FREDERICK HUGHSON

Summary

Affiliation: Princeton University
Country: USA

Publications

  1. pmc Crystal Structures of the Sec1/Munc18 (SM) Protein Vps33, Alone and Bound to the Homotypic Fusion and Vacuolar Protein Sorting (HOPS) Subunit Vps16*
    Richard W Baker
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
    PLoS ONE 8:e67409. 2013
  2. pmc Molecular organization of the COG vesicle tethering complex
    Joshua A Lees
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA
    Nat Struct Mol Biol 17:1292-7. 2010
  3. pmc Structure and mechanism in membrane trafficking
    Frederick M Hughson
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Curr Opin Cell Biol 22:454-60. 2010
  4. ncbi request reprint Membrane fusion: structure snared at last
    F M Hughson
    Department of Molecular Biology, Princeton University, New Jersey, 08544, USA
    Curr Biol 9:R49-52. 1999
  5. ncbi request reprint SNARE protein structure and function
    Daniel Ungar
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Annu Rev Cell Dev Biol 19:493-517. 2003
  6. ncbi request reprint Structural analysis of conserved oligomeric Golgi complex subunit 2
    Lorraine F Cavanaugh
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    J Biol Chem 282:23418-26. 2007
  7. ncbi request reprint Subunit architecture of the conserved oligomeric Golgi complex
    Daniel Ungar
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    J Biol Chem 280:32729-35. 2005
  8. pmc Ligand-induced asymmetry in histidine sensor kinase complex regulates quorum sensing
    Matthew B Neiditch
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Cell 126:1095-108. 2006
  9. pmc Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex
    Arati Tripathi
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Nat Struct Mol Biol 16:114-23. 2009
  10. doi request reprint Both layers of the COPII coat come into view
    Frederick M Hughson
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Cell 134:384-5. 2008

Research Grants

  1. Structural Analysis of Golgi Trafficking Proteins
    FREDERICK HUGHSON; Fiscal Year: 2007
  2. Structure-Function Analysis of AI-2 Quorum Sensing
    FREDERICK HUGHSON; Fiscal Year: 2007
  3. Structural Analysis of Golgi Trafficking Proteins
    FREDERICK HUGHSON; Fiscal Year: 2009
  4. Structure-Function Analysis of AI-2 Quorum Sensing
    Frederick M Hughson; Fiscal Year: 2010
  5. Structural Analysis of Golgi Trafficking Proteins
    Frederick M Hughson; Fiscal Year: 2010
  6. STRUCTURAL ANALYSIS OF INTRACELLULAR TRAFFICKING PROTEIN
    FREDERICK HUGHSON; Fiscal Year: 2002
  7. STRUCTURE/FUNCTION OF GTC, A NOVEL GOLGI PROTEIN COMPLEX
    FREDERICK HUGHSON; Fiscal Year: 2002
  8. Structure-Function Analysis of AI-2 Quorum Sensing
    Frederick M Hughson; Fiscal Year: 2010

Collaborators

  • Daniel Ungar
  • M Krieger
  • Toshihiko Oka
  • Thomas Walz
  • Josep Rizo
  • Philip D Jeffrey
  • Matthew B Neiditch
  • Arati Tripathi
  • Yi Ren
  • Richard W Baker
  • I Mei Yu
  • Joshua A Lees
  • Calvin K Yip
  • Doug Barrick
  • Lorraine F Cavanaugh
  • Bonnie L Bassler
  • Stephen T Miller
  • Mark E Zweifel
  • Mary Munson
  • David Huie
  • Brian C Richardson
  • Xiaocheng Chen
  • Istvan Pelczer
  • Michael J Federle
  • Danielle L Swem
  • Robert C Kelly
  • Audra J Pompeani
  • Karina B Xavier
  • Michiko E Taga
  • Shawn R Campagna
  • Martin F Semmelhack
  • Daniel J Leahy

Detail Information

Publications20

  1. pmc Crystal Structures of the Sec1/Munc18 (SM) Protein Vps33, Alone and Bound to the Homotypic Fusion and Vacuolar Protein Sorting (HOPS) Subunit Vps16*
    Richard W Baker
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
    PLoS ONE 8:e67409. 2013
    ..6 Å resolution, reveals the structural basis for this interaction. Despite the extensive interface between the two HOPS subunits, the conformation of Vps33 is only subtly affected by binding to Vps16...
  2. pmc Molecular organization of the COG vesicle tethering complex
    Joshua A Lees
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA
    Nat Struct Mol Biol 17:1292-7. 2010
    ..Our results suggest that the central region of the Cog1-Cog2-Cog3-Cog4 complex, as well as the distal regions of at least two legs, all participate in interactions with other components of the intracellular trafficking machinery...
  3. pmc Structure and mechanism in membrane trafficking
    Frederick M Hughson
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Curr Opin Cell Biol 22:454-60. 2010
    ..Here, we discuss integrated structural studies of proteins whose assembly shapes membranes into vesicles and tubules, before turning to the so-called tethering factors that appear to orchestrate vesicle docking and fusion...
  4. ncbi request reprint Membrane fusion: structure snared at last
    F M Hughson
    Department of Molecular Biology, Princeton University, New Jersey, 08544, USA
    Curr Biol 9:R49-52. 1999
    ..Its topological similarity to viral fusion proteins suggests how the SNARE complex might facilitate membrane fusion...
  5. ncbi request reprint SNARE protein structure and function
    Daniel Ungar
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Annu Rev Cell Dev Biol 19:493-517. 2003
    ....
  6. ncbi request reprint Structural analysis of conserved oligomeric Golgi complex subunit 2
    Lorraine F Cavanaugh
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    J Biol Chem 282:23418-26. 2007
    ..These structures may represent platforms for interaction with other trafficking proteins including SNAREs (soluble N-ethylmaleimide factor attachment protein receptors) and Rabs...
  7. ncbi request reprint Subunit architecture of the conserved oligomeric Golgi complex
    Daniel Ungar
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    J Biol Chem 280:32729-35. 2005
    ..Vasile, E., Penman, M., Novina, C. D., Dykxhoorn, D. M., Ungar, D., Hughson, F. M., and Krieger, M. (2005) J. Biol. Chem. 280, 32736-32745)...
  8. pmc Ligand-induced asymmetry in histidine sensor kinase complex regulates quorum sensing
    Matthew B Neiditch
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Cell 126:1095-108. 2006
    ..We propose that formation of this asymmetric quaternary structure is responsible for repressing the kinase activity of both LuxQ subunits and triggering the transition of V. harveyi into quorum-sensing mode...
  9. pmc Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex
    Arati Tripathi
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Nat Struct Mol Biol 16:114-23. 2009
    ..Our results map out two alternative protein-interaction networks capable of tethering COPI-coated vesicles, via the Dsl1p complex, to ER membranes...
  10. doi request reprint Both layers of the COPII coat come into view
    Frederick M Hughson
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Cell 134:384-5. 2008
    ..Stagg et al. (2008) have now visualized the double-layered COPII coat using electron cryomicroscopy, providing insight into how coats are assembled to accommodate cargo of different sizes...
  11. ncbi request reprint Salmonella typhimurium recognizes a chemically distinct form of the bacterial quorum-sensing signal AI-2
    Stephen T Miller
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Mol Cell 15:677-87. 2004
    ....
  12. ncbi request reprint Conformational regulation of SNARE assembly and disassembly in vivo
    Mary Munson
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    J Biol Chem 277:9375-81. 2002
    ..Our findings indicate that elevated levels of SNARE complexes can be toxic and that these levels are normally controlled by the SNARE disassembly machinery, by the limited availability of Sec9p, and by the closed conformation of Sso1p...
  13. pmc A structure-based mechanism for vesicle capture by the multisubunit tethering complex Dsl1
    Yi Ren
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Cell 139:1119-29. 2009
    ..Our results suggest that even the simplest MTC may be capable of orchestrating vesicle capture, uncoating, and fusion...
  14. doi request reprint Tethering factors as organizers of intracellular vesicular traffic
    I Mei Yu
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Annu Rev Cell Dev Biol 26:137-56. 2010
    ..The combination of these and other approaches has led to exciting progress toward understanding how these essential nanomachines work...
  15. ncbi request reprint The regulation of histidine sensor kinase complexes by quorum sensing signal molecules
    Matthew B Neiditch
    Department of Molecular Biology, Princeton University, Princeton, NJ, USA
    Methods Enzymol 423:250-63. 2007
    ..Methods are presented for the overproduction, purification, crystallization, and functional characterization of LuxPQ's ligand-binding (periplasmic) domain...
  16. ncbi request reprint Retrograde transport on the COG railway
    Daniel Ungar
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Trends Cell Biol 16:113-20. 2006
    ..This hypothesis explains the impact of COG mutations by postulating that they impair the retrograde flow of resident Golgi proteins needed to maintain normal Golgi structure and function...
  17. ncbi request reprint Irreversible assembly of membrane fusion machines
    Doug Barrick
    Nat Struct Biol 9:78-80. 2002
  18. pmc The COG and COPI complexes interact to control the abundance of GEARs, a subset of Golgi integral membrane proteins
    Toshihiko Oka
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Biol Cell 15:2423-35. 2004
    ..COG and COPI may work in concert to ensure the proper retention or retrieval of a subset of proteins in the Golgi, and COG helps prevent the endoplasmic reticulum accumulation and degradation of some GEARs...
  19. ncbi request reprint Genetic analysis of the subunit organization and function of the conserved oligomeric golgi (COG) complex: studies of COG5- and COG7-deficient mammalian cells
    Toshihiko Oka
    Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 280:32736-45. 2005
    ..Only one or two of the seven Cog1- or Cog2-dependent Golgi membrane proteins called GEARs are also sensitive to Cog5 or Cog7 deficiency, indicating that the COG subunits play distinctive roles in controlling Golgi structure and function...
  20. pmc Structure and stability of the ankyrin domain of the Drosophila Notch receptor
    Mark E Zweifel
    T C Jenkins Department of Biophysics, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Protein Sci 12:2622-32. 2003
    ..The surface substitutions are thus likely to interfere with Notch signaling by disrupting specific Notch-effector interactions and map the sites of these interactions...

Research Grants22

  1. Structural Analysis of Golgi Trafficking Proteins
    FREDERICK HUGHSON; Fiscal Year: 2007
    ..In the final aim, X-ray crystallography will be used to determine the structures of larger COG subassemblies and complexes with other trafficking factors. ..
  2. Structure-Function Analysis of AI-2 Quorum Sensing
    FREDERICK HUGHSON; Fiscal Year: 2007
    ..These studies may also result in the discovery of compounds useful in the development of novel broad-spectrum antibacterial drugs that target quorum sensing. ..
  3. Structural Analysis of Golgi Trafficking Proteins
    FREDERICK HUGHSON; Fiscal Year: 2009
    ..As a consequence, such defects can have pleiotropic effects on the glycosylation and stability of cell surface proteins, leading to human disease. ..
  4. Structure-Function Analysis of AI-2 Quorum Sensing
    Frederick M Hughson; Fiscal Year: 2010
    ..We propose to study quorum sensing in the major human pathogen, Vibrio cholerae, and to identify molecules that target quorum sensing to inhibit virulence. ..
  5. Structural Analysis of Golgi Trafficking Proteins
    Frederick M Hughson; Fiscal Year: 2010
    ..As a consequence, such defects can have pleiotropic effects on the glycosylation and stability of cell surface proteins, leading to human disease. ..
  6. STRUCTURAL ANALYSIS OF INTRACELLULAR TRAFFICKING PROTEIN
    FREDERICK HUGHSON; Fiscal Year: 2002
    ....
  7. STRUCTURE/FUNCTION OF GTC, A NOVEL GOLGI PROTEIN COMPLEX
    FREDERICK HUGHSON; Fiscal Year: 2002
    ..These studies will provide the first insights into the role of this novel protein complex in the process of intracellular protein traffic. ..
  8. Structure-Function Analysis of AI-2 Quorum Sensing
    Frederick M Hughson; Fiscal Year: 2010
    ..We propose to study quorum sensing in the major human pathogen, Vibrio cholerae, and to identify molecules that target quorum sensing to inhibit virulence. ..