Natividad Ruiz

Summary

Affiliation: Princeton University
Country: USA

Publications

  1. ncbi Advances in understanding bacterial outer-membrane biogenesis
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Nat Rev Microbiol 4:57-66. 2006
  2. pmc Bioinformatics identification of MurJ (MviN) as the peptidoglycan lipid II flippase in Escherichia coli
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 105:15553-7. 2008
  3. pmc Identification of two inner-membrane proteins required for the transport of lipopolysaccharide to the outer membrane of Escherichia coli
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 105:5537-42. 2008
  4. pmc Lipoprotein LptE is required for the assembly of LptD by the beta-barrel assembly machine in the outer membrane of Escherichia coli
    Gitanjali Chimalakonda
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 108:2492-7. 2011
  5. ncbi Probing the barrier function of the outer membrane with chemical conditionality
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    ACS Chem Biol 1:385-95. 2006
  6. pmc Nonconsecutive disulfide bond formation in an essential integral outer membrane protein
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 107:12245-50. 2010
  7. ncbi Chemical conditionality: a genetic strategy to probe organelle assembly
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Cell 121:307-17. 2005
  8. ncbi Sensing external stress: watchdogs of the Escherichia coli cell envelope
    Natividad Ruiz
    Molecular Biology Department, Princeton University, Princeton, NJ 08544, USA
    Curr Opin Microbiol 8:122-6. 2005
  9. pmc RpoS proteolysis is regulated by a mechanism that does not require the SprE (RssB) response regulator phosphorylation site
    Celeste N Peterson
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    J Bacteriol 186:7403-10. 2004
  10. pmc A suppressor of cell death caused by the loss of sigmaE downregulates extracytoplasmic stress responses and outer membrane vesicle production in Escherichia coli
    Julie E Button
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    J Bacteriol 189:1523-30. 2007

Collaborators

  • THOMAS SILHAVY
  • Gitanjali Chimalakonda
  • Daniel Kahne
  • Julie E Button
  • Tao Wu
  • Celeste N Peterson
  • Shu Sin Chng
  • Ronald A Garner
  • Seokhee Kim
  • Juliana Malinverni

Detail Information

Publications13

  1. ncbi Advances in understanding bacterial outer-membrane biogenesis
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Nat Rev Microbiol 4:57-66. 2006
    ..Here, we review the new approaches that have led to the recent discovery of components of the machinery involved in the biogenesis of this distinctive cellular organelle...
  2. pmc Bioinformatics identification of MurJ (MviN) as the peptidoglycan lipid II flippase in Escherichia coli
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 105:15553-7. 2008
    ..Because of its essential nature, MurJ could serve as a target in the continuing search for antimicrobial compounds...
  3. pmc Identification of two inner-membrane proteins required for the transport of lipopolysaccharide to the outer membrane of Escherichia coli
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 105:5537-42. 2008
    ..We propose that these two proteins, which we have renamed LptF and LptG, respectively, are the missing transmembrane components of the ABC transporter that, together with LptB, functions to extract LPS from the IM en route to the OM...
  4. pmc Lipoprotein LptE is required for the assembly of LptD by the beta-barrel assembly machine in the outer membrane of Escherichia coli
    Gitanjali Chimalakonda
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 108:2492-7. 2011
    ..We show that lptE6 and both suppressor mutations affect the assembly of the LptD/E complex and suggest that the lipoprotein LptE interacts with LptD while this protein is being assembled by the β-barrel assembly machine...
  5. ncbi Probing the barrier function of the outer membrane with chemical conditionality
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    ACS Chem Biol 1:385-95. 2006
    ..The remarkable chemical specificity of the imp4213 suppressors provides insights into the molecular nature of the OM permeability barrier...
  6. pmc Nonconsecutive disulfide bond formation in an essential integral outer membrane protein
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 107:12245-50. 2010
    ..Thus, oxidation is completed only after LptD interacts with LptE, an interaction that occurs at the outer membrane and seems necessary for LptD folding...
  7. ncbi Chemical conditionality: a genetic strategy to probe organelle assembly
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Cell 121:307-17. 2005
    ..We posit that panels of toxic small molecules will be useful for generating chemical conditionalities that enable identification of genes required for organelle assembly in other organisms...
  8. ncbi Sensing external stress: watchdogs of the Escherichia coli cell envelope
    Natividad Ruiz
    Molecular Biology Department, Princeton University, Princeton, NJ 08544, USA
    Curr Opin Microbiol 8:122-6. 2005
    ..In this review, we discuss the recent progress in our understanding of the Cpx and sigmaE systems and compare how both function to maintain the integrity of the cell envelope...
  9. pmc RpoS proteolysis is regulated by a mechanism that does not require the SprE (RssB) response regulator phosphorylation site
    Celeste N Peterson
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    J Bacteriol 186:7403-10. 2004
    ..Furthermore, our results indicate that phosphorylation of wild-type SprE occurs by a mechanism that is independent of acetyl phosphate...
  10. pmc A suppressor of cell death caused by the loss of sigmaE downregulates extracytoplasmic stress responses and outer membrane vesicle production in Escherichia coli
    Julie E Button
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    J Bacteriol 189:1523-30. 2007
    ..We present a model in which sigma(E) is not essential per se but, rather, we propose that rpoE mutant cells die, possibly because they overreact to the absence of this sigma factor by triggering a cell death signal...
  11. pmc Transport of lipopolysaccharide across the cell envelope: the long road of discovery
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Nat Rev Microbiol 7:677-83. 2009
    ....
  12. ncbi Identification of a multicomponent complex required for outer membrane biogenesis in Escherichia coli
    Tao Wu
    Department of Chemistry, Princeton University, Princeton, New Jersey 08544, USA
    Cell 121:235-45. 2005
    ..These genetic interactions suggest a role for YfgL, one of the lipoprotein components of the protein assembly complex, in a homeostatic control mechanism that coordinates the overall OM assembly process...
  13. pmc Constitutive activation of the Escherichia coli Pho regulon upregulates rpoS translation in an Hfq-dependent fashion
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    J Bacteriol 185:5984-92. 2003
    ..This upregulation of rpoS translation is Hfq dependent, suggesting the involvement of a small regulatory RNA (sRNA). The transcription of this yet-to-be-identified sRNA is regulated by the PhoBR two-component system...