Frank Fan

Summary

Affiliation: Promega Corporation
Country: USA

Publications

  1. pmc Improved dual-luciferase reporter assays for nuclear receptors
    Aileen Paguio
    Promega Corporation, Madison, Wisconsin, USA
    Curr Chem Genomics 4:43-9. 2010
  2. pmc Luciferase Reporter Assay System for Deciphering GPCR Pathways
    Zhijie Cheng
    Department of Research, Promega Corporation, Madison, WI53711, USA
    Curr Chem Genomics 4:84-91. 2010
  3. pmc A novel bioluminescent protease assay using engineered firefly luciferase
    Susan S Wigdal
    Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA
    Curr Chem Genomics 2:16-28. 2008
  4. ncbi request reprint Bioluminescent assays for high-throughput screening
    Frank Fan
    Promega Corporation, Madison, WI 53711, USA
    Assay Drug Dev Technol 5:127-36. 2007
  5. pmc Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate
    Mary P Hall
    Promega Corporation, Madison, Wisconsin 53711, United States
    ACS Chem Biol 7:1848-57. 2012
  6. doi request reprint Luminescent biosensors for real-time monitoring of intracellular cAMP
    Brock F Binkowski
    Promega Corporation, Madison, WI, USA
    Methods Mol Biol 756:263-71. 2011
  7. doi request reprint A luminescent assay for real-time measurements of receptor endocytosis in living cells
    Matthew B Robers
    Promega Corporation, Fitchburg, WI 53711, USA Electronic address
    Anal Biochem 489:1-8. 2015
  8. doi request reprint Development of a robust reporter-based ADCC assay with frozen, thaw-and-use cells to measure Fc effector function of therapeutic antibodies
    Zhijie Jey Cheng
    Department of Research, Promega Corporation, Madison, WI 53711, United States Electronic address
    J Immunol Methods 414:69-81. 2014
  9. doi request reprint Engineered luciferases for molecular sensing in living cells
    Brock Binkowski
    Promega Corporation, Madison, WI 53711, USA
    Curr Opin Biotechnol 20:14-8. 2009
  10. ncbi request reprint A luminescent biosensor with increased dynamic range for intracellular cAMP
    Brock F Binkowski
    Promega Corporation, 2800 Woods Hollow Rd, Madison, Wisconsin 53711, United States
    ACS Chem Biol 6:1193-7. 2011

Collaborators

  • Brock F Binkowski
  • Michael P Valley
  • Thomas Machleidt
  • Keith V Wood
  • Aileen Paguio
  • Matthew B Robers
  • Zhijie Jey Cheng
  • Christopher T Eggers
  • Mary P Hall
  • Denise Garvin
  • Pete Stecha
  • Zhijie Cheng
  • Susan S Wigdal
  • George Otto
  • Chad Zimprich
  • Cesear Corona
  • Mei Cong
  • Jim Hartnett
  • Mark McDougall
  • Richard Moravec
  • Teresa Surowy
  • Laurie Engel
  • Michael R Slater
  • Dieter H Klaubert
  • Monika G Wood
  • Poncho L Meisenheimer
  • Paul Otto
  • Braeden L Butler
  • Gediminas Vidugiris
  • James Unch
  • Lance P Encell
  • Hélène A Benink
  • Kristopher Zimmerman
  • Keith Wood
  • Jessica L Anderson
  • Gediminas J Vidugiris
  • John Shultz

Detail Information

Publications11

  1. pmc Improved dual-luciferase reporter assays for nuclear receptors
    Aileen Paguio
    Promega Corporation, Madison, Wisconsin, USA
    Curr Chem Genomics 4:43-9. 2010
    ..These luciferase reporter assays provided better research and drug discovery tools for studying the functions of full length nuclear receptors and ligand binding domains...
  2. pmc Luciferase Reporter Assay System for Deciphering GPCR Pathways
    Zhijie Cheng
    Department of Research, Promega Corporation, Madison, WI53711, USA
    Curr Chem Genomics 4:84-91. 2010
    ..Furthermore, we demonstrated that these assays can be readily used for potency rankings of agonists and antagonists, and for high throughput screening...
  3. pmc A novel bioluminescent protease assay using engineered firefly luciferase
    Susan S Wigdal
    Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA
    Curr Chem Genomics 2:16-28. 2008
    ....
  4. ncbi request reprint Bioluminescent assays for high-throughput screening
    Frank Fan
    Promega Corporation, Madison, WI 53711, USA
    Assay Drug Dev Technol 5:127-36. 2007
    ..Finally, new methods for modulating the specific activity of luciferases are leading to the development of intracellular biosensors for dynamic detection of physiological processes...
  5. pmc Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate
    Mary P Hall
    Promega Corporation, Madison, Wisconsin 53711, United States
    ACS Chem Biol 7:1848-57. 2012
    ..Reporter quantitation is achievable even at very low expression levels to facilitate more reliable coupling with endogenous cellular processes...
  6. doi request reprint Luminescent biosensors for real-time monitoring of intracellular cAMP
    Brock F Binkowski
    Promega Corporation, Madison, WI, USA
    Methods Mol Biol 756:263-71. 2011
    ..Here we present the use of genetically encoded, luminescent biosensors that allow a facile, non-lytic assay format for monitoring cAMP dynamics in living cells...
  7. doi request reprint A luminescent assay for real-time measurements of receptor endocytosis in living cells
    Matthew B Robers
    Promega Corporation, Fitchburg, WI 53711, USA Electronic address
    Anal Biochem 489:1-8. 2015
    ..This article also describes the application of this technology to endocytosis of epidermal growth factor receptor (EGFR), demonstrating potential applicability of the method beyond GPCRs. ..
  8. doi request reprint Development of a robust reporter-based ADCC assay with frozen, thaw-and-use cells to measure Fc effector function of therapeutic antibodies
    Zhijie Jey Cheng
    Department of Research, Promega Corporation, Madison, WI 53711, United States Electronic address
    J Immunol Methods 414:69-81. 2014
    ..Together these data demonstrate that this robust ADCC reporter assay meets the requirement of a potency bioassay that can quantify antibody Fc effector function in ADCC mechanism of action during drug discovery and development. ..
  9. doi request reprint Engineered luciferases for molecular sensing in living cells
    Brock Binkowski
    Promega Corporation, Madison, WI 53711, USA
    Curr Opin Biotechnol 20:14-8. 2009
    ..Building on successes achieved in cell culture, these sensors are now beginning to reveal molecular processes within living organisms...
  10. ncbi request reprint A luminescent biosensor with increased dynamic range for intracellular cAMP
    Brock F Binkowski
    Promega Corporation, 2800 Woods Hollow Rd, Madison, Wisconsin 53711, United States
    ACS Chem Biol 6:1193-7. 2011
    ..We demonstrate the utility of this construct to detect a broad range of receptor activity, together with showing suitability for use in high-throughput screening...
  11. doi request reprint Novel genetically encoded biosensors using firefly luciferase
    Frank Fan
    ACS Chem Biol 3:346-51. 2008
    ..These and other luminescent biosensors will be useful for the sensitive detection of cellular physiology in research and drug discovery...