DAVID PHELPS

Summary

Affiliation: Pennsylvania State University
Country: USA

Publications

  1. pmc Sex differences in the response of the alveolar macrophage proteome to treatment with exogenous surfactant protein-A
    David S Phelps
    Center for Host defense, Inflammation, and Lung Disease CHILD Research and Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA
    Proteome Sci 10:44. 2012
  2. pmc Sex differences in the impact of ozone on survival and alveolar macrophage function of mice after Klebsiella pneumoniae infection
    Anatoly N Mikerov
    The Penn State Center for Host defense, Inflammation, and Lung Disease CHILD Research, Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Respir Res 9:24. 2008
  3. pmc Ablation of SP-A has a negative impact on the susceptibility of mice to Klebsiella pneumoniae infection after ozone exposure: sex differences
    Anatoly N Mikerov
    The Penn State Center for Host defense, Inflammation, and Lung Disease Research, Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Respir Res 9:77. 2008
  4. pmc In vivo rescue of alveolar macrophages from SP-A knockout mice with exogenous SP-A nearly restores a wild type intracellular proteome; actin involvement
    David S Phelps
    Center for Host defense, Inflammation, and Lung Disease CHILD Research and Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Proteome Sci 9:67. 2011
  5. pmc Differences in the BAL proteome after Klebsiella pneumoniae infection in wild type and SP-A-/- mice
    Mehboob Ali
    Penn State Center for Host defense, Inflammation, and Lung Disease CHILD Research, Hershey, PA 17033, USA
    Proteome Sci 8:34. 2010
  6. pmc The impact of surfactant protein-A on ozone-induced changes in the mouse bronchoalveolar lavage proteome
    Rizwanul Haque
    Penn State Center for Host defense, Inflammation, and Lung Disease CHILD Research and the Department of Pediatrics, Penn State College of Medicine, Hershey, PA, USA
    Proteome Sci 7:12. 2009
  7. ncbi request reprint Surfactant regulation of host defense function in the lung: a question of balance
    D S Phelps
    Dep of Pediatrics, Room C7814, Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA
    Pediatr Pathol Mol Med 20:269-92. 2001
  8. ncbi request reprint Increased surfactant protein-A levels in patients with newly diagnosed idiopathic pulmonary fibrosis
    David S Phelps
    Department of Pediatrics, Penn State College of Medicine, PO Box 850, Hershey, PA 17033, USA
    Chest 125:617-25. 2004
  9. ncbi request reprint Surfactant protein A increases matrix metalloproteinase-9 production by THP-1 cells
    Luis G Vazquez de Lara
    Dept of Pediatrics, Pennsylvania State Univ College of Medicine, PO Box 850, Hershey, PA 17033, USA
    Am J Physiol Lung Cell Mol Physiol 285:L899-906. 2003
  10. pmc Surfactant protein A, an innate immune factor, is expressed in the vaginal mucosa and is present in vaginal lavage fluid
    Colin MacNeill
    Department of Obstetrics and Gynecology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Immunology 111:91-9. 2004

Research Grants

Collaborators

Detail Information

Publications28

  1. pmc Sex differences in the response of the alveolar macrophage proteome to treatment with exogenous surfactant protein-A
    David S Phelps
    Center for Host defense, Inflammation, and Lung Disease CHILD Research and Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA
    Proteome Sci 10:44. 2012
    ..abstract:..
  2. pmc Sex differences in the impact of ozone on survival and alveolar macrophage function of mice after Klebsiella pneumoniae infection
    Anatoly N Mikerov
    The Penn State Center for Host defense, Inflammation, and Lung Disease CHILD Research, Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Respir Res 9:24. 2008
    ..The purpose of this study was to determine whether ozone exposure differentially affects: 1) survival of male and female mice infected with Klebsiella pneumoniae, and 2) the phagocytic ability of macrophages from these mice...
  3. pmc Ablation of SP-A has a negative impact on the susceptibility of mice to Klebsiella pneumoniae infection after ozone exposure: sex differences
    Anatoly N Mikerov
    The Penn State Center for Host defense, Inflammation, and Lung Disease Research, Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Respir Res 9:77. 2008
    ..We hypothesized that ablation of SP-A has a negative impact on the susceptibility of mice to Klebsiella pneumoniae infection after ozone exposure, and that sex differences in the effect of ozone do exist...
  4. pmc In vivo rescue of alveolar macrophages from SP-A knockout mice with exogenous SP-A nearly restores a wild type intracellular proteome; actin involvement
    David S Phelps
    Center for Host defense, Inflammation, and Lung Disease CHILD Research and Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Proteome Sci 9:67. 2011
    ..abstract:..
  5. pmc Differences in the BAL proteome after Klebsiella pneumoniae infection in wild type and SP-A-/- mice
    Mehboob Ali
    Penn State Center for Host defense, Inflammation, and Lung Disease CHILD Research, Hershey, PA 17033, USA
    Proteome Sci 8:34. 2010
    ..Mice lacking SP-A are more susceptible to infection than wild type C57BL/6 mice. We studied bronchoalveolar lavage (BAL) protein expression in wild type and SP-A-/- mice infected with Klebsiella pneumoniae by 2D-DIGE...
  6. pmc The impact of surfactant protein-A on ozone-induced changes in the mouse bronchoalveolar lavage proteome
    Rizwanul Haque
    Penn State Center for Host defense, Inflammation, and Lung Disease CHILD Research and the Department of Pediatrics, Penn State College of Medicine, Hershey, PA, USA
    Proteome Sci 7:12. 2009
    ....
  7. ncbi request reprint Surfactant regulation of host defense function in the lung: a question of balance
    D S Phelps
    Dep of Pediatrics, Room C7814, Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA
    Pediatr Pathol Mol Med 20:269-92. 2001
    ..However, in some types of lung disease or after certain insults or exposures, the balance between these inhibitory and stimulatory influences may be disrupted and result in inflammatory injury...
  8. ncbi request reprint Increased surfactant protein-A levels in patients with newly diagnosed idiopathic pulmonary fibrosis
    David S Phelps
    Department of Pediatrics, Penn State College of Medicine, PO Box 850, Hershey, PA 17033, USA
    Chest 125:617-25. 2004
    ..To measure surfactant protein-A (SP-A) in the BAL of patients with idiopathic pulmonary fibrosis (IPF)...
  9. ncbi request reprint Surfactant protein A increases matrix metalloproteinase-9 production by THP-1 cells
    Luis G Vazquez de Lara
    Dept of Pediatrics, Pennsylvania State Univ College of Medicine, PO Box 850, Hershey, PA 17033, USA
    Am J Physiol Lung Cell Mol Physiol 285:L899-906. 2003
    ..We speculate that SP-A may influence the protease/antiprotease balance in the lungs of patients with quantitative and/or qualitative changes in surfactant constituents favoring an abnormal breakdown of extracellular matrix components...
  10. pmc Surfactant protein A, an innate immune factor, is expressed in the vaginal mucosa and is present in vaginal lavage fluid
    Colin MacNeill
    Department of Obstetrics and Gynecology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Immunology 111:91-9. 2004
    ..A corollary hypothesis is that qualitative and quantitative alterations of normal SP-A may play a role in the pathogenesis of lower genital tract inflammatory conditions...
  11. ncbi request reprint Altered chemokine response in an animal model of multiple organ dysfunction syndrome induced by zymosan
    Neal J Thomas
    Department of Pediatrics, Pediatric Critical Care Medicine, Penn State Children s Hospital, Penn State University College of Medicine, Hershey, PA 17033, USA
    J Pediatr Surg 40:464-9. 2005
    ..The aim of this study was to determine the monocyte chemoattractant protein-1 (MCP-1) response over time in an animal model of multiple organ dysfunction syndrome (MODS)...
  12. pmc The effect of ozone exposure on the ability of human surfactant protein a variants to stimulate cytokine production
    Guirong Wang
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Environ Health Perspect 110:79-84. 2002
    ....
  13. pmc Impact of ozone exposure on the phagocytic activity of human surfactant protein A (SP-A) and SP-A variants
    Anatoly N Mikerov
    Dept of Cellular and Molecular Physiology, The Pennsylvania State Univ College of Medicine, 500 University Dr Hershey, PA 17033, USA
    Am J Physiol Lung Cell Mol Physiol 294:L121-30. 2008
    ..We speculate that functional differences between SP-A1 and SP-A2 exist in vivo and that the redox status of the lung microenvironment differentially affects function of SP-A1 and SP-A2...
  14. pmc Role of surfactant protein-A (SP-A) in lung injury in response to acute ozone exposure of SP-A deficient mice
    Rizwanul Haque
    Department of Pediatrics, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Toxicol Appl Pharmacol 220:72-82. 2007
    ..In summary, several aspects of the inflammatory response differ between WT and KO mice. These in vivo findings appear to implicate SP-A in regulating inflammation and limiting epithelial damage in response to ozone exposure...
  15. ncbi request reprint Human SP-A genetic variants and bleomycin-induced cytokine production by THP-1 cells: effect of ozone-induced SP-A oxidation
    Weixiong Huang
    Departments of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Am J Physiol Lung Cell Mol Physiol 286:L546-53. 2004
    ..The results indicate that differences among SP-A variants may partly explain the individual variability of pulmonary complications observed during bleomycin chemotherapy and/or in an environment that may promote protein oxidation...
  16. ncbi request reprint Modulatory effects of ozone on THP-1 cells in response to SP-A stimulation
    Branislava Janic
    Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Am J Physiol Lung Cell Mol Physiol 288:L317-25. 2005
    ..These findings indicate that O(3) exposure may mediate its effect on macrophage function both directly and indirectly (via SP-A oxidation) and by involving different mechanisms...
  17. ncbi request reprint Differences in biochemical properties and in biological function between human SP-A1 and SP-A2 variants, and the impact of ozone-induced oxidation
    Guirong Wang
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Biochemistry 43:4227-39. 2004
    ..v) The SP-A2 variant (1A(0)) and the coexpressed protein 1A(0)/6A(2) inhibit ATP-stimulated PC secretion from alveolar type II cells to a greater extent than SP-A1 (6A(2)), a biologic activity that was susceptible to ozone treatment...
  18. pmc Surfactant protein A2 (SP-A2) variants expressed in CHO cells stimulate phagocytosis of Pseudomonas aeruginosa more than do SP-A1 variants
    Anatoly N Mikerov
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA
    Infect Immun 75:1403-12. 2007
    ..We conclude that SP-A2 variants stimulate phagocytosis of P. aeruginosa more effectively than SP-A1 variants and that posttranslational modifications positively influence the phagocytic activity of SP-A...
  19. ncbi request reprint Combined SP-A-bleomycin effect on cytokines by THP-1 cells: impact of surfactant lipids on this effect
    Weixiong Huang
    Department of Cellular and Molecular Physiology, Pennsylvania State College of Medicine, Hershey, Pennsylvania 17033, USA
    Am J Physiol Lung Cell Mol Physiol 283:L94-L102. 2002
    ....
  20. ncbi request reprint Factors affecting SP-A-mediated phagocytosis in human monocytic cell lines
    Jianqiang Ding
    Department of Cellular Physiology, Pennsylvania State College of Medicine, P O Box 850, Hershey, PA 17033, USA
    Respir Med 98:637-50. 2004
    ..Moreover, the stimulatory effect of SP-A was pH-dependent and appeared to be independent of several other phagocytic mechanisms, including those mediated by Fc receptors and mannose receptor...
  21. ncbi request reprint SP-A1 and SP-A2 variants differentially enhance association of Pseudomonas aeruginosa with rat alveolar macrophages
    Anatoly N Mikerov
    Dept of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Am J Physiol Lung Cell Mol Physiol 288:L150-8. 2005
    ..aeruginosa with rat alveolar macrophages differentially and in a concentration-dependent manner, with SP-A2 variants having a higher activity compared with SP-A1 variants...
  22. doi request reprint Age-related changes in the expression and oxidation of bronchoalveolar lavage proteins in the rat
    Todd M Umstead
    Penn State Center for Host defense, Inflammation, and Lung Disease CHILD Research, Department of Pediatrics, Penn State College of Medicine, PO Box 850, Hershey, PA 17033, USA
    Am J Physiol Lung Cell Mol Physiol 296:L14-29. 2009
    ..In addition, we have shown age-related increases in protein oxidation that may compromise host defense function in aged rats...
  23. pmc Down-regulation of 14-3-3 isoforms and annexin A5 proteins in lung adenocarcinoma induced by the tobacco-specific nitrosamine NNK in the A/J mouse revealed by proteomic analysis
    James D Bortner
    Department of Biochemistry and Molecular Biology, Penn State College of Medicine, Hershey, Pennsylvania 17033, USA
    J Proteome Res 8:4050-61. 2009
    ..Identification of these proteins in surrogate tissue in future studies would be highly useful in early detection of lung adenocarcinoma and clinical chemoprevention trials...
  24. doi request reprint Dual-platform proteomics study of plasma biomarkers in pediatric patients undergoing cardiopulmonary bypass
    Todd M Umstead
    Department of Pediatrics, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Pediatr Res 67:641-9. 2010
    ..The changes in levels of expression of these proteins may provide insight into the understanding, treatment, and prevention of systemic inflammation, thereby helping to improve the outcomes of pediatric CPB patients...
  25. ncbi request reprint Characterization of a human surfactant protein A1 (SP-A1) gene-specific antibody; SP-A1 content variation among individuals of varying age and pulmonary health
    Hephzibah Rani S Tagaram
    Departments of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Am J Physiol Lung Cell Mol Physiol 292:L1052-63. 2007
    ..10) compared with culture-positive NCF (0.368 +/- 0.085). In summary, we developed and characterized an SP-A1 gene-specific antibody and used it to identify gene-specific SP-A content in BALFs as a function of age and lung health...
  26. ncbi request reprint Surfactant protein A and B genetic variants predispose to idiopathic pulmonary fibrosis
    Moises Selman
    Instituto Nacional de Enfermedades Respiratorias, Mexico DF, Mexico
    Hum Genet 113:542-50. 2003
    ..These findings indicate that surfactant protein variants may serve as markers to identify subgroups of patients at risk, and we speculate that these contribute to IPF pathogenesis...
  27. ncbi request reprint An in vitro cell model system for the study of the effects of ozone and other gaseous agents on phagocytic cells
    Branislava Janic
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey 17033, USA
    J Immunol Methods 272:125-34. 2003
    ..This model system offers conditions where the experimental results are not due to cell death, but rather due to the effects of ozone and/or agents under investigation...
  28. ncbi request reprint Inhibition of ozone-induced SP-A oxidation by plant polyphenols
    Dimitrios Stagos
    Department of Biochemistry, University of Thessaly, Larissa, Greece
    Free Radic Res 41:357-66. 2007
    ..The results indicate that inhibition of SP-A oxidation by plant polyphenols may be a mechanism accounting for the protective activity of natural antioxidants against the effects of ozone exposure on lungs...

Research Grants5

  1. PULMONARY SURFACTANT EFFECTS ON THE MACROPHAGE PHENOTYPE
    DAVID PHELPS; Fiscal Year: 2000
    ..The object of these experiments is to confirm that the responses we are obtaining with the in vitro studies resemble the responses of actual immune cells. The volunteers are being used as a source of normal cells. ..
  2. PULMONARY SURFACTANT EFFECTS ON THE MACROPHAGE PHENOTYPE
    DAVID PHELPS; Fiscal Year: 2002
    ..The object of these experiments is to confirm that the responses we are obtaining with the in vitro studies resemble the responses of actual immune cells. The volunteers are being used as a source of normal cells. ..