W M Freeman

Summary

Affiliation: Pennsylvania State University
Country: USA

Publications

  1. pmc Proteomics for protein expression profiling in neuroscience
    Willard M Freeman
    Department of Pharmacology, Yerkes National Primate Research Center, Neuroscience Division, Emory University School of Medicine, Atlanta, Georgia 30329, USA
    Neurochem Res 29:1065-81. 2004
  2. pmc Future prospects for biomarkers of alcohol consumption and alcohol-induced disorders
    Willard M Freeman
    Department of Pharmacology, Penn State College of Medicine, Hershey, Pennsylvania 17033, USA
    Alcohol Clin Exp Res 34:946-54. 2010
  3. pmc The hippocampal neuroproteome with aging and cognitive decline: past progress and future directions
    Heather D VanGuilder
    Department of Pharmacology, Penn State College of Medicine, Milton S Hershey Medical Center Hershey, PA, USA
    Front Aging Neurosci 3:8. 2011
  4. pmc Age-related alterations in retinal neurovascular and inflammatory transcripts
    Colleen A Van Kirk
    Department of Pharmacology, Penn State College of Medicine, University Drive, Hershey, PA 17033, USA
    Mol Vis 17:1261-74. 2011
  5. pmc Multi-modal proteomic analysis of retinal protein expression alterations in a rat model of diabetic retinopathy
    Heather D VanGuilder
    Department of Pharmacology, Penn State College of Medicine, Hershey, Pennsylvania, United States of America
    PLoS ONE 6:e16271. 2011
  6. pmc Differences in the BAL proteome after Klebsiella pneumoniae infection in wild type and SP-A-/- mice
    Mehboob Ali
    Penn State Center for Host defense, Inflammation, and Lung Disease CHILD Research, Hershey, PA 17033, USA
    Proteome Sci 8:34. 2010
  7. pmc The impact of surfactant protein-A on ozone-induced changes in the mouse bronchoalveolar lavage proteome
    Rizwanul Haque
    Penn State Center for Host defense, Inflammation, and Lung Disease CHILD Research and the Department of Pediatrics, Penn State College of Medicine, Hershey, PA, USA
    Proteome Sci 7:12. 2009
  8. pmc Chronic insulin treatment of diabetes does not fully normalize alterations in the retinal transcriptome
    Georgina V Bixler
    Department of Pharmacology, Penn State College of Medicine, Hershey, PA, USA
    BMC Med Genomics 4:40. 2011
  9. pmc Persistent alterations in mesolimbic gene expression with abstinence from cocaine self-administration
    Willard M Freeman
    Department of Pharmacology, Penn State College of Medicine, Hershey, PA 17033, USA
    Neuropsychopharmacology 33:1807-17. 2008
  10. doi request reprint A multistep validation process of biomarkers for preclinical drug development
    W M Freeman
    Functional Genomics Core Facility, Penn State College of Medicine, Hershey, PA 17033, USA
    Pharmacogenomics J 10:385-95. 2010

Research Grants

  1. Gene expression and monoamine transporter reversal.
    Willard Freeman; Fiscal Year: 2002

Collaborators

Detail Information

Publications41

  1. pmc Proteomics for protein expression profiling in neuroscience
    Willard M Freeman
    Department of Pharmacology, Yerkes National Primate Research Center, Neuroscience Division, Emory University School of Medicine, Atlanta, Georgia 30329, USA
    Neurochem Res 29:1065-81. 2004
    ..Although there are many challenges in proteomic neuroscience, this field promises many rewards in the future...
  2. pmc Future prospects for biomarkers of alcohol consumption and alcohol-induced disorders
    Willard M Freeman
    Department of Pharmacology, Penn State College of Medicine, Hershey, Pennsylvania 17033, USA
    Alcohol Clin Exp Res 34:946-54. 2010
    ..We recommend that NIAAA consider taking a leadership role in organizing investigators in the field and providing a common set of clinical specimens for biomarker validation studies...
  3. pmc The hippocampal neuroproteome with aging and cognitive decline: past progress and future directions
    Heather D VanGuilder
    Department of Pharmacology, Penn State College of Medicine, Milton S Hershey Medical Center Hershey, PA, USA
    Front Aging Neurosci 3:8. 2011
    ..Progress in the field and challenges that remain to be addressed with regard to animal models, behavioral testing, and proteomic reporting are also discussed...
  4. pmc Age-related alterations in retinal neurovascular and inflammatory transcripts
    Colleen A Van Kirk
    Department of Pharmacology, Penn State College of Medicine, University Drive, Hershey, PA 17033, USA
    Mol Vis 17:1261-74. 2011
    ..The goal of this study was to investigate the effects of aging on retinal mRNA expression of neurovascular and inflammatory transcripts previously demonstrated to be regulated with diabetes...
  5. pmc Multi-modal proteomic analysis of retinal protein expression alterations in a rat model of diabetic retinopathy
    Heather D VanGuilder
    Department of Pharmacology, Penn State College of Medicine, Hershey, Pennsylvania, United States of America
    PLoS ONE 6:e16271. 2011
    ....
  6. pmc Differences in the BAL proteome after Klebsiella pneumoniae infection in wild type and SP-A-/- mice
    Mehboob Ali
    Penn State Center for Host defense, Inflammation, and Lung Disease CHILD Research, Hershey, PA 17033, USA
    Proteome Sci 8:34. 2010
    ..Mice lacking SP-A are more susceptible to infection than wild type C57BL/6 mice. We studied bronchoalveolar lavage (BAL) protein expression in wild type and SP-A-/- mice infected with Klebsiella pneumoniae by 2D-DIGE...
  7. pmc The impact of surfactant protein-A on ozone-induced changes in the mouse bronchoalveolar lavage proteome
    Rizwanul Haque
    Penn State Center for Host defense, Inflammation, and Lung Disease CHILD Research and the Department of Pediatrics, Penn State College of Medicine, Hershey, PA, USA
    Proteome Sci 7:12. 2009
    ....
  8. pmc Chronic insulin treatment of diabetes does not fully normalize alterations in the retinal transcriptome
    Georgina V Bixler
    Department of Pharmacology, Penn State College of Medicine, Hershey, PA, USA
    BMC Med Genomics 4:40. 2011
    ..The goal of this study was to identify molecular changes in the rodent retina induced by diabetes that are not normalized by insulin replacement and restoration of euglycemia...
  9. pmc Persistent alterations in mesolimbic gene expression with abstinence from cocaine self-administration
    Willard M Freeman
    Department of Pharmacology, Penn State College of Medicine, Hershey, PA 17033, USA
    Neuropsychopharmacology 33:1807-17. 2008
    ..This incubation is accompanied by changes in gene expression that persist long after cessation of drug administration and may be regulated by chromatin remodeling...
  10. doi request reprint A multistep validation process of biomarkers for preclinical drug development
    W M Freeman
    Functional Genomics Core Facility, Penn State College of Medicine, Hershey, PA 17033, USA
    Pharmacogenomics J 10:385-95. 2010
    ....
  11. ncbi request reprint Apo-AII is an elevated biomarker of chronic non-human primate ethanol self-administration
    Willard M Freeman
    Department of Pharmacology, H078, Penn State College of Medicine, 500 University Drive, PO Box 850, Hershey, PA 17033 0850, USA
    Alcohol Alcohol 41:300-5. 2006
    ....
  12. pmc Gene expression changes in the medial prefrontal cortex and nucleus accumbens following abstinence from cocaine self-administration
    Willard M Freeman
    Department of Pharmacology, Penn State College of Medicine, Hershey, PA 17033, USA
    BMC Neurosci 11:29. 2010
    ..Persistent changes in gene expression, as well as alterations induced during abstinence may underlie long-lasting drug craving and relapse liability...
  13. ncbi request reprint Depletion of abundant proteins from non-human primate serum for biomarker studies
    Willard M Freeman
    Department of Pharmacology H078, Penn State College of Medicine, 500 University Drive, Hershey, PA 17033, USA
    Proteomics 6:3109-13. 2006
    ..Depleted serum also demonstrated greater sensitivity for previously masked, lower-abundance proteins...
  14. pmc Concurrent hippocampal induction of MHC II pathway components and glial activation with advanced aging is not correlated with cognitive impairment
    Heather D VanGuilder
    Department of Pharmacology, Pennsylvania State University College of Medicine, 500 University Drive, Hershey, Pennsylvania 17057, USA
    J Neuroinflammation 8:138. 2011
    ..This study sought to comprehensively investigate expression of the MHC II immune response pathway and glial activation in the hippocampus in the context of both aging and age-related cognitive decline...
  15. ncbi request reprint Distinct proteomic profiles of amphetamine self-administration transitional states
    W M Freeman
    Department of Pharmacology, Penn State College of Medicine, Hershey, PA 17033, USA
    Pharmacogenomics J 5:203-14. 2005
    ..These data provide the first classification of addiction-related behaviors based on clustering of quantitative proteomic measurements. ...
  16. pmc Cognitive performance and age-related changes in the hippocampal proteome
    W M Freeman
    Department of Pharmacology, R130, Hershey Center for Applied Research, Penn State College of Medicine, 500 University Drive, Hershey, PA 17033, USA
    Neuroscience 159:183-95. 2009
    ....
  17. pmc Alterations in ionotropic glutamate receptor subunits during binge cocaine self-administration and withdrawal in rats
    Wenxue Tang
    Department of Pharmacology, Yerkes National Primate Research Center, Neuroscience Division, Emory University School of Medicine, Atlanta, Georgia 30329, USA
    J Neurochem 89:1021-33. 2004
    ..Because subunit composition determines the functional properties of iGluRs, the observed changes may indicate alterations in the excitability of dopamine transmission underlying long-term biochemical and behavioral effects of cocaine...
  18. pmc Gene expression changes following extinction testing in a heroin behavioral incubation model
    Kara L Kuntz-Melcavage
    Department of Pharmacology, Pennsylvania State University College of Medicine, Hershey, PA, USA
    BMC Neurosci 10:95. 2009
    ..Extinction sessions were conducted on groups after either 1 d or 14 d of drug-free enforced abstinence to demonstrate behavioral incubation...
  19. doi request reprint Effects of ischemic preconditioning and bevacizumab on apoptosis and vascular permeability following retinal ischemia-reperfusion injury
    STEVEN F ABCOUWER
    Department of Surgery, Penn State College of Medicine, Hershey, Milton S Hershey Medical Center, Hershey, PA 17033 0850, USA
    Invest Ophthalmol Vis Sci 51:5920-33. 2010
    ....
  20. doi request reprint Heroin self-administration: II. CNS gene expression following withdrawal and cue-induced drug-seeking behavior
    KARA L KUNTZ
    Department of Pharmacology, Pennsylvania State University College of Medicine, R130, 500 University Drive, Hershey, PA 17033, USA
    Pharmacol Biochem Behav 90:349-56. 2008
    ..These latter observations of persistent changes in gene expression following abstinence may reflect molecular correlates of relapse liability...
  21. pmc Gene expression profiles in HPV-immortalized human cervical cells treated with the nicotine-derived carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone
    Bogdan Prokopczyk
    Department of Pharmacology, Penn State College of Medicine, 500 University Drive, Hershey, PA 17033, USA
    Chem Biol Interact 177:173-80. 2009
    ..The results support previous epidemiological observations that, in addition to HPV, tobacco smoking also plays an important role in the development of cervical cancer...
  22. pmc Clinical application for the preservation of phospho-proteins through in-situ tissue stabilization
    C Bart Rountree
    Department of Pediatrics, Penn State Hershey Medical Center, 500 University Drive, Hershey, PA 17033
    Proteome Sci 8:61. 2010
    ..Challenges to the use of protein biomarkers include inter-sample variability introduced by differences in post-collection handling and ex vivo degradation of proteins and protein modifications...
  23. pmc Classification of alcohol abuse by plasma protein biomarkers
    Willard M Freeman
    Department of Pharmacology, Penn State College of Medicine, Hershey, Pennsylvania, USA
    Biol Psychiatry 68:219-22. 2010
    ..Previously, proposed single-analyte biochemical tests of alcohol intake suffer from low sensitivity and specificity or examine only acute drinking and have therefore seen limited clinical use...
  24. doi request reprint Correlating human and animal studies of cocaine abuse and gene expression
    Melinda E Lull
    Department of Pharmacology, Pennsylvania State College of Medicine, Hershey, PA 17033, USA
    Ann N Y Acad Sci 1141:58-75. 2008
    ....
  25. doi request reprint FOXO3a elicits a pro-apoptotic transcription program and cellular response to human lung carcinogen nicotine-derived nitrosaminoketone (NNK)
    Daniel C Blake
    Department of Pharmacology, Penn State University College of Medicine, Hershey, PA 17033, USA
    Lung Cancer 67:37-47. 2010
    ..This suggests that FOXO3a is a potential suppressor of carcinogenic damage in LAC...
  26. doi request reprint Plasma biomarkers in pediatric patients undergoing cardiopulmonary bypass
    Melinda E Lull
    Department of Pharmacology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Pediatr Res 63:638-44. 2008
    ....
  27. ncbi request reprint Dual-platform proteomics study of plasma biomarkers in pediatric patients undergoing cardiopulmonary bypass
    Todd M Umstead
    Department of Pediatrics, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Pediatr Res 67:641-9. 2010
    ..The changes in levels of expression of these proteins may provide insight into the understanding, treatment, and prevention of systemic inflammation, thereby helping to improve the outcomes of pediatric CPB patients...
  28. ncbi request reprint Plasma proteomics: a noninvasive window on pathology and pediatric cardiac surgery
    Melinda E Lull
    Department of Pharmacology, Penn State College of Medicine, Hershey, Pennsylvania 17033 0850, USA
    ASAIO J 52:562-6. 2006
    ..Of the detected spots, 79 (5.7%) were altered by CPB. These data illuminate the strength of plasma proteomics in identification of candidate biomarkers of CPB-associated disease processes...
  29. doi request reprint Twenty-five years of quantitative PCR for gene expression analysis
    Heather D VanGuilder
    Department of Pharmacology, Penn State College of Medicine, Hershey, PA, USA
    Biotechniques 44:619-26. 2008
    ..The future of qPCR remains bright as the technology becomes more rapid, cost-effective, easier to use, and capable of higher throughput...
  30. pmc The retinal proteome in experimental diabetic retinopathy: up-regulation of crystallins and reversal by systemic and periocular insulin
    Patrice E Fort
    Department of Ophthalmology, Penn State College of Medicine, Hershey, Pennsylvania 17033, USA
    Mol Cell Proteomics 8:767-79. 2009
    ..Altogether this work shows the importance of proteomics discovery methods coupled with targeted approaches to unveil new disease mechanistic details and therapeutic targets...
  31. pmc The use of neuroproteomics in drug abuse research
    Melinda E Lull
    Department of Pharmacology, Penn State College of Medicine, 500 University Drive, Hershey, PA 17033, USA
    Drug Alcohol Depend 107:11-22. 2010
    ....
  32. ncbi request reprint Diabetes downregulates presynaptic proteins and reduces basal synapsin I phosphorylation in rat retina
    Heather D VanGuilder
    The Penn State Retina Research Group, Ulerich Ophthalmology Research Laboratory, Department of Ophthalmology, Penn State College of Medicine, Milton S Hershey Medical Center, 500 University Drive, Hershey, PA 17033, USA
    Eur J Neurosci 28:1-11. 2008
    ....
  33. doi request reprint Human embryonic and mesenchymal stem cells express different nuclear proteomes
    Amritha Jaishankar
    Department of Pharmacology, Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA
    Stem Cells Dev 18:793-802. 2009
    ..This difference in the expression of Reptin52 and Pontin52 (known regulators of beta-catenin) further supports a role for Wnt signaling in stem cell self-renewal and proliferation...
  34. ncbi request reprint Repeated cocaine self-administration causes multiple changes in rat frontal cortex gene expression
    Willard M Freeman
    Center for the Neurobiological Investigation of Drug Abuse, Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston Salem NC 27157 1083, USA
    Neurochem Res 27:1181-92. 2002
    ..These changes share commonalities and exhibit differences with previous reports of gene expression changes in the frontal cortex after noncontingent cocaine administration...
  35. ncbi request reprint Systematic screening of gene expression using a cDNA macroarray
    Travis J Worst
    Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston Salem, NC, USA
    Methods Mol Med 79:243-59. 2003
  36. ncbi request reprint An interactive database of cocaine-responsive gene expression
    Willard M Freeman
    Center for the Neurobiological Investigation of Drug Abuse, Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Medical Center Blvd, Winston Salem, NC 27157 1083, USA
    ScientificWorldJournal 2:701-6. 2002
    ..Furthermore, this method of organizing large volumes of scientific information can easily be adapted to assist researchers in fields outside of drug abuse...
  37. ncbi request reprint Transcriptome analysis of frontal cortex in alcohol-preferring and nonpreferring rats
    Travis J Worst
    Center for the Neurobehavioral Study of Alcohol, Department of Physiology and Pharmacology, Wake Forest University Health Sciences, Winston Salem, North Carolina, USA
    J Neurosci Res 80:529-38. 2005
    ..Such alterations, however, might not be a universal characteristic of all animal models of alcohol abuse and will also require further investigation in post-mortem human samples...
  38. ncbi request reprint Changes in rat frontal cortex gene expression following chronic cocaine
    Willard M Freeman
    Center for the Neurobiological Investigation of Drug Abuse, Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, NC 27157 1083, USA
    Brain Res Mol Brain Res 104:11-20. 2002
    ..This study also validates the use of hybridization arrays for screening of neuronal gene expression changes and the utility of relative protein quantification as a post-hoc confirmation tool...
  39. ncbi request reprint Induction of GADD45 and GADD153 in neuroblastoma cells by dopamine-induced toxicity
    Alan H Stokes
    Department of Physiology and Pharmacology, Center for Neurobiological Investigation of Drug Abuse, Wake Forest University School of Medicine, Winston Salem, NC 27157 1083, USA
    Neurotoxicology 23:675-84. 2002
    ..Other genes that displayed changes, but that have not been subjected to post-hoc confirmation, include clusterin (increased), ubiquitin (increased), CD27 ligand (increased), CD27BP (increased), and rac-PK-beta (decreased)...
  40. ncbi request reprint Functional genomic analysis in pain research using hybridization arrays
    Stephen J Walker
    Center for the Neurobiological Investigation of Drug Abuse and Neurobehavioral Study of Alcohol, Wake Forest University School of Medicine, Winston Salem, NC, USA
    Methods Mol Med 99:239-53. 2004
    ..The experimental description in this chapter explains, in detail, how to perform a hybridization array using the macroarray platform...

Research Grants1

  1. Gene expression and monoamine transporter reversal.
    Willard Freeman; Fiscal Year: 2002
    ..In summary, the proposed studies will allow investigation of transcriptional responses to monoamine reuptake reversal in behaviorally relevant settings. ..