Robert Austin

Summary

Affiliation: Princeton University
Country: USA

Publications

  1. ncbi request reprint Critical particle size for fractionation by deterministic lateral displacement
    David W Inglis
    Princeton Institute for the Science and Technology of Materials, PRISM Princeton University, Princeton, NJ 08544, USA
    Lab Chip 6:655-8. 2006
  2. pmc The Sackler Colloquium on promises and perils in nanotechnology for medicine
    Robert H Austin
    Department of Physics, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 105:17217-21. 2008
  3. doi request reprint Nanofluidics: a fork in the nano-road
    Robert Austin
    Department of Physics, Princeton University, Princeton, New Jersey 08544, USA
    Nat Nanotechnol 2:79-80. 2007
  4. doi request reprint An introduction to micro-ecology patches
    Robert H Austin
    Department of Physics, Princeton University, Princeton, NJ 08544, USA
    Chem Soc Rev 39:1049-59. 2010
  5. ncbi request reprint Of spherical cows, cloudy crystal balls, and proteins
    Robert H Austin
    Department of Physics, Princeton University, Princeton, NJ 08544, USA
    Biochem Biophys Res Commun 312:215-21. 2003
  6. pmc Tilting after Dutch windmills: probably no long-lived Davydov solitons in proteins
    Robert H Austin
    Department of Physics, Princeton University, Princeton, NJ, USA
    J Biol Phys 35:91-101. 2009
  7. ncbi request reprint Single sub-20 nm wide, centimeter-long nanofluidic channel fabricated by novel nanoimprint mold fabrication and direct imprinting
    Xiaogan Liang
    NanoStructure Laboratory, Department of Electrical Engineering, Princeton University, Princeton, NJ 08544, USA
    Nano Lett 7:3774-80. 2007
  8. pmc Burst analysis spectroscopy: a versatile single-particle approach for studying distributions of protein aggregates and fluorescent assemblies
    Jason Puchalla
    Department of Physics, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 105:14400-5. 2008
  9. pmc Restriction mapping in nanofluidic devices
    Robert Riehn
    Departments of Physics, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 102:10012-6. 2005
  10. ncbi request reprint In vivo and scanning electron microscopy imaging of up-converting nanophosphors in Caenorhabditis elegans
    Shuang Fang Lim
    Department of Physics, Princeton University, Princeton, New Jersey 08544, Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA
    Nano Lett 6:169-74. 2006

Collaborators

Detail Information

Publications40

  1. ncbi request reprint Critical particle size for fractionation by deterministic lateral displacement
    David W Inglis
    Princeton Institute for the Science and Technology of Materials, PRISM Princeton University, Princeton, NJ 08544, USA
    Lab Chip 6:655-8. 2006
    ..In general the critical particle diameter is much smaller than the gap, which prevents clogging. The model is supported by data with particles from 2.3 to 22 microm...
  2. pmc The Sackler Colloquium on promises and perils in nanotechnology for medicine
    Robert H Austin
    Department of Physics, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 105:17217-21. 2008
    ..The papers collected here represent a cross-section of these issues. As an example, we present our own work on nano-upconversion phosphors as an example of this new class of nanomaterials with potential use in medicine and biology...
  3. doi request reprint Nanofluidics: a fork in the nano-road
    Robert Austin
    Department of Physics, Princeton University, Princeton, New Jersey 08544, USA
    Nat Nanotechnol 2:79-80. 2007
  4. doi request reprint An introduction to micro-ecology patches
    Robert H Austin
    Department of Physics, Princeton University, Princeton, NJ 08544, USA
    Chem Soc Rev 39:1049-59. 2010
    ..In this tutorial review we discuss how simplified micro-ecologies constructed using tools of micro and nanofabrication techniques offer some idea of how physical principles and analysis can address the issue of complex ecology dynamics...
  5. ncbi request reprint Of spherical cows, cloudy crystal balls, and proteins
    Robert H Austin
    Department of Physics, Princeton University, Princeton, NJ 08544, USA
    Biochem Biophys Res Commun 312:215-21. 2003
    ..We discuss how the early work of Irwin C. "Gunny" Gunsalus with Hans Frauenfelder and Peter Debrunner led to insights into the picosecond dynamics of proteins, the initial functionally important motions...
  6. pmc Tilting after Dutch windmills: probably no long-lived Davydov solitons in proteins
    Robert H Austin
    Department of Physics, Princeton University, Princeton, NJ, USA
    J Biol Phys 35:91-101. 2009
    ..Alas, after much effort, we believe the data show that there is no long-lived Davydov soliton, at least in myoglobin...
  7. ncbi request reprint Single sub-20 nm wide, centimeter-long nanofluidic channel fabricated by novel nanoimprint mold fabrication and direct imprinting
    Xiaogan Liang
    NanoStructure Laboratory, Department of Electrical Engineering, Princeton University, Princeton, NJ 08544, USA
    Nano Lett 7:3774-80. 2007
    ..The centimeter-long channel continuity is verified by flowing fluorescent dye-stained water and stretching and transporting DNAs. The 18 by 20 nm channel cross-section was confirmed by measuring the liquid conductance in the channel...
  8. pmc Burst analysis spectroscopy: a versatile single-particle approach for studying distributions of protein aggregates and fluorescent assemblies
    Jason Puchalla
    Department of Physics, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 105:14400-5. 2008
    ..We find that the assembly kinetics of both proteins display complex multimodal behavior not readily quantifiable with other methods...
  9. pmc Restriction mapping in nanofluidic devices
    Robert Riehn
    Departments of Physics, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 102:10012-6. 2005
    ..We have successfully used the restriction enzymes SmaI, SacI, and PacI, and have been able to measure the positions of restriction sites with a precision of approximately 1.5 kbp in 1 min using single DNA molecules...
  10. ncbi request reprint In vivo and scanning electron microscopy imaging of up-converting nanophosphors in Caenorhabditis elegans
    Shuang Fang Lim
    Department of Physics, Princeton University, Princeton, New Jersey 08544, Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA
    Nano Lett 6:169-74. 2006
    ....
  11. ncbi request reprint Wetting micro- and nanofluidic devices using supercritical water
    Robert Riehn
    Department of Physics, Princeton University, Princeton, New Jersey 08544, USA
    Anal Chem 78:5933-4. 2006
    ..We demonstrate wetting of micro- and nanostructures in a fused-silica device with only a single inlet. The process is low-cost, fast, safe, and very reliable...
  12. ncbi request reprint Single molecule correlation spectroscopy in continuous flow mixers with zero-mode waveguides
    David Liao
    Department of Physics, Princeton University, Princeton, NJ 08544, USA
    Opt Express 16:10077-90. 2008
    ..Thus zero-mode waveguides make FCS suitable for direct kinetics measurements in rapid continuous flow...
  13. ncbi request reprint A nanofluidic railroad switch for DNA
    Robert Riehn
    Department of Physics, Princeton University, Princeton, New Jersey 08544, USA
    Nano Lett 6:1973-6. 2006
    ..We explain the results in terms of an energy landscape for polyelectrolytes that consists of entropic and dielectrophoretic contributions and whose strength and sign can be tuned by changing the ac field strength...
  14. doi request reprint Sub-10 nm self-enclosed self-limited nanofluidic channel arrays
    Qiangfei Xia
    NanoStructure Laboratory, Department of Electrical Engineering, Princeton University, Princeton, New Jersey 08544, USA
    Nano Lett 8:3830-3. 2008
    ..We demonstrate that 100 nm wide Si trenches can be sealed and shrunk to 9 nm wide and that lambda-phage DNA molecules can be effectively stretched by the channels...
  15. pmc Hydrodynamic metamaterials: microfabricated arrays to steer, refract, and focus streams of biomaterials
    Keith J Morton
    Departments of Electrical Engineering and Physics, Princeton University, Princeton, NJ 08544 1014, USA
    Proc Natl Acad Sci U S A 105:7434-8. 2008
    ..The unusual aspects of these modular, microfluidic metamaterials form a rich design toolkit for mixing, separating, and analyzing cells and functional beads on-chip...
  16. ncbi request reprint A DNA prism for high-speed continuous fractionation of large DNA molecules
    Lotien Richard Huang
    Center for Photonics and Optoelectronic Materials POEM, Princeton University, Princeton, NJ 08544, USA
    Nat Biotechnol 20:1048-51. 2002
    ..We demonstrate the robustness of the device by using it to separate large DNA inserts prepared from bacterial artificial chromosomes, a widely used DNA source for most genomics projects...
  17. pmc Electrodeless dielectrophoresis of single- and double-stranded DNA
    Chia Fu Chou
    Department of Physics, Princeton University, New Jersey 08544, USA
    Biophys J 83:2170-9. 2002
    ..A possible mechanism for DNA polarization in ionic solution is discussed based on the frequency, viscosity, and field dependence of the observed trapping force...
  18. ncbi request reprint Nanofabricated upconversion nanoparticles for photodynamic therapy
    Baris Ungun
    Department of Chemical Engineering, Princeton University, Princeton, NJ, USA
    Opt Express 17:80-6. 2009
    ..We show that these CNPs produce millimolar amounts of singlet oxygen at NIR intensities far less than other two-photon techniques...
  19. ncbi request reprint Tilted brownian ratchet for DNA analysis
    Lotien Richard Huang
    Center for Photonics and Optoelectronic Materials POEM, Departments of Electrical Engineering, Molecular Biology, and Physics, Princeton University, Princeton, New Jersey 08544, USA
    Anal Chem 75:6963-7. 2003
    ..8, using a 12-mm-long array. The Brownian ratchet arrays are not limited to DNA separation, but can, in principle, be used for any particle in this size range...
  20. ncbi request reprint Micro- and nanofluidics for DNA analysis
    Jonas O Tegenfeldt
    Department of Molecular Biology, Princeton University, Princeton, NJ, USA
    Anal Bioanal Chem 378:1678-92. 2004
    ....
  21. ncbi request reprint Continuous particle separation through deterministic lateral displacement
    Lotien Richard Huang
    Department of Electrical Engineering, Princeton Institute for the Science and Technology of Materials PRISM, Princeton University, Princeton, NJ 08544, USA
    Science 304:987-90. 2004
    ..Bacterial artificial chromosomes could be separated in 10 minutes with a resolution of approximately 12%...
  22. ncbi request reprint Single molecule measurements of repressor protein 1D diffusion on DNA
    Y M Wang
    Department of Physics, Princeton University, Princeton, New Jersey 08544, USA
    Phys Rev Lett 97:048302. 2006
    ....
  23. doi request reprint Upconverting nanophosphors for bioimaging
    Shuang Fang Lim
    Department of MAE, Princeton University, Princeton, NJ 08544, USA
    Nanotechnology 20:405701. 2009
    ..The amine modified UCNPs show specific surface immobilization onto patterned gold surfaces. Finally, the low toxicity of the UCNPs is verified by testing on the multi-cellular C. elegans nematode...
  24. pmc A microfluidic device for continuous cancer cell culture and passage with hydrodynamic forces
    Liyu Liu
    Dept of Physics, Princeton University, NJ, USA
    Lab Chip 10:1807-13. 2010
    ..The chip performs 9 passages during 30 days of continuous culture and shows promise as a durable design suitable for long-term cell output...
  25. ncbi request reprint Deterministic microfluidic ratchet
    Kevin Loutherback
    Princeton Institute for Science and Technology of Materials, Princeton, New Jersey, USA
    Phys Rev Lett 102:045301. 2009
    ..Diffusion plays no role in this ratchet, and hence the device parameters presented here can be scaled up to high rates of flow, of clear importance in separation technologies...
  26. pmc Single-molecule studies of repressor-DNA interactions show long-range interactions
    Y M Wang
    Departments of Physics, Molecular Biology, and Electrical Engineering, Princeton University, Princeton, NJ 08544
    Proc Natl Acad Sci U S A 102:9796-801. 2005
    ..5% of the available sites. This low occupancy level suggests that the repressors influence each other even when they are widely separated, at distances on the order of 200 nm, or several DNA persistence lengths...
  27. ncbi request reprint Role of molecular size in ratchet fractionation
    Lotien Richard Huang
    Center for Photonics and Optoelectronic Materials POEM, Department of Electrical Engineering, Princeton University, Princeton, New Jersey 08544, USA
    Phys Rev Lett 89:178301. 2002
    ..We demonstrate this effect using a new technological approach to create very straight and narrow injection bands in such arrays, and completely resolve bands of DNA of lengths 48,500 and 16,7000 base pairs...
  28. ncbi request reprint Determining blood cell size using microfluidic hydrodynamics
    David W Inglis
    Princeton Institute for the Science and Technology of Materials PRISM, Department of Electrical Engineering, Princeton University, Princeton, NJ 08544, USA
    J Immunol Methods 329:151-6. 2008
    ..Together the results demonstrate a microfluidic device that performs some of the measurement and separation tasks of a flow cytometer but at a potentially lower cost and complexity...
  29. pmc Bacterial metapopulations in nanofabricated landscapes
    Juan E Keymer
    Department of Physics, Princeton University, Princeton, NJ 08544 1014, USA
    Proc Natl Acad Sci U S A 103:17290-5. 2006
    ..Our results illustrate the potential lying at the interface between nanoscale biophysics and landscape evolutionary ecology...
  30. ncbi request reprint Excited-state lifetimes of far-infrared collective modes in proteins
    Aihua Xie
    Department of Physics, Oklahoma State University, Stillwater, OK 74078, USA
    Phys Rev Lett 88:018102. 2002
    ....
  31. ncbi request reprint Statics and dynamics of single DNA molecules confined in nanochannels
    Walter Reisner
    Physics Department, Princeton University, New Jersey 08544, USA
    Phys Rev Lett 94:196101. 2005
    ..Here we present measurements of DNA extended in nanochannels and show that below a critical width roughly twice the persistence length there is a crossover in the polymer physics...
  32. ncbi request reprint Picosecond thermometer in the amide I band of myoglobin
    Robert H Austin
    Department of Physics, Princeton University, Princeton, New Jersey 08544, USA
    Phys Rev Lett 94:128101. 2005
    ..We determine that vibrational energy flow to the hydration shell from the amide I takes approximately 20 ps to occur...
  33. ncbi request reprint Bacterial chromosome extraction and isolation
    Christelle Prinz
    Department of Physics, Princeton University, Princeton, NJ 08544, USA
    Lab Chip 2:207-12. 2002
    ..coli chromosome. We characterize the conditions needed for efficient lysis of the cells, and conditions needed for the dielectrophoretic trapping of the chromatin without the trapping of cytoplasmic proteins...
  34. ncbi request reprint Collective escape of chemotactic swimmers through microscopic ratchets
    Guillaume Lambert
    Department of Physics, Princeton University, Princeton, New Jersey 08544, USA
    Phys Rev Lett 104:168102. 2010
    ..A transition between pure rectification and chemotaxis-driven collective motion is predicted from theoretical models, and is observed experimentally as the initial inoculation density is varied...
  35. doi request reprint Crossing microfluidic streamlines to lyse, label and wash cells
    Keith J Morton
    Princeton Institute for the Science and Technology of Materials, Department of Electrical Engineering, Princeton University, NJ, USA
    Lab Chip 8:1448-53. 2008
    ..The washing capabilities of this method are particularly valuable because they allow many analytical or treatment procedures to be cascaded on a single device while still effectively isolating their reagents from cross-contamination...
  36. pmc From the Cover: The dynamics of genomic-length DNA molecules in 100-nm channels
    Jonas O Tegenfeldt
    Department of Physics, Lund University, SE 221 00 Lund, Sweden
    Proc Natl Acad Sci U S A 101:10979-83. 2004
    ..This statistical analysis allows us to measure the extension of lambda DNA multimers with a 130-nm SD in 1 min...
  37. doi request reprint Microfluidic device for label-free measurement of platelet activation
    David W Inglis
    Department of Physics, Macquarie University, Sydney, NSW 2109, Australia
    Lab Chip 8:925-31. 2008
    ..We infer that the well known morphological changes that occur during activation are causing the observed increase in size...
  38. pmc Parallel microchannel-based measurements of individual erythrocyte areas and volumes
    Sean C Gifford
    Visual and Circulatory Biophysics Laboratory, Department of Biomedical Engineering, Boston University, Massachusetts 02215, USA
    Biophys J 84:623-33. 2003
    ..Experiments that demonstrate and evaluate the microchannel measuring capabilities are presented and potential improvements and extensions are discussed...
  39. ncbi request reprint Continuous separation of biomolecules by the laterally asymmetric diffusion array with out-of-plane sample injection
    Mario Cabodi
    School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA
    Electrophoresis 23:3496-503. 2002
    ..We expect this type of device could now be employed for separation of a variety of different biomolecules, ranging from long dsDNA to small proteins...
  40. pmc Enhanced Caenorhabditis elegans locomotion in a structured microfluidic environment
    Sungsu Park
    Division of Nano Sciences BK21, Ewha Womans University, Seoul, Korea
    PLoS ONE 3:e2550. 2008
    ..In order to investigate how worms move in such environments, we have developed a technique to study C. elegans locomotion in microstructures fabricated from agar...