Wei Wang

Summary

Affiliation: Pfizer Global Research and Development
Country: USA

Publications

  1. ncbi Peroxide formation in polysorbate 80 and protein stability
    Emily Ha
    Analytics and Formulation Department, Process Sciences, Bayer Biotechnology, 800 Dwight Way, Berkeley, California 94701, USA
    J Pharm Sci 91:2252-64. 2002
  2. ncbi Antibody structure, instability, and formulation
    Wei Wang
    Pfizer, Inc, Global Biologics, 700 Chesterfield Parkway West, Chesterfield, Missouri 63017, USA
    J Pharm Sci 96:1-26. 2007
  3. ncbi Dual effects of Tween 80 on protein stability
    Wei Wang
    Pfizer Inc, Global Biologics, 700 Chesterfield Parkway West, Chesterfield, MO 63017, USA
    Int J Pharm 347:31-8. 2008
  4. pmc Non-Arrhenius protein aggregation
    Wei Wang
    Pfizer Inc, BioTherapeutics Pharmaceutical Sciences, 700 Chesterfield Parkway West, Chesterfield, MO 63017, USA
    AAPS J 15:840-51. 2013
  5. doi Immunogenicity of protein aggregates--concerns and realities
    Wei Wang
    Pfizer BioTherapeutics Pharmaceutical Sciences, Chesterfield, MO 63017, USA
    Int J Pharm 431:1-11. 2012
  6. doi Protein aggregation--pathways and influencing factors
    Wei Wang
    Pfizer Inc, Global Biologics, 700 Chesterfield Parkway West, Chesterfield, MO 63017, United States
    Int J Pharm 390:89-99. 2010
  7. ncbi Protein aggregation and its inhibition in biopharmaceutics
    Wei Wang
    Biotechnology Division, Bayer HealthCare, 800 Dwight Way, Berkeley, CA 94701, USA
    Int J Pharm 289:1-30. 2005
  8. ncbi pH dependent effect of glycosylation on protein stability
    Wei Wang
    Pfizer Inc, Global Biologics, 700 Chesterfield Parkway West, Chesterfield, MO 63017, USA
    Eur J Pharm Sci 33:120-7. 2008
  9. doi Metal-leachate-induced conjugate protein instability
    Ning Li
    Pharmaceutical Research and Development, BioTherapeutics Pharmaceutical Sciences, Pfizer Inc, St Louis, Missouri, USA
    J Pharm Sci 101:2733-43. 2012
  10. ncbi Oxidation of protein by vaporized sanitizing agents
    Wei Wang
    Analytics and Formulation, Biotechnology Division, Bayer HealthCare, Berkeley, CA 94701, USA
    PDA J Pharm Sci Technol 58:121-9. 2004

Detail Information

Publications74

  1. ncbi Peroxide formation in polysorbate 80 and protein stability
    Emily Ha
    Analytics and Formulation Department, Process Sciences, Bayer Biotechnology, 800 Dwight Way, Berkeley, California 94701, USA
    J Pharm Sci 91:2252-64. 2002
    ....
  2. ncbi Antibody structure, instability, and formulation
    Wei Wang
    Pfizer, Inc, Global Biologics, 700 Chesterfield Parkway West, Chesterfield, Missouri 63017, USA
    J Pharm Sci 96:1-26. 2007
    ..Various aspects of formulation development have been examined to identify the critical attributes for the stabilization of antibodies...
  3. ncbi Dual effects of Tween 80 on protein stability
    Wei Wang
    Pfizer Inc, Global Biologics, 700 Chesterfield Parkway West, Chesterfield, MO 63017, USA
    Int J Pharm 347:31-8. 2008
    ..Addition of 0.1% Tween 80 not only increased the rate of oxidation in general but also altered the temperature-dependency of IL-2 mutein oxidation...
  4. pmc Non-Arrhenius protein aggregation
    Wei Wang
    Pfizer Inc, BioTherapeutics Pharmaceutical Sciences, 700 Chesterfield Parkway West, Chesterfield, MO 63017, USA
    AAPS J 15:840-51. 2013
    ....
  5. doi Immunogenicity of protein aggregates--concerns and realities
    Wei Wang
    Pfizer BioTherapeutics Pharmaceutical Sciences, Chesterfield, MO 63017, USA
    Int J Pharm 431:1-11. 2012
    ....
  6. doi Protein aggregation--pathways and influencing factors
    Wei Wang
    Pfizer Inc, Global Biologics, 700 Chesterfield Parkway West, Chesterfield, MO 63017, United States
    Int J Pharm 390:89-99. 2010
    ..This article is intended to review and discuss the latest understandings in protein aggregation pathways and the possible extrinsic factors that affect or control the protein aggregation process...
  7. ncbi Protein aggregation and its inhibition in biopharmaceutics
    Wei Wang
    Biotechnology Division, Bayer HealthCare, 800 Dwight Way, Berkeley, CA 94701, USA
    Int J Pharm 289:1-30. 2005
    ....
  8. ncbi pH dependent effect of glycosylation on protein stability
    Wei Wang
    Pfizer Inc, Global Biologics, 700 Chesterfield Parkway West, Chesterfield, MO 63017, USA
    Eur J Pharm Sci 33:120-7. 2008
    ..0 and both forms are most stable at pH 5.5 in terms of thermal stability, precipitation rate and total degradation rate. This study clearly demonstrates that the effect of glycosylation on the stability of a protein is pH-dependent...
  9. doi Metal-leachate-induced conjugate protein instability
    Ning Li
    Pharmaceutical Research and Development, BioTherapeutics Pharmaceutical Sciences, Pfizer Inc, St Louis, Missouri, USA
    J Pharm Sci 101:2733-43. 2012
    ..Such complexation may lead to formation of multimers that may exceed the solubility limit...
  10. ncbi Oxidation of protein by vaporized sanitizing agents
    Wei Wang
    Analytics and Formulation, Biotechnology Division, Bayer HealthCare, Berkeley, CA 94701, USA
    PDA J Pharm Sci Technol 58:121-9. 2004
    ..These results strongly suggest that the sanitization process needs to be properly controlled and closely monitored during manufacturing of drug products that are sensitive to oxidation...
  11. ncbi Coagulation factor VIII: structure and stability
    Wei Wang
    Analytics and Formulation, Biotechnology Division, Bayer HealthCare, 800 Dwight Way, Berkeley, CA 94701, USA
    Int J Pharm 259:1-15. 2003
    ....
  12. doi Quantitation of free polyethylene glycol in PEGylated protein conjugate by size exclusion HPLC with refractive index (RI) detection
    Ning Li
    Department of Pharmaceutical Research and Development, Pfizer Global Biologics, St Louis Laboratory, Pfizer Inc, St Louis, MO 63017, USA
    J Pharm Biomed Anal 48:1332-8. 2008
    ..These deviations resulted in no significant impact on area percent distribution of all species. However, separation was found to be sensitive to high ionic strength and buffer species...
  13. ncbi Stabilization of a polypeptide in non-aqueous solvents
    Wei Wang
    Bayer HealthCare, 800 Dwight Way, Berkeley, CA 94701, United States
    Int J Pharm 351:1-7. 2008
    ..The stabilization approaches are applicable only in a non-aqueous environment for this peptide, and possibly for other peptides with similar reactive moieties...
  14. doi Factors affecting cleavage at aspartic residues in model decapeptides
    Ning Li
    Pharmaceutical Research and Development, Global Biologics, Pfizer Inc, St Louis, MO 63017, USA
    J Pharm Biomed Anal 50:73-8. 2009
    ..A strong pH dependence of cleavage was observed for all decapeptides, especially when pH was <5. Three out of four decapeptides showed a clear Arrhenius temperature dependency whereas Asp-Pro-containing peptide did not...
  15. ncbi The discovery and preparation of disubstituted novel amino-aryl-piperidine-based renin inhibitors
    Wayne L Cody
    Department of Chemistry, Pfizer Global Research and Development, Michigan Laboratories, 2800 Plymouth Road, Ann Arbor, MI 48105, USA
    Bioorg Med Chem 13:59-68. 2005
    ....
  16. ncbi Synthesis of a spirocyclic indoline lactone
    John C Hodges
    Pfizer Global Research and Development, 2800 Plymouth Road, Ann Arbor, Michigan 48105, USA
    J Org Chem 69:2504-8. 2004
    ..N-Acylation, olefin dihydroxylation, and tert-butyl ester cleavage affords the spirocyclic lactone 18 as a pair of diastereomers. Isolation and characterization of individual diastereomers 18a and 18b are described...
  17. ncbi Correlation of rFVIII inactivation with aggregation in solution
    Wei Wang
    Analytics and Formulation, Process Sciences, Biotechnology, Bayer Corp, 800 Dwight Way, Berkeley, California 94701, USA
    Pharm Res 20:693-700. 2003
    ..This study was designed to investigate the stability of recombinant FVIII (rFVIII) in solution at different pHs and to probe the cause(s) of rFVIII inactivation under accelerated storage conditions...
  18. ncbi Agitation-induced aggregation and subvisible particulate formation in model proteins
    Murali Jayaraman
    Pharmaceutical Research and Development, Pfizer Inc, Chesterfield, MO, USA
    Eur J Pharm Biopharm 87:299-309. 2014
    ..Taken together, our data suggests that agitation-induced SbVP formation can occur through different mechanisms and can vary, depending on the protein and solution conditions. ..
  19. ncbi Engineering novel VPAC2-selective agonists with improved stability and glucose-lowering activity in vivo
    Clark Q Pan
    Bayer HealthCare, Berkelye, California, USA
    J Pharmacol Exp Ther 320:900-6. 2007
    ..These PEGylated peptides retain the ability to selectively bind and activate the VPAC2 receptor and have prolonged glucose-lowering activity in vivo...
  20. pmc A rice high-affinity potassium transporter (HKT) conceals a calcium-permeable cation channel
    Wen Zhi Lan
    Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 107:7089-94. 2010
    ..The work also raises questions about the evolutionary changes in this protein family following the divergence of monocots and dicots...
  21. ncbi Human secretory signal peptide description by hidden Markov model and generation of a strong artificial signal peptide for secreted protein expression
    Steve Barash
    Department of Information Technology, Human Genome Sciences, Inc, Rockville, MD 20850, USA
    Biochem Biophys Res Commun 294:835-42. 2002
    ..The applications of high-bit scoring HMM signals in recombinant protein production and protein engineering are discussed...
  22. ncbi A novel method for removing residual acetone from gelatin microspheres
    Wei Wang
    Biotechnology, Bayer Corporation, Berkeley, CA 94701, USA
    Pharm Dev Technol 7:169-80. 2002
    ..To develop a method for removing residual acetone from gelatin microspheres...
  23. ncbi Delayed secretory pathway contributions to VLDL-triglycerides from plasma NEFA, diet, and de novo lipogenesis in humans
    Aruna Vedala
    Department of Nutritional Sciences and Toxicology, University of California at Berkeley, Berkeley, CA 94720, USA
    J Lipid Res 47:2562-74. 2006
    ..The turnover time and sources of this pool differ in diabetic HPTG and nondiabetic HPTG, with potential therapeutic implications...
  24. ncbi Organocatalytic enantioselective cascade Michael-alkylation reactions: synthesis of chiral cyclopropanes and investigation of unexpected organocatalyzed stereoselective ring opening of cyclopropanes
    Hexin Xie
    Department of Chemistry and Chemical Biology, University of New Mexico, Albuquerque, New Mexico 87131 0001, USA
    J Am Chem Soc 129:10886-94. 2007
    ..This investigation represents the first example of an organocatalyst-promoted ring opening of the cyclopropanes, whereas such reactions have been intensively explored by Lewis acid-based catalysis...
  25. pmc Effects of disulfide bonds on folding behavior and mechanism of the beta-sheet protein tendamistat
    Meng Qin
    National Laboratory of Solid State Microstructure and Department of Physics, Nanjing University, Nanjing 210093, China
    Biophys J 90:272-86. 2006
    ..The implementation of this work indicates that the modified Gō-like model really describes the folding behavior of protein tendamistat and could be used to study the folding of other disulfide-bonded proteins...
  26. ncbi Characterization of peptide-pyrazole interactions in solution by low-temperature NMR studies
    Wei Wang
    Freie Universitat Berlin, Institut fur Organische Chemie, Takustrasse 3, 14195 Berlin, Germany
    Chemistry 13:854-61. 2007
    ..Based on amide chemical shift changes, the relative strength of intermolecular hydrogen bonds can be assessed and correlated with the electronic effects of the substituents on the pyrazole...
  27. ncbi HMGA1a protein unfolds or refolds synthetic DNA-chromophore hybrid polymers: a chaperone-like behavior
    Wei Wan
    Department of Chemistry, Washington State University, Pullman, WA 99164, USA
    Chembiochem 9:304-11. 2008
    ..The unfolding mechanisms and the binding stoichiometry of protein-hybrid polymers depended on the sequence of the synthetic polymers...
  28. doi Identifying folding nucleus based on residue contact networks of proteins
    Jie Li
    National Laboratory of Solid State Microstructure, Department of Physics, Nanjing University, Nanjing 210093, China
    Proteins 71:1899-907. 2008
    ..Our study shows that the FNRs can be identified solely from the native structures of proteins based on the analysis of residue contact network without any knowledge of the transition state ensemble...
  29. doi Discovery of azetidinone acids as conformationally-constrained dual PPARalpha/gamma agonists
    Wei Wang
    Metabolic Diseases Chemistry, Bristol Myers Squibb Research and Development, Princeton, NJ 08543 5400, USA
    Bioorg Med Chem Lett 18:1939-44. 2008
    ..Synthesis, in vitro and in vivo activities of compounds in this series are described. A high-yielding method for N-arylation of azetidinone esters is also described...
  30. doi All-atom replica exchange molecular simulation of protein BBL
    Jian Zhang
    Department of Physics, National Laboratory of Solid State Microstructure, Institute of Biophysics, Nanjing University, Nanjing, China
    Proteins 72:1038-47. 2008
    ..Compared with the experimental data (Sadqi et al., Nature 2006; 442:317), our results indicate that the Naf-BBL (pH5.3) may have an even lower barrier height and cooperativity...
  31. doi Molecular self-assembly inhibition leads to basket-shaped cyclophane formation with chiral dynamics
    Wei Wang
    Department of Chemistry, Washington State University, Pullman, Washington 99164, USA
    J Phys Chem A 112:3094-103. 2008
    ..An activation enthalpy barrier of 13.4 +/- 0.5 kcal.mol-1 for twisting the perylene plane in order to convert from one enantiomer to the other has been found...
  32. doi Crystal structure of long-chain alkane monooxygenase (LadA) in complex with coenzyme FMN: unveiling the long-chain alkane hydroxylase
    Liu Li
    Tsinghua Nankai IBP Joint Research Group for Structural Biology, Tsinghua University, Beijing 100084, China
    J Mol Biol 376:453-65. 2008
    ..The LadA structure opens a rational perspective to explore and alter the substrate binding site of LadA, with potential biotechnological applications in areas such as petroleum exploration and treatment of environmental oil pollution...
  33. ncbi Cyclization and catenation directed by molecular self-assembly
    Wei Wang
    Department of Chemistry, Washington State University, Pullman, Washington 99164, USA
    J Am Chem Soc 128:11150-9. 2006
    ....
  34. ncbi The use of LC/MS, GC/MS, and LC/NMR hyphenated techniques to identify a drug degradation product in pharmaceutical development
    Changkang Pan
    Pharmaceutical and Analytical Development, Novartis Pharmaceuticals Corporation, One Health Plaza, East Hanover, NJ 07936, USA
    J Pharm Biomed Anal 40:581-90. 2006
    ..The combination approach is critical for unambiguous impurity structure elucidation in drug degradation studies of pharmaceutical drug products...
  35. ncbi Bioactive compounds from the seeds of Punica granatum (pomegranate)
    Ru Feng Wang
    Laboratory of Pharmaceutical Sciences, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, People s Republic of China
    J Nat Prod 67:2096-8. 2004
    ..Compounds 1-4 exhibited antioxidant activity, which was evaluated by measurement of low-density lipoprotein (LDL) susceptibility to oxidation and by determination in vitro of malondialdehyde (MDA) levels in the rat brain...
  36. ncbi Thermodynamic stability and kinetic foldability of a lattice protein model
    Jie Li
    National Lab of Solid State Microstructure and Physics Department, Nanjing University, Nanjing 210093, China
    J Chem Phys 120:6274-87. 2004
    ..The folding of model proteins with the MMJ potentials is fast. However, the relationship between the foldability and the thermodynamic stability of the mutated sequences is complex...
  37. ncbi Stereoselective synthesis of dipeptide beta-turn mimetics: 7-benzyl and 8-phenyl substituted azabicyclo[4.3.0]nonane amino acid esters
    Wei Wang
    Department of Chemistry, University of Arizona, Tucson, Arizona 85721, USA
    J Org Chem 67:6353-60. 2002
    ..The resulting amino acids 8 and 14 were converted to the beta-turn mimetics 6,5-bicyclic lactams 1a-d in high yields...
  38. ncbi Folding behaviors of lattice model proteins with three kinds of contact potentials
    Meng Qin
    National Laboratory of Solid State Microstructure and Department of Physics, Nanjing University, China
    Phys Rev E Stat Nonlin Soft Matter Phys 67:061905. 2003
    ..The formations of various contacts are also analyzed, which provide us with some microscopic information on the model systems and interaction potentials...
  39. ncbi Kinetic transition in model proteins with a denatured native spinodal
    Jun Wang
    National Laboratory of Solid State Microstructure and Department of Physics, Nanjing University, Nanjing 210093, China
    Phys Rev E Stat Nonlin Soft Matter Phys 65:041925. 2002
    ..The connection of T(k) with the foldability is also studied by a Z-score-like quantity T(f)/T(k)...
  40. ncbi Exploiting sequence and structure homologs to identify protein-protein binding sites
    Jo Lan Chung
    Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla 92093 0537, USA
    Proteins 62:630-40. 2006
    ..The results support the hypothesis that in many cases protein interfaces require some residues to provide rigidity to minimize the entropic cost upon complex formation...
  41. ncbi Folding with downhill behavior and low cooperativity of proteins
    Guanghong Zuo
    Department of Physics, Nanjing University, China
    Proteins 63:165-73. 2006
    ..These could enable us to identify the downhill folding or the cooperative two-state folding behavior solely from the native structures of proteins...
  42. ncbi A general asymmetric synthesis of syn- and anti-beta-substituted cysteine and serine derivatives
    Chiyi Xiong
    Department of Chemistry, University of Arizona, Tucson, Arizona 85721, USA
    J Org Chem 67:3514-7. 2002
    ..The high regio- and stereoselective ring-opening reactions of cyclic sulfates and aziridines provided enantiomerically pure beta-substituted cysteine and serine derivatives...
  43. ncbi Regioselective and stereoselective nucleophilic ring opening reactions of a phenyl-substituted aziridine: enantioselective synthesis of beta-substituted tryptophan, cysteine, and serine derivatives
    Chiyi Xiong
    Department of Chemistry, University of Arizona, Tucson, Arizona 85721, USA
    J Org Chem 67:1399-402. 2002
    ..This general approach can be used to synthesize a variety of beta-substituted novel amino acids...
  44. ncbi Novel fiber coated with amide bridged-calix[4]arene used for solid-phase microextraction of aliphatic amines
    Xiujuan Li
    Department of Chemistry, Wuhan University, Wuhan 430072, China
    J Chromatogr A 1041:1-9. 2004
    ..9% (n = 5). The results of fish freshness assay indicate the present method is a validated and simple procedure for the simultaneous determination of TMA in fish...
  45. ncbi CG base pair recognition by substituted phenylimidazole nucleosides
    Wei Wang
    Institut fur Chemie, Freie Universitat Berlin, Germany
    Org Biomol Chem 2:1194-8. 2004
    ..2D NOE measurements at low temperatures confirm the proposed binding mode for the high-affinity ligands but indicate binding interactions for the weakly bound analogues different from the expected geometry...
  46. doi Proline-catalyzed direct inverse electron demand diels-alder reactions of ketones with 1,2,4,5-tetrazines
    Hexin Xie
    Department of Chemistry and Chemical Biology, University of New Mexico, Albuquerque, New Mexico 87131 0001, USA
    Org Lett 10:1923-6. 2008
    ..An organocatalytic direct inverse electron demand Diels-Alder reaction of ketones with 1,2,4,5-tetrazines has been developed. The process is efficiently catalyzed by proline to give Diels-Alder adducts pyridazines in high yields...
  47. pmc Entropic contributions and the influence of the hydrophobic environment in promiscuous protein-protein association
    Chia en A Chang
    Departments of Chemistry and Biochemistry and Pharmacology, Center for Theoretical Biological Physics Howard Hughes Medical Institute, University of California at San Diego, La Jolla, CA 92093 0365, USA
    Proc Natl Acad Sci U S A 105:7456-61. 2008
    ..Implications are discussed for understanding and treating diseases in which promiscuous protein interactions are used...
  48. pmc Crystallization and preliminary crystallographic analysis of human Ca 2+-loaded calbindin-D28k
    Chang Zhang
    Tsinghua Nankai IBP Joint Research Group for Structural Biology, Tsinghua University, Beijing 100084, People s Republic of China
    Acta Crystallogr Sect F Struct Biol Cryst Commun 64:133-6. 2008
    ..1, b = 28.2, c = 70.6 A, beta = 107.8 degrees . The presence of one molecule per asymmetric unit is presumed, corresponding to a Matthews coefficient of 1.75 A(3) Da(-1)...
  49. ncbi The number of protein folds and their distribution over families in nature
    Xinsheng Liu
    National Lab of Solid State Microstructure, Department of Physics and Institute of Biophysics, Nanjing University, Nanjing, China
    Proteins 54:491-9. 2004
    ..In addition, the method of directly "enlarging" the sample to the population may be useful in inferring distributions of species in different fields...
  50. doi Drastically decreased reactivity of thiols and disulfides complexed by cucurbit[6]uril
    Lidia Strimbu Berbeci
    Center for Supramolecular Science and Department of Chemistry, University of Miami, Coral Gables, Florida 33124 0431, USA
    Org Lett 10:3721-4. 2008
    ..The CB6-complexed thiols show drastically decreased reactivity with several oxidants, while the CB6-bound disulfide also exhibits hindered reactivity with reducing agents, such as dithiothreitol...
  51. ncbi Computational design and characterization of a monomeric helical dinuclear metalloprotein
    Jennifer R Calhoun
    Department of Biochemistry and Molecular Biophysics, Johnson Foundation, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Mol Biol 334:1101-15. 2003
    ..DFsc is then a realization of complete de novo design, where backbone structure, activity, and sequence are specified in the design process...
  52. ncbi Folding versus self-assembling
    Alexander D Q Li
    Department of Chemistry and Center for Materials Research, Washington State University, Pullman, WA 99164, USA
    Chemistry 9:4594-601. 2003
    ..As a result, phenomena associated with folding or self-assembly of molecular species are observed when these conditions are met in solution...
  53. doi Chiral amine-catalyzed enantioselective cascade aza-ene-type cyclization reactions
    Liansuo Zu
    Department of Chemistry and Chemical Biology, University of New Mexico, Albuquerque, NM 87131 0001, USA
    Chemistry 14:6333-5. 2008
  54. ncbi Retrocyclin, an antiretroviral theta-defensin, is a lectin
    Wei Wang
    Department of Medicine, University of California, Los Angeles, CA 90095, USA
    J Immunol 170:4708-16. 2003
    ..Retrocyclin's ability to recognize and bind carbohydrate-containing surface molecules is integrally related to its ability to protect cells from HIV-1 infection...
  55. ncbi Determination of physiological thiols by electrochemical detection with piazselenole and its application in rat breast cancer cells 4T-1
    Wei Wang
    Key Laboratory of Eco Chemical Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China
    J Am Chem Soc 130:10846-7. 2008
    ..0952 + 0.4287 x CGSH (10(-8) M) and the detection limit (3sigma) as 83 pM. The proposed method was satisfactorily applied to the extracts of rat breast cancer cells 4T-1 for intracellular thiols detection...
  56. ncbi Investigations of photolysis and rebinding kinetics in myoglobin using proximal ligand replacements
    Wenxiang Cao
    Department of Physics and Center for Interdisciplinary Research on Complex Systems, Northeastern University, Boston, Massachusetts 02115, USA
    Biochemistry 43:11109-17. 2004
    ....
  57. ncbi Molecular and biochemical characterization of the Skp2-Cks1 binding interface
    Wei Wang
    Department of Chemistry and Biochemistry, University of Colorado Boulder, Boulder, Colorado 80309, USA
    J Biol Chem 279:51362-9. 2004
    ..These results uncovered the specificity of the Skp2-Cks1 interaction and reveal a critical function for the structurally flexible carboxyl-terminal tail region of Skp2 in Cks1 recognition and substrate ubiquitination...
  58. ncbi Enantioselective organocatalytic tandem Michael-Aldol reactions: one-pot synthesis of chiral thiochromenes
    Wei Wang
    Department of Chemistry, University of New Mexico, Albuquerque, New Mexico 87131 0001, USA
    J Am Chem Soc 128:10354-5. 2006
    ..The method affords one-pot access to chiral and synthetically useful thiochromenes in high yields and high enantioselectivities from readily available compounds...
  59. pmc Structure-based function inference using protein family-specific fingerprints
    Deepak Bandyopadhyay
    Department of Computer Science, University of North Carolina at Chapel Hill, North Carolina 27599, USA
    Protein Sci 15:1537-43. 2006
    ..Some predictions have been corroborated by other computational methods and some validated by subsequent functional characterization...
  60. ncbi Chiral binaphthyl-derived amine-thiourea organocatalyst-promoted asymmetric Morita-Baylis-Hillman reaction
    Jian Wang
    Department of Chemistry, University of New Mexico, Albuquerque, New Mexico 87131 0001, USA
    Org Lett 7:4293-6. 2005
    ..The process, catalyzed by the amine thiourea, affords synthetically valuable chiral allylic alcohol building blocks in high yields and high enantioselectivities...
  61. ncbi Synthesis of highly functionalized chiral cyclopentanes by catalytic enantio- and diastereoselective double Michael addition reactions
    Liansuo Zu
    Department of Chemistry, University of New Mexico, MSC03 2060, Albuquerque, NM 87131 0001, USA
    Angew Chem Int Ed Engl 46:3732-4. 2007
  62. ncbi Structural determination of seven new triterpenoids from Kadsura heteroclita by NMR techniques
    Wei Wang
    Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, P R China
    Magn Reson Chem 45:522-6. 2007
    ..Seven new triterpenoids, namely heteroclitalactones G-M (1-7), were isolated from the ethanol extract of the stems of Kadsura heteroclita. Structures of these compounds were characterized by extensive 1D and 2D NMR spectroscopic analyses...
  63. ncbi Grouping of amino acids and recognition of protein structurally conserved regions by reduced alphabets of amino acids
    Jing Li
    National Laboratory of Solid State Microstructure and Department of Physics, Nanjing University, Nanjing, 210093, China
    Sci China C Life Sci 50:392-402. 2007
    ..The results indicate that the accuracy or efficiency of sequence alignment can be improved with the optimal reduced alphabet with N around 9...
  64. ncbi [Human peripheral blood CD4+ T-lymphocytes reconstituting severe combined immunodeficient mouse-human cell immune system and its immunological function]
    Qiu Hua Zeng
    Department of Radiology, Third Xiangya Hospital, Central South University, Chaengsha 410013, China
    Zhong Nan Da Xue Xue Bao Yi Xue Ban 29:513-6. 2004
    ....
  65. ncbi Comparing graph representations of protein structure for mining family-specific residue-based packing motifs
    Jun Huan
    Department of Computer Science, University of North Carolina, 3239 Sitterson Hall, Chapel Hill, NC 27599, USA
    J Comput Biol 12:657-71. 2005
    ....
  66. ncbi Effects of solution conditions, processing parameters, and container materials on aggregation of a monoclonal antibody during freeze-thawing
    Lisa A Kueltzo
    Center for Pharmaceutical Biotechnology, Department of Pharmaceutical Sciences, University of Colorado Health Sciences Center, 4200 East 9th Avenue, Denver, Colorado 80262, USA
    J Pharm Sci 97:1801-12. 2008
    ..Samples stressed in plastic or glass containers contained low amounts of aggregate. Storage in Teflon or commercial freezing containers, however, led to significantly higher levels of aggregate formation...
  67. pmc On the detection of functionally coherent groups of protein domains with an extension to protein annotation
    William A McLaughlin
    Department of Chemistry and Biochemistry, Center for Theoretical Biological Physics, University of California, San Diego, 9500 Gilman Drive La Jolla, CA 92093 0359, USA
    BMC Bioinformatics 8:390. 2007
    ..Further effort is needed to identify groups of domains that span across two or more proteins and are linked by a cooperative function. Such functional domain combinations can be useful for protein annotation...
  68. ncbi Learning the drug target-likeness of a protein
    Huan Xu
    College of Life Science, Zhejiang University, Zijinhua Road, Hangzhou, Zhejiang, P R China
    Proteomics 7:4255-63. 2007
    ....
  69. ncbi Prokaryotic expression, refolding, and purification of fragment 450-650 of the spike protein of SARS-coronavirus
    Jin Cun Zhao
    Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China
    Protein Expr Purif 39:169-74. 2005
    ..In Western blot assays, the recombinant S450-650 was recognized by monoclonal Ab against the His-tag and also sera from a convalescent SARS patient. S450-650-based ELISA system was able to detect anti-SARS-CoV IgG Abs in patient sera...
  70. ncbi Preparation and identification of monoclonal antibodies against recombinant human thioredoxins
    Wen Xin Tang
    College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
    Hybridoma (Larchmt) 26:338-41. 2007
    ..We further analyzed their isotypes, titer, and affinity and found that those MAbs belong to the G1 subclass with kappa light chains, respectively. The MAbs were capable of recognizing hTrx-1, as determined by Western blotting...
  71. ncbi [An interventional study on amnestic mild cognitive impairment with small dose donepezil]
    Lu Ning Wang
    Department of Neurology, PLA General Hospital, Beijing 100853, China
    Zhonghua Nei Ke Za Zhi 43:760-3. 2004
    ....
  72. ncbi Organocatalytic enantioselective cascade Michael-aldol condensation reactions: efficient assembly of densely functionalized chiral cyclopentenes
    Jian Wang
    Department of Chemistry and Chemical Biology, University of New Mexico, MSC03 2060, Albuquerque, NM 87131 0001, USA
    Angew Chem Int Ed Engl 46:9050-3. 2007
  73. doi Metal-coupled folding of Cys2His2 zinc-finger
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    ....
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    ....