Lynn Enquist

Summary

Affiliation: Princeton University
Country: USA

Publications

  1. pmc UL54-null pseudorabies virus is attenuated in mice but productively infects cells in culture
    Jennifer A Schwartz
    Department of Microbiology, Columbia University, 701 W 168th St, New York, NY 10032, USA
    J Virol 80:769-84. 2006
  2. pmc Targeting of pseudorabies virus structural proteins to axons requires association of the viral Us9 protein with lipid rafts
    Mathew G Lyman
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
    PLoS Pathog 4:e1000065. 2008
  3. pmc Imaging the transport dynamics of single alphaherpesvirus particles in intact peripheral nervous system explants from infected mice
    Andrea E Granstedt
    Department of Molecular Biology, Princeton University, Princeton, NJ, USA
    MBio 4:e00358-13. 2013
  4. pmc A microfluidic chamber for analysis of neuron-to-cell spread and axonal transport of an alpha-herpesvirus
    Wendy W Liu
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
    PLoS ONE 3:e2382. 2008
  5. pmc Fluorescence-based monitoring of in vivo neural activity using a circuit-tracing pseudorabies virus
    Andrea E Granstedt
    Department of Molecular Biology, Neuroscience Institute, Princeton University, Princeton, New Jersey, United States of America
    PLoS ONE 4:e6923. 2009
  6. pmc Pseudorabies virus infection alters neuronal activity and connectivity in vitro
    Kelly M McCarthy
    Department of Molecular Biology and Princeton Neuroscience Institute, Princeton University, Princeton, New Jersey, USA
    PLoS Pathog 5:e1000640. 2009
  7. pmc Herpesviruses carrying a Brainbow cassette reveal replication and expression of limited numbers of incoming genomes
    Oren Kobiler
    Department of Molecular Biology and the Princeton Neuroscience Institute, Princeton University, Princeton, New Jersey 08544, USA
    Nat Commun 1:146. 2010
  8. pmc A wide extent of inter-strain diversity in virulent and vaccine strains of alphaherpesviruses
    Moriah L Szpara
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
    PLoS Pathog 7:e1002282. 2011
  9. pmc Role of Us9 phosphorylation in axonal sorting and anterograde transport of pseudorabies virus
    Radomir Kratchmarov
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
    PLoS ONE 8:e58776. 2013
  10. pmc Directional spread of alphaherpesviruses in the nervous system
    Tal Kramer
    Princeton University, Department of Molecular Biology, Princeton, NJ 08544, USA
    Viruses 5:678-707. 2013

Research Grants

Collaborators

Detail Information

Publications32

  1. pmc UL54-null pseudorabies virus is attenuated in mice but productively infects cells in culture
    Jennifer A Schwartz
    Department of Microbiology, Columbia University, 701 W 168th St, New York, NY 10032, USA
    J Virol 80:769-84. 2006
    ..Animals infected with vJSdelta54 survive twice as long as animals infected with wild-type virus, and this results in delayed accumulation of virus-specific antigens in skin, dorsal root ganglia, and spinal cord tissues...
  2. pmc Targeting of pseudorabies virus structural proteins to axons requires association of the viral Us9 protein with lipid rafts
    Mathew G Lyman
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
    PLoS Pathog 4:e1000065. 2008
    ....
  3. pmc Imaging the transport dynamics of single alphaherpesvirus particles in intact peripheral nervous system explants from infected mice
    Andrea E Granstedt
    Department of Molecular Biology, Princeton University, Princeton, NJ, USA
    MBio 4:e00358-13. 2013
    ..Importantly, this system enabled us to address a fundamental biological question about the amplification of a herpesvirus infection in a peripheral nervous system circuit...
  4. pmc A microfluidic chamber for analysis of neuron-to-cell spread and axonal transport of an alpha-herpesvirus
    Wendy W Liu
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
    PLoS ONE 3:e2382. 2008
    ..Using PRV infection of cultured PNS neurons, we demonstrate that the microfluidic chamber recapitulates all known facets of neuron-to-cell spread demonstrated in animals and other compartmented cell systems...
  5. pmc Fluorescence-based monitoring of in vivo neural activity using a circuit-tracing pseudorabies virus
    Andrea E Granstedt
    Department of Molecular Biology, Neuroscience Institute, Princeton University, Princeton, New Jersey, United States of America
    PLoS ONE 4:e6923. 2009
    ..Our work establishes in vivo investigation of physiological neuronal circuit activity and subsequent effects of infection with single cell resolution...
  6. pmc Pseudorabies virus infection alters neuronal activity and connectivity in vitro
    Kelly M McCarthy
    Department of Molecular Biology and Princeton Neuroscience Institute, Princeton University, Princeton, New Jersey, USA
    PLoS Pathog 5:e1000640. 2009
    ..We suggest that formation of fusion pores between infected neurons results in electrical coupling and elevated firing rates, and that these processes may contribute to the altered neural function seen in PRV-infected animals...
  7. pmc Herpesviruses carrying a Brainbow cassette reveal replication and expression of limited numbers of incoming genomes
    Oren Kobiler
    Department of Molecular Biology and the Princeton Neuroscience Institute, Princeton University, Princeton, New Jersey 08544, USA
    Nat Commun 1:146. 2010
    ..This finite limit on the number of viral genomes that can be expressed is an intrinsic property of the infected cell and may be influenced by viral and cellular factors...
  8. pmc A wide extent of inter-strain diversity in virulent and vaccine strains of alphaherpesviruses
    Moriah L Szpara
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
    PLoS Pathog 7:e1002282. 2011
    ..These data add to growing evidence that even plaque-purified stocks of stable DNA viruses exhibit limited sequence heterogeneity, which likely seeds future strain evolution...
  9. pmc Role of Us9 phosphorylation in axonal sorting and anterograde transport of pseudorabies virus
    Radomir Kratchmarov
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
    PLoS ONE 8:e58776. 2013
    ..Taken together, our findings indicate that, while not essential, Us9 phosphorylation enhances Us9-Kif1A-based transport of virions in axons to modulate the overall efficiency of long-distance anterograde spread of infection...
  10. pmc Directional spread of alphaherpesviruses in the nervous system
    Tal Kramer
    Princeton University, Department of Molecular Biology, Princeton, NJ 08544, USA
    Viruses 5:678-707. 2013
    ..We focus on the molecular events that mediate long distance directional transport of viral particles in neurons during entry and egress...
  11. pmc Compartmented neuron cultures for directional infection by alpha herpesviruses
    Dusica Curanovic
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA
    Curr Protoc Cell Biol . 2009
    ..This allows for quantification of viral spread, and unambiguous interpretation of immunofluorescence and electron microscopy images...
  12. pmc Human cytomegalovirus UL99-encoded pp28 is required for the cytoplasmic envelopment of tegument-associated capsids
    Maria C Silva
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544 1014, USA
    J Virol 77:10594-605. 2003
    ..We conclude that pp28 is required for the final envelopment of the human cytomegalovirus virion in the cytoplasm...
  13. ncbi request reprint Recent advances in the use of neurotropic viruses for circuit analysis
    Lynn W Enquist
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Curr Opin Neurobiol 13:603-6. 2003
    ....
  14. pmc Alpha-herpesvirus infection induces the formation of nuclear actin filaments
    Becket Feierbach
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA
    PLoS Pathog 2:e85. 2006
    ..Our results suggest a mechanism by which assembly domains are organized within infected cells and provide insight into how the viral infectious cycle and host actin cytoskeleton are integrated to promote the infection process...
  15. pmc Transcriptome signature of virulent and attenuated pseudorabies virus-infected rodent brain
    Christina Paulus
    Department of Molecular Biology, Princeton University, Princeton, NJ08544 1014, USA
    J Virol 80:1773-86. 2006
    ..The differentially regulated genes reported here are likely to include potential diagnostic and therapeutic targets for viral encephalitides and other neurodegenerative or neuroinflammatory diseases...
  16. pmc Herpes simplex virus type 1 glycoprotein e is required for axonal localization of capsid, tegument, and membrane glycoproteins
    Fushan Wang
    Department of Medicine, Division of Infectious Diseases, 502 Johnson Pavilion, University of Pennsylvania, Philadelphia, PA 19104 6073, USA
    J Virol 79:13362-72. 2005
    ..The requirement for gE in axonal targeting and retrograde spread highlights intriguing similarities and differences between HSV-1 and pseudorabies virus gE...
  17. ncbi request reprint Intravitreal injection of the attenuated pseudorabies virus PRV Bartha results in infection of the hamster suprachiasmatic nucleus only by retrograde transsynaptic transport via autonomic circuits
    Gary E Pickard
    Department of Anatomy, Colorado State University, Fort Collins, Colorado 80523, USA
    J Neurosci 22:2701-10. 2002
    ..These results, taken together with other genetic data, indicate that the mutations in PRV Bartha render the virus incapable of anterograde transport. PRV Bartha is thus a retrograde transsynaptic marker in the CNS...
  18. ncbi request reprint Functional integration of adult-born neurons
    Marie Carlen
    Department of Cell and Molecular Biology, Medical Nobel Institute, Stockholm, Sweden
    Curr Biol 12:606-8. 2002
    ..Here, we use virus-based transsynaptic neuronal tracing and c-Fos mapping of odor-induced neuronal activity to demonstrate that neurons generated in the adult functionally integrate into the synaptic circuitry of the brain...
  19. pmc Physical interaction between envelope glycoproteins E and M of pseudorabies virus and the major tegument protein UL49
    Walter Fuchs
    Institute of Molecular Biology, Friedrich Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, D 17498 Insel Riems, Germany
    J Virol 76:8208-17. 2002
    ..In summary, we describe specific interactions between herpesvirus envelope and tegument proteins which may play a role in secondary envelopment during herpesvirus virion maturation...
  20. ncbi request reprint Pseudorabies virus-induced expression of nitric oxide synthase isoforms
    Faridis Serrano
    Department of Neuroscience, University of Pittsburgh, 446 Crawford Hall, 15260, Pittsburgh, PA, USA
    Physiol Behav 77:557-63. 2002
    ..Collectively, these data suggest functionally diverse roles for NO in the brain response to PRV neuronal infection...
  21. ncbi request reprint Brainstem substrates of sympatho-motor circuitry identified using trans-synaptic tracing with pseudorabies virus recombinants
    Ilan A Kerman
    Department of Neuroscience University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Neurosci 23:4657-66. 2003
    ..These neurons are candidates for relaying central command signals to the spinal cord...
  22. ncbi request reprint Suprachiasmatic nucleus input to autonomic circuits identified by retrograde transsynaptic transport of pseudorabies virus from the eye
    Cynthia A Smeraski
    Department of Biomedical Sciences, Section of Anatomy and Neurobiology, Colorado State University, Fort Collins, Colorado 80523, USA
    J Comp Neurol 471:298-313. 2004
    ..The results indicate that in the rat, as in the hamster, SCN infection after intraocular PRV152 inoculation is by retrograde transsynaptic transport via autonomic pathways to the eye...
  23. pmc Complete, annotated sequence of the pseudorabies virus genome
    Barbara G Klupp
    Institute of Molecular Biology, Friedrich Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, D 17493 Greifswald Insel Riems, Germany
    J Virol 78:424-40. 2004
    ..Many of these features are conserved in the alphaherpesvirus subfamily and have important implications for gene array analyses...
  24. ncbi request reprint Break ins and break outs: viral interactions with the cytoskeleton of Mammalian cells
    Gregory A Smith
    Department of Microbiology Immunology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    Annu Rev Cell Dev Biol 18:135-61. 2002
    ..Understanding these interactions, and their orchestration during viral infections, is providing unexpected insights into basic virology, viral pathogenesis, and the biology of the cytoskeleton...
  25. pmc In vivo egress of an alphaherpesvirus from axons
    Mark J Tomishima
    Department of Molecular Biology, Princeton University, New Jersey 08544, USA
    J Virol 76:8310-7. 2002
    ..Taking these together with previous data, we suggest a model of viral egress from neurons that unifies previous apparently contradictory data...
  26. ncbi request reprint The pseudorabies virus US3 protein kinase possesses anti-apoptotic activity that protects cells from apoptosis during infection and after treatment with sorbitol or staurosporine
    Kristin Geenen
    Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Salisbury laan 133, 9820 Merelbeke, East Flanders, Belgium
    Virology 331:144-50. 2005
    ....
  27. pmc A tyrosine-based motif in the cytoplasmic tail of pseudorabies virus glycoprotein B is important for both antibody-induced internalization of viral glycoproteins and efficient cell-to-cell spread
    Herman W Favoreel
    Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
    J Virol 76:6845-51. 2002
    ..Mutating the dileucine motif in the gB tail led to an increased cell-to-cell spread of the virus and the formation of large syncytia...
  28. ncbi request reprint Local retinal circuits of melanopsin-containing ganglion cells identified by transsynaptic viral tracing
    Tim James Viney
    Neural Circuit Laboratories, Friedrich Miescher Institute, Maulbeerstrasse 66, Basel 4058, Switzerland
    Curr Biol 17:981-8. 2007
    ..Second, we show evidence that dopaminergic interplexiform cells are synaptically connected to ipRGCs. The latter finding provides a circuitry link between light-dark adaptation and ipRGC function...
  29. ncbi request reprint New developments in tracing neural circuits with herpesviruses
    C Kay Song
    Department of Biology, Neurobiology and Behavior Program, Georgia State University, 24 Peachtree Center Ave NE, Atlanta, GA 30302 4010, USA
    Virus Res 111:235-49. 2005
    ..We provide examples of these combined application techniques within the context of an animal model to study the naturally occurring reversal of seasonal obesity in Siberian hamsters...
  30. pmc Construction of a self-excisable bacterial artificial chromosome containing the human cytomegalovirus genome and mutagenesis of the diploid TRL/IRL13 gene
    Dong Yu
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544 1014, USA
    J Virol 76:2316-28. 2002
    ..This view is consistent with the presence of both TRL13 and -14 on all mRNAs that we have mapped from this region, an organization that would include the much longer strain Toledo TRL13 open reading frame on the mRNAs...

Research Grants19

  1. Neuronal Spread of Herpesvirus Infection
    Lynn W Enquist; Fiscal Year: 2010
    ..These neural tracers would be powerful tools to elucidate brain micro-circuitry, providing a better understanding of nervous system functions. ..
  2. GENETIC ANALYSIS OF HERPESVIRUS TROPISM AND VIRULENCE
    Lynn Enquist; Fiscal Year: 2007
    ..The goal is to identify and characterize the PRV genes whose products stimulate abnormal and persistent neuronal firing of peripheral nervous system neurons. ..
  3. Viruses And Cells Gordon Conference
    Lynn Enquist; Fiscal Year: 2003
    ..This conference is one of the few smaller meetings in virology that encompasses a broad view of the field. Funding is requested to support the registration and travel of graduate students and postdoctoral fellows to this meeting. ..
  4. GENERTIC ANALYSIS OF HERPESVIRUS TROPISM AND VIRULENCE
    Lynn Enquist; Fiscal Year: 2003
    ..abstract_text> ..
  5. GENETIC ANALYSIS OF HERPESVIRUS TROPISM AND VIRULENCE
    Lynn Enquist; Fiscal Year: 1999
    ..A clear genetic definition of functional domains in the gE and gI proteins will give significant insight into molecular mechanisms of tropism and pathogenesis. ..
  6. GENERTIC ANALYSIS OF HERPESVIRUS TROPISM AND VIRULENCE
    Lynn Enquist; Fiscal Year: 2000
    ..abstract_text> ..
  7. Neuronal Spread of Herpesvirus Infection
    Lynn W Enquist; Fiscal Year: 2010
    ..These neural tracers would be powerful tools to elucidate brain micro-circuitry, providing a better understanding of nervous system functions. ..
  8. Neuronal Spread of Herpesvirus Infection
    Lynn Enquist; Fiscal Year: 2009
    ..These neural tracers would be powerful tools to elucidate brain micro-circuitry, providing a better understanding of nervous system functions. ..
  9. GENERTIC ANALYSIS OF HERPESVIRUS TROPISM AND VIRULENCE
    Lynn Enquist; Fiscal Year: 2003
    ..abstract_text> ..
  10. GENERTIC ANALYSIS OF HERPESVIRUS TROPISM AND VIRULENCE
    Lynn Enquist; Fiscal Year: 2004
    ..abstract_text> ..
  11. GENERTIC ANALYSIS OF HERPESVIRUS TROPISM AND VIRULENCE
    Lynn Enquist; Fiscal Year: 2002
    ..abstract_text> ..
  12. GENERTIC ANALYSIS OF HERPESVIRUS TROPISM AND VIRULENCE
    Lynn Enquist; Fiscal Year: 2001
    ..abstract_text> ..
  13. GENERTIC ANALYSIS OF HERPESVIRUS TROPISM AND VIRULENCE
    Lynn Enquist; Fiscal Year: 2000
    ..abstract_text> ..