Joanna Floros

Summary

Affiliation: Pennsylvania State University
Country: USA

Publications

  1. pmc Genetic variants of surfactant proteins A, B, C, and D in bronchopulmonary dysplasia
    J Pavlovic
    Department of Cellular and Molecular Physiology, PSU, Hershey, PA 17033, USA
    Dis Markers 22:277-91. 2006
  2. pmc Sex differences in the response of the alveolar macrophage proteome to treatment with exogenous surfactant protein-A
    David S Phelps
    Center for Host defense, Inflammation, and Lung Disease CHILD Research and Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA
    Proteome Sci 10:44. 2012
  3. pmc IL-18R1 and IL-18RAP SNPs may be associated with bronchopulmonary dysplasia in African-American infants
    Joanna Floros
    Department of Pediatrics and CHILD Research, Penn State College of Medicine, Hershey, Pennsylvania, USA
    Pediatr Res 71:107-14. 2012
  4. ncbi request reprint Human surfactant protein A (SP-A) variants: why so many, why such a complexity?
    J Floros
    Departments of Cellular and Molecular Physiology and Pediatrics, Pennsylvania State University College of Medicine, Hershey, PA, USA
    Swiss Med Wkly 131:87-90. 2001
  5. pmc Surfactant protein genetics in community-acquired pneumonia: balancing the host inflammatory state
    Joanna Floros
    Department of Pediatrics, Center for Host defense, Inflammation, and Lung Disease Research, 500 University Drive, H085, Hershey, PA 17033, USA
    Crit Care 15:156. 2011
  6. pmc Surfactant protein (SP) B associations and interactions with SP-A in white and black subjects with respiratory distress syndrome
    J Floros
    Department of Cellular and Molecular Physiology, The Milton S Hershey Medical Center, Pennsylvania State University College of Medicine, PO Box 850, 500 University Drive, Hershey, PA 17033, USA
    Pediatr Int 43:567-76. 2001
  7. ncbi request reprint Family-based transmission disequilibrium test (TDT) and case-control association studies reveal surfactant protein A (SP-A) susceptibility alleles for respiratory distress syndrome (RDS) and possible race differences
    J Floros
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Clin Genet 60:178-87. 2001
  8. ncbi request reprint Family-based association tests suggest linkage between surfactant protein B (SP-B) (and flanking region) and respiratory distress syndrome (RDS): SP-B haplotypes and alleles from SP-B-linked loci are risk factors for RDS
    Joanna Floros
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Pediatr Res 59:616-21. 2006
  9. pmc In vivo rescue of alveolar macrophages from SP-A knockout mice with exogenous SP-A nearly restores a wild type intracellular proteome; actin involvement
    David S Phelps
    Center for Host defense, Inflammation, and Lung Disease CHILD Research and Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Proteome Sci 9:67. 2011
  10. ncbi request reprint A point of view: quantitative and qualitative imbalance in disease pathogenesis; pulmonary surfactant protein A genetic variants as a model
    J Floros
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, 500 University Drive, H166, Hershey, PA 17033 0850, USA
    Comp Biochem Physiol A Mol Integr Physiol 129:295-303. 2001

Collaborators

Detail Information

Publications73

  1. pmc Genetic variants of surfactant proteins A, B, C, and D in bronchopulmonary dysplasia
    J Pavlovic
    Department of Cellular and Molecular Physiology, PSU, Hershey, PA 17033, USA
    Dis Markers 22:277-91. 2006
    ..The data indicate that SP loci are linked to BPD. Studies in different study groups and/or of larger sample size are warranted to confirm these observations and delineate genetic background of BPD subgroups...
  2. pmc Sex differences in the response of the alveolar macrophage proteome to treatment with exogenous surfactant protein-A
    David S Phelps
    Center for Host defense, Inflammation, and Lung Disease CHILD Research and Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA
    Proteome Sci 10:44. 2012
    ..abstract:..
  3. pmc IL-18R1 and IL-18RAP SNPs may be associated with bronchopulmonary dysplasia in African-American infants
    Joanna Floros
    Department of Pediatrics and CHILD Research, Penn State College of Medicine, Hershey, Pennsylvania, USA
    Pediatr Res 71:107-14. 2012
    ..The genetic contribution to the development of bronchopulmonary dysplasia (BPD) in prematurely born infants is substantial, but information related to the specific genes involved is lacking...
  4. ncbi request reprint Human surfactant protein A (SP-A) variants: why so many, why such a complexity?
    J Floros
    Departments of Cellular and Molecular Physiology and Pediatrics, Pennsylvania State University College of Medicine, Hershey, PA, USA
    Swiss Med Wkly 131:87-90. 2001
    ..Moreover, understanding of such a complexity may be useful in the study of disease pathogenesis and the development of disease diagnostics and/or therapeutics...
  5. pmc Surfactant protein genetics in community-acquired pneumonia: balancing the host inflammatory state
    Joanna Floros
    Department of Pediatrics, Center for Host defense, Inflammation, and Lung Disease Research, 500 University Drive, H085, Hershey, PA 17033, USA
    Crit Care 15:156. 2011
    ..Understanding the maintenance of the balance of the inflammatory state may be one of the keys to understanding pulmonary disease progression...
  6. pmc Surfactant protein (SP) B associations and interactions with SP-A in white and black subjects with respiratory distress syndrome
    J Floros
    Department of Cellular and Molecular Physiology, The Milton S Hershey Medical Center, Pennsylvania State University College of Medicine, PO Box 850, 500 University Drive, Hershey, PA 17033, USA
    Pediatr Int 43:567-76. 2001
    ..The etiology of respiratory distress syndrome (RDS) is multifactorial and/or multigenic. Surfactant protein A (SP-A) and/or SP-B genetic variants have been identified as risk or protection factors for RDS...
  7. ncbi request reprint Family-based transmission disequilibrium test (TDT) and case-control association studies reveal surfactant protein A (SP-A) susceptibility alleles for respiratory distress syndrome (RDS) and possible race differences
    J Floros
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Clin Genet 60:178-87. 2001
    ..Some differences between blacks and whites with regard to SP-A alleles may exist...
  8. ncbi request reprint Family-based association tests suggest linkage between surfactant protein B (SP-B) (and flanking region) and respiratory distress syndrome (RDS): SP-B haplotypes and alleles from SP-B-linked loci are risk factors for RDS
    Joanna Floros
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Pediatr Res 59:616-21. 2006
    ..We conclude that SP-B or SP-B-linked loci are linked to RDS and certain alleles or haplotypes are susceptibility or protective factors for the development of RDS in infants born prematurely...
  9. pmc In vivo rescue of alveolar macrophages from SP-A knockout mice with exogenous SP-A nearly restores a wild type intracellular proteome; actin involvement
    David S Phelps
    Center for Host defense, Inflammation, and Lung Disease CHILD Research and Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Proteome Sci 9:67. 2011
    ..abstract:..
  10. ncbi request reprint A point of view: quantitative and qualitative imbalance in disease pathogenesis; pulmonary surfactant protein A genetic variants as a model
    J Floros
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, 500 University Drive, H166, Hershey, PA 17033 0850, USA
    Comp Biochem Physiol A Mol Integr Physiol 129:295-303. 2001
    ....
  11. ncbi request reprint Surfactant protein A and B genetic variants and respiratory distress syndrome: allele interactions
    J Floros
    Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Biol Neonate 80:22-5. 2001
    ..In this paper we summarize allele associations and discuss allele interaction of the surfactant protein genes in relation to RDS (the term allele and genetic variant will be used interchangeably)...
  12. ncbi request reprint Surfactant protein genetic marker alleles identify a subgroup of tuberculosis in a Mexican population
    J Floros
    Dept of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    J Infect Dis 182:1473-8. 2000
    ..Moreover, interactions were seen between alleles 6A(2) and 1A(3) (P=.0064) and between 1A(3) and B1013_A (P=. 036). The findings indicate a possible involvement of SP alleles in tuberculosis pathogenesis...
  13. pmc Genetic complexity of the human innate host defense molecules, surfactant protein A1 (SP-A1) and SP-A2--impact on function
    Joanna Floros
    The Penn State University Center for Host Defense, Inflammation, and Lung Disease CHILD Research, Department of Pediatrics, College of Medicine, The Pennsylvania State University, Hershey, PA 17033, USA
    Crit Rev Eukaryot Gene Expr 19:125-37. 2009
    ..In this review, we discuss the human SP-A complexity and how this may affect SP-A function...
  14. ncbi request reprint Haplotypes of the surfactant protein genes A and D as susceptibility factors for the development of respiratory distress syndrome
    Neal J Thomas
    Department of Pediatrics, Penn State Children s Hospita, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Acta Paediatr 96:985-9. 2007
    ....
  15. pmc Humanized SFTPA1 and SFTPA2 transgenic mice reveal functional divergence of SP-A1 and SP-A2: formation of tubular myelin in vivo requires both gene products
    Guirong Wang
    Penn State Center for Host defense, Inflammation, and Lung Disease Research, Department of Pediatrics, Penn State HersheyCollege ofMedicine, Pennsylvania State University, Hershey, Pennsylvania 17033, USA
    J Biol Chem 285:11998-2010. 2010
    ..These observations indicate that SP-A1 and SP-A2 diverged functionally at least in terms of TM formation...
  16. pmc Surfactant protein A2 (SP-A2) variants expressed in CHO cells stimulate phagocytosis of Pseudomonas aeruginosa more than do SP-A1 variants
    Anatoly N Mikerov
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA
    Infect Immun 75:1403-12. 2007
    ..We conclude that SP-A2 variants stimulate phagocytosis of P. aeruginosa more effectively than SP-A1 variants and that posttranslational modifications positively influence the phagocytic activity of SP-A...
  17. pmc Impact of ozone exposure on the phagocytic activity of human surfactant protein A (SP-A) and SP-A variants
    Anatoly N Mikerov
    Dept of Cellular and Molecular Physiology, The Pennsylvania State Univ College of Medicine, 500 University Dr Hershey, PA 17033, USA
    Am J Physiol Lung Cell Mol Physiol 294:L121-30. 2008
    ..We speculate that functional differences between SP-A1 and SP-A2 exist in vivo and that the redox status of the lung microenvironment differentially affects function of SP-A1 and SP-A2...
  18. pmc Impact of sex and ozone exposure on the course of pneumonia in wild type and SP-A (-/-) mice
    Anatoly N Mikerov
    Center for Host defense, Inflammation, and Lung Disease CHILD Research, Department of Pediatrics, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey H085, PA 17033, USA
    Microb Pathog 52:239-49. 2012
    ....
  19. pmc Transmission of surfactant protein variants and haplotypes in children hospitalized with respiratory syncytial virus
    Neal J Thomas
    Department of Pediatrics, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Pediatr Res 66:70-3. 2009
    ..Further study is required to determine whether these variants can be used to target a high-risk patient population in clinical trials aimed at reducing either the symptoms of acute infection or long-term pulmonary sequelae...
  20. ncbi request reprint Surfactant protein gene A, B, and D marker alleles in chronic obstructive pulmonary disease of a Mexican population
    X Guo
    Dept of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey 17033, USA
    Eur Respir J 18:482-90. 2001
    ..These observations should now be confirmed in a larger study, designed according to strict epidemiological criteria...
  21. ncbi request reprint DNA methylation markers of surfactant proteins in lung cancer
    Zhenwu Lin
    Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Int J Oncol 31:181-91. 2007
    ..We speculate that SP-A1 and SP-D are subject to methylation/demethylation regulatory mechanisms and are involved in lung cancer pathogenesis by virtue of their function in innate host defense and/or regulation of inflammation...
  22. ncbi request reprint Human SP-A genetic variants and bleomycin-induced cytokine production by THP-1 cells: effect of ozone-induced SP-A oxidation
    Weixiong Huang
    Departments of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Am J Physiol Lung Cell Mol Physiol 286:L546-53. 2004
    ..The results indicate that differences among SP-A variants may partly explain the individual variability of pulmonary complications observed during bleomycin chemotherapy and/or in an environment that may promote protein oxidation...
  23. pmc Ablation of SP-A has a negative impact on the susceptibility of mice to Klebsiella pneumoniae infection after ozone exposure: sex differences
    Anatoly N Mikerov
    The Penn State Center for Host defense, Inflammation, and Lung Disease Research, Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Respir Res 9:77. 2008
    ..We hypothesized that ablation of SP-A has a negative impact on the susceptibility of mice to Klebsiella pneumoniae infection after ozone exposure, and that sex differences in the effect of ozone do exist...
  24. pmc Effect of cysteine 85 on biochemical properties and biological function of human surfactant protein A variants
    Guirong Wang
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Biochemistry 46:8425-35. 2007
    ..Residue 85 plays an important role in the structure and function of SP-A and is a major factor for the differences between SP-A1 and SP-A2 variants...
  25. pmc The untranslated exon B of human surfactant protein A2 mRNAs is an enhancer for transcription and translation
    Patricia Silveyra
    Center for Host defense, Inflammation, and Lung Disease Research, Department of Pediatrics, Hershey, Pennsylvania 17033 0850, USA
    Am J Physiol Lung Cell Mol Physiol 301:L795-803. 2011
    ..We conclude that eB sequence and length are determinants of transcription and translation efficiency...
  26. pmc Human SP-A1 (SFTPA1) variant-specific 3' UTRs and poly(A) tail differentially affect the in vitro translation of a reporter gene
    Patricia Silveyra
    Penn State Center for Host defense, Inflammation, and Lung Disease Research, Department of Pediatrics, Pennsylvania State University, Hershey, USA
    Am J Physiol Lung Cell Mol Physiol 299:L523-34. 2010
    ..These results indicate that sequence differences among hSP-A gene variants may account for differential regulation at the translational level...
  27. ncbi request reprint Polymorphisms of human SP-A, SP-B, and SP-D genes: association of SP-B Thr131Ile with ARDS
    Z Lin
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey 17033, USA
    Clin Genet 58:181-91. 2000
    ..These data suggest that SP-B or a linked gene contributes to susceptibility to ARDS...
  28. pmc Gonadal hormones and oxidative stress interaction differentially affects survival of male and female mice after lung Klebsiella pneumoniae infection
    Faryal Durrani
    Center for Host defense, Inflammation, and Lung Disease CHILD, Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA 17033 0850, USA
    Exp Lung Res 38:165-72. 2012
    ..These findings indicate that gonadal hormones and/or oxidative stress have a significant effect on mouse survival...
  29. pmc Cap-independent translation of human SP-A 5'-UTR variants: a double-loop structure and cis-element contribution
    Guirong Wang
    Department of Pediatrics, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Am J Physiol Lung Cell Mol Physiol 296:L635-47. 2009
    ..Thus several factors, including cis-elements and secondary structure type and stability, are required for hSP-A 5'-UTR variant-mediated cap-independent translation...
  30. ncbi request reprint SP-A1 and SP-A2 variants differentially enhance association of Pseudomonas aeruginosa with rat alveolar macrophages
    Anatoly N Mikerov
    Dept of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Am J Physiol Lung Cell Mol Physiol 288:L150-8. 2005
    ..aeruginosa with rat alveolar macrophages differentially and in a concentration-dependent manner, with SP-A2 variants having a higher activity compared with SP-A1 variants...
  31. pmc Deletions within a CA-repeat-rich region of intron 4 of the human SP-B gene affect mRNA splicing
    Zhenwu Lin
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Biochem J 389:403-12. 2005
    ..The results indicate that intron 4 length variants affect SP-B mRNA splicing, and that this may contribute to lung disease...
  32. ncbi request reprint Differential allele expression of host defense genes, pulmonary surfactant protein-A and osteopontin, in rat
    Zhenwu Lin
    Department of Cellular and Molecular Physiology, College of Medicine, The Pennsylvania State University, H166, 500 University Drive, Hershey, PA 17033, USA
    Mol Immunol 41:1155-65. 2004
    ..We conclude that allele-specific expression occurs among genes of innate host defense. This may yet provide another level of regulatory complexity for molecules involved in the first line of defense...
  33. pmc Study of human SP-A, SP-B and SP-D loci: allele frequencies, linkage disequilibrium and heterozygosity in different races and ethnic groups
    Wenlei Liu
    Department of Health Evaluation Sciences, Penn State College of Medicine, Hershey, PA 17033, USA
    BMC Genet 4:13. 2003
    ....
  34. pmc Sex differences in the impact of ozone on survival and alveolar macrophage function of mice after Klebsiella pneumoniae infection
    Anatoly N Mikerov
    The Penn State Center for Host defense, Inflammation, and Lung Disease CHILD Research, Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Respir Res 9:24. 2008
    ..The purpose of this study was to determine whether ozone exposure differentially affects: 1) survival of male and female mice infected with Klebsiella pneumoniae, and 2) the phagocytic ability of macrophages from these mice...
  35. ncbi request reprint Modulatory effects of ozone on THP-1 cells in response to SP-A stimulation
    Branislava Janic
    Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Am J Physiol Lung Cell Mol Physiol 288:L317-25. 2005
    ..These findings indicate that O(3) exposure may mediate its effect on macrophage function both directly and indirectly (via SP-A oxidation) and by involving different mechanisms...
  36. ncbi request reprint Combined SP-A-bleomycin effect on cytokines by THP-1 cells: impact of surfactant lipids on this effect
    Weixiong Huang
    Department of Cellular and Molecular Physiology, Pennsylvania State College of Medicine, Hershey, Pennsylvania 17033, USA
    Am J Physiol Lung Cell Mol Physiol 283:L94-L102. 2002
    ....
  37. ncbi request reprint Differences in biochemical properties and in biological function between human SP-A1 and SP-A2 variants, and the impact of ozone-induced oxidation
    Guirong Wang
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Biochemistry 43:4227-39. 2004
    ..v) The SP-A2 variant (1A(0)) and the coexpressed protein 1A(0)/6A(2) inhibit ATP-stimulated PC secretion from alveolar type II cells to a greater extent than SP-A1 (6A(2)), a biologic activity that was susceptible to ozone treatment...
  38. ncbi request reprint Characterization of a human surfactant protein A1 (SP-A1) gene-specific antibody; SP-A1 content variation among individuals of varying age and pulmonary health
    Hephzibah Rani S Tagaram
    Departments of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Am J Physiol Lung Cell Mol Physiol 292:L1052-63. 2007
    ..10) compared with culture-positive NCF (0.368 +/- 0.085). In summary, we developed and characterized an SP-A1 gene-specific antibody and used it to identify gene-specific SP-A content in BALFs as a function of age and lung health...
  39. ncbi request reprint Human SP-A protein variants derived from one or both genes stimulate TNF-alpha production in the THP-1 cell line
    G Wang
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Am J Physiol Lung Cell Mol Physiol 278:L946-54. 2000
    ..These data suggest that the SP-A-induced increases in TNF-alpha levels differ among SP-A variants and appear to be affected by SP-A genotype and whether SP-A is derived from one or both genes...
  40. pmc Differences in N-linked glycosylation between human surfactant protein-B variants of the C or T allele at the single-nucleotide polymorphism at position 1580: implications for disease
    Guirong Wang
    Department of Cellular and Molecular Physiology, The Pennsylvania State University, College of Medicine, Hershey, PA 17033, USA
    Biochem J 369:179-84. 2003
    ..Further, we speculate that, given the fact that this SNP is found frequently in the general population, N-linked glycosylation at residue Asn129 interferes with SP-B processing, secretion and folding under certain disease conditions...
  41. pmc The effect of ozone exposure on the ability of human surfactant protein a variants to stimulate cytokine production
    Guirong Wang
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Environ Health Perspect 110:79-84. 2002
    ....
  42. pmc Differentiation of xenografted human fetal lung parenchyma
    Jelena Pavlovic
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Early Hum Dev 84:181-93. 2008
    ..This model in which xenografted lung tissue in different stages of development is available may facilitate the study of human fetal lung development and the impact of various pharmacological agents on this process...
  43. ncbi request reprint Differences in the translation efficiency and mRNA stability mediated by 5'-UTR splice variants of human SP-A1 and SP-A2 genes
    Guirong Wang
    Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, 17033, USA
    Am J Physiol Lung Cell Mol Physiol 289:L497-508. 2005
    ..These findings indicate that the hSP-A 5'-UTR splice variants play an important role in both SP-A translation and mRNA stability...
  44. pmc Role of surfactant protein-A (SP-A) in lung injury in response to acute ozone exposure of SP-A deficient mice
    Rizwanul Haque
    Department of Pediatrics, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Toxicol Appl Pharmacol 220:72-82. 2007
    ..In summary, several aspects of the inflammatory response differ between WT and KO mice. These in vivo findings appear to implicate SP-A in regulating inflammation and limiting epithelial damage in response to ozone exposure...
  45. pmc Cyclic changes in the level of the innate immune molecule, surfactant protein-a, and cytokines in vaginal fluid
    Colin MacNeill
    Department of Obstetrics and Gynecology, Pennsylvania State University College of Medicine, Hershey, PA 17083, USA
    Am J Reprod Immunol 68:244-50. 2012
    ..Specifically, little is known about the pattern-recognition component of vaginal innate immunity, the relationship of pattern-recognition molecules to known cytokine levels, and the role of gonadal hormones in their regulation...
  46. ncbi request reprint Human SP-A 3'-UTR variants mediate differential gene expression in basal levels and in response to dexamethasone
    Guirong Wang
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Am J Physiol Lung Cell Mol Physiol 284:L738-48. 2003
    ..The data indicate that the 3'-UTR of SP-As play a differential role in SP-A basal expression and in response to Dex. Therefore, a careful consideration of individual use of steroid treatment may be considered...
  47. ncbi request reprint Regulation of expression of human SP-A1 and SP-A2 genes in fetal lung explant culture
    A M Karinch
    Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey 17033, USA
    Biochim Biophys Acta 1398:192-202. 1998
    ..We conclude that SP-A genotype may account in part for the marked differences in SP-A mRNA concentration among fetal lungs and that the SP-A genes and/or alleles may be differentially regulated...
  48. ncbi request reprint Heterogeneous allele expression of pulmonary SP-D gene in rat large intestine and other tissues
    Zhenwu Lin
    Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Physiol Genomics 11:235-43. 2002
    ..The parent-of-origin does not play a role in SP-D allele-specific expression. However, acquired epigenetic factors, family background, or other factors may contribute to the overall pattern of expression...
  49. pmc Differences in the BAL proteome after Klebsiella pneumoniae infection in wild type and SP-A-/- mice
    Mehboob Ali
    Penn State Center for Host defense, Inflammation, and Lung Disease CHILD Research, Hershey, PA 17033, USA
    Proteome Sci 8:34. 2010
    ..Mice lacking SP-A are more susceptible to infection than wild type C57BL/6 mice. We studied bronchoalveolar lavage (BAL) protein expression in wild type and SP-A-/- mice infected with Klebsiella pneumoniae by 2D-DIGE...
  50. pmc Genetic variants and monoallelic expression of surfactant protein-D in inflammatory bowel disease
    Zhenwu Lin
    Department of Surgery, Pennsylvania State University College of Medicine, Hershey, USA
    Ann Hum Genet 75:559-68. 2011
    ..The results suggest that differential allelic expression may affect penetrance of the SNP rs721917 disease-susceptibility allele in IBD. The potential impact of SP-D monoallelic expression on incomplete penetrance is discussed...
  51. pmc Inhibition of hemagglutination activity of influenza A viruses by SP-A1 and SP-A2 variants expressed in CHO cells
    Anatoly N Mikerov
    Department of Cellular and Molecular Physiology, H166, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA
    Med Microbiol Immunol 197:9-12. 2008
    ..Based on these findings, we speculate that in human lung SP-A1 and SP-A2 will not be different in their activity against IAV that preferably bind to alpha(2,3)-linked sialic acids (like avian strains)...
  52. pmc Genetic complexity of the human surfactant-associated proteins SP-A1 and SP-A2
    Patricia Silveyra
    Center for Host defense, Inflammation, and Lung Disease CHILD Research, Department of Pediatrics, Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA
    Gene 531:126-32. 2013
    ..The aim of this report is to describe the genetic complexity of the SFTPA1 and SFTPA2 genes, as well as to review regulatory mechanisms that control SP-A expression in humans and other animal species. ..
  53. pmc Genetic variant associations of human SP-A and SP-D with acute and chronic lung injury
    Patricia Silveyra
    Center for Host defense, Inflammation, and Lung Disease Research, Department of Pediatrics, Pennsylvania State University College of Medicine, Pennsylvania, USA
    Front Biosci (Landmark Ed) 17:407-29. 2012
    ..The potential role of genetic variations in pulmonary disease or pathogenesis is discussed following an evaluation, and comparison of the available literature...
  54. pmc Air pollution and epigenetics: effects on SP-A and innate host defence in the lung
    Patricia Silveyra
    Center for Host defense, Inflammation, and Lung Disease CHILD Research, Department of Pediatrics, Pennsylvania State University College of Medicine, Hershey, USA
    Swiss Med Wkly 142:w13579. 2012
    ..Their function may be differentially affected by pollutants and specifically by ozone-induced oxidative stress, and this in turn may differentially affect susceptibility to lung disease...
  55. pmc Surfactant protein A, an innate immune factor, is expressed in the vaginal mucosa and is present in vaginal lavage fluid
    Colin MacNeill
    Department of Obstetrics and Gynecology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Immunology 111:91-9. 2004
    ..A corollary hypothesis is that qualitative and quantitative alterations of normal SP-A may play a role in the pathogenesis of lower genital tract inflammatory conditions...
  56. ncbi request reprint An in vitro cell model system for the study of the effects of ozone and other gaseous agents on phagocytic cells
    Branislava Janic
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey 17033, USA
    J Immunol Methods 272:125-34. 2003
    ..This model system offers conditions where the experimental results are not due to cell death, but rather due to the effects of ozone and/or agents under investigation...
  57. ncbi request reprint Factors affecting SP-A-mediated phagocytosis in human monocytic cell lines
    Jianqiang Ding
    Department of Cellular Physiology, Pennsylvania State College of Medicine, P O Box 850, Hershey, PA 17033, USA
    Respir Med 98:637-50. 2004
    ..Moreover, the stimulatory effect of SP-A was pH-dependent and appeared to be independent of several other phagocytic mechanisms, including those mediated by Fc receptors and mannose receptor...
  58. pmc Surfactant protein DNA methylation: a new entrant in the field of lung cancer diagnostics? (Review)
    Mudit Vaid
    The Penn State Center for Host defense, Inflammation and Lung Disease CHILD Research, Department of Pediatrics, Penn State Hershey College of Medicine, Hershey, PA 17033, USA
    Oncol Rep 21:3-11. 2009
    ....
  59. ncbi request reprint Genetics of the hydrophilic surfactant proteins A and D
    J Floros
    Department of Cellular and Molecular Physiology H166, Pennsylvania State University College of Medicine, P O Box 850, 500 University Dr, Hershey, PA 17033, USA
    Biochim Biophys Acta 1408:312-22. 1998
    ..The evidence gathered thus far indicates that SP-A, possibly by interacting with other surfactant components, may play a role (e.g. be a susceptibility factor) in the development of respiratory disease...
  60. ncbi request reprint Both human SP-A1 and Sp-A2 genes are expressed in small and large intestine
    Z Lin
    Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey 17033 0850, USA
    Pediatr Pathol Mol Med 20:367-86. 2001
    ..We speculate that SP-A in the intestine plays a role in local host defense and inflammation...
  61. ncbi request reprint Surfactant proteins: molecular genetics of neonatal pulmonary diseases
    J Floros
    Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey 17033, USA
    Annu Rev Physiol 60:365-84. 1998
    ....
  62. pmc Exon B of human surfactant protein A2 mRNA, alone or within its surrounding sequences, interacts with 14-3-3; role of cis-elements and secondary structure
    Georgios T Noutsios
    Center for Host defense, Inflammation, and Lung Disease CHILD Research Department of Pediatrics, College of Medicine, The Pennsylvania State University, Hershey, PA 17033, USA
    Am J Physiol Lung Cell Mol Physiol 304:L722-35. 2013
    ..Differential regulation of SP-A1 and SP-A2 is mediated by the 14-3-3 protein family as well as by a number of other proteins that bind UTRs with or without eB mRNA...
  63. pmc The impact of surfactant protein-A on ozone-induced changes in the mouse bronchoalveolar lavage proteome
    Rizwanul Haque
    Penn State Center for Host defense, Inflammation, and Lung Disease CHILD Research and the Department of Pediatrics, Penn State College of Medicine, Hershey, PA, USA
    Proteome Sci 7:12. 2009
    ....
  64. pmc Differential effects of human SP-A1 and SP-A2 variants on phospholipid monolayers containing surfactant protein B
    Guirong Wang
    Department of Cellular and Molecular Physiology, H166, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA
    Biochim Biophys Acta 1768:2060-9. 2007
    ..The results indicate that human SP-A1 and SP-A2 variants exhibit differential effects on characteristics of phospholipid monolayers containing SP-B. This may differentially impact surface film activity...
  65. ncbi request reprint Human surfactant protein-C: genetic homogeneity and expression in RDS; comparison with other species
    D Hatzis
    Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey 17033 0850
    Exp Lung Res 20:57-72. 1994
    ....
  66. ncbi request reprint Sex differences in the acute in vivo effects of different human SP-A variants on the mouse alveolar macrophage proteome
    David S Phelps
    The Center for Host Defense, Inflammation, and Lung Disease CHILD Research, Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA, USA
    J Proteomics 108:427-44. 2014
    ..These observations not only demonstrate the therapeutic potential of exogenous SP-A, but also illustrate sex- and gene-specific differences in the response to it...
  67. ncbi request reprint An 11-nt sequence polymorphism at the 3'UTR of human SFTPA1 and SFTPA2 gene variants differentially affect gene expression levels and miRNA regulation in cell culture
    Patricia Silveyra
    Center for Host defense, Inflammation, and Lung Disease CHILD Research, Department of Pediatrics, Department of Biochemistry and Molecular Biology and
    Am J Physiol Lung Cell Mol Physiol 307:L106-19. 2014
    ..Our results indicate that sequence variability at the 3'UTR of SP-A variants differentially affects miRNA regulation of gene expression. ..
  68. ncbi request reprint Surfactant protein A and B genetic variants predispose to idiopathic pulmonary fibrosis
    Moises Selman
    Instituto Nacional de Enfermedades Respiratorias, Mexico DF, Mexico
    Hum Genet 113:542-50. 2003
    ..These findings indicate that surfactant protein variants may serve as markers to identify subgroups of patients at risk, and we speculate that these contribute to IPF pathogenesis...
  69. ncbi request reprint Inhibition of ozone-induced SP-A oxidation by plant polyphenols
    Dimitrios Stagos
    Department of Biochemistry, University of Thessaly, Larissa, Greece
    Free Radic Res 41:357-66. 2007
    ..The results indicate that inhibition of SP-A oxidation by plant polyphenols may be a mechanism accounting for the protective activity of natural antioxidants against the effects of ozone exposure on lungs...
  70. ncbi request reprint From birds to humans: new concepts on airways relative to alveolar surfactant
    Wolfgang Bernhard
    Department of Neonatology, Faculty of Medicine, Eberhard Karls University, Calwer Strasse 7, D 72076 Tubingen, Germany
    Am J Respir Cell Mol Biol 30:6-11. 2004
    ..We suggest that the molecular composition of surfactant is adapted to differences in the architecture of pulmonary surfaces and to the dynamics of surface area changes during respiration...
  71. ncbi request reprint Resident murine alveolar and peritoneal macrophages differ in adhesion of apoptotic thymocytes
    Bin Hu
    Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Health System, Ann Arbor, MI 48105 2303, USA
    Am J Respir Cell Mol Biol 30:687-93. 2004
    ..The decreased capacity of murine AMø to ingest apoptotic cells is only partially explained by reduced initial adhesion...
  72. pmc Surfactant protein B intron 4 variation in German patients with COPD and acute respiratory failure
    Carola Seifart
    Department of Internal Medicine, Division Respiratory and Critical Care Medicine, Philipps University of Marburg, Baldingerstasse, 35043 Marburg, Germany
    Dis Markers 18:129-36. 2002
    ..5%, p = 0.003, OR 4.9, 95% CI: 1.76-13.6). The data indicate that SP-B intron 4 variants may associate with increased risk of ARF in COPD and may be used as a marker of susceptibility in this disease subgroup...

Research Grants27

  1. SURFACTANT PROTEINS AND RESPIRATORY DISTRESS SYNDROME
    Joanna Floros; Fiscal Year: 2009
    ....
  2. OZONE EFFECTS ON FUNCTION OF SURFACTANT PROTEIN VARIANTS
    Joanna Floros; Fiscal Year: 2010
    ..Knowledge gained may help explain and perhaps link the in vitro data with the human genetic association data, and provide insight in the understanding of the value of the SP-A gene duplication. ..
  3. OZONE EFFECTS ON FUNCTION OF SURFACTANT PROTEIN VARIANTS
    Joanna Floros; Fiscal Year: 2004
    ..Knowledge gained may help explain and perhaps link the in vitro data with the human genetic association data, and provide insight in the understanding of the value of the SP-A gene duplication. ..
  4. The Role of surfactant protein genetic variants in cyst*
    Joanna Floros; Fiscal Year: 2005
    ..aeruginosa and its clearance, and provide the basis for further consideration of novel therapeutic strategies to treat CF-related lung disease. ..
  5. OZONE EFFECTS ON FUNCTION OF SURFACTANT PROTEIN VARIANTS
    Joanna Floros; Fiscal Year: 2005
    ..Knowledge gained may help explain and perhaps link the in vitro data with the human genetic association data, and provide insight in the understanding of the value of the SP-A gene duplication. ..
  6. OZONE EFFECTS ON FUNCTION OF SURFACTANT PROTEIN VARIANTS
    Joanna Floros; Fiscal Year: 2006
    ..Knowledge gained may help explain and perhaps link the in vitro data with the human genetic association data, and provide insight in the understanding of the value of the SP-A gene duplication. ..
  7. OZONE EFFECTS ON FUNCTION OF SURFACTANT PROTEIN VARIANTS
    Joanna Floros; Fiscal Year: 2007
    ..Knowledge gained may help explain and perhaps link the in vitro data with the human genetic association data, and provide insight in the understanding of the value of the SP-A gene duplication. ..
  8. SURFACTANT PROTEINS AND RESPIRATORY DISTRESS SYNDROME
    Joanna Floros; Fiscal Year: 2007
    ....
  9. OZONE EFFECTS ON FUNCTION OF SURFACTANT PROTEIN VARIANTS
    Joanna Floros; Fiscal Year: 2007
    ..Knowledge gained may help explain and perhaps link the in vitro data with the human genetic association data, and provide insight in the understanding of the value of the SP-A gene duplication. ..
  10. SURFACTANT PROTEINS AND RESPIRATORY DISTRESS SYNDROME
    Joanna Floros; Fiscal Year: 2010
    ....
  11. The Role of surfactant protein genetic variants in cyst*
    Joanna Floros; Fiscal Year: 2004
    ..aeruginosa and its clearance, and provide the basis for further consideration of novel therapeutic strategies to treat CF-related lung disease. ..
  12. The Role of surfactant protein genetic variants in cyst*
    Joanna Floros; Fiscal Year: 2003
    ..aeruginosa and its clearance, and provide the basis for further consideration of novel therapeutic strategies to treat CF-related lung disease. ..
  13. SURFACTANT PROTEINS AND RESPIRATORY DISTRESS SYNDROME
    Joanna Floros; Fiscal Year: 2002
    ..abstract_text> ..
  14. SURFACTANT PROTEINS AND RESPIRATORY DISTRESS SYNDROME
    Joanna Floros; Fiscal Year: 1999
    ..abstract_text> ..
  15. OZONE EFFECTS ON FUNCTION OF SURFACTANT PROTEIN VARIANTS
    Joanna Floros; Fiscal Year: 2000
    ....
  16. SURFACTANT PROTEINS AND RESPIRATORY DISTRESS SYNDROME
    Joanna Floros; Fiscal Year: 2000
    ..abstract_text> ..
  17. OZONE EFFECTS ON FUNCTION OF SURFACTANT PROTEIN VARIANTS
    Joanna Floros; Fiscal Year: 2001
    ....
  18. SURFACTANT PROTEINS AND RESPIRATORY DISTRESS SYNDROME
    Joanna Floros; Fiscal Year: 2001
    ..abstract_text> ..
  19. The Role of surfactant protein genetic variants in CF
    Joanna Floros; Fiscal Year: 2001
    ..aeruginosa and its clearance, and provide the basis for further consideration of novel therapeutic strategies to treat CF-related lung disease. ..
  20. The Role of surfactant protein genetic variants in cyst*
    Joanna Floros; Fiscal Year: 2002
    ..aeruginosa and its clearance, and provide the basis for further consideration of novel therapeutic strategies to treat CF-related lung disease. ..
  21. SURFACTANT PROTEINS AND RESPIRATORY DISTRESS SYNDROME
    Joanna Floros; Fiscal Year: 1991
    ....
  22. SURFACTANT PROTEINS AND RESPIRATORY DISTRESS SYNDROME
    Joanna Floros; Fiscal Year: 1992
    ....
  23. OZONE EFFECTS ON FUNCTION OF SURFACTANT PROTEIN VARIANTS
    Joanna Floros; Fiscal Year: 1999
    ....