Research Topics
| W M FreemanSummaryAffiliation: Pennsylvania State University Country: USA Publications
Research Grants
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Detail Information
Publications
Proteomics for protein expression profiling in neuroscienceWillard M Freeman
Department of Pharmacology, Yerkes National Primate Research Center, Neuroscience Division, Emory University School of Medicine, Atlanta, Georgia 30329, USA
Neurochem Res 29:1065-81. 2004..Although there are many challenges in proteomic neuroscience, this field promises many rewards in the future...
Future prospects for biomarkers of alcohol consumption and alcohol-induced disordersWillard M Freeman
Department of Pharmacology, Penn State College of Medicine, Hershey, Pennsylvania 17033, USA
Alcohol Clin Exp Res 34:946-54. 2010..We recommend that NIAAA consider taking a leadership role in organizing investigators in the field and providing a common set of clinical specimens for biomarker validation studies...- Differences in the BAL proteome after Klebsiella pneumoniae infection in wild type and SP-A-/- miceMehboob Ali
Penn State Center for Host defense, Inflammation, and Lung Disease CHILD Research, Hershey, PA 17033, USA
Proteome Sci 8:34. 2010..Mice lacking SP-A are more susceptible to infection than wild type C57BL/6 mice. We studied bronchoalveolar lavage (BAL) protein expression in wild type and SP-A-/- mice infected with Klebsiella pneumoniae by 2D-DIGE... - The impact of surfactant protein-A on ozone-induced changes in the mouse bronchoalveolar lavage proteomeRizwanul Haque
Penn State Center for Host defense, Inflammation, and Lung Disease CHILD Research and the Department of Pediatrics, Penn State College of Medicine, Hershey, PA, USA
Proteome Sci 7:12. 2009.... - Chronic insulin treatment of diabetes does not fully normalize alterations in the retinal transcriptomeGeorgina V Bixler
Department of Pharmacology, Penn State College of Medicine, Hershey, PA, USA
BMC Med Genomics 4:40. 2011..The goal of this study was to identify molecular changes in the rodent retina induced by diabetes that are not normalized by insulin replacement and restoration of euglycemia... - Concurrent hippocampal induction of MHC II pathway components and glial activation with advanced aging is not correlated with cognitive impairmentHeather D VanGuilder
Department of Pharmacology, Pennsylvania State University College of Medicine, 500 University Drive, Hershey, Pennsylvania 17057, USA
J Neuroinflammation 8:138. 2011..This study sought to comprehensively investigate expression of the MHC II immune response pathway and glial activation in the hippocampus in the context of both aging and age-related cognitive decline... - Cognitive performance and age-related changes in the hippocampal proteomeW M Freeman
Department of Pharmacology, R130, Hershey Center for Applied Research, Penn State College of Medicine, 500 University Drive, Hershey, PA 17033, USA
Neuroscience 159:183-95. 2009.... - Gene expression changes in the medial prefrontal cortex and nucleus accumbens following abstinence from cocaine self-administrationWillard M Freeman
Department of Pharmacology, Penn State College of Medicine, Hershey, PA 17033, USA
BMC Neurosci 11:29. 2010..Persistent changes in gene expression, as well as alterations induced during abstinence may underlie long-lasting drug craving and relapse liability... - Persistent alterations in mesolimbic gene expression with abstinence from cocaine self-administrationWillard M Freeman
Department of Pharmacology, Penn State College of Medicine, Hershey, PA 17033, USA
Neuropsychopharmacology 33:1807-17. 2008..This incubation is accompanied by changes in gene expression that persist long after cessation of drug administration and may be regulated by chromatin remodeling... - Depletion of abundant proteins from non-human primate serum for biomarker studiesWillard M Freeman
Department of Pharmacology H078, Penn State College of Medicine, 500 University Drive, Hershey, PA 17033, USA
Proteomics 6:3109-13. 2006..Depleted serum also demonstrated greater sensitivity for previously masked, lower-abundance proteins... - Apo-AII is an elevated biomarker of chronic non-human primate ethanol self-administrationWillard M Freeman
Department of Pharmacology, H078, Penn State College of Medicine, 500 University Drive, PO Box 850, Hershey, PA 17033 0850, USA
Alcohol Alcohol 41:300-5. 2006.... - Distinct proteomic profiles of amphetamine self-administration transitional statesW M Freeman
Department of Pharmacology, Penn State College of Medicine, Hershey, PA 17033, USA
Pharmacogenomics J 5:203-14. 2005..These data provide the first classification of addiction-related behaviors based on clustering of quantitative proteomic measurements. ... 
A multistep validation process of biomarkers for preclinical drug developmentW M Freeman
Functional Genomics Core Facility, Penn State College of Medicine, Hershey, PA 17033, USA
Pharmacogenomics J 10:385-95. 2010....- Alterations in ionotropic glutamate receptor subunits during binge cocaine self-administration and withdrawal in ratsWenxue Tang
Department of Pharmacology, Yerkes National Primate Research Center, Neuroscience Division, Emory University School of Medicine, Atlanta, Georgia 30329, USA
J Neurochem 89:1021-33. 2004..Because subunit composition determines the functional properties of iGluRs, the observed changes may indicate alterations in the excitability of dopamine transmission underlying long-term biochemical and behavioral effects of cocaine... - Gene expression changes following extinction testing in a heroin behavioral incubation modelKara L Kuntz-Melcavage
Department of Pharmacology, Pennsylvania State University College of Medicine, Hershey, PA, USA
BMC Neurosci 10:95. 2009..Extinction sessions were conducted on groups after either 1 d or 14 d of drug-free enforced abstinence to demonstrate behavioral incubation... - Effects of ischemic preconditioning and bevacizumab on apoptosis and vascular permeability following retinal ischemia-reperfusion injurySTEVEN F ABCOUWER
Department of Surgery, Penn State College of Medicine, Hershey, Milton S Hershey Medical Center, Hershey, PA 17033 0850, USA
Invest Ophthalmol Vis Sci 51:5920-33. 2010.... - Gene expression profiles in HPV-immortalized human cervical cells treated with the nicotine-derived carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanoneBogdan Prokopczyk
Department of Pharmacology, Penn State College of Medicine, 500 University Drive, Hershey, PA 17033, USA
Chem Biol Interact 177:173-80. 2009..The results support previous epidemiological observations that, in addition to HPV, tobacco smoking also plays an important role in the development of cervical cancer... - Heroin self-administration: II. CNS gene expression following withdrawal and cue-induced drug-seeking behaviorKARA L KUNTZ
Department of Pharmacology, Pennsylvania State University College of Medicine, R130, 500 University Drive, Hershey, PA 17033, USA
Pharmacol Biochem Behav 90:349-56. 2008..These latter observations of persistent changes in gene expression following abstinence may reflect molecular correlates of relapse liability... - Clinical application for the preservation of phospho-proteins through in-situ tissue stabilizationC Bart Rountree
Department of Pediatrics, Penn State Hershey Medical Center, 500 University Drive, Hershey, PA 17033
Proteome Sci 8:61. 2010..Challenges to the use of protein biomarkers include inter-sample variability introduced by differences in post-collection handling and ex vivo degradation of proteins and protein modifications... - FOXO3a elicits a pro-apoptotic transcription program and cellular response to human lung carcinogen nicotine-derived nitrosaminoketone (NNK)Daniel C Blake
Department of Pharmacology, Penn State University College of Medicine, Hershey, PA 17033, USA
Lung Cancer 67:37-47. 2010..This suggests that FOXO3a is a potential suppressor of carcinogenic damage in LAC... - Classification of alcohol abuse by plasma protein biomarkersWillard M Freeman
Department of Pharmacology, Penn State College of Medicine, Hershey, Pennsylvania, USA
Biol Psychiatry 68:219-22. 2010..Previously, proposed single-analyte biochemical tests of alcohol intake suffer from low sensitivity and specificity or examine only acute drinking and have therefore seen limited clinical use... - Plasma biomarkers in pediatric patients undergoing cardiopulmonary bypassMelinda E Lull
Department of Pharmacology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
Pediatr Res 63:638-44. 2008.... - Correlating human and animal studies of cocaine abuse and gene expressionMelinda E Lull
Department of Pharmacology, Pennsylvania State College of Medicine, Hershey, PA 17033, USA
Ann N Y Acad Sci 1141:58-75. 2008.... - The retinal proteome in experimental diabetic retinopathy: up-regulation of crystallins and reversal by systemic and periocular insulinPatrice E Fort
Department of Ophthalmology, Penn State College of Medicine, Hershey, Pennsylvania 17033, USA
Mol Cell Proteomics 8:767-79. 2009..Altogether this work shows the importance of proteomics discovery methods coupled with targeted approaches to unveil new disease mechanistic details and therapeutic targets... - Plasma proteomics: a noninvasive window on pathology and pediatric cardiac surgeryMelinda E Lull
Department of Pharmacology, Penn State College of Medicine, Hershey, Pennsylvania 17033-0850, USA
ASAIO J 52:562-6. 2006..Of the detected spots, 79 (5.7%) were altered by CPB. These data illuminate the strength of plasma proteomics in identification of candidate biomarkers of CPB-associated disease processes... - Twenty-five years of quantitative PCR for gene expression analysisHeather D VanGuilder
Department of Pharmacology, Penn State College of Medicine, Hershey, PA, USA
Biotechniques 44:619-26. 2008..The future of qPCR remains bright as the technology becomes more rapid, cost-effective, easier to use, and capable of higher throughput... - Dual-platform proteomics study of plasma biomarkers in pediatric patients undergoing cardiopulmonary bypassTodd M Umstead
Department of Pediatrics, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
Pediatr Res 67:641-9. 2010..The changes in levels of expression of these proteins may provide insight into the understanding, treatment, and prevention of systemic inflammation, thereby helping to improve the outcomes of pediatric CPB patients... - The use of neuroproteomics in drug abuse researchMelinda E Lull
Department of Pharmacology, Penn State College of Medicine, 500 University Drive, Hershey, PA 17033, USA
Drug Alcohol Depend 107:11-22. 2010.... - Human embryonic and mesenchymal stem cells express different nuclear proteomesAmritha Jaishankar
Department of Pharmacology, Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA
Stem Cells Dev 18:793-802. 2009..This difference in the expression of Reptin52 and Pontin52 (known regulators of beta-catenin) further supports a role for Wnt signaling in stem cell self-renewal and proliferation... - Diabetes downregulates presynaptic proteins and reduces basal synapsin I phosphorylation in rat retinaHeather D VanGuilder
The Penn State Retina Research Group, Ulerich Ophthalmology Research Laboratory, Department of Ophthalmology, Penn State College of Medicine, Milton S Hershey Medical Center, 500 University Drive, Hershey, PA 17033, USA
Eur J Neurosci 28:1-11. 2008.... - Repeated cocaine self-administration causes multiple changes in rat frontal cortex gene expressionWillard M Freeman
Center for the Neurobiological Investigation of Drug Abuse, Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston Salem NC 27157 1083, USA
Neurochem Res 27:1181-92. 2002..These changes share commonalities and exhibit differences with previous reports of gene expression changes in the frontal cortex after noncontingent cocaine administration... - Systematic screening of gene expression using a cDNA macroarrayTravis J Worst
Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston-Salem, NC, USA
Methods Mol Med 79:243-59. 2003 - An interactive database of cocaine-responsive gene expressionWillard M Freeman
Center for the Neurobiological Investigation of Drug Abuse, Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Medical Center Blvd, Winston Salem, NC 27157 1083, USA
ScientificWorldJournal 2:701-6. 2002..Furthermore, this method of organizing large volumes of scientific information can easily be adapted to assist researchers in fields outside of drug abuse... - Transcriptome analysis of frontal cortex in alcohol-preferring and nonpreferring ratsTravis J Worst
Center for the Neurobehavioral Study of Alcohol, Department of Physiology and Pharmacology, Wake Forest University Health Sciences, Winston Salem, North Carolina, USA
J Neurosci Res 80:529-38. 2005..Such alterations, however, might not be a universal characteristic of all animal models of alcohol abuse and will also require further investigation in post-mortem human samples... - Changes in rat frontal cortex gene expression following chronic cocaineWillard M Freeman
Center for the Neurobiological Investigation of Drug Abuse, Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, NC 27157 1083, USA
Brain Res Mol Brain Res 104:11-20. 2002..This study also validates the use of hybridization arrays for screening of neuronal gene expression changes and the utility of relative protein quantification as a post-hoc confirmation tool... - Induction of GADD45 and GADD153 in neuroblastoma cells by dopamine-induced toxicityAlan H Stokes
Department of Physiology and Pharmacology, Center for Neurobiological Investigation of Drug Abuse, Wake Forest University School of Medicine, Winston-Salem, NC 27157-1083, USA
Neurotoxicology 23:675-84. 2002..Other genes that displayed changes, but that have not been subjected to post-hoc confirmation, include clusterin (increased), ubiquitin (increased), CD27 ligand (increased), CD27BP (increased), and rac-PK-beta (decreased)... - Functional genomic analysis in pain research using hybridization arraysStephen J Walker
Center for the Neurobiological Investigation of Drug Abuse and Neurobehavioral Study of Alcohol, Wake Forest University School of Medicine, Winston-Salem, NC, USA
Methods Mol Med 99:239-53. 2004..The experimental description in this chapter explains, in detail, how to perform a hybridization array using the macroarray platform...
Research Grants
- Gene expression and monoamine transporter reversal.Willard Freeman; Fiscal Year: 2002..In summary, the proposed studies will allow investigation of transcriptional responses to monoamine reuptake reversal in behaviorally relevant settings. ..