THOMAS SILHAVY

Summary

Affiliation: Princeton University
Country: USA

Publications

  1. ncbi request reprint Imp/OstA is required for cell envelope biogenesis in Escherichia coli
    Martin Braun
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Mol Microbiol 45:1289-302. 2002
  2. pmc BamE modulates the Escherichia coli beta-barrel assembly machine component BamA
    Nathan W Rigel
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA
    J Bacteriol 194:1002-8. 2012
  3. pmc The bacterial cell envelope
    Thomas J Silhavy
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Cold Spring Harb Perspect Biol 2:a000414. 2010
  4. pmc An ABC transport system that maintains lipid asymmetry in the gram-negative outer membrane
    Juliana C Malinverni
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 106:8009-14. 2009
  5. ncbi request reprint Probing the barrier function of the outer membrane with chemical conditionality
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    ACS Chem Biol 1:385-95. 2006
  6. pmc Identification of two inner-membrane proteins required for the transport of lipopolysaccharide to the outer membrane of Escherichia coli
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 105:5537-42. 2008
  7. pmc Nonconsecutive disulfide bond formation in an essential integral outer membrane protein
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 107:12245-50. 2010
  8. ncbi request reprint Chemical conditionality: a genetic strategy to probe organelle assembly
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Cell 121:307-17. 2005
  9. ncbi request reprint YfiO stabilizes the YaeT complex and is essential for outer membrane protein assembly in Escherichia coli
    Juliana C Malinverni
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Mol Microbiol 61:151-64. 2006
  10. pmc The extracytoplasmic adaptor protein CpxP is degraded with substrate by DegP
    Daniel D Isaac
    Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, Washington Road, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 102:17775-9. 2005

Research Grants

  1. REGULATION OF THE MAJOR OUTER MEMBRANE PORIN PROTEINS
    THOMAS SILHAVY; Fiscal Year: 2001
  2. GENETIC ANALYSIS OF PROTEIN EXPORT
    Thomas J Silhavy; Fiscal Year: 2010
  3. Regulation of Stationary Phase in Escherichia coli
    THOMAS SILHAVY; Fiscal Year: 2006
  4. Regulation of Stationary Phase in Escherichia coli
    THOMAS SILHAVY; Fiscal Year: 2007
  5. GENETIC ANALYSIS OF PROTEIN EXPORT
    THOMAS SILHAVY; Fiscal Year: 2007
  6. Regulation of Stationary Phase in Escherichia coli
    THOMAS SILHAVY; Fiscal Year: 2009
  7. Regulation of Stationary Phase in Escherichia coli
    Thomas J Silhavy; Fiscal Year: 2010
  8. REGULATION OF THE MAJOR OUTER MEMBRANE PORIN PROTEINS
    THOMAS SILHAVY; Fiscal Year: 1993
  9. GENETIC ANALYSIS OF PROTEIN EXPORT
    THOMAS SILHAVY; Fiscal Year: 1990
  10. GENETIC ANALYSIS OF PROTEIN EXPORT
    Thomas J Silhavy; Fiscal Year: 2010

Collaborators

  • Robert G Endres
  • R Misra
  • Karen Otto
  • Dominique Belin
  • Richard Gourse
  • Andrei N Lupas
  • T Nystrom
  • Sheryl S Justice
  • A Molinaro
  • Natividad Ruiz
  • Celeste N Peterson
  • Juliana C Malinverni
  • Daniel Kahne
  • Tao Wu
  • Valerie J Carabetta
  • Joseph G Sklar
  • Seokhee Kim
  • Mark J Mandel
  • Nathan W Rigel
  • Daniel D Isaac
  • Nicholas J Hand
  • Patricia A DiGiuseppe
  • Johna van Stelten
  • Paola Sperandeo
  • Julie E Button
  • Asa Fredriksson
  • Alejandro R Ureta
  • Oliver Schmidt
  • Luisa S Gronenberg
  • Tamas Gaal
  • Scott J Hultgren
  • Karine A Gibbs
  • Yvonne M Lee
  • Amy R Duguay
  • Eric Batchelor
  • Howard A Shuman
  • Christina Wilson Bowers
  • Martin Braun
  • Jaclyn Schwalm
  • Dante P Ricci
  • Ileana M Cristea
  • Filo Silva
  • Sidney R Kushner
  • Bijoy K Mohanty
  • Andrea Carpentieri
  • Cristina De Castro
  • Alessandra Polissi
  • Fion K Lau
  • Gianni Dehò
  • Verena J Schuenemann
  • Manuel Ballesteros
  • Orjan Persson
  • Sergej Djuranovic
  • Ned S Wingreen
  • Piotr Sliz
  • JORG MARTIN
  • Stephen C Harrison
  • Shu Sin Chng
  • Andrew C McCandlish
  • Tahmeena Chowdhury
  • John Werner
  • Jerome S Pinkner
  • Juliana Malinverni
  • Mark Goulian
  • Jun Xu
  • Julie A Theriot
  • Roger W Hendrix
  • Fion Lau

Detail Information

Publications42

  1. ncbi request reprint Imp/OstA is required for cell envelope biogenesis in Escherichia coli
    Martin Braun
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Mol Microbiol 45:1289-302. 2002
    ..Upon depletion of Imp, lipids and outer membrane proteins appear in a novel membrane fraction with higher density than the outer membrane. We propose Imp to be part of a targeting/usher system for components of the outer membrane...
  2. pmc BamE modulates the Escherichia coli beta-barrel assembly machine component BamA
    Nathan W Rigel
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA
    J Bacteriol 194:1002-8. 2012
    ..Strikingly, when ΔbamE mutant cells are treated with proteinase K, BamA is degraded beyond detection. Taken together, our findings suggest that BamE modulates the conformation of BamA, likely through its interactions with BamD...
  3. pmc The bacterial cell envelope
    Thomas J Silhavy
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Cold Spring Harb Perspect Biol 2:a000414. 2010
    ..Threading through these layers of peptidoglycan are long anionic polymers, called teichoic acids. The composition and organization of these envelope layers and recent insights into the mechanisms of cell envelope assembly are discussed...
  4. pmc An ABC transport system that maintains lipid asymmetry in the gram-negative outer membrane
    Juliana C Malinverni
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 106:8009-14. 2009
    ..We propose that the Mla pathway constitutes a bacterial intermembrane PL trafficking system...
  5. ncbi request reprint Probing the barrier function of the outer membrane with chemical conditionality
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    ACS Chem Biol 1:385-95. 2006
    ..The remarkable chemical specificity of the imp4213 suppressors provides insights into the molecular nature of the OM permeability barrier...
  6. pmc Identification of two inner-membrane proteins required for the transport of lipopolysaccharide to the outer membrane of Escherichia coli
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 105:5537-42. 2008
    ..We propose that these two proteins, which we have renamed LptF and LptG, respectively, are the missing transmembrane components of the ABC transporter that, together with LptB, functions to extract LPS from the IM en route to the OM...
  7. pmc Nonconsecutive disulfide bond formation in an essential integral outer membrane protein
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 107:12245-50. 2010
    ..Thus, oxidation is completed only after LptD interacts with LptE, an interaction that occurs at the outer membrane and seems necessary for LptD folding...
  8. ncbi request reprint Chemical conditionality: a genetic strategy to probe organelle assembly
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Cell 121:307-17. 2005
    ..We posit that panels of toxic small molecules will be useful for generating chemical conditionalities that enable identification of genes required for organelle assembly in other organisms...
  9. ncbi request reprint YfiO stabilizes the YaeT complex and is essential for outer membrane protein assembly in Escherichia coli
    Juliana C Malinverni
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Mol Microbiol 61:151-64. 2006
    ..Genetic analysis verifies the importance of YfiO and its interactions with NlpB in maintaining the functional integrity of the YaeT complex...
  10. pmc The extracytoplasmic adaptor protein CpxP is degraded with substrate by DegP
    Daniel D Isaac
    Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, Washington Road, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 102:17775-9. 2005
    ..Our data suggest that CpxP functions as a periplasmic adaptor protein that is required for the effective proteolysis of a subset of misfolded substrates by the DegP protease...
  11. ncbi request reprint Advances in understanding bacterial outer-membrane biogenesis
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Nat Rev Microbiol 4:57-66. 2006
    ..Here, we review the new approaches that have led to the recent discovery of components of the machinery involved in the biogenesis of this distinctive cellular organelle...
  12. pmc LrhA regulates rpoS translation in response to the Rcs phosphorelay system in Escherichia coli
    Celeste N Peterson
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    J Bacteriol 188:3175-81. 2006
    ..In addition, mutations in the gene encoding the DNA motor protein FtsK induce lrhA synthesis, which may explain why such strains fail to accumulate RpoS in stationary phase...
  13. ncbi request reprint Identification of a multicomponent complex required for outer membrane biogenesis in Escherichia coli
    Tao Wu
    Department of Chemistry, Princeton University, Princeton, New Jersey 08544, USA
    Cell 121:235-45. 2005
    ..These genetic interactions suggest a role for YfgL, one of the lipoprotein components of the protein assembly complex, in a homeostatic control mechanism that coordinates the overall OM assembly process...
  14. ncbi request reprint Sensing external stress: watchdogs of the Escherichia coli cell envelope
    Natividad Ruiz
    Molecular Biology Department, Princeton University, Princeton, NJ 08544, USA
    Curr Opin Microbiol 8:122-6. 2005
    ..In this review, we discuss the recent progress in our understanding of the Cpx and sigmaE systems and compare how both function to maintain the integrity of the cell envelope...
  15. pmc Surface sensing and adhesion of Escherichia coli controlled by the Cpx-signaling pathway
    Karen Otto
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 99:2287-92. 2002
    ....
  16. pmc Signal detection and target gene induction by the CpxRA two-component system
    Patricia A DiGiuseppe
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    J Bacteriol 185:2432-40. 2003
    ..Finally, we show that two Cpx-regulated genes are differentially upregulated in response to different envelope stresses, suggesting the existence of three stress-responsive systems...
  17. pmc Effects of antibiotics and a proto-oncogene homolog on destruction of protein translocator SecY
    Johna van Stelten
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Science 325:753-6. 2009
    ..Intriguingly, YccA is a functional homolog of the proto-oncogene product Bax Inhibitor-1, which may share a similar mechanism of action in regulating apoptosis upon prolonged secretion stress...
  18. pmc Kinetic analysis of the assembly of the outer membrane protein LamB in Escherichia coli mutants each lacking a secretion or targeting factor in a different cellular compartment
    Alejandro R Ureta
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    J Bacteriol 189:446-54. 2007
    ....
  19. pmc Transport of lipopolysaccharide across the cell envelope: the long road of discovery
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Nat Rev Microbiol 7:677-83. 2009
    ....
  20. ncbi request reprint Quality control in the bacterial periplasm
    Amy R Duguay
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544 1014, USA
    Biochim Biophys Acta 1694:121-34. 2004
    ..coli. In addition, we discuss the apparent redundancy of the folding factors in the periplasm, and we consider the potential importance of the functional overlap that exists...
  21. pmc The response regulator SprE (RssB) modulates polyadenylation and mRNA stability in Escherichia coli
    Valerie J Carabetta
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    J Bacteriol 191:6812-21. 2009
    ....
  22. pmc Constitutive activation of the Escherichia coli Pho regulon upregulates rpoS translation in an Hfq-dependent fashion
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    J Bacteriol 185:5984-92. 2003
    ..This upregulation of rpoS translation is Hfq dependent, suggesting the involvement of a small regulatory RNA (sRNA). The transcription of this yet-to-be-identified sRNA is regulated by the PhoBR two-component system...
  23. pmc Defining the roles of the periplasmic chaperones SurA, Skp, and DegP in Escherichia coli
    Joseph G Sklar
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Genes Dev 21:2473-84. 2007
    ..The seemingly redundant periplasmic chaperones do function in parallel, but the relative importance of the primary function of each pathway depends on whether or not cells are under stress...
  24. pmc Lipoprotein SmpA is a component of the YaeT complex that assembles outer membrane proteins in Escherichia coli
    Joseph G Sklar
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 104:6400-5. 2007
    ..Biochemical experiments show that SmpA is involved in maintaining complex stability. Taken together, these experiments establish an important role for SmpA in both the structure and function of the YaeT complex...
  25. pmc The response regulator SprE (RssB) is required for maintaining poly(A) polymerase I-degradosome association during stationary phase
    Valerie J Carabetta
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    J Bacteriol 192:3713-21. 2010
    ..Moreover, we demonstrate that the majority of these PAP I interactions are formed via protein-protein interactions and that SprE plays an important role in the maintenance of the PAP I-degradosome association during stationary phase...
  26. pmc RpoS proteolysis is regulated by a mechanism that does not require the SprE (RssB) response regulator phosphorylation site
    Celeste N Peterson
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    J Bacteriol 186:7403-10. 2004
    ..Furthermore, our results indicate that phosphorylation of wild-type SprE occurs by a mechanism that is independent of acetyl phosphate...
  27. pmc Starvation for different nutrients in Escherichia coli results in differential modulation of RpoS levels and stability
    Mark J Mandel
    Department of Molecular Biology, 310 Lewis Thomas Laboratory, Princeton University, Princeton, NJ 08544, USA
    J Bacteriol 187:434-42. 2005
    ....
  28. pmc A suppressor of cell death caused by the loss of sigmaE downregulates extracytoplasmic stress responses and outer membrane vesicle production in Escherichia coli
    Julie E Button
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    J Bacteriol 189:1523-30. 2007
    ..We present a model in which sigma(E) is not essential per se but, rather, we propose that rpoE mutant cells die, possibly because they overreact to the absence of this sigma factor by triggering a cell death signal...
  29. pmc Null mutations in a Nudix gene, ygdP, implicate an alarmone response in a novel suppression of hybrid jamming
    Nicholas J Hand
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    J Bacteriol 185:6530-9. 2003
    ..Using an epitope-tagged ygdP construct, we present evidence that this response is sensitive to secretion-related stress and is regulated by differential proteolysis of YgdP in a self-limiting manner...
  30. pmc Secretion of LamB-LacZ by the signal recognition particle pathway of Escherichia coli
    Christina Wilson Bowers
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    J Bacteriol 185:5697-705. 2003
    ..Collectively, our results provide compelling in vivo evidence that there is an SRP-dependent cotranslational targeting mechanism in E. coli and argue against a role for trigger factor in pathway discrimination...
  31. pmc Escherichia coli starvation diets: essential nutrients weigh in distinctly
    Celeste N Peterson
    Department of Molecular Biology, Princeton University, NJ 08544, USA
    J Bacteriol 187:7549-53. 2005
  32. ncbi request reprint Structure and function of an essential component of the outer membrane protein assembly machine
    Seokhee Kim
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Science 317:961-4. 2007
    ..Analysis of POTRA domain deletions shows which are essential and provides a view of the spatial organization of this assembly machine...
  33. pmc Identification of a protein complex that assembles lipopolysaccharide in the outer membrane of Escherichia coli
    Tao Wu
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 103:11754-9. 2006
    ..We show that RlpB is also essential for cell viability and that the Imp/RlpB complex is responsible for LPS reaching the outer surface of the outer membrane...
  34. pmc Periplasmic peptidyl prolyl cis-trans isomerases are not essential for viability, but SurA is required for pilus biogenesis in Escherichia coli
    Sheryl S Justice
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Bacteriol 187:7680-6. 2005
    ....
  35. ncbi request reprint Complex spatial distribution and dynamics of an abundant Escherichia coli outer membrane protein, LamB
    Karine A Gibbs
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Mol Microbiol 53:1771-83. 2004
    ..In this study, we have demonstrated that LamB moves along the bacterial surface and that these movements are restricted by an underlying dynamic spiral pattern...
  36. pmc Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli
    Paola Sperandeo
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Piazza della Scienza 2, 20126 Milan, Italy
    J Bacteriol 190:4460-9. 2008
    ..Moreover, the location of at least one of these five proteins in every cellular compartment suggests a model for how the LPS assembly pathway is organized and ordered in space...
  37. pmc P pilus assembly motif necessary for activation of the CpxRA pathway by PapE in Escherichia coli
    Yvonne M Lee
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Bacteriol 186:4326-37. 2004
    ..Thus, the data presented in this study argue that PapE has features inherent in its structure or during its folding that act as specific inducers of Cpx signal transduction...
  38. pmc Continuous control in bacterial regulatory circuits
    Eric Batchelor
    Department of Physics, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Bacteriol 186:7618-25. 2004
    ..This mode of control is particularly interesting for an organism such as E. coli, which proliferates in diverse environments, and raises important questions regarding the biologically relevant inputs and outputs for this system...
  39. pmc Crl facilitates RNA polymerase holoenzyme formation
    Tamas Gaal
    Department of Bacteriology, University of Wisconsin Madison, 420 Henry Mall, Madison, WI 53706, USA
    J Bacteriol 188:7966-70. 2006
    ..Our results suggest that Crl facilitates holoenzyme formation, the first positive regulator identified with this mechanism of action...
  40. pmc prlF and yhaV encode a new toxin-antitoxin system in Escherichia coli
    Oliver Schmidt
    Department of Protein Evolution, Max Planck Institute for Developmental Biology, 72076, Tubingen, Germany
    J Mol Biol 372:894-905. 2007
    ..As homologs of MazE and RelE, respectively, PrlF and YhaV provide an evolutionary connection between the two best-characterized toxin-antitoxin systems in E. coli, mazEF and relEB...
  41. pmc Decline in ribosomal fidelity contributes to the accumulation and stabilization of the master stress response regulator sigmaS upon carbon starvation
    Asa Fredriksson
    Department of Cell and Molecular Biology Microbiology, Goteborg University, 405 30 Goteborg, Sweden
    Genes Dev 21:862-74. 2007
    ..We present a model for the sequence of events leading to the accumulation and activation of sigma(S) upon carbon starvation, which are linked to alterations in both ribosomal fidelity and efficiency...
  42. ncbi request reprint The art and design of genetic screens: Escherichia coli
    Howard A Shuman
    Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York 10032, USA
    Nat Rev Genet 4:419-31. 2003
    ..The diversity of biological processes that can be studied with lac genetics is remarkable and includes DNA metabolism, gene regulation and signal transduction, protein localization and folding, and even electron transport...

Research Grants31

  1. REGULATION OF THE MAJOR OUTER MEMBRANE PORIN PROTEINS
    THOMAS SILHAVY; Fiscal Year: 2001
    ..We have discovered two novel proteins, SprE and Crl, that function to control the stability and activity of RpoS respectively. Genetic and biochemical experiments are proposed to elucidate these novel regulatory mechanisms. ..
  2. GENETIC ANALYSIS OF PROTEIN EXPORT
    Thomas J Silhavy; Fiscal Year: 2010
    ..Such knowledge would facilitate the development of new antibiotics that would combat the growing problem of antibiotic resistance in this important class of pathogens. ..
  3. Regulation of Stationary Phase in Escherichia coli
    THOMAS SILHAVY; Fiscal Year: 2006
    ..abstract_text> ..
  4. Regulation of Stationary Phase in Escherichia coli
    THOMAS SILHAVY; Fiscal Year: 2007
    ..Thus insights gained in bacteria may shed light on similar processes in eukaryotic microbes, and perhaps animal cells as well. ..
  5. GENETIC ANALYSIS OF PROTEIN EXPORT
    THOMAS SILHAVY; Fiscal Year: 2007
    ..Results o: these studies may suggest new ways to treat or prevent certain types of bacterial infections. ..
  6. Regulation of Stationary Phase in Escherichia coli
    THOMAS SILHAVY; Fiscal Year: 2009
    ..Thus insights gained in bacteria may shed light on similar processes in eukaryotic microbes, and perhaps animal cells as well. ..
  7. Regulation of Stationary Phase in Escherichia coli
    Thomas J Silhavy; Fiscal Year: 2010
    ..Thus insights gained in bacteria may shed light on similar processes in eukaryotic microbes, and perhaps animal cells as well. ..
  8. REGULATION OF THE MAJOR OUTER MEMBRANE PORIN PROTEINS
    THOMAS SILHAVY; Fiscal Year: 1993
    ..The goal of this analysis is to gain insights into the sensory functions of EnvZ and how it acts mechanistically as a signal transducer to throw the genetic switch...
  9. GENETIC ANALYSIS OF PROTEIN EXPORT
    THOMAS SILHAVY; Fiscal Year: 1990
    ..In addition, we outline an approach to identifying mutants specifically defective in outer membrane targeting...
  10. GENETIC ANALYSIS OF PROTEIN EXPORT
    Thomas J Silhavy; Fiscal Year: 2010
    ..Such knowledge would facilitate the development of new antibiotics that would combat the growing problem of antibiotic resistance in this important class of pathogens. ..