Keith Garlid

Summary

Affiliation: Portland State University
Country: USA

Publications

  1. pmc The mitochondrial K(ATP) channel--fact or fiction?
    Keith D Garlid
    Department of Biology, Portland State University, Portland, OR 97201 0751, USA
    J Mol Cell Cardiol 52:578-83. 2012
  2. pmc Cardioprotective signaling to mitochondria
    Keith D Garlid
    Department of Biology, Portland State University, Portland, OR 97201 0751, USA
    J Mol Cell Cardiol 46:858-66. 2009
  3. ncbi request reprint Mitochondrial potassium transport: the role of the mitochondrial ATP-sensitive K(+) channel in cardiac function and cardioprotection
    Keith D Garlid
    Department of Biology, Portland State University, 1719 SW 10th Avenue, PO Box 751, Portland, OR 97207, USA
    Biochim Biophys Acta 1606:1-21. 2003
  4. ncbi request reprint Mitochondrial potassium transport: the K(+) cycle
    Keith D Garlid
    Department of Biology, Portland State University, 1719 SW 10th Avenue, PO Box 751, Portland, OR 97207, USA
    Biochim Biophys Acta 1606:23-41. 2003
  5. pmc Intramitochondrial signaling: interactions among mitoKATP, PKCepsilon, ROS, and MPT
    Alexandre D T Costa
    Dept of Biology, Portland State Univ, PO Box 751, Portland, OR 97201 0751, USA
    Am J Physiol Heart Circ Physiol 295:H874-82. 2008
  6. ncbi request reprint cGMP signalling in pre- and post-conditioning: the role of mitochondria
    Alexandre D T Costa
    Department of Biology, Portland State University, PO Box 751, Portland, OR 97201, USA
    Cardiovasc Res 77:344-52. 2008
  7. ncbi request reprint The mechanism by which the mitochondrial ATP-sensitive K+ channel opening and H2O2 inhibit the mitochondrial permeability transition
    Alexandre D T Costa
    Department of Biology, Portland State University, Portland, Oregon 97201 0751, USA
    J Biol Chem 281:20801-8. 2006
  8. ncbi request reprint The direct physiological effects of mitoK(ATP) opening on heart mitochondria
    Alexandre D T Costa
    Dept of Biology, Portland State Univ, PO Box 751, Portland, OR 97207, USA
    Am J Physiol Heart Circ Physiol 290:H406-15. 2006
  9. ncbi request reprint Reconstitution of recombinant uncoupling proteins: UCP1, -2, and -3 have similar affinities for ATP and are unaffected by coenzyme Q10
    Martin Jaburek
    Department of Biology, Portland State University, Portland, Oregon 97207, USA
    J Biol Chem 278:25825-31. 2003
  10. ncbi request reprint Mitochondrial PKC epsilon and mitochondrial ATP-sensitive K+ channel copurify and coreconstitute to form a functioning signaling module in proteoliposomes
    Martin Jaburek
    Department of Biology, Portland State University, PO Box 751, Portland, Oregon 97207, USA
    Circ Res 99:878-83. 2006

Collaborators

Detail Information

Publications26

  1. pmc The mitochondrial K(ATP) channel--fact or fiction?
    Keith D Garlid
    Department of Biology, Portland State University, Portland, OR 97201 0751, USA
    J Mol Cell Cardiol 52:578-83. 2012
    ..is the evidence for the presence of a K(ATP) channel in mitochondria? Are the pharmacological agents used to modulate mitoK(ATP) activity sufficiently specific to allow the role of these channels in cardioprotection to be established?..
  2. pmc Cardioprotective signaling to mitochondria
    Keith D Garlid
    Department of Biology, Portland State University, Portland, OR 97201 0751, USA
    J Mol Cell Cardiol 46:858-66. 2009
    ..We review the experimental findings that bear on these hypotheses and other modes of protection involving mitochondria...
  3. ncbi request reprint Mitochondrial potassium transport: the role of the mitochondrial ATP-sensitive K(+) channel in cardiac function and cardioprotection
    Keith D Garlid
    Department of Biology, Portland State University, 1719 SW 10th Avenue, PO Box 751, Portland, OR 97207, USA
    Biochim Biophys Acta 1606:1-21. 2003
    ..MitoK(ATP)-mediated volume regulation, in turn, prevents disruption of the structure-function of the IMS and facilitates efficient energy transfers between mitochondria and myofibrillar ATPases...
  4. ncbi request reprint Mitochondrial potassium transport: the K(+) cycle
    Keith D Garlid
    Department of Biology, Portland State University, 1719 SW 10th Avenue, PO Box 751, Portland, OR 97207, USA
    Biochim Biophys Acta 1606:23-41. 2003
    ..This review discusses the properties of the mitochondrial K(+) cycle that help to understand the basis of these diverse effects...
  5. pmc Intramitochondrial signaling: interactions among mitoKATP, PKCepsilon, ROS, and MPT
    Alexandre D T Costa
    Dept of Biology, Portland State Univ, PO Box 751, Portland, OR 97201 0751, USA
    Am J Physiol Heart Circ Physiol 295:H874-82. 2008
    ..This feedback pathway may be responsible for the lasting protective effect of preconditioning, colloquially known as the memory effect...
  6. ncbi request reprint cGMP signalling in pre- and post-conditioning: the role of mitochondria
    Alexandre D T Costa
    Department of Biology, Portland State University, PO Box 751, Portland, OR 97201, USA
    Cardiovasc Res 77:344-52. 2008
    ..The resulting ROS then activate a second PKC pool which, through another signal transduction pathway termed the mediator pathway, causes inhibition of MPT and reduction in cell death...
  7. ncbi request reprint The mechanism by which the mitochondrial ATP-sensitive K+ channel opening and H2O2 inhibit the mitochondrial permeability transition
    Alexandre D T Costa
    Department of Biology, Portland State University, Portland, Oregon 97201 0751, USA
    J Biol Chem 281:20801-8. 2006
    ..4 (+/-0.1) microm. On the basis of these results, we propose that two different PKCepsilon pools regulate this signaling pathway, one in association with mitoK(ATP) and the other in association with MPT...
  8. ncbi request reprint The direct physiological effects of mitoK(ATP) opening on heart mitochondria
    Alexandre D T Costa
    Dept of Biology, Portland State Univ, PO Box 751, Portland, OR 97207, USA
    Am J Physiol Heart Circ Physiol 290:H406-15. 2006
    ..These results provide decisive support for the hypothesis that mitochondria contain an ATP-sensitive K+ channel and establish the physiological consequences of mitoK(ATP) opening for mitochondria...
  9. ncbi request reprint Reconstitution of recombinant uncoupling proteins: UCP1, -2, and -3 have similar affinities for ATP and are unaffected by coenzyme Q10
    Martin Jaburek
    Department of Biology, Portland State University, Portland, Oregon 97207, USA
    J Biol Chem 278:25825-31. 2003
    ..We conclude that coenzyme Q is not a cofactor of UCP-mediated proton transport...
  10. ncbi request reprint Mitochondrial PKC epsilon and mitochondrial ATP-sensitive K+ channel copurify and coreconstitute to form a functioning signaling module in proteoliposomes
    Martin Jaburek
    Department of Biology, Portland State University, PO Box 751, Portland, Oregon 97207, USA
    Circ Res 99:878-83. 2006
    ..In addition, the activating effect of PKC agonists was reversed by exogenous protein phosphatase 2A. These results demonstrate persistent, functional association of mitochondrial PKC epsilon and mitoK(ATP)...
  11. ncbi request reprint Opening mitoKATP increases superoxide generation from complex I of the electron transport chain
    Anastasia Andrukhiv
    Dept of Biology, Portland State Univ, PO Box 751, Portland, OR 97207, USA
    Am J Physiol Heart Circ Physiol 291:H2067-74. 2006
    ..Myxothiazol stimulated mitoK(ATP)-dependent ROS production, whereas rotenone had no effect. This indicates that the superoxide originates in complex I (NADH:ubiquinone oxidoreductase) of the electron transport chain...
  12. pmc Conditioning the heart induces formation of signalosomes that interact with mitochondria to open mitoKATP channels
    Casey L Quinlan
    Department of Biology, Portland State University, PO Box 751, Portland, OR 97201 0751, USA
    Am J Physiol Heart Circ Physiol 295:H953-H961. 2008
    ..These results provide initial support for a novel mechanism for signal transmission from a plasma membrane receptor to mitoKATP channels...
  13. ncbi request reprint Inhibition of cardiac contractility by 5-hydroxydecanoate and tetraphenylphosphonium ion: a possible role of mitoKATP in response to inotropic stress
    Keith D Garlid
    Portland State University, Portland, Oregon, USA
    Am J Physiol Heart Circ Physiol 291:H152-60. 2006
    ..These results indicate a physiological role for mitoKATP in inotropy and, by extension, in heart failure...
  14. ncbi request reprint Protein kinase G transmits the cardioprotective signal from cytosol to mitochondria
    Alexandre D T Costa
    Department of Biology, Portland State University, Portland, Ore, USA
    Circ Res 97:329-36. 2005
    ..We conclude PKG is the terminal cytosolic component of the trigger pathway; it transmits the cardioprotective signal from cytosol to inner mitochondrial membrane by a pathway that includes PKC-epsilon...
  15. ncbi request reprint Functional distinctions between the mitochondrial ATP-dependent K+ channel (mitoKATP) and its inward rectifier subunit (mitoKIR)
    Galina D Mironova
    Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia
    J Biol Chem 279:32562-8. 2004
    ..Tetraphenylphosphonium inhibits K(+) flux through both mitoKIR and mitoK(ATP) with the same apparent affinity. These findings support the hypothesis that the 55-kDa mitoKIR is the channel component of mitoK(ATP)...
  16. ncbi request reprint Ouabain protects rat hearts against ischemia-reperfusion injury via pathway involving src kinase, mitoKATP, and ROS
    Philippe Pasdois
    Institut National de la Santé et de la Recherche, Médicale U441, University Victor Segalen Bordeaux 2, University Hospital of Bordeaux, Bordeaux, France
    Am J Physiol Heart Circ Physiol 292:H1470-8. 2007
    ..However, only PP2, 5-HD, and MPG blocked ouabain-induced cardioprotection...
  17. ncbi request reprint Mechanisms by which opening the mitochondrial ATP- sensitive K(+) channel protects the ischemic heart
    Pierre Dos Santos
    Unité 441 Athérosclérose and IFR 4, Institut National de la Sante et de la Recherche Medicale, 33600 Pessac, France
    Am J Physiol Heart Circ Physiol 283:H284-95. 2002
    ..These effects are proposed to lead to preservation of adenine nucleotides during ischemia and efficient energy transfer upon reperfusion...
  18. ncbi request reprint Opening of ATP-sensitive potassium channels causes generation of free radicals in vascular smooth muscle cells
    Maike Krenz
    Department of Physiology, MSB 3024, University of South Alabama, College of Medicine, Mobile, AL 36688, USA
    Basic Res Cardiol 97:365-73. 2002
    ..Furthermore, a potassium-selective ionophore can mimic the effect of putative mitochondrial KATP channel openers. We conclude that potassium movement through KATP directly leads to ROS production by the mitochondria...
  19. ncbi request reprint Involvement of mitogen-activated protein kinases and reactive oxygen species in the inotropic action of ouabain on cardiac myocytes. A potential role for mitochondrial K(ATP) channels
    Jiang Tian
    Department of Pharmacology, Medical College of Ohio, Toledo, OH 43614 5804, USA
    Mol Cell Biochem 242:181-7. 2003
    ..While activation of MAPKs leads to increases in [Ca2+]i, opening of mitochondrial K(ATP) channel relays the ouabain signal to increased ROS production in cardiac myocytes...
  20. doi request reprint Differential increase of mitochondrial matrix volume by sevoflurane in isolated cardiac mitochondria
    Matthias L Riess
    Department of Anesthesiology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Anesth Analg 106:1049-55, table of contents. 2008
    ..We investigated whether sevoflurane increases MMV and if this increase is mediated by mK(ATP) channel opening...
  21. ncbi request reprint Interactions of K+ATP channel blockers with Na+/K+-ATPase
    Lijun Liu
    Department of Physiology, Pharmacology, Metabolism, and Cardiovascular Sciences, The University of Toledo College of Medicine, Mail Stop 1008, Health Science Campus, 3000 Arlington Avenue, Toledo, OH 43614 2598, USA
    Mol Cell Biochem 306:231-7. 2007
    ....
  22. ncbi request reprint Sarcoplasmic ATP-sensitive potassium channel blocker HMR1098 protects the ischemic heart: implication of calcium, complex I, reactive oxygen species and mitochondrial ATP-sensitive potassium channel
    Philippe Pasdois
    INSERM U 828, France
    J Mol Cell Cardiol 42:631-42. 2007
    ..Taken together, these data suggest that a functional interaction between sarcK(ATP) and mitoK(ATP) exists in isolated rat heart ischemia model, which is mediated by extracellular calcium influx...
  23. pmc Ouabain triggers preconditioning through activation of the Na+,K+-ATPase signaling cascade in rat hearts
    Sandrine V Pierre
    Department of Physiology, Pharmacology, Metabolism and Cardiovascular Sciences, Medical University of Ohio, 3035 Arlington Avenue, Toledo, OH 43614 5804, USA
    Cardiovasc Res 73:488-96. 2007
    ....
  24. ncbi request reprint P1075 opens mitochondrial K(ATP) channels and generates reactive oxygen species resulting in cardioprotection of rabbit hearts
    Olaf Oldenburg
    Department of Physiology, University of South Alabama, College of Medicine, Mobile, AL 36688, USA
    J Mol Cell Cardiol 35:1035-42. 2003
    ..7 +/- 9.5% and 27.7 +/- 4.6% infarction, respectively; P = n.s. vs. untreated hearts). These data provide strong evidence that P1075 does open mitoK(ATP) channels and protects the ischemic rabbit heart in a mitoK(ATP)-dependent manner...
  25. ncbi request reprint Bepridil, an antiarrhythmic drug, opens mitochondrial KATP channels, blocks sarcolemmal KATP channels, and confers cardioprotection
    Toshiaki Sato
    Department of Pharmacology, Chiba University Graduate School of Medicine, 1 8 1 Inohana, Chuo Ku, Chiba 260 8670, Japan
    J Pharmacol Exp Ther 316:182-8. 2006
    ..This is the first report of a unique modulator of K(ATP) channels; bepridil would be expected to mitigate ischemic injury while blunting arrhythmias...
  26. ncbi request reprint Hydroperoxy fatty acid cycling mediated by mitochondrial uncoupling protein UCP2
    Martin Jaburek
    Department of Membrane Transport Biophysics, No 75, Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4, Czech Republic
    J Biol Chem 279:53097-102. 2004
    ....

Research Grants22

  1. FUNCTION AND REGULATION OF UNCOUPLING PROTEINS 2 AND 3
    Keith Garlid; Fiscal Year: 2002
    ..The significance of this project lies in determining the biochemical function and regulation of UCP2 and UCP3, which are unknown. ..
  2. Mitochondrial ATP-Sensitive K+ Channel in Heart
    Keith Garlid; Fiscal Year: 2005
    ..abstract_text> ..
  3. FUNCTION AND REGULATION OF UNCOUPLING PROTEINS 2 AND 3
    Keith Garlid; Fiscal Year: 2006
    ..These studies will provide new understanding of the mechanisms of UCP2 and 3 function and regulation and will also resolve mechanistic questions that have been raised about these proteins. ..
  4. The Mitochondrial ATP-Sensitive K+ Channel in Heart
    Keith Garlid; Fiscal Year: 2007
    ..Identification of signaling intermediates will utilize 32P-labeling and functional assays of phosphorylation. ..
  5. STRUCTURE/FUNCTION OF THE MITOCHONDRIAL K-ATP CHANNEL
    Keith Garlid; Fiscal Year: 2001
    ..K+ flux will also be measured in intact mitochondria in order to examine the physiological role of mitoKATP using control analysis. ..
  6. The Mitochondrial ATP-Sensitive K+ Channel in Heart
    Keith D Garlid; Fiscal Year: 2010
    ..Identification of signaling intermediates will utilize 32P-labeling and functional assays of phosphorylation. ..