Steven S Broyles

Summary

Affiliation: Purdue University
Country: USA

Publications

  1. ncbi request reprint Vaccinia virus transcription
    Steven S Broyles
    Department of Biochemistry, Purdue University, West Lafayette, IN 47907 1153, USA
    J Gen Virol 84:2293-303. 2003
  2. pmc Antiviral activity of distamycin A against vaccinia virus is the result of inhibition of postreplicative mRNA synthesis
    Steven S Broyles
    Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907 1153, USA
    J Virol 78:2137-41. 2004
  3. pmc The age of reverse biochemistry
    Steven S Broyles
    Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA
    Protein Sci 16:2-3. 2007
  4. pmc Vaccinia virus intermediate and late promoter elements are targeted by the TATA-binding protein
    Bruce A Knutson
    Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA
    J Virol 80:6784-93. 2006
  5. doi request reprint Downregulation of vaccinia virus intermediate and late promoters by host transcription factor YY1
    Bruce A Knutson
    Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA
    J Gen Virol 90:1592-9. 2009
  6. doi request reprint Bidirectional transcriptional promoters in the vaccinia virus genome
    Bruce A Knutson
    Department of Biochemistry, Purdue University, West Lafayatte, IN 47907, USA
    Virology 385:198-203. 2009
  7. ncbi request reprint An in vitro transcription system for studying vaccinia virus early genes
    Steven S Broyles
    Department of Biochemistry, Purdue University, West Lafayette, IN, USA
    Methods Mol Biol 269:135-42. 2004
  8. doi request reprint Expansion of poxvirus RNA polymerase subunits sharing homology with corresponding subunits of RNA polymerase II
    Bruce A Knutson
    Department of Biochemistry, Purdue University, West Lafayette, IN 47906, USA
    Virus Genes 36:307-11. 2008
  9. pmc Host cell nuclear proteins are recruited to cytoplasmic vaccinia virus replication complexes
    Jaewook Oh
    Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA
    J Virol 79:12852-60. 2005
  10. ncbi request reprint A natural vaccinia virus promoter with exceptional capacity to direct protein synthesis
    Xu Liu
    Department of Biochemistry, Purdue University, 175 South University Street, West Lafayette, IN 47907 1153, USA
    J Virol Methods 122:141-5. 2004

Research Grants

  1. TRAINING IN BIOCHEMISTRY AND MOLECULAR BIOLOGY
    STEVEN BROYLES; Fiscal Year: 2003

Collaborators

Detail Information

Publications10

  1. ncbi request reprint Vaccinia virus transcription
    Steven S Broyles
    Department of Biochemistry, Purdue University, West Lafayette, IN 47907 1153, USA
    J Gen Virol 84:2293-303. 2003
    ..Vaccinia virus resembles nuclear DNA viruses in the integration of viral and host proteins for viral mRNA synthesis, yet is markedly less reliant on host proteins than its nuclear counterparts...
  2. pmc Antiviral activity of distamycin A against vaccinia virus is the result of inhibition of postreplicative mRNA synthesis
    Steven S Broyles
    Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907 1153, USA
    J Virol 78:2137-41. 2004
    ....
  3. pmc The age of reverse biochemistry
    Steven S Broyles
    Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA
    Protein Sci 16:2-3. 2007
  4. pmc Vaccinia virus intermediate and late promoter elements are targeted by the TATA-binding protein
    Bruce A Knutson
    Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA
    J Virol 80:6784-93. 2006
    ....
  5. doi request reprint Downregulation of vaccinia virus intermediate and late promoters by host transcription factor YY1
    Bruce A Knutson
    Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA
    J Gen Virol 90:1592-9. 2009
    ..These results indicate that YY1 functions to negatively regulate these vaccinia virus promoters by binding to their initiator elements...
  6. doi request reprint Bidirectional transcriptional promoters in the vaccinia virus genome
    Bruce A Knutson
    Department of Biochemistry, Purdue University, West Lafayatte, IN 47907, USA
    Virology 385:198-203. 2009
    ..Nucleotide replacements in the initiator element of a bidirectional promoter lead to activation of the other, suggesting that bidirectional promoter arrangement is a mechanism of attenuating promoter strength...
  7. ncbi request reprint An in vitro transcription system for studying vaccinia virus early genes
    Steven S Broyles
    Department of Biochemistry, Purdue University, West Lafayette, IN, USA
    Methods Mol Biol 269:135-42. 2004
    ....
  8. doi request reprint Expansion of poxvirus RNA polymerase subunits sharing homology with corresponding subunits of RNA polymerase II
    Bruce A Knutson
    Department of Biochemistry, Purdue University, West Lafayette, IN 47906, USA
    Virus Genes 36:307-11. 2008
    ..These results strengthen the concept that poxviral RNA polymerases likely evolved from cellular RNA polymerases...
  9. pmc Host cell nuclear proteins are recruited to cytoplasmic vaccinia virus replication complexes
    Jaewook Oh
    Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA
    J Virol 79:12852-60. 2005
    ..These observations indicate that vaccinia virus replication complexes have ready access to nuclear proteins by allowing leakage from the nucleus...
  10. ncbi request reprint A natural vaccinia virus promoter with exceptional capacity to direct protein synthesis
    Xu Liu
    Department of Biochemistry, Purdue University, 175 South University Street, West Lafayette, IN 47907 1153, USA
    J Virol Methods 122:141-5. 2004
    ..The I1L promoter had high activity in both transient transfection experiments and in the context of recombinant viruses. The I1L promoter should be useful for high-level protein synthesis and poxvirus studies in general...

Research Grants1

  1. TRAINING IN BIOCHEMISTRY AND MOLECULAR BIOLOGY
    STEVEN BROYLES; Fiscal Year: 2003
    ..We feel that laboratory experience is an important indicator of motivation and persistence for conducting thesis research. Our students complete Ph.D. requirements in an average of 5 years after their arrival. ..