Trudi Schupbach

Summary

Affiliation: Princeton University
Country: USA

Publications

  1. pmc The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline
    Attilio Pane
    Department of Molecular Biology, Howard Hughes Medical Institute, Princeton University, NJ 08544, USA
    EMBO J 30:4601-15. 2011
  2. ncbi request reprint Developmental biology: Pipe's smoking guns
    Trudi Schupbach
    HHMI Dept of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Curr Biol 19:R548-50. 2009
  3. pmc zucchini and squash encode two putative nucleases required for rasiRNA production in the Drosophila germline
    Attilio Pane
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Dev Cell 12:851-62. 2007
  4. pmc Cutoff and aubergine mutations result in retrotransposon upregulation and checkpoint activation in Drosophila
    Yu Chen
    Howard Hughes Medical Institute and Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Curr Biol 17:637-42. 2007
  5. ncbi request reprint Deadlock, a novel protein of Drosophila, is required for germline maintenance, fusome morphogenesis and axial patterning in oogenesis and associates with centrosomes in the early embryo
    Kristina Wehr
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Dev Biol 294:406-17. 2006
  6. pmc The Cdc20 (Fzy)/Cdh1-related protein, Cort, cooperates with Fzy in cyclin destruction and anaphase progression in meiosis I and II in Drosophila
    Andrew Swan
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Development 134:891-9. 2007
  7. pmc Squid, Cup, and PABP55B function together to regulate gurken translation in Drosophila
    K Nicole Clouse
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Dev Biol 313:713-24. 2008
  8. pmc CoREST acts as a positive regulator of Notch signaling in the follicle cells of Drosophila melanogaster
    Elena Domanitskaya
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Washington Road, Princeton, NJ 08544 1014, USA
    J Cell Sci 125:399-410. 2012
  9. pmc Drosophila brca2 is required for mitotic and meiotic DNA repair and efficient activation of the meiotic recombination checkpoint
    Martha Klovstad
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
    PLoS Genet 4:e31. 2008
  10. ncbi request reprint Drosophila eggshell is patterned by sequential action of feedforward and feedback loops
    Nir Yakoby
    Department of Chemical Engineering and Lewis Sigler Institute for Integrative Genomics, Princeton University, NJ 08544, USA
    Development 135:343-51. 2008

Research Grants

  1. EGF receptor mediated signaling in Drosphilia
    GERTRUD SCHUPBACH; Fiscal Year: 2009

Collaborators

Detail Information

Publications35

  1. pmc The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline
    Attilio Pane
    Department of Molecular Biology, Howard Hughes Medical Institute, Princeton University, NJ 08544, USA
    EMBO J 30:4601-15. 2011
    ..Our results unveil a link between Cuff activity, heterochromatin assembly and piRNA cluster expression, which is critical for stem-cell and germ-cell development in Drosophila...
  2. ncbi request reprint Developmental biology: Pipe's smoking guns
    Trudi Schupbach
    HHMI Dept of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Curr Biol 19:R548-50. 2009
    ..Now, the first molecular links have been identified between ventral gene expression during oogenesis and the activation of the protease cascade in the early embryo...
  3. pmc zucchini and squash encode two putative nucleases required for rasiRNA production in the Drosophila germline
    Attilio Pane
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Dev Cell 12:851-62. 2007
    ..We show that these defects are due to the inability of zuc and squ mutants to produce repeat-associated small interfering RNAs...
  4. pmc Cutoff and aubergine mutations result in retrotransposon upregulation and checkpoint activation in Drosophila
    Yu Chen
    Howard Hughes Medical Institute and Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Curr Biol 17:637-42. 2007
    ....
  5. ncbi request reprint Deadlock, a novel protein of Drosophila, is required for germline maintenance, fusome morphogenesis and axial patterning in oogenesis and associates with centrosomes in the early embryo
    Kristina Wehr
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Dev Biol 294:406-17. 2006
    ..Our genetic and immunohistological experiments point to a role for Deadlock in microtubule function during oogenesis...
  6. pmc The Cdc20 (Fzy)/Cdh1-related protein, Cort, cooperates with Fzy in cyclin destruction and anaphase progression in meiosis I and II in Drosophila
    Andrew Swan
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Development 134:891-9. 2007
    ....
  7. pmc Squid, Cup, and PABP55B function together to regulate gurken translation in Drosophila
    K Nicole Clouse
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Dev Biol 313:713-24. 2008
    ..These data also provide the first evidence of a link between the complex of commonly used trans-acting factors and Enc, a factor that is required for grk translation...
  8. pmc CoREST acts as a positive regulator of Notch signaling in the follicle cells of Drosophila melanogaster
    Elena Domanitskaya
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Washington Road, Princeton, NJ 08544 1014, USA
    J Cell Sci 125:399-410. 2012
    ..Given its high degree of conservation among species, CoREST probably also functions as a regulator of Notch-dependent cellular events in other organisms...
  9. pmc Drosophila brca2 is required for mitotic and meiotic DNA repair and efficient activation of the meiotic recombination checkpoint
    Martha Klovstad
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
    PLoS Genet 4:e31. 2008
    ..In addition, Brca2 co-immunoprecipitates with the checkpoint protein Rad9, suggesting a direct role for Brca2 in the transduction of the meiotic recombination checkpoint signal...
  10. ncbi request reprint Drosophila eggshell is patterned by sequential action of feedforward and feedback loops
    Nir Yakoby
    Department of Chemical Engineering and Lewis Sigler Institute for Integrative Genomics, Princeton University, NJ 08544, USA
    Development 135:343-51. 2008
    ..This mechanism demonstrates how complex patterns of gene expression can emerge from simple inputs, through the interaction of regulatory network motifs...
  11. ncbi request reprint Hrb27C, Sqd and Otu cooperatively regulate gurken RNA localization and mediate nurse cell chromosome dispersion in Drosophila oogenesis
    Jennifer S Goodrich
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Development 131:1949-58. 2004
    ..All three mutants share another phenotype, persistent polytene nurse cell chromosomes. Our analyses support dual cooperative roles for Sqd, Hrb27C and Otu during Drosophila oogenesis...
  12. ncbi request reprint Drosophila Cks30A interacts with Cdk1 to target Cyclin A for destruction in the female germline
    Andrew Swan
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Development 132:3669-78. 2005
    ..We also find that a second closely related Cks gene, Cks85A, plays a distinct, non-overlapping role in Drosophila, and the two genes cannot functionally replace each other...
  13. ncbi request reprint Quantitative analysis of the GAL4/UAS system in Drosophila oogenesis
    Lea A Goentoro
    Department of Chemical Engineering, Princeton University, Princeton, New Jersey 08544, USA
    Genesis 44:66-74. 2006
    ..The imaging-based strategy described here can be used to quantify other GAL4 drivers in Drosophila and other organisms...
  14. pmc Drosophila PI4KIIIalpha is required in follicle cells for oocyte polarization and Hippo signaling
    Yan Yan
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Development 138:1697-703. 2011
    ..In PI4KIIIalpha mutant follicle cells, Merlin fails to localize to the apical domain. Our analysis of PI4KIIIalpha mutants provides a new link in Hippo signal transduction from the cell membrane to its core kinase cascade...
  15. ncbi request reprint The role of brinker in eggshell patterning
    Yu Chen
    Department of Molecular Biology, Howard Hughes Medical Institute, Princeton University, Princeton, NJ 08544, USA
    Mech Dev 123:395-406. 2006
    ..Operculum fate results when the sum of the level of activation of both pathways reaches a threshold level, and reduction of activity of one pathway can be compensated to some extent by increase in the other pathway...
  16. pmc The Drosophila spn-D gene encodes a RAD51C-like protein that is required exclusively during meiosis
    Uri Abdu
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Genetics 165:197-204. 2003
    ..As there is no apparent ortholog of the meiosis-specific DMC1 gene in the Drosophila genome, and given their meiosis-specific requirement, we suggest that spn-B and spn-D may have a function comparable to DMC1...
  17. ncbi request reprint Drosophila female meiosis and embryonic syncytial mitosis use specialized Cks and CDC20 proteins for cyclin destruction
    Andrew Swan
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Cell Cycle 4:1332-4. 2005
    ..These results indicate that anaphase progression in female meiosis and the early embryo are under unique control in Drosophila...
  18. pmc The dynamics of fluorescently labeled endogenous gurken mRNA in Drosophila
    Angela M Jaramillo
    Howard Hughes Medical Institute, Princeton University, NJ 08544, USA
    J Cell Sci 121:887-94. 2008
    ..Taken together, we have observed the nature of localized grk mRNA in live oocytes and propose that its maintenance changes from a dynamic to a static process as oogenesis progresses...
  19. ncbi request reprint Cct1, a phosphatidylcholine biosynthesis enzyme, is required for Drosophila oogenesis and ovarian morphogenesis
    Tripti Gupta
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Development 130:6075-87. 2003
    ..These data provide the first evidence for a specific role for CCT, and thus for phosphatidylcholine, in patterning during development...
  20. pmc Regulation of somatic myosin activity by protein phosphatase 1β controls Drosophila oocyte polarization
    Yi Sun
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Development 138:1991-2001. 2011
    ..Interestingly, our results also demonstrate a role of PP1β in generating the polarizing signal independently of Notch, indicating a direct involvement of somatic myosin activity in axis formation...
  21. pmc The vacuolar proton pump, V-ATPase, is required for notch signaling and endosomal trafficking in Drosophila
    Yan Yan
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Dev Cell 17:387-402. 2009
    ..Our results demonstrate that Rbcn-3 affects Notch signaling and trafficking through regulating V-ATPase function, which implies that the acidification of an intracellular compartment in the receiving cells is crucial for signaling...
  22. pmc A combinatorial code for pattern formation in Drosophila oogenesis
    Nir Yakoby
    Lewis Sigler Institute for Integrative Genomics and Department of Chemical Engineering, Princeton University, Princeton, NJ 08544, USA
    Dev Cell 15:725-37. 2008
    ..The proposed combinatorial framework allows systematic analysis of the diversity and dynamics of two-dimensional transcriptional patterns and guides future studies of gene regulation...
  23. pmc Crag regulates epithelial architecture and polarized deposition of basement membrane proteins in Drosophila
    Natalie Denef
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Dev Cell 14:354-64. 2008
    ..This defect is not generally observed in mutants affecting epithelial integrity. We propose that Crag plays a unique role in organizing epithelial architecture by regulating the polarized secretion of BM proteins...
  24. ncbi request reprint Encore facilitates SCF-Ubiquitin-proteasome-dependent proteolysis during Drosophila oogenesis
    Johanna Talavera Ohlmeyer
    HHMI Molecular Biology Department, Princeton University, Princeton, NJ 08544, USA
    Development 130:6339-49. 2003
    ....
  25. ncbi request reprint Quantitative models of developmental pattern formation
    Gregory T Reeves
    Department of Chemical Engineering and Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA
    Dev Cell 11:289-300. 2006
    ..Using a number of problems in fruit fly development, we discuss how models can be used to test the feasibility of proposed patterning mechanisms and characterize their systems-level properties...
  26. pmc Three-dimensional epithelial morphogenesis in the developing Drosophila egg
    Miriam Osterfield
    Lewis Sigler Institute for Integrative Genomics, Princeton, NJ 08544, USA
    Dev Cell 24:400-10. 2013
    ..Computational modeling shows that this mechanism could explain the main features of tissue deformation and cell rearrangements observed during three-dimensional morphogenesis...
  27. doi request reprint Pattern formation by receptor tyrosine kinases: analysis of the Gurken gradient in Drosophila oogenesis
    Lily S Cheung
    Department of Chemical and Biological Engineering and Lewis Sigler Institute for Integrative Genomics, Princeton University, NJ J08544, USA
    Curr Opin Genet Dev 21:719-25. 2011
    ..We review recent quantitative studies of this mechanism in Drosophila oogenesis, an established genetic model of signaling through the Epidermal Growth Factor Receptor (EGFR), a highly conserved RTK...
  28. pmc Quantifying the Gurken morphogen gradient in Drosophila oogenesis
    Lea A Goentoro
    Department of Chemical Engineering and Lewis Sigler Institute for Integrative Genomics, Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Dev Cell 11:263-72. 2006
    ..This provides a direct characterization of the shape of the Gurken gradient and demonstrates how parameter estimation techniques can be used to quantify morphogen gradients in development...
  29. ncbi request reprint Activation of a meiotic checkpoint during Drosophila oogenesis regulates the translation of Gurken through Chk2/Mnk
    Uri Abdu
    HHMI, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Curr Biol 12:1645-51. 2002
    ..However, the components of this checkpoint in flies have not been completely elucidated...
  30. pmc Rabconnectin-3 is a functional regulator of mammalian Notch signaling
    Nilay Sethi
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    J Biol Chem 285:34757-64. 2010
    ..Collectively, these findings define an important role for Rabconnectin-3 and V-ATPase activity in the Notch signaling pathway in mammalian cells...
  31. ncbi request reprint Patterning: JAK-STAT signalling in the Drosophila follicular epithelium
    Natalie Denef
    HHMI Department of Molecular Biology, Princeton University, New Jersey 08544, USA
    Curr Biol 13:R388-90. 2003
    ..During Drosophila oogenesis the follicular epithelium becomes subdivided into distinct cell populations. New reports have established that the Janus kinase (JAK) signalling pathway plays an important role in this process...
  32. pmc Phantom, a cytochrome P450 enzyme essential for ecdysone biosynthesis, plays a critical role in the control of border cell migration in Drosophila
    Elena Domanitskaya
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States
    Dev Biol 386:408-18. 2014
    ..This "egg chamber autonomous" ecdysone synthesis constitutes a useful way to regulate the individual maturation of the asynchronous egg chambers present in the Drosophila ovary. ..
  33. ncbi request reprint Half pint regulates alternative splice site selection in Drosophila
    Cheryl Van Buskirk
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Dev Cell 2:343-53. 2002
    ..In particular, hfp mutants display striking defects in the developmentally regulated splicing of ovarian tumor (otu). Furthermore, transgenic expression of the missing otu splice form can rescue the ovarian phenotypes of hfp...
  34. pmc Polarized deposition of basement membrane proteins depends on Phosphatidylinositol synthase and the levels of Phosphatidylinositol 4,5-bisphosphate
    Olivier Devergne
    Howard Hughes Medical Institute and Department of Molecular Biology, Princeton University, Princeton, NJ 08544
    Proc Natl Acad Sci U S A 111:7689-94. 2014
    ..We propose that PIP2, through the control of Crag's subcellular localization, restricts the secretion of BM proteins to the basal side. ..
  35. pmc A novel inhibitor of amyloid β (Aβ) peptide aggregation: from high throughput screening to efficacy in an animal model of Alzheimer disease
    Angela Fortner McKoy
    Department of Chemistry, Princeton University, Princeton, New Jersey 08544, USA
    J Biol Chem 287:38992-9000. 2012
    ..Most importantly, treatment with D737 increases the life span and locomotive ability of flies in a Drosophila melanogaster model of Alzheimer disease...

Research Grants2

  1. EGF receptor mediated signaling in Drosphilia
    GERTRUD SCHUPBACH; Fiscal Year: 2009
    ..It will also define downstream effector pathways operating in the follicle cell epithelium, which serves as a model system for epithelial development and differentiation. ..