Research Topics
Genomes and Genes | Zemer GitaiSummaryAffiliation: Princeton University Country: USA Publications
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Publications
New fluorescence microscopy methods for microbiology: sharper, faster, and quantitativeZemer Gitai
Department of Molecular Biology, Princeton University, Princeton, NJ 08540, USA
Curr Opin Microbiol 12:341-6. 2009..These technical developments are ushering in a new era of using fluorescence microscopy to understand bacterial systems in a detailed, comprehensive, and quantitative manner...
Diversification and specialization of the bacterial cytoskeletonZemer Gitai
Princeton University, Department of Molecular Biology, Washington Road, Princeton, NJ 08544, USA
Curr Opin Cell Biol 19:5-12. 2007..These themes of diversity, species-specificity and crosstalk are emerging as central properties of cytoskeletal biology...- Plasmid segregation: a new class of cytoskeletal proteins emergesZemer Gitai
Princeton University, Department of Molecular Biology, NJ 08544, USA
Curr Biol 16:R133-6. 2006..The discovery that a plasmid-partitioning ATPase forms astral cytoskeletal structures both unveils a new family of cytoskeletal proteins and suggests that cytoskeletal involvement is a universal feature of DNA segregation... - The choreographed dynamics of bacterial chromosomesZemer Gitai
Department of Developmental Biology, Beckman Center, School of Medicine, Stanford University, Stanford, CA 94305, USA
Trends Microbiol 13:221-8. 2005..Here, we review bacterial chromosome dynamics and our understanding of the mechanisms that direct and coordinate them... - The new bacterial cell biology: moving parts and subcellular architectureZemer Gitai
Department of Developmental Biology, Beckman Center, School of Medicine, Stanford University, Stanford, CA 94305, USA
Cell 120:577-86. 2005..The striking conceptual and molecular similarities between prokaryotic and eukaryotic cell biology thus make bacteria powerful model systems for studying fundamental cellular questions... - The bacterial actin MreB rotates, and rotation depends on cell-wall assemblySven van Teeffelen
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
Proc Natl Acad Sci U S A 108:15822-7. 2011..These findings both broaden the view of cytoskeletal motors and deepen our understanding of the physical basis of bacterial morphogenesis... - Imaging-based identification of a critical regulator of FtsZ protofilament curvature in CaulobacterErin D Goley
Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
Mol Cell 39:975-87. 2010..The degree of curvature induced by FzlA depended on the nucleotide bound to FtsZ. Induction of FtsZ curvature by FzlA carries implications for regulating FtsZ function by modulating its superstructure... - MreB actin-mediated segregation of a specific region of a bacterial chromosomeZemer Gitai
Department of Developmental Biology, Beckman Center, School of Medicine, Stanford University, California 94305, USA
Cell 120:329-41. 2005..MreB selectively interacts, directly or indirectly, with origin-proximal regions of the chromosome, arguing that the origin-proximal region segregates via an MreB-dependent mechanism not used by the rest of the chromosome... - The metabolic enzyme CTP synthase forms cytoskeletal filamentsMichael Ingerson-Mahar
Department of Molecular Biology, Princeton University, Lewis Thomas Labs, Washington Road, Princeton, NJ 08544, USA
Nat Cell Biol 12:739-46. 2010..These results implicate CtpS as a novel bifunctional member of the bacterial cytoskeleton and suggest that localization and polymerization may be important properties of metabolic enzymes... - Caulobacter chromosome segregation is an ordered multistep processConrad W Shebelut
Department of Molecular Biology, Princeton University, Lewis Thomas Laboratory, Princeton, NJ 08544, USA
Proc Natl Acad Sci U S A 107:14194-8. 2010..We discuss how the multistep view of bacterial chromosome segregation can help to explain and reconcile outstanding puzzles and frame future investigation... - Quantitative genome-scale analysis of protein localization in an asymmetric bacteriumJohn N Werner
Department of Molecular Biology, Princeton University, Princeton, NJ 08540, USA
Proc Natl Acad Sci U S A 106:7858-63. 2009.... - Bacterial cell division spirals into controlZemer Gitai
Department of Developmental Biology, Stanford University School of Medicine, Beckman Center B351, Stanford, CA 94305-5427, USA
Proc Natl Acad Sci U S A 100:7423-4. 2003 
A growing family: the expanding universe of the bacterial cytoskeletonMichael Ingerson-Mahar
Department of Molecular Biology, Princeton University, Princeton, NJ, USA
FEMS Microbiol Rev 36:256-66. 2012..Here, we summarize the recent explosion in the identification of new members of the bacterial cytoskeleton and describe a hypothesis for the evolution of the cytoskeleton from self-assembling enzymes...- Self-assembling enzymes and the origins of the cytoskeletonRachael M Barry
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, United States
Curr Opin Microbiol 14:704-11. 2011..The behaviors of these enzymes suggest that some modern cytoskeletal proteins may have evolved from dual-role proteins with catalytic and structural functions... - Single molecules of the bacterial actin MreB undergo directed treadmilling motion in Caulobacter crescentusSo Yeon Kim
Department of Chemistry, Stanford University, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 103:10929-34. 2006..Thus, MreB, like actin, exhibits treadmilling behavior in vivo, and the long MreB structures that have been visualized in multiple bacterial species seem to represent bundles of short filaments that lack a uniform global polarity... - Dynamic polar sequestration of excess MurG may regulate enzymatic functionAllison M Michaelis
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
J Bacteriol 192:4597-605. 2010..Together, our results imply that inactive MurG is dynamically sequestered at the cell poles and that prokaryotes can thus utilize subcellular localization as a mechanism for negatively regulating enzymatic activity... - The structure and function of bacterial actin homologsJoshua W Shaevitz
Department of Physics, Princeton University, Princeton, New Jersey 08544, USA
Cold Spring Harb Perspect Biol 2:a000364. 2010..We also discuss the outstanding puzzles in the field and possible directions where this fast-developing area may progress in the future... - Growth conditions regulate the requirements for Caulobacter chromosome segregationConrad W Shebelut
Department of Molecular Biology, Princeton University, Washington Rd, Princeton, NJ 08544, USA
J Bacteriol 191:1097-100. 2009..Our results also implicate ParAB as important segregation determinants, suggesting that multiple distinct mechanisms can mediate Caulobacter chromosome segregation and that their relative contributions can be environmentally regulated... - PSICIC: noise and asymmetry in bacterial division revealed by computational image analysis at sub-pixel resolutionJonathan M Guberman
Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
PLoS Comput Biol 4:e1000233. 2008.... - Image analysis in fluorescence microscopy: bacterial dynamics as a case studySven van Teeffelen
Princeton University, Department of Molecular Biology, Princeton, NJ, USA
Bioessays 34:427-36. 2012..Thus, image analysis is not only a toolkit to be applied to new images but also an integral part of the design and implementation of a microscopy experiment... - High-throughput screening of bacterial protein localizationJohn N Werner
Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA
Methods Enzymol 471:185-204. 2010..The cloning, imaging, and image analysis techniques described here are applicable to any organism of interest... - An actin-like gene can determine cell polarity in bacteriaZemer Gitai
Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 101:8643-8. 2004..We propose that the molecular polarity inherent in an actin-like filament is translated into a mechanism for directing global cell polarity... - Flow directs surface-attached bacteria to twitch upstreamYi Shen
Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, New Jersey, USA
Biophys J 103:146-51. 2012..This directed upstream motility could be beneficial in environments where flow is present, allowing bacteria to colonize environments that cannot be reached by other surface-attached bacteria... - Surface association and the MreB cytoskeleton regulate pilus production, localization and function in Pseudomonas aeruginosaKimberly N Cowles
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
Mol Microbiol 76:1411-26. 2010..aeruginosa pili and demonstrate that protein localization may represent an important aspect of virulence factor regulation in bacterial pathogens... - Two independent spiral structures control cell shape in CaulobacterNatalie A Dye
Department of Biochemistry, Stanford University, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 102:18608-13. 2005.... - BapE DNA endonuclease induces an apoptotic-like response to DNA damage in CaulobacterJulia Bos
Department of Molecular Biology, Princeton University, Princeton, NJ 08544
Proc Natl Acad Sci U S A 109:18096-101. 2012.... - Cell shape and cell-wall organization in Gram-negative bacteriaKerwyn Casey Huang
Department of Molecular Biology, Princeton University, Washington Road, Princeton, NJ 08544 1014, USA
Proc Natl Acad Sci U S A 105:19282-7. 2008.... - The small protein MbiA interacts with MreB and modulates cell shape in Caulobacter crescentusAnastasiya A Yakhnina
Department of Molecular Biology, Princeton University, Lewis Thomas Laboratory, Princeton, NJ 08544, USA
Mol Microbiol 85:1090-104. 2012.... - Location and architecture of the Caulobacter crescentus chemoreceptor arrayAriane Briegel
Division of Biology, California Institute of Technology, 1200 E California Blvd, Pasadena, CA 91125, USA
Mol Microbiol 69:30-41. 2008..Based on this model for the arrangement of receptors, there are between one and two thousand receptors per array...