Andrew L Niles

Summary

Affiliation: Promega Corporation
Country: USA

Publications

  1. ncbi request reprint A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers
    Andrew L Niles
    Promega Corp, Madison, WI 53711, USA
    Anal Biochem 366:197-206. 2007
  2. pmc In vitro viability and cytotoxicity testing and same-well multi-parametric combinations for high throughput screening
    Andrew L Niles
    Research Department, Promega Corporation, 2800 Woods Hollow Road, Madison, WI, USA
    Curr Chem Genomics 3:33-41. 2009
  3. doi request reprint Update on in vitro cytotoxicity assays for drug development
    Andrew L Niles
    Senior Research Scientist Promega Corporation, Research and Development, 2800 Woods Hollow Road, Madison, Wisconsin, 53711, USA 1 608 247 4330, ext 1447 1 608 298 4818
    Expert Opin Drug Discov 3:655-69. 2008
  4. ncbi request reprint Multiplex caspase activity and cytotoxicity assays
    Andrew L Niles
    Promega Corporation, Madison, WI, USA
    Methods Mol Biol 414:151-62. 2008
  5. doi request reprint Cytotoxicity testing: measuring viable cells, dead cells, and detecting mechanism of cell death
    Terry L Riss
    Promega Corporation, Madison, WI, USA
    Methods Mol Biol 740:103-14. 2011
  6. ncbi request reprint Caspase activity assays
    Andrew L Niles
    Promega Corporation, Madison, WI, USA
    Methods Mol Biol 414:137-50. 2008

Detail Information

Publications6

  1. ncbi request reprint A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers
    Andrew L Niles
    Promega Corp, Madison, WI 53711, USA
    Anal Biochem 366:197-206. 2007
    ..Ratiometric measurement of viable and dead cells in the same sample provides an internal control that can be used to normalize data from other cell-based assays...
  2. pmc In vitro viability and cytotoxicity testing and same-well multi-parametric combinations for high throughput screening
    Andrew L Niles
    Research Department, Promega Corporation, 2800 Woods Hollow Road, Madison, WI, USA
    Curr Chem Genomics 3:33-41. 2009
    ..Lastly, we discuss the advantages of combining specific HTS compatible assays into multi-parametric, same-well formats...
  3. doi request reprint Update on in vitro cytotoxicity assays for drug development
    Andrew L Niles
    Senior Research Scientist Promega Corporation, Research and Development, 2800 Woods Hollow Road, Madison, Wisconsin, 53711, USA 1 608 247 4330, ext 1447 1 608 298 4818
    Expert Opin Drug Discov 3:655-69. 2008
    ..Results/conclusion: Each of these methods has its relative merits and detractions; however multi-parametric methods using both viability and cytotoxicity markers may mitigate the inherent shortcomings of single parameter measures...
  4. ncbi request reprint Multiplex caspase activity and cytotoxicity assays
    Andrew L Niles
    Promega Corporation, Madison, WI, USA
    Methods Mol Biol 414:151-62. 2008
    ..Furthermore, multiplexing caspase activity assays with viability and/or cytotoxicity assays can support conclusions regarding cytotoxic mechanism and provide normalization that may help correct for differences in cell number...
  5. doi request reprint Cytotoxicity testing: measuring viable cells, dead cells, and detecting mechanism of cell death
    Terry L Riss
    Promega Corporation, Madison, WI, USA
    Methods Mol Biol 740:103-14. 2011
    ..Data analysis from the measurement of three marker protease activities from the same sample provides a useful tool to help uncover the mechanism of cell death and can serve as an internal control to help identify assay artifacts...
  6. ncbi request reprint Caspase activity assays
    Andrew L Niles
    Promega Corporation, Madison, WI, USA
    Methods Mol Biol 414:137-50. 2008
    ..The highlighted techniques in this chapter are cost efficient and allow for the rapid exploration of thousands of combinations and conditions...