A James Link

Summary

Affiliation: Princeton University
Country: USA

Publications

  1. ncbi request reprint Expanding the chemical diversity of lasso peptide MccJ25 with genetically encoded noncanonical amino acids
    Frank J Piscotta
    Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA
    Chem Commun (Camb) 51:409-12. 2015
  2. ncbi request reprint Protein stapling via azide-alkyne ligation
    Diya M Abdeljabbar
    Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA
    Chem Commun (Camb) 50:14900-3. 2014
  3. ncbi request reprint Lasso peptides: structure, function, biosynthesis, and engineering
    Mikhail O Maksimov
    Department of Chemical and Biological Engineering, Princeton University, NJ 08544, USA
    Nat Prod Rep 29:996-1006. 2012
  4. ncbi request reprint Synthesis of the functionalizable methionine surrogate azidohomoalanine using Boc-homoserine as precursor
    A James Link
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91106, USA
    Nat Protoc 2:1884-7. 2007
  5. ncbi request reprint Preparation of the functionalizable methionine surrogate azidohomoalanine via copper-catalyzed diazo transfer
    A James Link
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91106, USA
    Nat Protoc 2:1879-83. 2007
  6. pmc Computational design of the lasso peptide antibiotic microcin J25
    Si Jia Pan
    Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA
    Protein Eng Des Sel 24:275-82. 2011
  7. ncbi request reprint Labeling, detection and identification of newly synthesized proteomes with bioorthogonal non-canonical amino-acid tagging
    Daniela C Dieterich
    Division of Biology, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California 91125, USA
    Nat Protoc 2:532-40. 2007
  8. pmc Discovery of aminoacyl-tRNA synthetase activity through cell-surface display of noncanonical amino acids
    A James Link
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 103:10180-5. 2006
  9. pmc Precursor-centric genome-mining approach for lasso peptide discovery
    Mikhail O Maksimov
    Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 109:15223-8. 2012
  10. ncbi request reprint A single genomic copy of an engineered methionyl-tRNA synthetase enables robust incorporation of azidonorleucine into recombinant proteins in E. coli
    Diya M Abdeljabbar
    Department of Chemical Engineering, Princeton University, A207 Engineering Quadrangle, Princeton, New Jersey 08544, USA
    J Am Chem Soc 131:17078-9. 2009

Research Grants

  1. Optimization of Membrane Protein Biogenesis
    AARON LINK; Fiscal Year: 2007

Collaborators

  • Erin M Schuman
  • Daniela C Dieterich
  • Wenshe R Liu
  • Istvan Pelczer
  • Si Jia Pan
  • Siyan Zhang
  • Mikhail O Maksimov
  • Diya M Abdeljabbar
  • Wai Ling Cheung
  • Frank J Piscotta
  • A James Link
  • Alan S Futran
  • Jakub Rajniak
  • Robert K Prud'homme
  • Manu S Mannoor
  • Christodoulos A Floudas
  • Lucy Y Xu
  • Jingjing Sun
  • Tae Hyeon Yoo
  • Jeffery M Tharp
  • Rony Seger
  • Stanislav Y Shvartsman
  • Angel Long
  • Hank J Song
  • Kiat Hwa Chan
  • Ho Ki Fung
  • Michael C McAlpine
  • Nicole Clarke
  • Thomas J Klein
  • Meghan L Bellows
  • David A Tirrell

Detail Information

Publications31

  1. ncbi request reprint Expanding the chemical diversity of lasso peptide MccJ25 with genetically encoded noncanonical amino acids
    Frank J Piscotta
    Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA
    Chem Commun (Camb) 51:409-12. 2015
    ..Given the rapid expansion of the genetically encoded ncAA pool, this study is the first to demonstrate an expedient method to significantly increase the chemical diversity of lasso peptides. ..
  2. ncbi request reprint Protein stapling via azide-alkyne ligation
    Diya M Abdeljabbar
    Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA
    Chem Commun (Camb) 50:14900-3. 2014
    ..Additionally, stapling the core of the small, globular protein G resulted in improved binding to its target, immunoglobulin G. ..
  3. ncbi request reprint Lasso peptides: structure, function, biosynthesis, and engineering
    Mikhail O Maksimov
    Department of Chemical and Biological Engineering, Princeton University, NJ 08544, USA
    Nat Prod Rep 29:996-1006. 2012
    ..Several studies on the engineering of new or improved function into lasso peptides are highlighted, and unanswered questions in the field are also described...
  4. ncbi request reprint Synthesis of the functionalizable methionine surrogate azidohomoalanine using Boc-homoserine as precursor
    A James Link
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91106, USA
    Nat Protoc 2:1884-7. 2007
    ..An alternative route to AHA is presented in a companion paper. This synthesis can be completed in 5 days...
  5. ncbi request reprint Preparation of the functionalizable methionine surrogate azidohomoalanine via copper-catalyzed diazo transfer
    A James Link
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91106, USA
    Nat Protoc 2:1879-83. 2007
    ..An alternate protocol for the preparation of AHA is presented in a companion paper. The synthesis and purification of AHA via the route described herein can be completed in 3-4 days...
  6. pmc Computational design of the lasso peptide antibiotic microcin J25
    Si Jia Pan
    Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA
    Protein Eng Des Sel 24:275-82. 2011
    ..Furthermore, this work provides evidence that a large amount of amino acid variation is tolerated by the lasso peptide fold...
  7. ncbi request reprint Labeling, detection and identification of newly synthesized proteomes with bioorthogonal non-canonical amino-acid tagging
    Daniela C Dieterich
    Division of Biology, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California 91125, USA
    Nat Protoc 2:532-40. 2007
    ..This protocol can be completed in 5 days...
  8. pmc Discovery of aminoacyl-tRNA synthetase activity through cell-surface display of noncanonical amino acids
    A James Link
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 103:10180-5. 2006
    ..The Leu-13 --> Gly (L13G) mutation is found in each of the three MetRS mutants, and the MetRS variant containing this single mutation is highly efficient in producing recombinant proteins that contain azidonorleucine...
  9. pmc Precursor-centric genome-mining approach for lasso peptide discovery
    Mikhail O Maksimov
    Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 109:15223-8. 2012
    ..The solution structure of astexin-1 was determined revealing a unique topology that is stabilized by hydrogen bonding between segments of the peptide...
  10. ncbi request reprint A single genomic copy of an engineered methionyl-tRNA synthetase enables robust incorporation of azidonorleucine into recombinant proteins in E. coli
    Diya M Abdeljabbar
    Department of Chemical Engineering, Princeton University, A207 Engineering Quadrangle, Princeton, New Jersey 08544, USA
    J Am Chem Soc 131:17078-9. 2009
    ..Since this mutant MetRS has been introduced into the genome, as opposed to a plasmid, M15MA metG* is genetically stable...
  11. ncbi request reprint Trichoderma reesei cellobiohydrolase II is associated with the outer membrane when overexpressed in Escherichia coli
    Diya M Abdeljabbar
    Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA
    Biotechnol Lett 34:91-6. 2012
    ..These results highlight the difficulties in producing fungal cellulases in bacterial hosts and provide a stepping stone for future cellulase engineering efforts...
  12. ncbi request reprint Bcl-2 family interactome analysis using bacterial surface display
    Siyan Zhang
    Department of Chemical and Biological Engineering, A207 Engineering Quadrangle, Princeton University, Princeton, NJ 08544, USA
    Integr Biol (Camb) 3:823-31. 2011
    ..The interactome data from this study has implications both in basic research on apoptosis and in the design of peptidic cancer therapeutics...
  13. pmc Selective identification of newly synthesized proteins in mammalian cells using bioorthogonal noncanonical amino acid tagging (BONCAT)
    Daniela C Dieterich
    Division of Biology, Howard Hughes Medical Institute, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 103:9482-7. 2006
    ..This technology makes it possible to address the temporal and spatial characteristics of newly synthesized proteomes in any cell type...
  14. ncbi request reprint Engineered gene clusters for the production of the antimicrobial peptide microcin J25
    Si Jia Pan
    Department of Chemical Engineering, Princeton University, Princeton, NJ 08544, USA
    Protein Expr Purif 71:200-6. 2010
    ..Furthermore, we provide evidence that control of the natural MccJ25 gene cluster is at the transcriptional level. The observations herein provide design parameters for large-scale production of MccJ25 for biotechnological applications...
  15. ncbi request reprint Construction of a single polypeptide that matures and exports the lasso peptide microcin J25
    Si Jia Pan
    Department of Chemical and Biological Engineering, Princeton University, A207 Engineering Quadrangle, Princeton, NJ 08544, USA
    Chembiochem 13:367-70. 2012
    ..We demonstrate that fusions of the enzymes with intervening linkers can produce MccJ25. Even a 151 kDa tripartite fusion between the ABC transporter and the two enzymes is capable of producing and exporting MccJ25...
  16. pmc Evolution of a fluorinated green fluorescent protein
    Tae Hyeon Yoo
    Division of Chemistry and Chemical Engineering, Joseph J Jacobs Institute for Molecular Engineering for Medicine, California Institute of Technology, 1200 East California Boulevard, MC210 41, Pasadena, CA 91125 4100, USA
    Proc Natl Acad Sci U S A 104:13887-90. 2007
    ..The success of this approach demonstrates the feasibility of engineering functional proteins containing many copies of abiological amino acid constituents...
  17. ncbi request reprint Cell surface labeling of Escherichia coli via copper(I)-catalyzed [3+2] cycloaddition
    A James Link
    Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, California 91125, USA
    J Am Chem Soc 125:11164-5. 2003
    ..Incorporation of an unnatural amino acid can thus be determined on a single-cell basis...
  18. ncbi request reprint Presentation and detection of azide functionality in bacterial cell surface proteins
    A James Link
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    J Am Chem Soc 126:10598-602. 2004
    ..Azidoalanine was previously believed to be silent with regard to the cellular protein synthesis machinery...
  19. ncbi request reprint The role of a conserved threonine residue in the leader peptide of lasso peptide precursors
    Si Jia Pan
    Department of Chemical and Biological Engineering, A207 Engineering Quadrangle, Princeton University, Princeton, NJ 08544, USA
    Chem Commun (Camb) 48:1880-2. 2012
    ..These findings suggest a model for lasso peptide biosynthesis in which the Thr sidechain is a recognition element for the lasso peptide maturation machinery...
  20. pmc Reconstitution and engineering of apoptotic protein interactions on the bacterial cell surface
    Jingjing Sun
    Department of Chemical Engineering, Princeton University, Princeton, NJ 08544, USA
    J Mol Biol 394:297-305. 2009
    ..The best variants obtained from this library exhibit an apparent K(d) to Bcl-x(L) 4-fold lower than that of wild-type Bak(72-87)...
  21. ncbi request reprint Sequence diversity in the lasso peptide framework: discovery of functional microcin J25 variants with multiple amino acid substitutions
    Si Jia Pan
    Department of Chemical and Biological Engineering, Princeton University, Princeton, New Jersey 08544, USA
    J Am Chem Soc 133:5016-23. 2011
    ..The results described here provide further evidence that diverse amino acid sequences can be tolerated by the rigid lasso peptide fold...
  22. ncbi request reprint Discovery and characterization of an isopeptidase that linearizes lasso peptides
    Mikhail O Maksimov
    Department of Chemical and Biological Engineering, Princeton University, Princeton, New Jersey 08544, USA
    J Am Chem Soc 135:12038-47. 2013
    ..We also use this isopeptidase as a tool to study evolutionary relationships between lasso peptide gene clusters. ..
  23. ncbi request reprint A comparison of two strategies for affinity maturation of a BH3 peptide toward pro-survival Bcl-2 proteins
    Siyan Zhang
    Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA
    ACS Synth Biol 1:89-98. 2012
    ..HeLa cell killing studies demonstrate that the affinity matured Bak BH3 variants retain their expected biological function...
  24. ncbi request reprint Prospecting genomes for lasso peptides
    Mikhail O Maksimov
    207 Hoyt Laboratory, Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ, 08544, USA
    J Ind Microbiol Biotechnol 41:333-44. 2014
    ..This review presents the bioinformatic methods used to discover novel lasso peptides and describes how such analyses have afforded insights into the biosynthesis and evolution of this peptide class. ..
  25. ncbi request reprint Stress responses to heterologous membrane protein expression in Escherichia coli
    Lucy Y Xu
    Department of Chemical Engineering, Princeton University, A207 Engineering Quadrangle, Princeton, NJ 08544, USA
    Biotechnol Lett 31:1775-82. 2009
    ..Co-expression of CB1 and FtsH led to an additive response in these four reporter genes suggesting that the stresses may be occurring via different physiological mechanisms...
  26. ncbi request reprint Synthesis and evaluation of clickable block copolymers for targeted nanoparticle drug delivery
    Siyan Zhang
    Departments of Chemical and Biological Engineering and Molecular Biology, Princeton University, Princeton, New Jersey 08540, United States
    Mol Pharm 9:2228-36. 2012
    ..Using the copper-catalyzed azide-alkyne ligation reaction, both ligands exhibited good conjugation efficiency even when low concentrations of ligands were used. ..
  27. pmc ERK as a model for systems biology of enzyme kinetics in cells
    Alan S Futran
    Department of Chemical and Biological Engineering, Princeton University, Princeton, USA
    Curr Biol 23:R972-9. 2013
    ....
  28. pmc Electrical detection of pathogenic bacteria via immobilized antimicrobial peptides
    Manu S Mannoor
    Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 107:19207-12. 2010
    ....
  29. ncbi request reprint Reassignment of sense codons in vivo
    A James Link
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA
    Methods 36:291-8. 2005
    ..Codon reassignment is leading to important advances in protein engineering and bioorganic chemistry. Here we summarize some of those advances, and provide detailed protocols for codon reassignment...
  30. ncbi request reprint Much of the microcin J25 leader peptide is dispensable
    Wai Ling Cheung
    Department of Chemical Engineering, Princeton University, A207 Engineering Quadrangle, Princeton, New Jersey 08544, USA
    J Am Chem Soc 132:2514-5. 2010
    ..In the absence of detailed structural and biochemical data about McjB and McjC, these studies allow us to propose a putative role for the leader peptide as a simple motif for docking of the McjA preprotein in the maturation enzymes...
  31. ncbi request reprint Block copolymer nanoparticles as nanobeads for the polymerase chain reaction
    Siyan Zhang
    Department of Chemical and Biological Engineering, Princeton University, Princeton, New Jersey 08544, United States
    Nano Lett 11:1723-6. 2011
    ..To this end, we have assembled polymeric nanobeads that efficiently template PCR, resulting in DNA-decorated "nanobeads" with a high extent of functionalization...

Research Grants1

  1. Optimization of Membrane Protein Biogenesis
    AARON LINK; Fiscal Year: 2007
    ..coli will be developed. Such a platform will be of great utility to researchers interested in the structural and biochemical properties of membrane proteins. [unreadable] [unreadable] [unreadable]..