Nidhi Nath

Summary

Affiliation: Promega Corporation
Country: USA

Publications

  1. doi request reprint Homogeneous plate based antibody internalization assay using pH sensor fluorescent dye
    Nidhi Nath
    Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, United States Electronic address
    J Immunol Methods 431:11-21. 2016
  2. doi request reprint On-bead antibody-small molecule conjugation using high-capacity magnetic beads
    Nidhi Nath
    Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, United States Electronic address
    J Immunol Methods 426:95-103. 2015
  3. doi request reprint Improving protein array performance: focus on washing and storage conditions
    Nidhi Nath
    Research and Development, Promega Corporation, Madison, Wisconsin 53711, USA
    J Proteome Res 7:4475-82. 2008
  4. doi request reprint Protein-protein interaction studies on protein arrays: effect of detection strategies on signal-to-background ratios
    Robin Hurst
    Research and Development, Promega Corporation, Madison, WI 53711, USA
    Anal Biochem 392:45-53. 2009
  5. ncbi request reprint Fabrication of a reversible protein array directly from cell lysate using a stimuli-responsive polypeptide
    Nidhi Nath
    Department of Biomedical Engineering, Duke University, Box 90281, Durham, North Carolina 27708, USA
    Anal Chem 75:709-15. 2003
  6. ncbi request reprint Capture and release of proteins on the nanoscale by stimuli-responsive elastin-like polypeptide "switches"
    Jinho Hyun
    Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708 0281, USA
    J Am Chem Soc 126:7330-5. 2004
  7. ncbi request reprint Label free colorimetric biosensing using nanoparticles
    Nidhi Nath
    Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA
    J Fluoresc 14:377-89. 2004

Collaborators

  • Robin Hurst
  • Jinho Hyun
  • Michael R Slater
  • Brad Hook
  • Douglas R Storts
  • Jim Hartnett
  • Ashutosh Chilkoti
  • Stefan Zauscher
  • Woo Kyung Lee

Detail Information

Publications7

  1. doi request reprint Homogeneous plate based antibody internalization assay using pH sensor fluorescent dye
    Nidhi Nath
    Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, United States Electronic address
    J Immunol Methods 431:11-21. 2016
    ..We finally present two new approaches using the pHAb dye, which will be beneficial for screening a large number of antibody samples during early monoclonal development phase. ..
  2. doi request reprint On-bead antibody-small molecule conjugation using high-capacity magnetic beads
    Nidhi Nath
    Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, United States Electronic address
    J Immunol Methods 426:95-103. 2015
    ..This method enables simple and rapid processing of multiple samples in parallel with high-efficiency antibody recovery. It is further shown that recovered antibodies are functional and compatible with downstream applications. ..
  3. doi request reprint Improving protein array performance: focus on washing and storage conditions
    Nidhi Nath
    Research and Development, Promega Corporation, Madison, Wisconsin 53711, USA
    J Proteome Res 7:4475-82. 2008
    ..The findings indicate that for large ongoing studies, proteins of interest expressed in cell-free extract can be stored at -70 degrees C and repeatedly used to print small batches of protein array slides to be used over a few weeks...
  4. doi request reprint Protein-protein interaction studies on protein arrays: effect of detection strategies on signal-to-background ratios
    Robin Hurst
    Research and Development, Promega Corporation, Madison, WI 53711, USA
    Anal Biochem 392:45-53. 2009
    ..This simple method for creating protein arrays in combination with our analyses of several detection strategies should increase the usefulness of protein array technologies...
  5. ncbi request reprint Fabrication of a reversible protein array directly from cell lysate using a stimuli-responsive polypeptide
    Nidhi Nath
    Department of Biomedical Engineering, Duke University, Box 90281, Durham, North Carolina 27708, USA
    Anal Chem 75:709-15. 2003
    ..TRAP has application in lab-on-a-chip bioanalytical devices as well as in the fabrication of peptide and protein arrays...
  6. ncbi request reprint Capture and release of proteins on the nanoscale by stimuli-responsive elastin-like polypeptide "switches"
    Jinho Hyun
    Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708 0281, USA
    J Am Chem Soc 126:7330-5. 2004
    ..We also investigated the molecular mechanism underlying this switch by measuring the height changes that accompany the binding and desorption steps and by adhesion force spectroscopy using atomic force microscopy...
  7. ncbi request reprint Label free colorimetric biosensing using nanoparticles
    Nidhi Nath
    Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA
    J Fluoresc 14:377-89. 2004
    ..We conclude by presenting the future prospects and challenges for the successful transition of this technology from the laboratory to a commercial product...