J L Workman

Summary

Affiliation: Pennsylvania State University
Country: USA

Publications

  1. ncbi Alteration of nucleosome structure as a mechanism of transcriptional regulation
    J L Workman
    Howard Hughes Medical Institute, Pennsylvania State University, University Park 16802, USA
    Annu Rev Biochem 67:545-79. 1998
  2. ncbi The ATM-related cofactor Tra1 is a component of the purified SAGA complex
    P A Grant
    Howard Hughes Medical Institute, Pennsylvania State University, University Park 16802 4500, USA
    Mol Cell 2:863-7. 1998
  3. ncbi Recruitment of HAT complexes by direct activator interactions with the ATM-related Tra1 subunit
    C E Brown
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, Department of Chemistry, The Pennsylvania State University, 306 Althouse Laboratory, University Park, PA 16802, USA
    Science 292:2333-7. 2001
  4. ncbi Transcriptional activators direct histone acetyltransferase complexes to nucleosomes
    R T Utley
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802 4500, USA
    Nature 394:498-502. 1998
  5. ncbi The ADA complex is a distinct histone acetyltransferase complex in Saccharomyces cerevisiae
    A Eberharter
    Howard Hughes Medical Institute and Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802 4500, USA
    Mol Cell Biol 19:6621-31. 1999
  6. ncbi Histone H3 specific acetyltransferases are essential for cell cycle progression
    L Howe
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Genes Dev 15:3144-54. 2001
  7. ncbi Stimulation of transcription factor binding and histone displacement by nucleosome assembly protein 1 and nucleoplasmin requires disruption of the histone octamer
    P P Walter
    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802, USA
    Mol Cell Biol 15:6178-87. 1995
  8. ncbi Activation domain-mediated targeting of the SWI/SNF complex to promoters stimulates transcription from nucleosome arrays
    K E Neely
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802, USA
    Mol Cell 4:649-55. 1999
  9. ncbi Remodeling chromatin structures for transcription: what happens to the histones?
    D J Steger
    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802 4500, USA
    Bioessays 18:875-84. 1996
  10. ncbi Persistent site-specific remodeling of a nucleosome array by transient action of the SWI/SNF complex
    T Owen Hughes
    Department of Biochemistry and Molecular Biology and Center for Gene Regulation, Pennsylvania State University, University Park, PA 16802 4500, USA
    Science 273:513-6. 1996

Collaborators

Detail Information

Publications75

  1. ncbi Alteration of nucleosome structure as a mechanism of transcriptional regulation
    J L Workman
    Howard Hughes Medical Institute, Pennsylvania State University, University Park 16802, USA
    Annu Rev Biochem 67:545-79. 1998
    ..The direct modification of nucleosome structure by these complexes is likely to play a central role in appropriate regulation of eukaryotic genes...
  2. ncbi The ATM-related cofactor Tra1 is a component of the purified SAGA complex
    P A Grant
    Howard Hughes Medical Institute, Pennsylvania State University, University Park 16802 4500, USA
    Mol Cell 2:863-7. 1998
    ..These results indicate a role for Tra1 in the regulation of transcriptional activation through the recruitment of HAT activity to an activator-bound promoter...
  3. ncbi Recruitment of HAT complexes by direct activator interactions with the ATM-related Tra1 subunit
    C E Brown
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, Department of Chemistry, The Pennsylvania State University, 306 Althouse Laboratory, University Park, PA 16802, USA
    Science 292:2333-7. 2001
    ..These data demonstrate that the essential Tra1 protein serves as a common target for activators in both SAGA and NuA4 acetyltransferases...
  4. ncbi Transcriptional activators direct histone acetyltransferase complexes to nucleosomes
    R T Utley
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802 4500, USA
    Nature 394:498-502. 1998
    ..Our results demonstrate the targeting of native HAT complexes by a transcription-activation domain to nucleosomes in order to activate transcription...
  5. ncbi The ADA complex is a distinct histone acetyltransferase complex in Saccharomyces cerevisiae
    A Eberharter
    Howard Hughes Medical Institute and Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802 4500, USA
    Mol Cell Biol 19:6621-31. 1999
    ..These results indicate that Gcn5, Ada2, and Ada3 function as part of a unique HAT complex (ADA) and represent shared subunits between this complex and SAGA...
  6. ncbi Histone H3 specific acetyltransferases are essential for cell cycle progression
    L Howe
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Genes Dev 15:3144-54. 2001
    ..The strikingly similar effect of loss of combined essential H3 HAT activities and the loss of a single essential H4 HAT underscores the fundamental biological significance of each of these chromatin-modifying activities...
  7. ncbi Stimulation of transcription factor binding and histone displacement by nucleosome assembly protein 1 and nucleoplasmin requires disruption of the histone octamer
    P P Walter
    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802, USA
    Mol Cell Biol 15:6178-87. 1995
    ..Thus, histone displacement in this instance occurred by transfer of complete histone octamers, a mechanism distinct from that mediated by the histone-binding proteins nucleoplasmin and NAP-1...
  8. ncbi Activation domain-mediated targeting of the SWI/SNF complex to promoters stimulates transcription from nucleosome arrays
    K E Neely
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802, USA
    Mol Cell 4:649-55. 1999
    ..The similarity of activation domain interactions and transcriptional stimulation between SWI/SNF and the SAGA histone acetyltransferase complex may account for their apparent overlapping functions in vivo...
  9. ncbi Remodeling chromatin structures for transcription: what happens to the histones?
    D J Steger
    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802 4500, USA
    Bioessays 18:875-84. 1996
    ....
  10. ncbi Persistent site-specific remodeling of a nucleosome array by transient action of the SWI/SNF complex
    T Owen Hughes
    Department of Biochemistry and Molecular Biology and Center for Gene Regulation, Pennsylvania State University, University Park, PA 16802 4500, USA
    Science 273:513-6. 1996
    ..Thus, the transient action of the SWI/SNF complex facilitated irreversible disruption of transcription factor-bound nucleosomes...
  11. ncbi Expanded lysine acetylation specificity of Gcn5 in native complexes
    P A Grant
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802 4500, USA
    J Biol Chem 274:5895-900. 1999
    ..Furthermore Ada and SAGA have overlapping, yet distinct, patterns of acetylation, suggesting that the association of specific subunits determines site specificity...
  12. ncbi A subset of TAF(II)s are integral components of the SAGA complex required for nucleosome acetylation and transcriptional stimulation
    P A Grant
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802 4500, USA
    Cell 94:45-53. 1998
    ..These results illustrate a role for certain TAF(II) proteins in the regulation of gene expression at the level of chromatin modification that is distinct from the TFIID complex and TAF(II)145...
  13. ncbi The many HATs of transcription coactivators
    C E Brown
    Howard Hughes Medical Institute, Dept of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802 4500, USA
    Trends Biochem Sci 25:15-9. 2000
    ..In addition to histones, some HATs can acetylate non-histone proteins suggesting multiple roles for these enzymes...
  14. ncbi Sds3 (suppressor of defective silencing 3) is an integral component of the yeast Sin3[middle dot]Rpd3 histone deacetylase complex and is required for histone deacetylase activity
    T Lechner
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802 4500, USA
    J Biol Chem 275:40961-6. 2000
    ..Moreover, the remaining Rpd3p complex in the sds3Delta strain had little or no histone deacetylase activity. Thus, Sds3p plays important roles in the integrity and catalytic activity of the Rpd3p.Sin3p complex...
  15. ncbi Binding of the wheat basic leucine zipper protein EmBP-1 to nucleosomal binding sites is modulated by nucleosome positioning
    X Niu
    Department of Horticulture, Pennsylvania State University, University Park 16802, USA
    Plant Cell 8:1569-87. 1996
    ..We conclude that positioning of recognition sequences on a nucleosome may play an important role in regulating interactions of EmBP-1 with its target sites in plant cells...
  16. ncbi Purification and biochemical heterogeneity of the mammalian SWI-SNF complex
    W Wang
    Howard Hughes Medical Institute, Department of Developmental Biology, Stanford University, CA 94305 5428, USA
    EMBO J 15:5370-82. 1996
    ..Certain cell lines completely lack BRG1 and hbrm, indicating that they are not essential for cell viability and that the mammalian SWI-SNF complex may be tailored to the needs of a differentiated cell type...
  17. ncbi The diverse functions of histone acetyltransferase complexes
    Michael J Carrozza
    Department of Biochemistry and Molecular Biology, Howard Hughes Medical Institute, Penn State University, University Park, PA 16803, USA
    Trends Genet 19:321-9. 2003
    ..The high conservation of the acetyltransferase complexes and their functions illustrates their central role in cell growth and development...
  18. ncbi MSL complex is attracted to genes marked by H3K36 trimethylation using a sequence-independent mechanism
    Erica Larschan
    Howard Hughes Medical Institute, Brigham and Women s Hospital, 75 Francis Street, Boston, MA 02115, USA
    Mol Cell 28:121-33. 2007
    ..Our results support a model in which MSL complex uses high-affinity sites to initially recognize the X chromosome and then associates with many of its targets through sequence-independent features of transcribed genes...
  19. ncbi The Set2 histone methyltransferase functions through the phosphorylated carboxyl-terminal domain of RNA polymerase II
    Bing Li
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802 4500, USA
    J Biol Chem 278:8897-903. 2003
    ..Collectively, these results suggest that Set2 is involved in regulating transcription elongation through its direct contact with pol II...
  20. ncbi Yng1p modulates the activity of Sas3p as a component of the yeast NuA3 Hhistone acetyltransferase complex
    LeAnn Howe
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802 4500, USA
    Mol Cell Biol 22:5047-53. 2002
    ....
  21. ncbi Function and selectivity of bromodomains in anchoring chromatin-modifying complexes to promoter nucleosomes
    Ahmed H Hassan
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, 306 Althouse Laboratory, University Park, PA 16802, USA
    Cell 111:369-79. 2002
    ..Thus, bromodomains within the catalytic subunits of SAGA and SWI/SNF anchor these complexes to acetylated promoter nucleosomes...
  22. ncbi Stimulation of GAL4 derivative binding to nucleosomal DNA by the yeast SWI/SNF complex
    J Cote
    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802
    Science 265:53-60. 1994
    ..A direct ATP-dependent interaction between the SWI/SNF complex and nucleosomal DNA was detected. These observations suggest that a primary role of the SWI/SNF complex is to promote activator binding to nucleosomal DNA...
  23. ncbi The DNA repair protein yKu80 regulates the function of recombination enhancer during yeast mating type switching
    Chun Ruan
    Huck Institutes of the Life Sciences, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Mol Cell Biol 25:8476-85. 2005
    ..We also detect directional movement of yKu80p from the RE towards HML during switching. These results indicate a novel function of yeast Ku80p in regulating mating type switching...
  24. ncbi Histone acetylation and chromatin remodeling: which comes first?
    Kristen E Neely
    Department of Biochemistry and Molecular Biology, Howard Hughes Medical Institute, The Pennsylvania State University, University Park 16802, USA
    Mol Genet Metab 76:1-5. 2002
  25. ncbi Gal80 confers specificity on HAT complex interactions with activators
    Michael J Carrozza
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    J Biol Chem 277:24648-52. 2002
    ..These studies demonstrate that the specificity of HAT/activator interactions can be dictated by cofactors that modulate activation domain function in response to cellular signals...
  26. ncbi Transcription activator interactions with multiple SWI/SNF subunits
    Kristen E Neely
    Department of Biochemistry and Molecular Biology, Howard Hughes Medical Institute, The Pennsylvania State University, University Park, PA 16802, USA
    Mol Cell Biol 22:1615-25. 2002
    ..Thus, three distinct subunits of the SWI/SNF complex contribute to its interactions with activation domains...
  27. ncbi The deubiquitylation activity of Ubp8 is dependent upon Sgf11 and its association with the SAGA complex
    Kenneth K Lee
    Stowers Institute for Medical Research, 1000 E 50th Street, Kansas City, MO 64110, USA
    Mol Cell Biol 25:1173-82. 2005
    ..Taken together, these data indicate that the expression of some genes, including ARG1, is regulated by a balance of histone H2B ubiquitylation in the cell...
  28. ncbi Characterization of the yeast trimeric-SAS acetyltransferase complex
    Wei Jong Shia
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 280:11987-94. 2005
    ..This specific lysine preference corresponds to the role of SAS complex in antagonizing the spreading of Sir proteins at silent loci in S. cerevisiae...
  29. ncbi In and out: histone variant exchange in chromatin
    Jingji Jin
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Trends Biochem Sci 30:680-7. 2005
    ..The incorporation of histone variants most probably has epigenetic consequences and contributes to architectural changes in chromosomes...
  30. ncbi The proteasome regulatory particle alters the SAGA coactivator to enhance its interactions with transcriptional activators
    Daeyoup Lee
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, Missouri 64110, USA
    Cell 123:423-36. 2005
    ..These results indicate that the 19S RP modulates SAGA complex using its ATPase components, thereby facilitating subsequent transcription events at promoters...
  31. ncbi Histone H3 methylation by Set2 directs deacetylation of coding regions by Rpd3S to suppress spurious intragenic transcription
    Michael J Carrozza
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Cell 123:581-92. 2005
    ..This erases transcription elongation-associated acetylation to suppress intragenic transcription initiation...
  32. ncbi Stable incorporation of sequence specific repressors Ash1 and Ume6 into the Rpd3L complex
    Michael J Carrozza
    Stowers Institute for Medical Research1000 East 50th Street, Kansas City, MO 64110, USA
    Biochim Biophys Acta 1731:77-87; discussion 75-6. 2005
    ..Thus, the Rpd3L complex is directly recruited to specific promoters through multiple integral DNA-binding proteins...
  33. ncbi Preferential occupancy of histone variant H2AZ at inactive promoters influences local histone modifications and chromatin remodeling
    Bing Li
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Proc Natl Acad Sci U S A 102:18385-90. 2005
    ..These lines of evidence collectively suggest that Htz1 may serve to mark quiescent promoters for proper activation...
  34. ncbi The heterochromatin protein 1 (HP1) family: put away a bias toward HP1
    So Hee Kwon
    Stowers Institute for Medical Research, 1000 East 50th, Kansas City, Missouri 64110, USA
    Mol Cells 26:217-27. 2008
    ..Therefore, HP1 proteins might participate in both transcription repression in heterochromatin and euchromatin...
  35. ncbi Histone ubiquitination: triggering gene activity
    Vikki M Weake
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Mol Cell 29:653-63. 2008
    ....
  36. ncbi SAS-mediated acetylation of histone H4 Lys 16 is required for H2A.Z incorporation at subtelomeric regions in Saccharomyces cerevisiae
    Wei Jong Shia
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Genes Dev 20:2507-12. 2006
    ..Z incorporation near telomeres. The presence of H4 Lys 16 acetylation and H2A.Z synergistically prevent the ectopic propagation of heterochromatin. Overall, our data suggest a novel antisilencing mechanism near telomeres...
  37. ncbi RSC exploits histone acetylation to abrogate the nucleosomal block to RNA polymerase II elongation
    Michael Carey
    Stowers Medical Research Institute, 1000 East 50th Street, Kansas City, Missouri 64110, USA
    Mol Cell 24:481-7. 2006
    ..Thus, RSC can recognize acetylated nucleosomes and facilitate passage of Pol II through them. These data support the view that histone modifications regulate accessibility of the coding region to Pol II...
  38. ncbi Infrequently transcribed long genes depend on the Set2/Rpd3S pathway for accurate transcription
    Bing Li
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Genes Dev 21:1422-30. 2007
    ..These data highlight the importance of using the functional readout of histone codes to define the roles of specific pathways...
  39. ncbi Acetylation by Tip60 is required for selective histone variant exchange at DNA lesions
    Thomas Kusch
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Science 306:2084-7. 2004
    ..Thus, these data reveal a previously unknown mechanism for selective histone exchange that uses the concerted action of two distinct chromatin-remodeling enzymes within the same multiprotein complex...
  40. ncbi Global position and recruitment of HATs and HDACs in the yeast genome
    Francois Robert
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA
    Mol Cell 16:199-209. 2004
    ....
  41. ncbi The novel SLIK histone acetyltransferase complex functions in the yeast retrograde response pathway
    Marilyn G Pray-Grant
    Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Mol Cell Biol 22:8774-86. 2002
    ....
  42. ncbi Sas4 and Sas5 are required for the histone acetyltransferase activity of Sas2 in the SAS complex
    Ann Sutton
    Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York 11794 5215, USA
    J Biol Chem 278:16887-92. 2003
    ....
  43. ncbi Opposite role of yeast ING family members in p53-dependent transcriptional activation
    Amine Nourani
    Laval University Cancer Research Center, Hôtel Dieu de Québec CHUQ, Quebec City, Quebec G1R 2J6, Canada
    J Biol Chem 278:19171-5. 2003
    ..They also demonstrate the key specific role of ING proteins in different chromatin modifying complexes and their opposite functions in p53-dependent transcription...
  44. ncbi In vitro targeting reveals intrinsic histone tail specificity of the Sin3/histone deacetylase and N-CoR/SMRT corepressor complexes
    Michiel Vermeulen
    Department of Molecular Biology, University of Nijmegen, 6500 HB Nijmegen, The Netherlands
    Mol Cell Biol 24:2364-72. 2004
    ..These results provide solid evidence that HDAC-containing complexes display distinct, intrinsic histone tail specificities and hence may function differently to regulate chromatin structure and transcription...
  45. ncbi Combined action of PHD and chromo domains directs the Rpd3S HDAC to transcribed chromatin
    Bing Li
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Science 316:1050-4. 2007
    ..Thus, the coupled chromo and PHD domains of Rpd3S specify recognition of the methyl H3K36 mark, demonstrating the first combinatorial domain requirement within a protein complex to read a specific histone code...
  46. ncbi RPAP1, a novel human RNA polymerase II-associated protein affinity purified with recombinant wild-type and mutated polymerase subunits
    Célia Jeronimo
    Laboratory of Gene Transcription, Institut de Recherches Cliniques de Montreal, Montreal, Quebec, Canada H2W 1R7
    Mol Cell Biol 24:7043-58. 2004
    ....
  47. ncbi Activation domains drive nucleosome eviction by SWI/SNF
    José L Gutiérrez
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    EMBO J 26:730-40. 2007
    ..We propose a model mechanism for this transcription factor-mediated enhancement of SWI/SNF octamer transfer activity...
  48. ncbi The role of chromatin during transcription
    Bing Li
    Stowers Medical Research Institute, 1000 East 50 th Street, Kansas City, MO 64110, USA
    Cell 128:707-19. 2007
    ..In this Review, we highlight advances in our understanding of chromatin regulation and discuss how such regulation affects the binding of transcription factors as well as the initiation and elongation steps of transcription...
  49. ncbi Histone acetyltransferase complexes: one size doesn't fit all
    Kenneth K Lee
    Stowers Institute, 1000 East 50th Street, Kansas City, Missouri 64110, USA
    Nat Rev Mol Cell Biol 8:284-95. 2007
    ....
  50. ncbi Histone variants and complexes involved in their exchange
    Thomas Kusch
    Department of Biochemistry and Molecular Biology, Room 123A, Rutgers University, 604 Allison Road, Piscataway, NJ 08854, USA
    Subcell Biochem 41:91-109. 2007
    ....
  51. ncbi Global nucleosome distribution and the regulation of transcription in yeast
    Sevinc Ercan
    Stowers Institute for Medical Research, 1, 000 East 50th Street, Kansas City, MO 64110, USA
    Genome Biol 5:243. 2004
    ..This may be the result of transcription factors displacing nucleosomes...
  52. ncbi The mammalian YL1 protein is a shared subunit of the TRRAP/TIP60 histone acetyltransferase and SRCAP complexes
    Yong Cai
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 280:13665-70. 2005
    ..Taken together, our findings identify the YL1 protein as a new subunit of the TRRAP/TIP60 HAT complex, and they suggest that YL1 plays multiple roles in chromatin modification and remodeling in cells...
  53. ncbi The complexity of chromatin remodeling and its links to cancer
    Kristen E Neely
    Howard Hughes Medical Institute and Department of Biochemistry and Molecular Biology, The Pennsylvania State University, 306 Althouse Laboratory, University Park, PA 16802, USA
    Biochim Biophys Acta 1603:19-29. 2002
    ..Their subunit composition and brief overview of their functional properties will be discussed. Also, current links between human chromatin-remodeling complexes and cell growth and cancer will be presented...
  54. ncbi SAGA-mediated H2B deubiquitination controls the development of neuronal connectivity in the Drosophila visual system
    Vikki M Weake
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    EMBO J 27:394-405. 2008
    ..Candidate genes regulated by SAGA that may be required for correct axon targeting were identified by microarray analysis of gene expression in SAGA mutants...
  55. ncbi Preparation of nuclear and cytoplasmic extracts from mammalian cells
    Susan M Abmayr
    The Stowers Institute for Medical Research, Kansas City, Missouri, USA
    Curr Protoc Mol Biol . 2006
    ..Additional protocols describe optimization of the method to increase the yield of specific proteins, adaptation of the method for downstream applications such as affinity purification, and preparation of the cytoplasmic (S-100) fraction...
  56. ncbi ATAC is a double histone acetyltransferase complex that stimulates nucleosome sliding
    Tamaki Suganuma
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, Missouri 64110, USA
    Nat Struct Mol Biol 15:364-72. 2008
    ..melanogaster embryos. Furthermore, although ATAC does not show nucleosome-remodeling activity itself, it stimulates nucleosome sliding by the ISWI, SWI-SNF and RSC complexes...
  57. ncbi Chromosome and expression mechanisms: a year dominated by histone modifications, transitory and remembered
    Sarah C R Elgin
    Curr Opin Genet Dev 12:127-9. 2002
  58. ncbi Diverse functions of WD40 repeat proteins in histone recognition
    Tamaki Suganuma
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Genes Dev 22:1265-8. 2008
    ..1313-1318) demonstrate that the WD40 repeat protein p55 binds a structured region of H4 through a novel binding pocket on the side of beta-propeller, illustrating a diversity of histone recognition by WD40 repeat proteins...
  59. ncbi Clearing the way for unpaused polymerases
    Vikki M Weake
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Cell 134:16-8. 2008
    ..Petesch and Lis (2008) now show that nucleosomes are lost across the entire Hsp70 locus in an initial wave that precedes transcription by RNA polymerase II...
  60. ncbi Mechanism of transcription factor recruitment by acidic activators
    Monica E Ferreira
    Department of Life Sciences, Södertörns högskola, S 141 89 Huddinge, Sweden
    J Biol Chem 280:21779-84. 2005
    ....
  61. ncbi SWI/SNF displaces SAGA-acetylated nucleosomes
    Mark Chandy
    Stowers Institute for Medical Research, 1000 E 50th St, Kansas City, MO 64110, USA
    Eukaryot Cell 5:1738-47. 2006
    ..These data indicate that targeted histone acetylation by the SAGA complex predisposes promoter nucleosomes for displacement by the SWI/SNF complex...
  62. ncbi Yeast recombination enhancer is stimulated by transcription activation
    Sevinc Ercan
    Stowers Institute for Medical Research, 1000 East 50th St, Kansas City, MO 64110, USA
    Mol Cell Biol 25:7976-87. 2005
    ..We conclude that the role of Mcm1 in RE is to open chromatin around the conserved domains and activate transcription; this facilitates Fkh1 binding and the level of this binding determines the level of donor preference...
  63. ncbi Two Drosophila Ada2 homologues function in different multiprotein complexes
    Thomas Kusch
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Mol Cell Biol 23:3305-19. 2003
    ..This suggests that the mammalian and fly homologues of the transcriptional adapter Ada2 form two functionally distinct subgroups with unique characteristics...
  64. ncbi A mammalian chromatin remodeling complex with similarities to the yeast INO80 complex
    Jingji Jin
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    J Biol Chem 280:41207-12. 2005
    ..Finally, we demonstrate that, similar to the yeast INO80 complex, the hINO80 complex exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding...
  65. ncbi Histones: should I stay or should I go?
    Bing Li
    Stowers Institute for Medical Research, 1, 000 East 50th Street, Kansas City, MO 64110, USA
    Genome Biol 6:306. 2005
    ..A report on the American Society for Biochemistry and Molecular Biology symposium 'Transcriptional Regulation by Chromatin and RNA polymerase II', Lake Tahoe, USA, 29 October-1 November 2004...
  66. ncbi The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF
    Philippe Prochasson
    The Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Genes Dev 19:2534-9. 2005
    ..Furthermore, HIR complex binding to nucleosomes forms a distinct protein/DNA complex resistant to remodeling by SWI/SNF. Thus, the HIR complex is a novel nucleosome assembly complex which functions with SWI/SNF to regulate transcription...
  67. ncbi Histone H3 variants and modifications on transcribed genes
    Jerry L Workman
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Proc Natl Acad Sci U S A 101:1429-30. 2004
  68. ncbi Chromatin dynamics rule the genome
    Samantha G Pattenden
    Stowers Institute for Medical Research, 1000 E 50th Street, Kansas City, MO 64110, USA
    Genome Biol 6:355. 2005
  69. ncbi Transcription factors prominently in Lasker Award to Roeder
    Susan M Abmayr
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Cell 115:243-6. 2003
  70. ncbi Repairing nucleosomes during transcription
    Michael J Carrozza
    Nat Struct Biol 10:879-80. 2003
  71. ncbi Breast cancer metastasis suppressor 1 (BRMS1) forms complexes with retinoblastoma-binding protein 1 (RBP1) and the mSin3 histone deacetylase complex and represses transcription
    William J Meehan
    Department of Pathology, Jake Gittlen Cancer Research Institute, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033 0850, USA
    J Biol Chem 279:1562-9. 2004
    ..These results further show that BRMS1 may participate in transcriptional regulation via interaction with the mSin3.HDAC complex and suggest a novel mechanism by which BRMS1 might suppress cancer metastasis...
  72. ncbi Histone H4 lysine 16 acetylation breaks the genome's silence
    Wei-Jong Shia
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Genome Biol 7:217. 2006
    ..A recent biochemical study pinpoints this particular acetylation mark as a switch for changing chromatin from a repressive to a transcriptionally active state...
  73. ncbi Targeting activity is required for SWI/SNF function in vivo and is accomplished through two partially redundant activator-interaction domains
    Philippe Prochasson
    Howard Hughes Medical Institute and Department of Biochemistry and Molecular Biology, 306 Althouse Laboratory, The Pennsylvania State University, University Park, PA 16802, USA
    Mol Cell 12:983-90. 2003
    ..Thus, two distinct activator-interaction domains play overlapping roles in the targeting activity of SWI/SNF, which is essential for its function in vivo...
  74. ncbi The essential gene wda encodes a WD40 repeat subunit of Drosophila SAGA required for histone H3 acetylation
    Sebastián Guelman
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Mol Cell Biol 26:7178-89. 2006
    ..Our results point to a critical function of dSAGA and histone acetylation during Drosophila development...
  75. ncbi Nucleosome displacement in transcription
    Jerry L Workman
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Genes Dev 20:2009-17. 2006
    ..It is becoming increasingly clear that the eukaryotic transcriptional machinery is adapted to exploit the presence of nucleosomes in very sophisticated ways...