Research Topics
| Terry L RissSummaryAffiliation: Promega Corporation Country: USA Publications
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Detail Information
Publications
Use of multiple assay endpoints to investigate the effects of incubation time, dose of toxin, and plating density in cell-based cytotoxicity assaysTerry L Riss
Cellular Analysis, Promega Corporation, Madison, WI 53575, USA
Assay Drug Dev Technol 2:51-62. 2004..These results illustrate the importance of understanding the kinetics and mechanism of cell death of each in vitro model system as prerequisites for choosing the most appropriate assay method...
Cytotoxicity testing: measuring viable cells, dead cells, and detecting mechanism of cell deathTerry L Riss
Promega Corporation, Madison, WI, USA
Methods Mol Biol 740:103-14. 2011..Data analysis from the measurement of three marker protease activities from the same sample provides a useful tool to help uncover the mechanism of cell death and can serve as an internal control to help identify assay artifacts...
Cell-based bioluminescent assays for all three proteasome activities in a homogeneous formatRichard A Moravec
Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA
Anal Biochem 387:294-302. 2009..These cell-based proteasome assays are direct, simple, and sensitive, making them ideal for high-throughput screening...
Multiplex caspase activity and cytotoxicity assaysAndrew L Niles
Promega Corporation, Madison, WI, USA
Methods Mol Biol 414:151-62. 2008..Furthermore, multiplexing caspase activity assays with viability and/or cytotoxicity assays can support conclusions regarding cytotoxic mechanism and provide normalization that may help correct for differences in cell number...
A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markersAndrew L Niles
Promega Corp, Madison, WI 53711, USA
Anal Biochem 366:197-206. 2007..Ratiometric measurement of viable and dead cells in the same sample provides an internal control that can be used to normalize data from other cell-based assays...
Bioluminescent assays for ADMETJames J Cali
Promega Corp, 2800 Woods Hollow Road, Madison, WI 53711, USA
Expert Opin Drug Metab Toxicol 4:103-20. 2008..The bioluminescent ADMET assays are rapid and sensitive, amenable to automated high-throughput applications and offer significant advantages over alternative methods...
A bioluminescent assay for monoamine oxidase activityMichael P Valley
Promega Corporation, Madison, WI 53711, USA
Anal Biochem 359:238-46. 2006..Other advantages of this bioluminescent assay over comparable fluorescent assays are discussed...
New bioluminogenic substrates for monoamine oxidase assaysWenhui Zhou
Promega Biosciences, Inc, 277 Granada Drive, San Luis Obispo, California 93401, USA
J Am Chem Soc 128:3122-3. 2006..This design strategy should be applicable to fluorogenic MAO substrates and could broaden the structural requirements of substrates for other enzyme assays...
Caspase activity assaysAndrew L Niles
Promega Corporation, Madison, WI, USA
Methods Mol Biol 414:137-50. 2008..The highlighted techniques in this chapter are cost efficient and allow for the rapid exploration of thousands of combinations and conditions...
Homogeneous, bioluminescent proteasome assaysMartha A O'Brien
Promega Corporation, Madison, WI, USA
Methods Mol Biol 414:163-81. 2008..The bioluminescent, coupled-enzyme format enables sensitive and rapid protease assays ideal for inhibitor screening...
In vitro viability and cytotoxicity testing and same-well multi-parametric combinations for high throughput screeningAndrew L Niles
Research Department, Promega Corporation, 2800 Woods Hollow Road, Madison, WI, USA
Curr Chem Genomics 3:33-41. 2009..Lastly, we discuss the advantages of combining specific HTS compatible assays into multi-parametric, same-well formats...
