Joshua W Shaevitz

Summary

Affiliation: Princeton University
Country: USA

Publications

  1. pmc Efficient multiple object tracking using mutually repulsive active membranes
    Yi Deng
    Department of Physics, Princeton University, Princeton, New Jersey, United States of America
    PLoS ONE 8:e65769. 2013
  2. pmc Fast, multiphase volume adaptation to hyperosmotic shock by Escherichia coli
    Teuta Pilizota
    Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, United States of America
    PLoS ONE 7:e35205. 2012
  3. pmc Curvature and torsion in growing actin networks
    Joshua W Shaevitz
    Department of Integrative Biology, University of California, Berkeley, Berkeley, CA, USA
    Phys Biol 5:026006. 2008
  4. pmc Load fluctuations drive actin network growth
    Joshua W Shaevitz
    Department of Integrative Biology, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 104:15688-92. 2007
  5. ncbi request reprint Enhanced three-dimensional deconvolution microscopy using a measured depth-varying point-spread function
    Joshua W Shaevitz
    Department of Integrative Biology, University of California, Berkeley 94720, USA
    J Opt Soc Am A Opt Image Sci Vis 24:2622-7. 2007
  6. pmc Helical insertion of peptidoglycan produces chiral ordering of the bacterial cell wall
    Siyuan Wang
    Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 109:E595-604. 2012
  7. pmc The bacterial actin MreB rotates, and rotation depends on cell-wall assembly
    Sven van Teeffelen
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 108:15822-7. 2011
  8. ncbi request reprint The mechanics of shape in prokaryotes
    Siyuan Wang
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Front Biosci (Schol Ed) 5:564-74. 2013
  9. ncbi request reprint Direct measurement of cell wall stress stiffening and turgor pressure in live bacterial cells
    Yi Deng
    Department of Physics, Princeton University, Princeton, New Jersey 08544, USA
    Phys Rev Lett 107:158101. 2011
  10. ncbi request reprint Effect of aberration on height calibration in three-dimensional localization-based microscopy and particle tracking
    Yi Deng
    Department of Physics, Lewis Sigler Institute for Integrative Genomics, Princeton University, 150 Carl Icahn Laboratory, Princeton, New Jersey 08544, USA
    Appl Opt 48:1886-90. 2009

Collaborators

Detail Information

Publications25

  1. pmc Efficient multiple object tracking using mutually repulsive active membranes
    Yi Deng
    Department of Physics, Princeton University, Princeton, New Jersey, United States of America
    PLoS ONE 8:e65769. 2013
    ..Our repulsive active membrane model reaches error rates better than 5 x 10(-6) per fly per second for Drosophila tracking and comparable results for Myxococcus xanthus...
  2. pmc Fast, multiphase volume adaptation to hyperosmotic shock by Escherichia coli
    Teuta Pilizota
    Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, United States of America
    PLoS ONE 7:e35205. 2012
    ..We find that cells are able to recover fully from shocks as high as 1 Osmol/kg using existing systems, but that for larger shocks, protein synthesis is required for full recovery...
  3. pmc Curvature and torsion in growing actin networks
    Joshua W Shaevitz
    Department of Integrative Biology, University of California, Berkeley, Berkeley, CA, USA
    Phys Biol 5:026006. 2008
    ..These results suggest that paths of actin-propelled objects may be attributed to slow changes in curvature, possibly associated with filament debranching, rather than a fixed torque...
  4. pmc Load fluctuations drive actin network growth
    Joshua W Shaevitz
    Department of Integrative Biology, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 104:15688-92. 2007
    ..This conclusion is supported by Monte Carlo simulations of an adhesion-based BR and suggests an important role for membrane tension in the control of actin-based cellular protrusions...
  5. ncbi request reprint Enhanced three-dimensional deconvolution microscopy using a measured depth-varying point-spread function
    Joshua W Shaevitz
    Department of Integrative Biology, University of California, Berkeley 94720, USA
    J Opt Soc Am A Opt Image Sci Vis 24:2622-7. 2007
    ..We present simple convolution and deconvolution algorithms that use the full depth-varying point-spread functions and use these to demonstrate a reduction of elongation artifacts in a reconstructed image of a 2 microm sphere...
  6. pmc Helical insertion of peptidoglycan produces chiral ordering of the bacterial cell wall
    Siyuan Wang
    Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 109:E595-604. 2012
    ..These physical principles of cell growth link the molecular structure of the bacterial cytoskeleton, mechanisms of wall synthesis, and the coordination of cell-wall architecture...
  7. pmc The bacterial actin MreB rotates, and rotation depends on cell-wall assembly
    Sven van Teeffelen
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 108:15822-7. 2011
    ..These findings both broaden the view of cytoskeletal motors and deepen our understanding of the physical basis of bacterial morphogenesis...
  8. ncbi request reprint The mechanics of shape in prokaryotes
    Siyuan Wang
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Front Biosci (Schol Ed) 5:564-74. 2013
    ..We also propose future research directions that will help clarify the mystery of bacterial cell morphogenesis...
  9. ncbi request reprint Direct measurement of cell wall stress stiffening and turgor pressure in live bacterial cells
    Yi Deng
    Department of Physics, Princeton University, Princeton, New Jersey 08544, USA
    Phys Rev Lett 107:158101. 2011
    ..These measurements also indicate that the turgor pressure in living cells E. coli is 29±3  kPa...
  10. ncbi request reprint Effect of aberration on height calibration in three-dimensional localization-based microscopy and particle tracking
    Yi Deng
    Department of Physics, Lewis Sigler Institute for Integrative Genomics, Princeton University, 150 Carl Icahn Laboratory, Princeton, New Jersey 08544, USA
    Appl Opt 48:1886-90. 2009
    ..This effect is nonuniform in depth, and we find that, although molecules below the focal plane are correctly localized, molecules deeper than the focal plane are found to be lower than their actual positions...
  11. ncbi request reprint Image analysis in fluorescence microscopy: bacterial dynamics as a case study
    Sven van Teeffelen
    Princeton University, Department of Molecular Biology, Princeton, NJ, USA
    Bioessays 34:427-36. 2012
    ..Thus, image analysis is not only a toolkit to be applied to new images but also an integral part of the design and implementation of a microscopy experiment...
  12. doi request reprint The molecular origins of chiral growth in walled cells
    Kerwyn Casey Huang
    Department of Bioengineering, Stanford University, Stanford, CA, USA
    Curr Opin Microbiol 15:707-14. 2012
    ..In this review, we highlight experimental and theoretical work that connects molecular-scale organization and structure with the cellular-scale phenomena of rod-shaped growth...
  13. pmc Motor-driven intracellular transport powers bacterial gliding motility
    Mingzhai Sun
    Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08540, USA
    Proc Natl Acad Sci U S A 108:7559-64. 2011
    ..Thus, this study demonstrates the existence of a conserved class of processive intracellular motors in bacteria and shows how these motors have been adapted to produce cell motility...
  14. pmc Actin-like cytoskeleton filaments contribute to cell mechanics in bacteria
    Siyuan Wang
    Department of Molecular Biology, Department of Physics, and Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 107:9182-5. 2010
    ..These data are the first evidence that the bacterial cytoskeleton contributes to the mechanical integrity of a cell in much the same way as it does in eukaryotes...
  15. pmc Plasmolysis and cell shape depend on solute outer-membrane permeability during hyperosmotic shock in E. coli
    Teuta Pilizota
    Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA
    Biophys J 104:2733-42. 2013
    ..Our results give a comprehensive picture of the initial response of E. coli to hyperosmotic shock and offer explanations for seemingly opposing results that have been reported previously. ..
  16. pmc The structure and function of bacterial actin homologs
    Joshua W Shaevitz
    Department of Physics, Princeton University, Princeton, New Jersey 08544, USA
    Cold Spring Harb Perspect Biol 2:a000364. 2010
    ..We also discuss the outstanding puzzles in the field and possible directions where this fast-developing area may progress in the future...
  17. pmc Non-local interaction via diffusible resource prevents coexistence of cooperators and cheaters in a lattice model
    David Bruce Borenstein
    Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, United States of America
    PLoS ONE 8:e63304. 2013
    ..Our results highlight the importance of an accurate treatment of diffusion of shared resources and argue against the generality of the conclusions of game-theoretic lattice models...
  18. ncbi request reprint Spatial Covariance Reconstructive (SCORE) Super-Resolution Fluorescence Microscopy
    Yi Deng
    Department of Physics, Princeton University, Princeton, New Jersey, United States of America
    PLoS ONE 9:e94807. 2014
    ..Our method is insensitive to background and can be applied to different types of fluorescence sources, including but not limited to the organic dyes and quantum dots that we demonstrate in this work. ..
  19. pmc Slow stress propagation in adherent cells
    Michael J Rosenbluth
    Department of Bioengineering, University of California at Berkeley, Berkeley, California 94720, USA
    Biophys J 95:6052-9. 2008
    ....
  20. doi request reprint Super-resolution for a 3D world
    Joshua W Shaevitz
    Nat Methods 5:471-2. 2008
  21. pmc Direct observation of base-pair stepping by RNA polymerase
    Elio A Abbondanzieri
    Department of Applied Physics, Stanford University, Stanford, California 94305, USA
    Nature 438:460-5. 2005
    ..Global fits were inconsistent with a model for movement incorporating a power stroke tightly coupled to pyrophosphate release, but consistent with a brownian ratchet model incorporating a secondary NTP binding site...
  22. pmc Picocalorimetry of transcription by RNA polymerase
    Elio A Abbondanzieri
    Department of Applied Physics, Stanford University, Stanford, California 94305, USA
    Biophys J 89:L61-3. 2005
    ..In contrast, we found little change in either the frequency or the lifetime of off-pathway, paused states, indicating that the energetic barrier to transcriptional pausing is predominantly entropic...
  23. pmc Statistical kinetics of macromolecular dynamics
    Joshua W Shaevitz
    Departments of Physics, Biological Sciences, and Applied Physics, Stanford University, Stanford, CA 94305 5020, USA
    Biophys J 89:2277-85. 2005
    ....
  24. pmc Probing the kinesin reaction cycle with a 2D optical force clamp
    Steven M Block
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 100:2351-6. 2003
    ..Fluctuation analysis shows that the cycle contains at least four transitions, and confirms that hydrolysis remains tightly coupled to stepping. Together, our findings pose challenges for models of kinesin motion...
  25. pmc An automated two-dimensional optical force clamp for single molecule studies
    Matthew J Lang
    Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
    Biophys J 83:491-501. 2002
    ..We present details of force clamp operation and preliminary data showing kinesin motor movement subject to diagonal and forward loads...