Brian J Stockman

Summary

Affiliation: Pfizer Global Research and Development
Country: USA

Publications

  1. ncbi Identification of allosteric PIF-pocket ligands for PDK1 using NMR-based fragment screening and 1H-15N TROSY experiments
    Brian J Stockman
    Pfizer Global Research and Development, Groton, CT 06340, USA
    Chem Biol Drug Des 73:179-88. 2009
  2. ncbi 2-Fluoro-ATP as a versatile tool for 19F NMR-based activity screening
    Brian J Stockman
    Pfizer Global Research and Development, Groton, Connecticut 06340, USA
    J Am Chem Soc 130:5870-1. 2008
  3. ncbi A nuclear magnetic resonance-based functional assay for nicotinamide adenine dinucleotide synthetase
    Brian J Stockman
    Pfizer Global Research and Development, Groton, Connecticut 06340, USA
    J Biomol Screen 12:457-63. 2007
  4. ncbi Resonance assignments for stromelysin complexed with a beta-sulfonyl hydroxamate inhibitor
    Parag V Sahasrabudhe
    Exploratory Medicinal Sciences, Pfizer Global Research and Development, MS 8118A 2024, Eastern Point Road, Groton, CT 06340, USA
    Biomol NMR Assign 3:183-6. 2009
  5. ncbi Physical methods to determine the binding mode of putative ligands for hepatitis C virus NS3 helicase
    Ronald W Sarver
    Structural, Analytical and Medicinal Chemistry, 301 Henrietta Street, Kalamazoo, MI 49001, USA
    Anal Biochem 309:186-95. 2002
  6. ncbi Multi-selective one dimensional proton NMR experiments for rapid screening and binding affinity measurements
    Claudio Dalvit
    Chemistry Department, Pharmacia, Viale Pasteur 10, 20014 Nerviano, MI, Italy
    Comb Chem High Throughput Screen 6:445-53. 2003
  7. ncbi Fluorine-NMR experiments for high-throughput screening: theoretical aspects, practical considerations, and range of applicability
    Claudio Dalvit
    Chemistry Department, Pharmacia, Viale Pasteur 10, 20014 Nerviano MI, Italy
    J Am Chem Soc 125:7696-703. 2003
  8. ncbi Competition binding experiments for rapidly ranking lead molecules for their binding affinity to human serum albumin
    Claudio Dalvit
    Chemistry Department, Pharmacia, Viale Pasteur 10, Nerviano MI, 20014, Italy
    Comb Chem High Throughput Screen 5:645-50. 2002
  9. ncbi Fluorine-NMR competition binding experiments for high-throughput screening of large compound mixtures
    Claudio Dalvit
    Chemistry Department, Pharmacia, Viale Pasteur 10, Nerviano MI, 20014, Italy
    Comb Chem High Throughput Screen 5:605-11. 2002
  10. ncbi High-throughput NMR-based screening with competition binding experiments
    Claudio Dalvit
    Chemistry Department, Pharmacia, Viale Pasteur 10, 20014 Nerviano MI, Milan, Italy
    J Am Chem Soc 124:7702-9. 2002

Collaborators

Detail Information

Publications11

  1. ncbi Identification of allosteric PIF-pocket ligands for PDK1 using NMR-based fragment screening and 1H-15N TROSY experiments
    Brian J Stockman
    Pfizer Global Research and Development, Groton, CT 06340, USA
    Chem Biol Drug Des 73:179-88. 2009
    ..Caliper assay data and 19F NMR assay data on the PDK1 Interacting Fragment pocket fragments and structurally related compounds identified them as potential allosteric activators of PDK1 function...
  2. ncbi 2-Fluoro-ATP as a versatile tool for 19F NMR-based activity screening
    Brian J Stockman
    Pfizer Global Research and Development, Groton, Connecticut 06340, USA
    J Am Chem Soc 130:5870-1. 2008
    ....
  3. ncbi A nuclear magnetic resonance-based functional assay for nicotinamide adenine dinucleotide synthetase
    Brian J Stockman
    Pfizer Global Research and Development, Groton, Connecticut 06340, USA
    J Biomol Screen 12:457-63. 2007
    ..The assay has been used to screen fragment libraries for NadE inhibitors...
  4. ncbi Resonance assignments for stromelysin complexed with a beta-sulfonyl hydroxamate inhibitor
    Parag V Sahasrabudhe
    Exploratory Medicinal Sciences, Pfizer Global Research and Development, MS 8118A 2024, Eastern Point Road, Groton, CT 06340, USA
    Biomol NMR Assign 3:183-6. 2009
    ..Almost 70% of assignable backbone and side-chain atoms were assigned in this highly dynamic protein...
  5. ncbi Physical methods to determine the binding mode of putative ligands for hepatitis C virus NS3 helicase
    Ronald W Sarver
    Structural, Analytical and Medicinal Chemistry, 301 Henrietta Street, Kalamazoo, MI 49001, USA
    Anal Biochem 309:186-95. 2002
    ..A combination of physical methods was required to discern the mode of action of compounds identified by HTS and remove undesirable lead templates from further consideration...
  6. ncbi Multi-selective one dimensional proton NMR experiments for rapid screening and binding affinity measurements
    Claudio Dalvit
    Chemistry Department, Pharmacia, Viale Pasteur 10, 20014 Nerviano, MI, Italy
    Comb Chem High Throughput Screen 6:445-53. 2003
    ..For a simple competition binding mechanism, the rapidly-derived NMR binding constants are in good agreement with the values derived from full-titration ITC and fluorescence spectroscopy measurements...
  7. ncbi Fluorine-NMR experiments for high-throughput screening: theoretical aspects, practical considerations, and range of applicability
    Claudio Dalvit
    Chemistry Department, Pharmacia, Viale Pasteur 10, 20014 Nerviano MI, Italy
    J Am Chem Soc 125:7696-703. 2003
    ..The experimental conditions together with the detection limits and binding constant measurements are investigated using human serum albumin as the target...
  8. ncbi Competition binding experiments for rapidly ranking lead molecules for their binding affinity to human serum albumin
    Claudio Dalvit
    Chemistry Department, Pharmacia, Viale Pasteur 10, Nerviano MI, 20014, Italy
    Comb Chem High Throughput Screen 5:645-50. 2002
    ..This review will summarize some of the NMR-based efforts towards developing universal, rapid, accurate, and site-specific assays for estimating protein binding...
  9. ncbi Fluorine-NMR competition binding experiments for high-throughput screening of large compound mixtures
    Claudio Dalvit
    Chemistry Department, Pharmacia, Viale Pasteur 10, Nerviano MI, 20014, Italy
    Comb Chem High Throughput Screen 5:605-11. 2002
    ..Finally, a novel approach for HTS using a reference molecule in combination with a control molecule is presented...
  10. ncbi High-throughput NMR-based screening with competition binding experiments
    Claudio Dalvit
    Chemistry Department, Pharmacia, Viale Pasteur 10, 20014 Nerviano MI, Milan, Italy
    J Am Chem Soc 124:7702-9. 2002
    ..With this approach it is possible to screen thousands of compounds in a short period of time against protein or DNA and RNA fragments. The methodology can also be applied for screening plant and fungi extracts...
  11. ncbi 1H nuclear magnetic resonance study of oxazolidinone binding to bacterial ribosomes
    Casey Chun Zhou
    Pharmacia Corporation, Kalamazoo, Michigan 49001, USA
    Antimicrob Agents Chemother 46:625-9. 2002
    ....