Jay Shendure

Summary

Affiliation: Princeton University
Country: USA

Publications

  1. ncbi request reprint Accurate multiplex polony sequencing of an evolved bacterial genome
    Jay Shendure
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 309:1728-32. 2005
  2. ncbi request reprint Multiplex amplification of large sets of human exons
    Gregory J Porreca
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Nat Methods 4:931-6. 2007
  3. ncbi request reprint Long-range polony haplotyping of individual human chromosome molecules
    Kun Zhang
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Genet 38:382-7. 2006
  4. pmc Discovering functional transcription-factor combinations in the human cell cycle
    Zhou Zhu
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genome Res 15:848-55. 2005
  5. ncbi request reprint Single molecule profiling of alternative pre-mRNA splicing
    Jun Zhu
    Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Science 301:836-8. 2003
  6. ncbi request reprint Advanced sequencing technologies: methods and goals
    Jay Shendure
    Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Nat Rev Genet 5:335-44. 2004
  7. ncbi request reprint Fluorescent in situ sequencing on polymerase colonies
    Robi D Mitra
    Lipper Center for Computational Genetics, Harvard Medical School, 200 Longwood Ave, Boston, MA 02115, USA
    Anal Biochem 320:55-65. 2003
  8. pmc Computational discovery of sense-antisense transcription in the human and mouse genomes
    Jay Shendure
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Genome Biol 3:RESEARCH0044. 2002
  9. doi request reprint Polony DNA sequencing
    Gregory J Porreca
    Harvard Medical School, Boston, Massachusetts, USA
    Curr Protoc Mol Biol . 2006
  10. pmc Digital genotyping and haplotyping with polymerase colonies
    Robi D Mitra
    Lipper Center for Computational Genetics and Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 100:5926-31. 2003

Collaborators

Detail Information

Publications16

  1. ncbi request reprint Accurate multiplex polony sequencing of an evolved bacterial genome
    Jay Shendure
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 309:1728-32. 2005
    ..Cost per base was roughly one-ninth as much as that of conventional sequencing. Our protocols were implemented with off-the-shelf instrumentation and reagents...
  2. ncbi request reprint Multiplex amplification of large sets of human exons
    Gregory J Porreca
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Nat Methods 4:931-6. 2007
    ..We anticipate that highly multiplexed methods for targeted amplification will enable the comprehensive resequencing of human exons at a fraction of the cost of whole-genome resequencing...
  3. ncbi request reprint Long-range polony haplotyping of individual human chromosome molecules
    Kun Zhang
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Genet 38:382-7. 2006
    ..This haplotyping method is well suited for candidate gene-based association studies as well as for investigating the pattern of recombination in mammalian cells...
  4. pmc Discovering functional transcription-factor combinations in the human cell cycle
    Zhou Zhu
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genome Res 15:848-55. 2005
    ..We also detected many homotypic combinations, supporting the importance of binding-site density in transcriptional regulation of higher eukaryotes...
  5. ncbi request reprint Single molecule profiling of alternative pre-mRNA splicing
    Jun Zhu
    Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Science 301:836-8. 2003
    ..Digital polony exon profiling can be used to investigate the physiological and pathological roles of alternately spliced messenger RNAs, as well as the mechanisms by which these messenger RNAs are produced...
  6. ncbi request reprint Advanced sequencing technologies: methods and goals
    Jay Shendure
    Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Nat Rev Genet 5:335-44. 2004
  7. ncbi request reprint Fluorescent in situ sequencing on polymerase colonies
    Robi D Mitra
    Lipper Center for Computational Genetics, Harvard Medical School, 200 Longwood Ave, Boston, MA 02115, USA
    Anal Biochem 320:55-65. 2003
    ..Finally, we have developed software for automated image alignment and sequence calling...
  8. pmc Computational discovery of sense-antisense transcription in the human and mouse genomes
    Jay Shendure
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Genome Biol 3:RESEARCH0044. 2002
    ..We computationally mined public mouse and human expressed sequence tag (EST) databases to search for additional examples of bidirectionally transcribed genomic regions...
  9. doi request reprint Polony DNA sequencing
    Gregory J Porreca
    Harvard Medical School, Boston, Massachusetts, USA
    Curr Protoc Mol Biol . 2006
    ..Each sequencing run results in millions of 26-bp reads that can be aligned to the reference genome, allowing the identification of differences between sequences...
  10. pmc Digital genotyping and haplotyping with polymerase colonies
    Robi D Mitra
    Lipper Center for Computational Genetics and Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 100:5926-31. 2003
    ..The results indicate that polony genotyping and haplotyping may play an important role in understanding the structure of genetic variation...
  11. ncbi request reprint Identification of foreign gene sequences by transcript filtering against the human genome
    Griffin Weber
    Department of Adult Oncology, Dana Farber Cancer Institute, 44 Binney Street, Boston, Massachusetts 02115, USA
    Nat Genet 30:141-2. 2002
    ..We demonstrate the potential of this method by identifying sequences from known pathogens in established expressed-sequence tag libraries...
  12. pmc Characterization of apparently balanced chromosomal rearrangements from the developmental genome anatomy project
    Anne W Higgins
    Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Am J Hum Genet 82:712-22. 2008
    ..Chromosomal rearrangements, both balanced and unbalanced, in individuals with multiple congenital anomalies continue to be a valuable resource for gene discovery and annotation...
  13. ncbi request reprint A molecular pathway including Id2, Tbx5, and Nkx2-5 required for cardiac conduction system development
    Ivan P G Moskowitz
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Cell 129:1365-76. 2007
    ..We conclude that a molecular pathway including Tbx5, Nkx2-5, and Id2 coordinates specification of ventricular myocytes into the ventricular conduction system lineage...
  14. pmc Assaying chromosomal inversions by single-molecule haplotyping
    Daniel J Turner
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
    Nat Methods 3:439-45. 2006
    ..The generality of our methods to survey for, and genotype chromosomal inversions should help our understanding of the contribution of inversions to genomic variation, inherited diseases and cancer...
  15. ncbi request reprint Sequencing thoroughbreds
    George Church
    Nat Biotechnol 24:139. 2006
  16. ncbi request reprint The beginning of the end for microarrays?
    Jay Shendure
    Nat Methods 5:585-7. 2008