W A Cramer

Summary

Affiliation: Purdue University
Country: USA

Publications

  1. pmc Membrane proteins in four acts: function precedes structure determination
    W A Cramer
    Department of Biological Sciences, Purdue University, Hall of Structural Biology, 240 Hockmeyer Hall, West Lafayette, IN 47907 1354, USA
    Methods 55:415-20. 2011
  2. pmc On rate limitations of electron transfer in the photosynthetic cytochrome b6f complex
    S Saif Hasan
    Department of Biological Sciences, Hockmeyer Hall of Structural Biology, Purdue University, West Lafayette, IN 47907, USA
    Phys Chem Chem Phys 14:13853-60. 2012
  3. pmc The Q cycle of cytochrome bc complexes: a structure perspective
    William A Cramer
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
    Biochim Biophys Acta 1807:788-802. 2011
  4. ncbi request reprint Binding sites of lipophilic quinone and quinone analogue inhibitors in the cytochrome b6f complex of oxygenic photosynthesis
    W A Cramer
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 2154, USA
    Biochem Soc Trans 33:921-3. 2005
  5. ncbi request reprint Consequences of the structure of the cytochrome b6f complex for its charge transfer pathways
    William A Cramer
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
    Biochim Biophys Acta 1757:339-45. 2006
  6. ncbi request reprint Transmembrane traffic in the cytochrome b6f complex
    William A Cramer
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 2054, USA
    Annu Rev Biochem 75:769-90. 2006
  7. ncbi request reprint Structure of the cytochrome b6f complex of oxygenic photosynthesis: tuning the cavity
    Genji Kurisu
    Department of Biological Sciences, 915 West State Street, Purdue University, West Lafayette, IN 47907 2054, USA
    Science 302:1009-14. 2003
  8. ncbi request reprint Structure of the complex of the colicin E2 R-domain and its BtuB receptor. The outer membrane colicin translocon
    Onkar Sharma
    Department of Biological Sciences, Lilly Hall of Life Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Biol Chem 282:23163-70. 2007
  9. doi request reprint Primary events in the colicin translocon: FRET analysis of colicin unfolding initiated by binding to BtuB and OmpF
    Stanislav D Zakharov
    Department of Biological Sciences and Purdue Cancer Center, Purdue University, West Lafayette, Indiana 47907 2054, USA
    Biochemistry 47:12802-9. 2008
  10. ncbi request reprint Colicin crystal structures: pathways and mechanisms for colicin insertion into membranes
    Stanislav D Zakharov
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 1392, USA
    Biochim Biophys Acta 1565:333-46. 2002

Collaborators

Detail Information

Publications54

  1. pmc Membrane proteins in four acts: function precedes structure determination
    W A Cramer
    Department of Biological Sciences, Purdue University, Hall of Structural Biology, 240 Hockmeyer Hall, West Lafayette, IN 47907 1354, USA
    Methods 55:415-20. 2011
    ....
  2. pmc On rate limitations of electron transfer in the photosynthetic cytochrome b6f complex
    S Saif Hasan
    Department of Biological Sciences, Hockmeyer Hall of Structural Biology, Purdue University, West Lafayette, IN 47907, USA
    Phys Chem Chem Phys 14:13853-60. 2012
    ....
  3. pmc The Q cycle of cytochrome bc complexes: a structure perspective
    William A Cramer
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
    Biochim Biophys Acta 1807:788-802. 2011
    ....
  4. ncbi request reprint Binding sites of lipophilic quinone and quinone analogue inhibitors in the cytochrome b6f complex of oxygenic photosynthesis
    W A Cramer
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 2154, USA
    Biochem Soc Trans 33:921-3. 2005
    ..The discussion is focused on the binding sites of plastoquinone and quinone analogue inhibitors discerned in the structure. These sites mark the pathway(s) of quinone translocation across the complex...
  5. ncbi request reprint Consequences of the structure of the cytochrome b6f complex for its charge transfer pathways
    William A Cramer
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
    Biochim Biophys Acta 1757:339-45. 2006
    ..Thus, either regulation is required at the level of the redox state of the hemes that would allow them to be shared by the two pathways, and/or the two different pathways are segregated in the membrane...
  6. ncbi request reprint Transmembrane traffic in the cytochrome b6f complex
    William A Cramer
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 2054, USA
    Annu Rev Biochem 75:769-90. 2006
    ..It is hypothesized that this heme, exposed to the cavity and a neighboring plastoquinone and close to the positive surface potential of the complex, can function in cyclic electron transport via anionic ferredoxin...
  7. ncbi request reprint Structure of the cytochrome b6f complex of oxygenic photosynthesis: tuning the cavity
    Genji Kurisu
    Department of Biological Sciences, 915 West State Street, Purdue University, West Lafayette, IN 47907 2054, USA
    Science 302:1009-14. 2003
    ..The motion of the Rieske iron-sulfur protein extrinsic domain, essential for electron transfer, must also be different in the b6f complex...
  8. ncbi request reprint Structure of the complex of the colicin E2 R-domain and its BtuB receptor. The outer membrane colicin translocon
    Onkar Sharma
    Department of Biological Sciences, Lilly Hall of Life Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Biol Chem 282:23163-70. 2007
    ..Arg(452) at the R/C-domain interface in colicin E2 was found have an essential role at a putative site of protease cleavage, which would liberate the C-terminal activity domain for passage through the outer membrane translocon...
  9. doi request reprint Primary events in the colicin translocon: FRET analysis of colicin unfolding initiated by binding to BtuB and OmpF
    Stanislav D Zakharov
    Department of Biological Sciences and Purdue Cancer Center, Purdue University, West Lafayette, Indiana 47907 2054, USA
    Biochemistry 47:12802-9. 2008
    ..The R-domain Arg399 is responsible for electrostatic interaction with BtuB. It is concluded that free energy for colicin unfolding is provided by binding of the R- domain to BtuB and binding/insertion of the T-domain to/into OmpF...
  10. ncbi request reprint Colicin crystal structures: pathways and mechanisms for colicin insertion into membranes
    Stanislav D Zakharov
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 1392, USA
    Biochim Biophys Acta 1565:333-46. 2002
    ..Helix elongation contradicts suggestions that the colicin import involves a molten globule intermediate. The nature of the open-channel structure is discussed...
  11. pmc The single chlorophyll a molecule in the cytochrome b6f complex: unusual optical properties protect the complex against singlet oxygen
    Naranbaatar Dashdorj
    Department of Physics, Purdue University, West Lafayette, Indiana 47907, USA
    Biophys J 88:4178-87. 2005
    ..It is also reported that the Chl a molecule in the cytochrome b(6)f complex does not change orientation in its excited state...
  12. ncbi request reprint Purification and crystallization of the cytochrome b6f complex in oxygenic photosynthesis
    Huamin Zhang
    Department of Biological Sciences, Lilly Hall of Life Sciences, Purdue University, West Lafayette, IN, USA
    Methods Mol Biol 274:67-78. 2004
    ..2 mm) crystals suitable for x-ray diffraction analysis and structure determination, which diffract with good statistics to 3.0 A...
  13. pmc Crystal structures of the OmpF porin: function in a colicin translocon
    Eiki Yamashita
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 1392, USA
    EMBO J 27:2171-80. 2008
    ..Involvement of OmpF in colicin passage through the OM was further documented by immuno-extraction of an OM complex, the colicin translocon, consisting of colicin E3, BtuB and OmpF...
  14. pmc Minimum length requirement of the flexible N-terminal translocation subdomain of colicin E3
    Onkar Sharma
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
    J Bacteriol 189:363-8. 2007
    ....
  15. ncbi request reprint The structure of BtuB with bound colicin E3 R-domain implies a translocon
    Genji Kurisu
    Department of Biological Sciences, Purdue University, Lilly Hall of Life Sciences, 915 W State St, West Lafayette, Indiana 47907 1392, USA
    Nat Struct Biol 10:948-54. 2003
    ..The translocator was concluded to be OmpF from the occlusion of OmpF channels by colicin E3...
  16. ncbi request reprint The colicin E3 outer membrane translocon: immunity protein release allows interaction of the cytotoxic domain with OmpF porin
    Stanislav D Zakharov
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    Biochemistry 45:10199-207. 2006
    ....
  17. ncbi request reprint Deuterium kinetic isotope effects in the p-side pathway for quinol oxidation by the cytochrome b(6)f complex
    G M Soriano
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    Biochemistry 40:15109-16. 2001
    ....
  18. pmc Structure of the cytochrome b6f complex: quinone analogue inhibitors as ligands of heme cn
    E Yamashita
    Department of Biological Sciences, Purdue University, 915 West State St, West Lafayette, IN 47907, USA
    J Mol Biol 370:39-52. 2007
    ..A decreased extent of heme c(n) reduction by reduced ferredoxin in the presence of NQNO allows observation of the heme c(n) Soret band in a chemical difference spectrum...
  19. ncbi request reprint Ferredoxin:NADP+ oxidoreductase is a subunit of the chloroplast cytochrome b6f complex
    H Zhang
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA
    J Biol Chem 276:38159-65. 2001
    ..It was inferred that the b6f complex with bound FNR, one equivalent per monomer, provides the membrane protein connection to the main electron transfer chain for ferredoxin-dependent cyclic electron transport...
  20. pmc A defined protein-detergent-lipid complex for crystallization of integral membrane proteins: The cytochrome b6f complex of oxygenic photosynthesis
    Huamin Zhang
    Department of Biological Sciences, Lilly Hall of Life Sciences, Purdue University, West Lafayette, IN 47907 2054, USA
    Proc Natl Acad Sci U S A 100:5160-3. 2003
    ....
  21. doi request reprint Structure-function of the cytochrome b6f complex
    D Baniulis
    Department of Biological Sciences, Purdue University, West Lafayette, IN, USA
    Photochem Photobiol 84:1349-58. 2008
    ....
  22. ncbi request reprint Characterization and crystallization of the lumen side domain of the chloroplast Rieske iron-sulfur protein
    H Zhang
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 1392, USA
    J Biol Chem 271:31360-6. 1996
    ..1 A, b = 31.9 A, c = 35.8 A, alpha = 95.6 degrees, beta = 107.1 degrees, gamma = 117.3 degrees...
  23. ncbi request reprint Functional insensitivity of the cytochrome b6f complex to structure changes in the hinge region of the Rieske iron-sulfur protein
    Jiusheng Yan
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 2054, USA
    J Biol Chem 278:20925-33. 2003
    ..This implies that either the barriers to motion or the amplitude of ISP motion required for function is smaller than in the bc1 complex...
  24. pmc Structure-Function, Stability, and Chemical Modification of the Cyanobacterial Cytochrome b6f Complex from Nostoc sp. PCC 7120
    Danas Baniulis
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Biol Chem 284:9861-9. 2009
    ..The high spin electronic character of the unique heme c(n) is similar to that previously found in the b(6)f complex from other sources...
  25. pmc Identification of specific residues in colicin E1 involved in immunity protein recognition
    M Lindeberg
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47906 1392, USA
    J Bacteriol 183:2132-6. 2001
    ..Mutagenesis of helix VI residues 416 to 419 in Col10 to the corresponding ColE1 sequence resulted in increased recognition by Imm and loss of recognition by Cti...
  26. ncbi request reprint Helical alpha-synuclein forms highly conductive ion channels
    Stanislav D Zakharov
    Departments of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 2054, USA
    Biochemistry 46:14369-79. 2007
    ....
  27. ncbi request reprint Ultrafast optical pump-probe studies of the cytochrome b(6)f complex in solution and crystalline states
    Naranbaatar Dashdorj
    Department of Physics and Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Phys Chem B 111:14405-10. 2007
    ..This supports an earlier hypothesis that the short excited state lifetime of Chl a is critical for the function of the b6f complex...
  28. ncbi request reprint Problems in obtaining diffraction-quality crystals of hetero-oligomeric integral membrane proteins
    Huamin Zhang
    Department of Biological Sciences, Lilly Hall of Life Sciences, Purdue University, West Lafayette, IN 47907 2054, USA
    J Struct Funct Genomics 6:219-23. 2005
    ..In the case of the complex formed by the cobalamin and colicin receptor, BtuB, and the receptor binding domain of colicin E3, the latter served as a ligand for protein-protein contacts that facilitated crystallization...
  29. pmc Colicin occlusion of OmpF and TolC channels: outer membrane translocons for colicin import
    Stanislav D Zakharov
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA
    Biophys J 87:3901-11. 2004
    ..These data are considered in the context of a two-receptor translocon model for colicin entry into cells...
  30. ncbi request reprint Cytochrome bc complexes: a common core of structure and function surrounded by diversity in the outlying provinces
    Janet L Smith
    Department of Biological Sciences, 915 West State Street, Purdue University, West Lafayette, Indiana 47907 2054, USA
    Curr Opin Struct Biol 14:432-9. 2004
    ....
  31. ncbi request reprint Evolution of photosynthesis: time-independent structure of the cytochrome b6f complex
    William A Cramer
    Department of Biological Sciences, Lilly Hall of Life Sciences, 915 West State Street, Purdue University, West Lafayette, Indiana 47907 2054, USA
    Biochemistry 43:5921-9. 2004
    ..However, in the cyanobacterial complex, TDS is in an orientation that is the opposite of its position in the algal structure and bc(1) complexes, so its headgroup in the M. laminosus structure is 20 A from the Fe(2)S(2) cluster...
  32. ncbi request reprint On the mechanism and pathway of colicin import across the E. Coli outer membrane
    Stanislav D Zakharov
    Department of Biological Sciences, Lilly Hall of Life Sciences, Purdue University, West Lafayette, IN 47907 1392, USA
    Front Biosci 9:1311-7. 2004
    ....
  33. ncbi request reprint Characterization of the high-spin heme x in the cytochrome b6f complex of oxygenic photosynthesis
    Huamin Zhang
    Department of Biological Sciences, Lilly Hall of Life Sciences, Purdue University, West Lafayette, Indiana 47907 2054, USA
    Biochemistry 43:16329-36. 2004
    ..In addition to a possible function in photosystem I-linked cyclic electron transport, the five-coordinate state implies that there is at least one more function of heme x that is related to axial binding of a physiological ligand...
  34. pmc Tuning the membrane surface potential for efficient toxin import
    Stanislav D Zakharov
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 1392, USA
    Proc Natl Acad Sci U S A 99:8654-9. 2002
    ..This suggests that one reason the membrane surface potential is tuned in vivo is to facilitate protein import...
  35. ncbi request reprint Molecular control of a bimodal distribution of quinone-analogue inhibitor binding sites in the cytochrome b(6)f complex
    Jiusheng Yan
    Department of Biological Sciences, Lilly Hall of Life Sciences, Purdue University, 915 W State Street, West Lafayette, IN 47907 2054, USA
    J Mol Biol 344:481-93. 2004
    ....
  36. ncbi request reprint Insertion intermediates of pore-forming colicins in membrane two-dimensional space
    S D Zakharov
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 1392, USA
    Biochimie 84:465-75. 2002
    ..Thus, the membrane interfacial layer, with the CP backbone situated near the acyl chain carbonyls, provides a favorable environment for the structure changes necessary for the transition from the soluble to the membrane-inserted state...
  37. ncbi request reprint Topography of the chloroplast cytochrome b6: orientation of the cytochrome and accessibility of the lumen-side interhelix loops
    A Szczepaniak
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907
    Z Naturforsch C 44:453-61. 1989
    ..abstract truncated at 250 words)..
  38. ncbi request reprint Structure of the cytochrome b6f complex: new prosthetic groups, Q-space, and the 'hors d'oeuvres hypothesis' for assembly of the complex
    William A Cramer
    Department of Biological Sciences, Lilly Hall of Life Sciences, Purdue University, 915 West State Street, West Lafayette, IN 47907 2054, USA
    Photosynth Res 85:133-43. 2005
    ....
  39. ncbi request reprint On the role of lipid in colicin pore formation
    Stanislav D Zakharov
    Department of Biological Sciences, Lilly Hall of Life Sciences, Purdue University, West Lafayette, IN 47907 1392, USA
    Biochim Biophys Acta 1666:239-49. 2004
    ..The resulting pore structure may consist of somewhat short, ca. 16 residues, trans-membrane helices, in a locally thinned membrane, together with surface elements of inverted phase lipid micelles...
  40. pmc Intraprotein transfer of the quinone analogue inhibitor 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone in the cytochrome b6f complex
    Jiusheng Yan
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
    Proc Natl Acad Sci U S A 103:69-74. 2006
    ..Together with the necessary passage of quinone/quinol through the small Q(p) portal in the complex, it is seen that transmembrane traffic of quinone-like molecules through the core of cytochrome bc complexes can be labyrinthine...
  41. pmc The TolA protein interacts with colicin E1 differently than with other group A colicins
    S L Schendel
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 1392, USA
    J Bacteriol 179:3683-90. 1997
    ..Possible explanations for the distinct interaction and unique translocation mechanism of colicin E1 are discussed...
  42. ncbi request reprint Electron transfer from the Rieske iron-sulfur protein (ISP) to cytochrome f in vitro. Is a guided trajectory of the ISP necessary for competent docking?
    Glenda M Soriano
    Department of Biological Sciences and Program in Biochemistry Molecular Biology, Purdue University, West Lafayette, Indiana 47907 1392, USA
    J Biol Chem 277:41865-71. 2002
    ..The requirement of a specific electrically neutral docking configuration for ISP electron transfer is consistent with structure data for the related cytochrome bc(1) complex...
  43. doi request reprint On the structural role of the aromatic residue environment of the chlorophyll a in the cytochrome b6f complex
    Jiusheng Yan
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    Biochemistry 47:3654-61. 2008
    ....
  44. ncbi request reprint Fe vibrational spectroscopy of myoglobin and cytochrome f
    Kristl L Adams
    Department of Physics, Purdue University, West Lafayette, Indiana 47907, USA
    J Phys Chem B 110:530-6. 2006
    ..The protein's contribution to Fe motion is dominant at low frequencies, where coupling to the backbone tightly constrains Fe displacements in cytochrome f, in contrast to enhanced heme flexibility in myoglobin...
  45. pmc Genome-wide screens: novel mechanisms in colicin import and cytotoxicity
    Onkar Sharma
    Department of Biological Sciences, Lilly Hall of Life Sciences, Purdue University, West Lafayette, IN 47907, USA
    Mol Microbiol 73:571-85. 2009
    ....
  46. pmc An anomalous distance dependence of intraprotein chlorophyll-carotenoid triplet energy transfer
    Hanyoup Kim
    Biophys J 89:L28-30. 2005
  47. ncbi request reprint Full subunit coverage liquid chromatography electrospray ionization mass spectrometry (LCMS+) of an oligomeric membrane protein: cytochrome b(6)f complex from spinach and the cyanobacterium Mastigocladus laminosus
    Julian P Whitelegge
    The Pasarow Mass Spectrometry Laboratory, Department of Psychiatry, Neuropsychiatric Institute, University of California, Los Angeles, California 90095, USA
    Mol Cell Proteomics 1:816-27. 2002
    ..Full subunit coverage of an oligomeric intrinsic membrane protein complex by LCMS+ presents a new facet to intact mass proteomics...
  48. ncbi request reprint [Molecular mechanism of protein import through receptor complexes: implication of the complex structure of outer membrane receptor and colicin E3]
    Kurisu Genji
    Tanpakushitsu Kakusan Koso 50:356-61. 2005
  49. ncbi request reprint Effect of lipids with different spontaneous curvature on the channel activity of colicin E1: evidence in favor of a toroidal pore
    Alexander A Sobko
    A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Moscow 119992, Russia
    FEBS Lett 576:205-10. 2004
    ..It is inferred that the toroidal pore model previously validated for small antimicrobial peptides is applicable to colicin E1, a large protein that contains ten alpha-helices in its pore-forming domain...
  50. ncbi request reprint Lipid dependence of the channel properties of a colicin E1-lipid toroidal pore
    Alexander A Sobko
    A N Belozersky Institute of Physico Chemical Biology, Moscow State University, 119992 Moscow, Russia
    J Biol Chem 281:14408-16. 2006
    ....
  51. ncbi request reprint [Structure and function of the cytochrome b6f complex of oxygenic photosynthesis]
    Genji Kurisu
    Tanpakushitsu Kakusan Koso 49:1265-73. 2004
  52. ncbi request reprint Sequencing covalent modifications of membrane proteins
    Julian P Whitelegge
    The Pasarow Mass Spectrometry Laboratory, and the Jane and Terry Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, 405 Hilgard Avenue, Los Angeles, CA 90095, USA
    J Exp Bot 57:1515-22. 2006
    ....
  53. ncbi request reprint Membrane protein crystallization in lipidic mesophases with tailored bilayers
    Lisa V Misquitta
    Department of Chemistry, The Ohio State University, Columbus, OH 43210, USA
    Structure 12:2113-24. 2004
    ..The latter is the first beta barrel protein to be crystallized by the in meso method. Protein stability in the short-chain lipid and how this relates to crystallogenesis are discussed...
  54. pmc Heme-heme interactions in the cytochrome b6f complex: EPR spectroscopy and correlation with structure
    Anna I Zatsman
    Department of Chemistry, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA
    J Am Chem Soc 128:14246-7. 2006
    ..The electronic coupling implies that the heme bn/cn pair could function as a unit to facilitate 2-electron reduction of plastoquionone without generation of an energetically unfavorable semiquinone intermediate...

Research Grants1

  1. Problems in Membrane Protein Crystallography: Hetero-Oligomeric Cytochrome b6f
    William A Cramer; Fiscal Year: 2010
    ..Via the membrane, the set of energy-transducing proteins determines the level of energy and its regulation in the human cell. ..