromosomes 6 and 9 previously associated with RA by GWAS, we identified SNPs on chromosomes 10 and 18 as potential candidates for further investigation...
  3. pmc 1,25 dihydroxyvitamin D-mediated orchestration of anticancer, transcript-level effects in the immortalized, non-transformed prostate epithelial cell line, RWPE1
    Pavlo L Kovalenko
    Department of Foods and Nutrition and the Interdepartmental Nutrition Program, Purdue University, West Lafayette, IN 47907 2059, USA
    BMC Genomics 11:26. 2010
    ..Still, the molecular mechanisms and the gene targets for vitamin D-mediated prostate cancer prevention are unknown...
  4. ncbi request reprint What have genomic and proteomic approaches told us about vitamin D and cancer?
    James C Fleet
    Department of Foods and Nutrition, Purdue University, 700 West State St, West Lafayette, IN 47906 2059, USA
    Nutr Rev 65:S127-30. 2007
  5. ncbi request reprint Using genomics to understand intestinal biology
    J C Fleet
    Department of Foods and Nutrition and Interdepartmental Nutrition Program, Purdue University, 700 West State St, West Lafayette, IN 47906 2059, USA
    J Physiol Biochem 63:83-96. 2007
    ....
  6. ncbi request reprint Molecular regulation of calcium and bone metabolism through the vitamin D receptor
    J C Fleet
    Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907 2059, USA
    J Musculoskelet Neuronal Interact 6:336-7. 2006
  7. pmc Serum metabolite profiles and target tissue gene expression define the effect of cholecalciferol intake on calcium metabolism in rats and mice
    James C Fleet
    Department of Foods and Nutrition and the Interdepartmental Nutrition Program, Purdue University, West Lafayette, IN 47907 2059, USA
    J Nutr 138:1114-20. 2008
    ..Our data show that modeling human VD3 status requires lower intake than the current NRC rodent requirement (1000-IU/kg diet). Also, although rodents are very tolerant of high-VD3 intake, it cannot compensate for moderate Ca restriction...
  8. pmc Molecular actions of vitamin D contributing to cancer prevention
    James C Fleet
    Department of Foods and Nutrition, Purdue University, 700 West State Street, West Lafayette, IN 47906 2059, USA
    Mol Aspects Med 29:388-96. 2008
    ....
  9. pmc Systems genetics of mineral metabolism
    James C Fleet
    Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47906, USA
    J Nutr 141:520-5. 2011
    ..e., ionomics. This short review will explain the value of forward genetics and ionomics as tools for exploring mammalian mineral metabolism...
  10. pmc The effect of differentiation on 1,25 dihydroxyvitamin D-mediated gene expression in the enterocyte-like cell line, Caco-2
    Min Cui
    Department of Foods and Nutrition, Purdue University, West Lafayette, Indiana, USA
    J Cell Physiol 218:113-21. 2009
    ..This suggests that decreased recruitment of VDR to vitamin D response elements also contributes to the blunted transcriptional responsiveness to 1,25(OH)(2)D(3) in proliferating Caco-2 cells...
  11. ncbi request reprint Calcium transporter 1 and epithelial calcium channel messenger ribonucleic acid are differentially regulated by 1,25 dihydroxyvitamin D3 in the intestine and kidney of mice
    Yurong Song
    Interdepartmental Nutrition Program, Purdue University, West Lafayette, Indiana 47907, USA
    Endocrinology 144:3885-94. 2003
    ..The rapid induction of intestinal CaT1 mRNA expression by 1,25(OH)(2)D(3), and the marked induction at weaning, suggest that CaT1 is critical for 1,25(OH)(2)D(3)-mediated intestinal Ca absorption...
  12. pmc Control of differentiation-induced calbindin-D9k gene expression in Caco-2 cells by cdx-2 and HNF-1alpha
    Liyong Wang
    Interdepartmental Nutrition Program, Purdue University, West Lafayette, Indiana 47907, USA
    Am J Physiol Gastrointest Liver Physiol 287:G943-53. 2004
    ..Our data suggest that cdx-2 is a permissive factor that influences basal CaBP expression in enterocytes and that HNF-1alpha modulates CaBP gene expression during cellular differentiation...
  13. ncbi request reprint Vitamin D receptor (VDR) knockout mice reveal VDR-independent regulation of intestinal calcium absorption and ECaC2 and calbindin D9k mRNA
    Yurong Song
    Interdepartmental Nutrition Program, Purdue University, West Lafayette, IN 47907, USA
    J Nutr 133:374-80. 2003
    ..In addition, our data suggest that the 2% Ca diet promotes a vitamin D receptor-independent anabolic effect on bone formation and calcium absorption, leading to improved calcium balance even in the presence of high PTH levels...
  14. ncbi request reprint 1,25 dihydroxycholecalciferol-mediated calcium absorption and gene expression are higher in female than in male mice
    Yurong Song
    Department of Foods and Nutrition and the Interdepartmental Nutrition Program, Purdue University, West Lafayette, IN 47907, USA
    J Nutr 134:1857-61. 2004
    ..Our data indicate that female mice are more sensitive to changes in serum 1,25(OH)(2) D levels than males and that this must be considered when using mice to study calcium and bone biology...
  15. pmc Simultaneous genome-wide association studies of anti-cyclic citrullinated peptide in rheumatoid arthritis using penalized orthogonal-components regression
    Yanzhu Lin
    Department of Statistics, Purdue University, West Lafayette, Indiana 47907, USA YL
    BMC Proc 3:S20. 2009
    ..Bioinformatic analysis of these genomic regions showed most of them harbor protein-coding gene(s)...
  16. pmc Short-term low-protein intake does not increase serum parathyroid hormone concentration in humans
    Wayne W Campbell
    Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907, USA
    J Nutr 134:1900-4. 2004
    ..These findings do not support the hypothesis that the short-term ingestion of inadequate dietary protein increases serum PTH concentration...
  17. pmc Effects of MAPK signaling on 1,25-dihydroxyvitamin D-mediated CYP24 gene expression in the enterocyte-like cell line, Caco-2
    Min Cui
    Department of Foods and Nutrition, Purdue University, West Lafayette, Indiana 47906 2059, USA
    J Cell Physiol 219:132-42. 2009
    ..Our data show that there are both promoter-specific and cell stage-specific roles for the ERK signaling pathway on 1,25(OH)(2)D(3)-mediated gene induction in enterocyte-like Caco-2 cells...
  18. ncbi request reprint Vitamin D-inducible calcium transport and gene expression in three Caco-2 cell lines
    James C Fleet
    Department of Foods and Nutrition, Purdue University, West Lafayette, Indiana 47906, USA
    Am J Physiol Gastrointest Liver Physiol 283:G618-25. 2002
    ..Basal CaBP mRNA (86 times greater than P) and protein levels were highest in TC7 cells and were not associated with higher net CaTx, suggesting CaBP may not be rate limiting for CaTx in these cells...
  19. ncbi request reprint Gene expression profiling of Caco-2 BBe cells suggests a role for specific signaling pathways during intestinal differentiation
    James C Fleet
    Interdepartmental Nutrition Program, Purdue University, West Lafayette, Indiana 47907
    Physiol Genomics 13:57-68. 2003
    ....
  20. ncbi request reprint Genomic and proteomic approaches for probing the role of vitamin D in health
    James C Fleet
    Interdepartmental Nutrition Program and Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907 2059, USA
    Am J Clin Nutr 80:1730S-4S. 2004
    ....
  21. ncbi request reprint Vitamin D requirements: current and future
    Connie M Weaver
    Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907 2059, USA
    Am J Clin Nutr 80:1735S-9S. 2004
    ..So much new information on vitamin D and health has been collected since the requirements were set in 1997 that this nutrient is likely the most in need of revised requirements...
  22. pmc Intestinal vitamin D receptor is required for normal calcium and bone metabolism in mice
    Yingben Xue
    Department of Foods and Nutrition, Purdue University, West Lafayette, Indiana 47906 2059, USA
    Gastroenterology 136:1317-27, e1-2. 2009
    ..To specifically study the effects of VDR in intestinal calcium absorption, we investigated whether restoration of intestinal VDR is sufficient to recover the phenotype of VDR-knockout mice...
  23. pmc Intestinal resistance to 1,25 dihydroxyvitamin D in mice heterozygous for the vitamin D receptor knockout allele
    Yurong Song
    Interdepartmental Nutrition Program and Department of Foods and Nutrition, Purdue University, 700 West State Street, West Lafayette, IN 47906 2059, USA
    Endocrinology 148:1396-402. 2007
    ..Our data show that low VDR levels lead to resistance of intestinal Ca absorption to 1,25(OH)(2) D(3), and this resistance may be due to a role for the VDR (and VDR level) in the translation of CaBP...
  24. pmc Inadequate protein intake affects skeletal muscle transcript profiles in older humans
    Anna E Thalacker-Mercer
    Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907, USA
    Am J Clin Nutr 85:1344-52. 2007
    ..Inadequate dietary protein intake causes adverse changes in the morphology and function of skeletal muscle. These changes may be reflected in early alterations in muscle messenger RNA levels...
  25. pmc Sodium-dependent phosphate uptake in the jejunum is post-transcriptionally regulated in pigs fed a low-phosphorus diet and is independent of dietary calcium concentration
    Kari L Saddoris
    Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA
    J Nutr 140:731-6. 2010
    ..These data suggest that restricting dietary aP concentration by only 43% stimulates Na(+)-dependent phosphate uptake and expression of the NaPi-IIb protein in the BBM of the small intestine and through a post-transcriptional mechanism...
  26. pmc Activation of rapid signaling pathways does not contribute to 1 alpha,25-dihydroxyvitamin D3-induced growth inhibition of mouse prostate epithelial progenitor cells
    Jia Li
    Interdepartmental Nutrition Program, Purdue University, 700 W State Street, West Lafayette, Indiana 47907, USA
    J Cell Biochem 107:1031-6. 2009
    ..In conclusion, 1,25(OH)(2)D did not mediate rapid activation of ERK1/2 or PKC alpha in MPEC3 and therefore the growth inhibitory effect of 1,25(OH)(2)D is independent of rapid activation of these signaling pathways in this cell type...
  27. pmc Constitutive activation of the mitogen-activated protein kinase pathway impairs vitamin D signaling in human prostate epithelial cells
    Zhentao Zhang
    Department of Foods and Nutrition, Purdue University, West Lafayette, Indiana 47907 2059, USA
    J Cell Physiol 224:433-42. 2010
    ..This is due in part to the phosphorylation of critical amino acids in the RXRalpha AF-1 domain and impaired co-activator recruitment...
  28. ncbi request reprint Rapid, membrane-initiated actions of 1,25 dihydroxyvitamin D: what are they and what do they mean?
    James C Fleet
    Department of Foods and Nutrition and the Interdepartmental Nutrition Program, Purdue University, West Lafayette, IN 47907, USA
    J Nutr 134:3215-8. 2004
    ..This review summarizes recent research on the rapid actions of 1,25(OH)(2) D and identifies questions that remain to be answered in this area...
  29. pmc Nucleo-cytoplasmic cycling of the vitamin D receptor in the enterocyte-like cell line, Caco-2
    Anna Klopot
    Department of Foods and Nutrition and the Interdepartmental Nutrition Program, Purdue University, West Lafayette, IN 47907 2059, USA
    J Cell Biochem 100:617-28. 2007
    ..In addition, ligand-dependent VDR nuclear import appears to be balanced by export, thus accounting for the lack of nuclear VDR accumulation even when VDR import is significantly elevated...
  30. ncbi request reprint Effects of hindlimb unloading and bisphosphonates on the serum proteome of rats
    Yongdong Zhao
    Department of Foods and Nutrition, Purdue University, 700 W State St, West Lafayette, IN 47907 2059, USA
    Bone 41:646-58. 2007
    ..e. drug related), and 17 common to HU and HUA (e.g. potential mental stress or muscle loss markers). Spots were identified by LC-MS/MS after in-gel trypsin digestion and were found to relate to a variety of physiological functions...
  31. pmc Dietary vitamin D and vitamin D receptor level modulate epithelial cell proliferation and apoptosis in the prostate
    Pavlo L Kovalenko
    Center for Cancer Research, Department of Foods and Nutrition, Purdue University, West Lafayette, Indiana, USA
    Cancer Prev Res (Phila) 4:1617-25. 2011
    ..Our data show that low diet VDR or VDR deletion provide a prostate environment that is permissive to early procarcinogenic events that enhance prostate cancer risk...
  32. pmc Generation of a transgenic mouse for colorectal cancer research with intestinal cre expression limited to the large intestine
    Yingben Xue
    Purdue University, West Lafayette, IN 47906 2059, USA
    Mol Cancer Res 8:1095-104. 2010
    ..Our new model has improved features for colon cancer research, that is, transgene expression is limited to the epithelium of the large bowel with normal cells found next to genetically modified cells...
  33. pmc Forward genetics used to identify new gene Mon1a with critical role in controlling macrophage iron metabolism and iron recycling from erythrocytes
    Rebecca A McCreedy
    Department of Foods and Nutrition, and Center for Gene Environment Interactions, Purdue University, West Lafayette, Indiana 47906 2059, USA
    Nutr Rev 67:607-10. 2009
    ..The forward genetics approach, which has currently only been used sparingly by the nutrition research community, offers a powerful and unbiased approach to identifying genes important in nutritional metabolism...
  34. pmc High dietary vitamin D prevents hypocalcemia and osteomalacia in CYP27B1 knockout mice
    Matthew J Rowling
    Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
    J Nutr 137:2608-15. 2007
    ..However, neither high dietary Ca nor high dietary VD3 is sufficient to fully recover the phenotype of CYP27B1 KO mice...
  35. ncbi request reprint Effect of cellular environment on the selective activation of the vitamin D receptor by 1alpha,25-dihydroxyvitamin D3 and its analog 1alpha-fluoro-16-ene-20-epi-23-ene-26,27-bishomo-25-hydroxyvitamin D3 (Ro-26-9228)
    Ayesha Ismail
    Department of Endocrine Neoplasia and Hormonal Disorders, Unit 435, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Mol Endocrinol 18:874-87. 2004
    ..These results suggest that Ro-26-9228 has poor ability to promote the accumulation of a proteasome-sensitive, transcriptionally active VDR isoform in Caco-2 cells, whereas it does not have this limitation in hFOB cells...
  36. ncbi request reprint 1,25-Dihydroxyvitamin D and 25-hydroxyvitamin D--mediated regulation of TRPV6 (a putative epithelial calcium channel) mRNA expression in Caco-2 cells
    Shveta Taparia
    Mineral Bioavailability Laboratory, Jean Mayer USDA Human Nutrition, Research Center on Aging, Tufts University, 711 Washington Street, Boston MA 02111, USA
    Eur J Nutr 45:196-204. 2006
    ..TRPV6 mRNA expression is strongly regulated by 1,25-dihydroxyvitamin D (1,25VD), the active hormonal form of vitamin D, in intestine and in Caco-2 cells, a human colon cancer cell line...
  37. ncbi request reprint Excentric cleavage products of beta-carotene inhibit estrogen receptor positive and negative breast tumor cell growth in vitro and inhibit activator protein-1-mediated transcriptional activation
    Elmi C Tibaduiza
    Molecular Pharmacology Research Center, New England Medical Center, Boston, MA 02111, USA
    J Nutr 132:1368-75. 2002
    ....
  38. ncbi request reprint Transgenic expression of the human Vitamin D receptor (hVDR) in the duodenum of VDR-null mice attenuates the age-dependent decline in calcium absorption
    Hilary D Marks
    Department of Endocrine Neoplasia and Hormonal Disorders, Unit 435, The University of Texas, M D Anderson Cancer Center, Houston, TX 77030, USA
    J Steroid Biochem Mol Biol 103:513-6. 2007
    ..In addition, hVDR+/mVDR-null mice responded better to the late rescue diet suggesting that expression of VDR in the proximal small intestine protected the calcium absorbing machinery from age-dependent decline...
  39. ncbi request reprint Dairy consumption and the prevention of colon cancer: is there more to the story than calcium?
    James C Fleet
    Am J Clin Nutr 83:527-8. 2006
  40. ncbi request reprint Renal cell cancer and nuclear receptor levels--biomarkers or functionally relevant?
    James C Fleet
    J Urol 178:1144-5. 2007
  41. ncbi request reprint Vitamin D receptor alleles, periodontal disease progression, and tooth loss in the VA dental longitudinal study
    Koji Inagaki
    Department of Health Policy and Health Services Research, Boston University Goldman School of Dental Medicine, Boston, MA, USA
    J Periodontol 74:161-7. 2003
    ..The relationship between the vitamin D receptor (VDR) genotype and periodontal disease is not known. This study compared periodontal disease progression among polymorphisms of 2 VDR genes in men in the VA Dental Longitudinal Study...

Research Grants16

  1. Intestinal Calcium Absorption: Molecular Mechanism
    James C Fleet; Fiscal Year: 2010
    ..This work will lay the foundation for developing osteoporosis prevention strategies that maximize vitamin D action in the intestine and optimize the absorption of dietary calcium. ..
  2. Intestinal Calcium Absorption: Molecular Mechanism
    JAMES FLEET; Fiscal Year: 2009
    ..This work will lay the foundation for developing osteoporosis prevention strategies that maximize vitamin D action in the intestine and optimize the absorption of dietary calcium. ..
  3. Colon-specific Transgenic Mouse for Cancer Research
    JAMES FLEET; Fiscal Year: 2007
    ..In addition, colon cancer models made with the new transgenic mouse will be useful for testing agents for their ability to treat and prevent cancer. ..
  4. Intestinal calcium absorption: molecular mechanisms
    JAMES FLEET; Fiscal Year: 2007
    ..g. when vitamin D resistance associated with aging or estrogen deficiency is present. ..
  5. Vitamin D Status and Prostate Cancer
    JAMES FLEET; Fiscal Year: 2007
    ..These data will provide us with the mechanistic basis for dietary recommendations to prevent prostate cancer and optimize bone health. ..
  6. Intestinal calcium absorption: molecular mechanisms
    JAMES FLEET; Fiscal Year: 2006
    ..g. when vitamin D resistance associated with aging or estrogen deficiency is present. ..
  7. Vitamin D Status and Prostate Cancer
    JAMES FLEET; Fiscal Year: 2006
    ..These data will provide us with the mechanistic basis for dietary recommendations to prevent prostate cancer and optimize bone health. ..
  8. Vitamin D Status and Prostate Cancer
    JAMES FLEET; Fiscal Year: 2005
    ..These data will provide us with the mechanistic basis for dietary recommendations to prevent prostate cancer and optimize bone health. ..
  9. Intestinal calcium absorption: molecular mechanisms
    JAMES FLEET; Fiscal Year: 2005
    ..g. when vitamin D resistance associated with aging or estrogen deficiency is present. ..
  10. Intestinal calcium absorption: molecular mechanisms
    JAMES FLEET; Fiscal Year: 2004
    ..g. when vitamin D resistance associated with aging or estrogen deficiency is present. ..
  11. Vitamin D Status and Prostate Cancer
    JAMES FLEET; Fiscal Year: 2004
    ..These data will provide us with the mechanistic basis for dietary recommendations to prevent prostate cancer and optimize bone health. ..
  12. Calcium Absorption in Caco-2 Cells: Molecular Mechanism
    JAMES FLEET; Fiscal Year: 2003
    ..abstract_text> ..
  13. Intestinal Calcium Absorption: Molecular Mechanism
    James C Fleet; Fiscal Year: 2010
    ..This work will lay the foundation for developing osteoporosis prevention strategies that maximize vitamin D action in the intestine and optimize the absorption of dietary calcium. ..