DONALD BRYANT

Summary

Affiliation: Pennsylvania State University
Country: USA

Publications

  1. pmc A new pheromone trail-based genetic algorithm for comparative genome assembly
    Fangqing Zhao
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
    Nucleic Acids Res 36:3455-62. 2008
  2. pmc Synechococcus sp. Strain PCC 7002 Transcriptome: Acclimation to Temperature, Salinity, Oxidative Stress, and Mixotrophic Growth Conditions
    Marcus Ludwig
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University University Park, PA, USA
    Front Microbiol 3:354. 2012
  3. pmc Bacteriochlorophyll f: properties of chlorosomes containing the "forbidden chlorophyll"
    Kajetan Vogl
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park PA, USA
    Front Microbiol 3:298. 2012
  4. pmc Complete Genome of Ignavibacterium album, a Metabolically Versatile, Flagellated, Facultative Anaerobe from the Phylum Chlorobi
    Zhenfeng Liu
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University University Park, PA, USA
    Front Microbiol 3:185. 2012
  5. pmc Acclimation of the Global Transcriptome of the Cyanobacterium Synechococcus sp. Strain PCC 7002 to Nutrient Limitations and Different Nitrogen Sources
    Marcus Ludwig
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University University Park, PA, USA
    Front Microbiol 3:145. 2012
  6. pmc Transcription Profiling of the Model Cyanobacterium Synechococcus sp. Strain PCC 7002 by Next-Gen (SOLiD™) Sequencing of cDNA
    Marcus Ludwig
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University University Park, PA, USA
    Front Microbiol 2:41. 2011
  7. pmc Identification of the bacteriochlorophylls, carotenoids, quinones, lipids, and hopanoids of "Candidatus Chloracidobacterium thermophilum"
    Amaya M Garcia Costas
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, USA
    J Bacteriol 194:1158-68. 2012
  8. ncbi Prokaryotic photosynthesis and phototrophy illuminated
    Donald A Bryant
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
    Trends Microbiol 14:488-96. 2006
  9. pmc Identification of the bchP gene, encoding geranylgeranyl reductase in Chlorobaculum tepidum
    Aline Gomez Maqueo Chew
    Department of Biochemistry and Molecular Biology, S 235 Frear Building, The Pennsylvania State University, University Park, PA 16802, USA
    J Bacteriol 190:747-9. 2008
  10. ncbi Candidatus Chloracidobacterium thermophilum: an aerobic phototrophic Acidobacterium
    Donald A Bryant
    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA
    Science 317:523-6. 2007

Research Grants

Collaborators

Detail Information

Publications78

  1. pmc A new pheromone trail-based genetic algorithm for comparative genome assembly
    Fangqing Zhao
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
    Nucleic Acids Res 36:3455-62. 2008
    ..The software and test data sets can be accessed at http://sourceforge.net/projects/pga4genomics/...
  2. pmc Synechococcus sp. Strain PCC 7002 Transcriptome: Acclimation to Temperature, Salinity, Oxidative Stress, and Mixotrophic Growth Conditions
    Marcus Ludwig
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University University Park, PA, USA
    Front Microbiol 3:354. 2012
    ..However, the transcriptome data suggest that cells must acclimate to excess reducing equivalents when a reduced C-source is present...
  3. pmc Bacteriochlorophyll f: properties of chlorosomes containing the "forbidden chlorophyll"
    Kajetan Vogl
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park PA, USA
    Front Microbiol 3:298. 2012
    ....
  4. pmc Complete Genome of Ignavibacterium album, a Metabolically Versatile, Flagellated, Facultative Anaerobe from the Phylum Chlorobi
    Zhenfeng Liu
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University University Park, PA, USA
    Front Microbiol 3:185. 2012
    ..Comparisons of I. album and other members of the phylum Chlorobi suggest that the physiology of the ancestors of this phylum might have been quite different from that of modern GSB...
  5. pmc Acclimation of the Global Transcriptome of the Cyanobacterium Synechococcus sp. Strain PCC 7002 to Nutrient Limitations and Different Nitrogen Sources
    Marcus Ludwig
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University University Park, PA, USA
    Front Microbiol 3:145. 2012
    ....
  6. pmc Transcription Profiling of the Model Cyanobacterium Synechococcus sp. Strain PCC 7002 by Next-Gen (SOLiD™) Sequencing of cDNA
    Marcus Ludwig
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University University Park, PA, USA
    Front Microbiol 2:41. 2011
    ..Finally, the data suggested that an apparent operon involved in tetrapyrrole biosynthesis and fatty acid desaturation, acsF2-ho2-hemN2-desF, may be regulated by oxygen concentration...
  7. pmc Identification of the bacteriochlorophylls, carotenoids, quinones, lipids, and hopanoids of "Candidatus Chloracidobacterium thermophilum"
    Amaya M Garcia Costas
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, USA
    J Bacteriol 194:1158-68. 2012
    ..Chloracidobacterium thermophilum."..
  8. ncbi Prokaryotic photosynthesis and phototrophy illuminated
    Donald A Bryant
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
    Trends Microbiol 14:488-96. 2006
    ..In this review, we present examples that reflect recent advances in phototroph biology as a result of insights from genome and metagenome sequencing...
  9. pmc Identification of the bchP gene, encoding geranylgeranyl reductase in Chlorobaculum tepidum
    Aline Gomez Maqueo Chew
    Department of Biochemistry and Molecular Biology, S 235 Frear Building, The Pennsylvania State University, University Park, PA 16802, USA
    J Bacteriol 190:747-9. 2008
    ..Each gene was insertionally inactivated, and the resulting mutants were characterized. CT2256 encodes geranylgeranyl reductase (BchP); CT1232 is not involved in bacteriochlorophyll or chlorophyll biosynthesis...
  10. ncbi Candidatus Chloracidobacterium thermophilum: an aerobic phototrophic Acidobacterium
    Donald A Bryant
    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA
    Science 317:523-6. 2007
    ..Candidatus Chloracidobacterium thermophilum" is a BChl-producing member of the poorly characterized phylum Acidobacteria...
  11. ncbi Selective protein extraction from Chlorobium tepidum chlorosomes using detergents. Evidence that CsmA forms multimers and binds bacteriochlorophyll a
    Donald A Bryant
    Department of Biochemistry and Molecular Biology and Center for Biomolecular Structure and Function, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Biochemistry 41:14403-11. 2002
    ..Quantitative estimates suggest that chlorosomes contain approximately equimolar amounts of CsmA and BChl a and that roughly one-third of the surface of the chlorosome is covered by CsmA...
  12. pmc The beauty in small things revealed
    Donald A Bryant
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, S 234 Frear Building, University Park, PA 16802, USA
    Proc Natl Acad Sci U S A 100:9647-9. 2003
  13. pmc Ultrastructural analysis and identification of envelope proteins of "Candidatus Chloracidobacterium thermophilum" chlorosomes
    Amaya M Garcia Costas
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
    J Bacteriol 193:6701-11. 2011
    ..The combined analyses showed that the chlorosomes of "Ca. Chloracidobacterium thermophilum" possess a number of unique features but also share some properties with the chlorosomes found in anaerobic members of other phyla...
  14. ncbi Isolation and characterization of carotenosomes from a bacteriochlorophyll c-less mutant of Chlorobium tepidum
    Niels Ulrik Frigaard
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
    Photosynth Res 86:101-11. 2005
    ..Carotenosomes thus provide a valuable material for studying the biogenesis, structure, and function of the photosynthetic antennae in green bacteria...
  15. pmc Bacteriochlorophyllide c C-8(2) and C-12(1) methyltransferases are essential for adaptation to low light in Chlorobaculum tepidum
    Aline Gomez Maqueo Chew
    Department of Biochemistry and Molecular Biology, S 235 Frear Building, The Pennsylvania State University, University Park, PA 16802, USA
    J Bacteriol 189:6176-84. 2007
    ..tepidum to low light intensity. The data additionally suggest that these methylations also directly or indirectly affect the regulation of the BChl c biosynthetic pathway...
  16. doi Multiple antioxidant proteins protect Chlorobaculum tepidum against oxygen and reactive oxygen species
    Hui Li
    Department of Biochemistry and Molecular Biology and Center for Biomolecular Structure and Function, The Pennsylvania State University, S 235 Frear Building, University Park, PA 16802, USA
    Arch Microbiol 191:853-67. 2009
    ..Reverse-transcriptase polymerase chain reaction studies showed that the genes with functions in oxidative stress protection were constitutively transcribed under anoxic growth conditions...
  17. ncbi Subcellular localization of chlorosome proteins in Chlorobium tepidum and characterization of three new chlorosome proteins: CsmF, CsmH, and CsmX
    Elena V Vassilieva
    Department of Biochemistry and Molecular Biology and Center for Biomolecular Structure and Function, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Biochemistry 41:4358-70. 2002
    ....
  18. pmc Identification of a fourth family of lycopene cyclases in photosynthetic bacteria
    Julia A Maresca
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
    Proc Natl Acad Sci U S A 104:11784-9. 2007
    ..Identification of these cyclases fills a major gap in the carotenoid biosynthetic pathways of green sulfur bacteria and cyanobacteria...
  19. ncbi Chlorophyll biosynthesis in bacteria: the origins of structural and functional diversity
    Aline Gomez Maqueo Chew
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Annu Rev Microbiol 61:113-29. 2007
    ....
  20. pmc Chlorobium tepidum mutant lacking bacteriochlorophyll c made by inactivation of the bchK gene, encoding bacteriochlorophyll c synthase
    Niels Ulrik Frigaard
    Department of Biochemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    J Bacteriol 184:3368-76. 2002
    ....
  21. doi Photosystem I/molecular wire/metal nanoparticle bioconjugates for the photocatalytic production of H2
    Rebecca A Grimme
    Department of Chemistry, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    J Am Chem Soc 130:6308-9. 2008
    ..Supplying the system with more efficient donor-side electron donating species results in a 5-fold increase in the rate of H2 evolution...
  22. ncbi Characterization of two cytochrome oxidase operons in the marine cyanobacterium Synechococcus sp. PCC 7002: inactivation of ctaDI affects the PS I:PS II ratio
    Christopher T Nomura
    Department of Biochemistry and Molecular Biology, Center for Biomolecular Structure and Function, The Pennsylvania State University, University Park, PA 16802, USA
    Photosynth Res 87:215-28. 2006
    ..pcc 7002...
  23. ncbi Seeing green bacteria in a new light: genomics-enabled studies of the photosynthetic apparatus in green sulfur bacteria and filamentous anoxygenic phototrophic bacteria
    Niels Ulrik Frigaard
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16801, USA
    Arch Microbiol 182:265-76. 2004
    ..The Chl. tepidum mutants produced with modified BChl c and carotenoid species also allow the functions of these pigments to be studied in vivo...
  24. pmc Nine mutants of Chlorobium tepidum each unable to synthesize a different chlorosome protein still assemble functional chlorosomes
    Niels Ulrik Frigaard
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    J Bacteriol 186:646-53. 2004
    ..These results demonstrate that chlorosomes are remarkably robust structures that can tolerate considerable changes in protein composition...
  25. ncbi Characterization of the FMO protein from the aerobic chlorophototroph, Candidatus Chloracidobacterium thermophilum
    Yusuke Tsukatani
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
    Photosynth Res 104:201-9. 2010
    ..The results imply that, without changing pigment composition or structure dramatically, the FMO protein has acquired properties that allow it to perform light harvesting efficiently under aerobic conditions...
  26. ncbi Insertional inactivation of the menG gene, encoding 2-phytyl-1,4-naphthoquinone methyltransferase of Synechocystis sp. PCC 6803, results in the incorporation of 2-phytyl-1,4-naphthoquinone into the A(1) site and alteration of the equilibrium constant betw
    Yumiko Sakuragi
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Biochemistry 41:394-405. 2002
    ....
  27. pmc Isorenieratene biosynthesis in green sulfur bacteria requires the cooperative actions of two carotenoid cyclases
    Julia A Maresca
    Department of Biochemistry and Molecular Biology, S 235 Frear Building, The Pennsylvania State University, University Park, PA 16802, USA
    J Bacteriol 190:6384-91. 2008
    ..Consequently, the branch point for the synthesis of mono- and dicyclic carotenoids in GSB seems to be the modification of gamma-carotene, rather than the cyclization of lycopene as occurs in cyanobacteria...
  28. pmc CpcM posttranslationally methylates asparagine-71/72 of phycobiliprotein beta subunits in Synechococcus sp. strain PCC 7002 and Synechocystis sp. strain PCC 6803
    Gaozhong Shen
    Department of Biochemistry and Molecular Biology, S 235 Frear Building, The Pennsylvania State University, University Park, PA 16802, USA
    J Bacteriol 190:4808-17. 2008
    ..The possible functions of asparagine N methylation of phycobiliproteins are discussed...
  29. pmc Genetic manipulation of carotenoid biosynthesis in the green sulfur bacterium Chlorobium tepidum
    Niels Ulrik Frigaard
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
    J Bacteriol 186:5210-20. 2004
    ..The phylogeny of carotenogenic enzymes in green sulfur bacteria and green filamentous bacteria is also discussed...
  30. pmc The Biosynthetic pathway for synechoxanthin, an aromatic carotenoid synthesized by the euryhaline, unicellular cyanobacterium Synechococcus sp. strain PCC 7002
    Joel E Graham
    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA
    J Bacteriol 190:7966-74. 2008
    ..Based on observed and biochemically characterized intermediates, a complete pathway for synechoxanthin biosynthesis is proposed...
  31. doi Biogenesis of phycobiliproteins: I. cpcS-I and cpcU mutants of the cyanobacterium Synechococcus sp. PCC 7002 define a heterodimeric phyococyanobilin lyase specific for beta-phycocyanin and allophycocyanin subunits
    Gaozhong Shen
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    J Biol Chem 283:7503-12. 2008
    ..A., Williams, S. R., Bryant, D. A., and Schluchter, W. M. (2008) J. Biol. Chem. 283, 7513-7522). We conclude that the maturation of PBP in vivo depends on three PCB lyases: CpcE-CpcF, CpcS-I-CpcU, and CpcT...
  32. pmc The biosynthetic pathway for myxol-2' fucoside (myxoxanthophyll) in the cyanobacterium Synechococcus sp. strain PCC 7002
    Joel E Graham
    Department of Biochemistry and Molecular Biology, S 235 Frear Building, The Pennsylvania State University, University Park, PA 16802, USA
    J Bacteriol 191:3292-300. 2009
    ..This study expands the suite of enzymes available for metabolic engineering of carotenoid biosynthetic pathways for biotechnological applications...
  33. ncbi Mutational analysis of three bchH paralogs in (bacterio-)chlorophyll biosynthesis in Chlorobaculum tepidum
    Aline Gomez Maqueo Chew
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, S 235 Frear Building, PA 16802, University Park, USA
    Photosynth Res 101:21-34. 2009
    ....
  34. ncbi Removal of the PsaF polypeptide biases electron transfer in favor of the PsaB branch of cofactors in Triton X-100 photosystem I complexes from Synechococcus sp. PCC 7002
    Rufat Agalarov
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, USA
    Photochem Photobiol 84:1371-80. 2008
    ....
  35. pmc Two genes encoding new carotenoid-modifying enzymes in the green sulfur bacterium Chlorobium tepidum
    Julia A Maresca
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, S 235 Frear Building, PA 16802, USA
    J Bacteriol 188:6217-23. 2006
    ..The other bacteria in which these genes are found are not closely related to green sulfur bacteria or to one another. This suggests that the ability to synthesize modified carotenoids has been a frequently transferred trait...
  36. pmc The bchU gene of Chlorobium tepidum encodes the c-20 methyltransferase in bacteriochlorophyll c biosynthesis
    Julia A Maresca
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
    J Bacteriol 186:2558-66. 2004
    ....
  37. ncbi Molecular contacts for chlorosome envelope proteins revealed by cross-linking studies with chlorosomes from Chlorobium tepidum
    Hui Li
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Biochemistry 45:9095-103. 2006
    ..On the basis of the structural information obtained from these cross-linking experiments, a model for the locations and interactions of the proteins of the chlorosome envelope is proposed...
  38. pmc Biogenesis of iron-sulfur clusters in photosystem I: holo-NfuA from the cyanobacterium Synechococcus sp. PCC 7002 rapidly and efficiently transfers [4Fe-4S] clusters to apo-PsaC in vitro
    Zhao Jin
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    J Biol Chem 283:28426-35. 2008
    ..These results establish that cyanobacterial NfuA can act as a scaffolding protein for the insertion of [4Fe-4S] clusters into PsaC of PS I in vitro...
  39. ncbi Recruitment of a foreign quinone into the A1 site of photosystem I. Characterization of a menB rubA double deletion mutant in Synechococcus sp. PCC 7002 devoid of FX, FA, and FB and containing plastoquinone or exchanged 9,10-anthraquinone
    Yumiko Sakuragi
    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    J Biol Chem 280:12371-81. 2005
    ..The ability to readily displace PQ-9 from the A1 site makes the menB rubA mutant ideal for introducing novel quinones, particularly anthraquinones, into PS I...
  40. pmc Highly divergent methyltransferases catalyze a conserved reaction in tocopherol and plastoquinone synthesis in cyanobacteria and photosynthetic eukaryotes
    Zigang Cheng
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, USA
    Plant Cell 15:2343-56. 2003
    ....
  41. doi Wiring photosystem I for direct solar hydrogen production
    Carolyn E Lubner
    Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Biochemistry 49:404-14. 2010
    ..The controlled light-driven production of strong reductants by such systems can be used to produce other biofuels or to provide mechanistic insights into enzymes catalyzing multielectron, proton-coupled reactions...
  42. doi The biochemical basis for structural diversity in the carotenoids of chlorophototrophic bacteria
    Julia A Maresca
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
    Photosynth Res 97:121-40. 2008
    ....
  43. pmc Regulatory roles for IscA and SufA in iron homeostasis and redox stress responses in the cyanobacterium Synechococcus sp. strain PCC 7002
    Ramakrishnan Balasubramanian
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    J Bacteriol 188:3182-91. 2006
    ....
  44. ncbi Identification and characterization of a new class of bilin lyase: the cpcT gene encodes a bilin lyase responsible for attachment of phycocyanobilin to Cys-153 on the beta-subunit of phycocyanin in Synechococcus sp. PCC 7002
    Gaozhong Shen
    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA
    J Biol Chem 281:17768-78. 2006
    ..The results from characterization of the cpcT mutant as well as the in vitro biochemical assays demonstrate that CpcT is a new phycocyanobilin lyase that specifically attaches phycocyanobilin to Cys-153 of the phycocyanin beta-subunit...
  45. ncbi Group 3 sigma factors in the marine cyanobacterium Synechococcus sp. strain PCC 7002 are required for growth at low temperature
    Kaori Inoue-Sakamoto
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, University Park, PA 16802, USA
    J Gen Appl Microbiol 53:89-104. 2007
    ....
  46. ncbi Characterization of a plant-like protochlorophyllide a divinyl reductase in green sulfur bacteria
    Aline Gomez Maqueo Chew
    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    J Biol Chem 282:2967-75. 2007
    ..Because only some Chl-synthesizing organisms possess homologs of bciA, at least two types of C-8 vinyl reductases must occur...
  47. ncbi Assembly of photosystem I. II. Rubredoxin is required for the in vivo assembly of F(X) in Synechococcus sp. PCC 7002 as shown by optical and EPR spectroscopy
    Gaozhong Shen
    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    J Biol Chem 277:20355-66. 2002
    ..Since the PsaC protein requires the presence of F(X) for binding, the absence of F(A) and F(B) may be an indirect result of the absence of F(X)...
  48. ncbi Assembly of photosystem I. I. Inactivation of the rubA gene encoding a membrane-associated rubredoxin in the cyanobacterium Synechococcus sp. PCC 7002 causes a loss of photosystem I activity
    Gaozhong Shen
    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    J Biol Chem 277:20343-54. 2002
    ..Our results indicate that RubA plays a specific role in the biogenesis of PS I...
  49. pmc The sufR gene (sll0088 in Synechocystis sp. strain PCC 6803) functions as a repressor of the sufBCDS operon in iron-sulfur cluster biogenesis in cyanobacteria
    Tao Wang
    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    J Bacteriol 186:956-67. 2004
    ..We propose that the protein encoded by sll0088 is a transcriptional repressor of the suf operon, and we name the gene sufR...
  50. ncbi Gene inactivation in the cyanobacterium Synechococcus sp. PCC 7002 and the green sulfur bacterium Chlorobium tepidum using in vitro-made DNA constructs and natural transformation
    Niels Ulrik Frigaard
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, USA
    Methods Mol Biol 274:325-40. 2004
    ..These methods are ligation of DNA fragments with T4 DNA ligase, and megaprimer PCR...
  51. pmc Envelope proteins of the CsmB/CsmF and CsmC/CsmD motif families influence the size, shape, and composition of chlorosomes in Chlorobaculum tepidum
    Hui Li
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
    J Bacteriol 191:7109-20. 2009
    ....
  52. ncbi Truncated hemoglobin from the cyanobacterium Synechococcus sp. PCC 7002: evidence for hexacoordination and covalent adduct formation in the ferric recombinant protein
    Nancy L Scott
    Department of Chemistry and Center for Biomolecular Structure and Function, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Biochemistry 41:6902-10. 2002
    ..Both hemoglobins were found capable of forming a product in which the heme is cross-linked to the polypeptide through modification of a vinyl group...
  53. doi Wiring an [FeFe]-hydrogenase with photosystem I for light-induced hydrogen production
    Carolyn E Lubner
    Department of Chemistry, The Pennsylvania State University, University Park, PA 16802, USA
    Biochemistry 49:10264-6. 2010
    ..These hydrogen-producing nanoconstructs self-assemble when the PS I variant, the H(2)ase variant, and the molecular wire are combined...
  54. pmc Multiple types of 8-vinyl reductases for (bacterio)chlorophyll biosynthesis occur in many green sulfur bacteria
    Zhenfeng Liu
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    J Bacteriol 193:4996-8. 2011
    ..Pigment analyses of the complemented strains showed that the bciB homologs encode 8-vinyl reductases similar to those of cyanobacteria...
  55. pmc Effects of modified Phycobilin biosynthesis in the Cyanobacterium Synechococcus sp. Strain PCC 7002
    Richard M Alvey
    Department of Biochemistry and Molecular Biology, S 235 Frear Building, The Pennsylvania State University, University Park, PA 16802, USA
    J Bacteriol 193:1663-71. 2011
    ..As a result, the strain producing phycobiliproteins carrying phytochromobilin grew much more slowly at low light intensity...
  56. doi Roles of xanthophyll carotenoids in protection against photoinhibition and oxidative stress in the cyanobacterium Synechococcus sp. strain PCC 7002
    Yuehui Zhu
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
    Arch Biochem Biophys 504:86-99. 2010
    ..These studies indicated that all xanthophylls contribute to protection against photo-oxidative stress...
  57. doi Synechoxanthin, an aromatic C40 xanthophyll that is a major carotenoid in the cyanobacterium Synechococcus sp. PCC 7002
    Joel E Graham
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    J Nat Prod 71:1647-50. 2008
    ..This discovery conclusively establishes that some cyanobacteria are capable of synthesizing aromatic carotenoids...
  58. doi Functional and structural characterization of the 2/2 hemoglobin from Synechococcus sp. PCC 7002
    Nancy L Scott
    Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Biochemistry 49:7000-11. 2010
    ..The solution structure of covalently modified GlbN was determined and used to rationalize some of its chemical properties...
  59. pmc alpha-Tocopherol plays a role in photosynthesis and macronutrient homeostasis of the cyanobacterium Synechocystis sp. PCC 6803 that is independent of its antioxidant function
    Yumiko Sakuragi
    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, 16802, USA
    Plant Physiol 141:508-21. 2006
    ..Based on these results, we propose that alpha-tocopherol plays a nonantioxidant regulatory role in photosynthesis and macronutrient homeostasis through a signal transduction pathway that also involves PmgA...
  60. ncbi Chemical rescue of a site-modified ligand to a [4Fe-4S] cluster in PsaC, a bacterial-like dicluster ferredoxin bound to Photosystem I
    Mikhail L Antonkine
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
    Biochim Biophys Acta 1767:712-24. 2007
    ..In particular, it provides a mechanism to attach an additional electron transfer cofactor to the protein via a bound, external ligand...
  61. pmc Tocopherols protect Synechocystis sp. strain PCC 6803 from lipid peroxidation
    Hiroshi Maeda
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, USA
    Plant Physiol 138:1422-35. 2005
    ..strain PCC 6803 from lipid peroxidation and HL stress...
  62. pmc The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium
    Jonathan A Eisen
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Proc Natl Acad Sci U S A 99:9509-14. 2002
    ..tepidum and many Archaeal species...
  63. ncbi Interaction of ferredoxin:NADP+ oxidoreductase with phycobilisomes and phycobilisome substructures of the cyanobacterium Synechococcus sp. strain PCC 7002
    Carlos Gómez-Lojero
    Departamento de Bioquimica, Centro de Investigacion y Estudios Avanzados del IPN, Apartado Postal 14 740, 07000, Mexico D F, Mexico
    Biochemistry 42:13800-11. 2003
    ..The implications of this localization of FNR within the PBS with respect to its function in cyanobacteria are discussed...
  64. ncbi Roles for heme-copper oxidases in extreme high-light and oxidative stress response in the cyanobacterium Synechococcus sp. PCC 7002
    Christopher T Nomura
    Department of Chemistry, State University of New York, College of Environmental Science and Forestry, 1 Forestry Drive, Syracuse, NY, 13210 2726, USA
    Arch Microbiol 185:471-9. 2006
    ..These results suggest that the CtaII cytochrome oxidase may be involved with the oxidative stress response, including the control of SOD expression...
  65. ncbi Solution structure of the unbound, oxidized Photosystem I subunit PsaC, containing [4Fe-4S] clusters F(A) and F(B): a conformational change occurs upon binding to photosystem I
    Mikhail L Antonkine
    Present address Institut für Experimentalphysik, Freie Universitat Berlin, Arnimallee 14, 14195 Berlin, Germany
    J Biol Inorg Chem 7:461-72. 2002
    ..Electronic supplementary material to this paper can be obtained by using the Springer Link server located at http://dx.doi.org/10.1007/s00775-001-0321-3...
  66. pmc Transcriptional regulation of the CO2-concentrating mechanism in a euryhaline, coastal marine cyanobacterium, Synechococcus sp. Strain PCC 7002: role of NdhR/CcmR
    Fiona J Woodger
    Molecular Plant Physiology Group, Research School of Biological Sciences, Australian National University, P O Box 475, Canberra, ACT 0200, Australia
    J Bacteriol 189:3335-47. 2007
    ..We propose that CcmR (and its associated regulon) represents a specialization for species inhabiting environments subject to fluctuating C(i) concentrations...
  67. pmc Triplet exciton formation as a novel photoprotection mechanism in chlorosomes of Chlorobium tepidum
    Hanyoup Kim
    Department of Physics, Purdue University, West Lafayette, Indiana, USA
    Biophys J 93:192-201. 2007
    ..Thus, the formation of triplet excitons in chlorosomes serves as an alternative photoprotection mechanism...
  68. pmc Unexpected diversity and complexity of the Guerrero Negro hypersaline microbial mat
    Ruth E Ley
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Appl Environ Microbiol 72:3685-95. 2006
    ..The sequences determined in this study have been submitted to the GenBank database and assigned accession numbers DQ 329539 to DQ 331020, and DQ 397339 to DQ 397511...
  69. pmc Suppressor mutations in the study of photosystem I biogenesis: sll0088 is a previously unidentified gene involved in reaction center accumulation in Synechocystis sp. strain PCC 6803
    Jianping Yu
    MSU DOE Plant Research Laboratory and Biochemistry and Molecular Biology Department, Michigan State University, East Lansing, Michigan 48824, USA
    J Bacteriol 185:3878-87. 2003
    ..The protein contains a typical helix-turn-helix DNA-binding motif and can be classified as a negative regulator by phylogenetic analysis. This suggests that the product of sll0088 has a role in regulating the biogenesis of photosystem I...
  70. ncbi Removal of PsaF alters forward electron transfer in photosystem I: evidence for fast reoxidation of QK-A in subunit deletion mutants of Synechococcus sp. PCC 7002
    Art van der Est
    Department of Chemistry, Brock University, 500 Glenridge Avenue, St Catharines, Ontario, Canada L2S 3A1
    Biochemistry 43:1264-75. 2004
    ....
  71. ncbi The secondary electron acceptor of photosystem I in Gloeobacter violaceus PCC 7421 is menaquinone-4 that is synthesized by a unique but unknown pathway
    Mamoru Mimuro
    Department of Technology and Ecology, Hall of Global Environmental Research, Kyoto University, Kyoto 606 8501, Japan
    FEBS Lett 579:3493-6. 2005
    ..violaceus genome. Since G. violaceus clearly synthesizes MQ-4, the combined results indicate that this cyanobacterium must have a novel pathway for the synthesis of 1,4-dihydroxy-2-naphthoic acid...
  72. ncbi Long-range organization of bacteriochlorophyll in chlorosomes of Chlorobium tepidum investigated by cryo-electron microscopy
    Gert T Oostergetel
    Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    FEBS Lett 581:5435-9. 2007
    ..tepidum chlorosomes do not have a highly regular, long-range BChl c layer organization and that they contain several multi-layered tubules rather than single-layer tubules or exclusively undulating lamellae as previously proposed...
  73. doi Biogenesis of phycobiliproteins. III. CpcM is the asparagine methyltransferase for phycobiliprotein beta-subunits in cyanobacteria
    Crystal A Miller
    Department of Biological Sciences, University of New Orleans, New Orleans, Louisiana 70148, USA
    J Biol Chem 283:19293-300. 2008
    ..Based upon these in vitro studies, we conclude that this post-translational modification probably occurs after chromophorylation but before trimer assembly in vivo...
  74. ncbi Kinetic analyses of state transitions of the cyanobacterium Synechococcus sp. PCC 7002 and its mutant strains impaired in electron transport
    Chenhui Huang
    State Key Labaratory of Protein and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China
    Biochim Biophys Acta 1607:121-30. 2003
    ..The possible mechanism for sensing an imbalanced light regime in cyanobacterial state transitions is discussed...
  75. ncbi UreG, a chaperone in the urease assembly process, is an intrinsically unstructured GTPase that specifically binds Zn2+
    Barbara Zambelli
    Laboratory of Bioinorganic Chemistry, Department of Agro Environmental Science and Technology, University of Bologna, I 40127 Bologna, Italy
    J Biol Chem 280:4684-95. 2005
    ..It is also proposed that metal ions such as Zn2+ could have important structural roles in the urease activation process...
  76. pmc Biogenesis of phycobiliproteins: II. CpcS-I and CpcU comprise the heterodimeric bilin lyase that attaches phycocyanobilin to CYS-82 OF beta-phycocyanin and CYS-81 of allophycocyanin subunits in Synechococcus sp. PCC 7002
    Nicolle A Saunee
    Department of Biological Sciences, University of New Orleans, New Orleans, Louisiana 70148, USA
    J Biol Chem 283:7513-22. 2008
    ..Therefore, CpcSU is the bilin lyase-responsible for attachment of PCB to Cys-82 of CpcB and Cys-81 of ApcA and ApcB...
  77. ncbi Comparative genomics provides evidence for the 3-hydroxypropionate autotrophic pathway in filamentous anoxygenic phototrophic bacteria and in hot spring microbial mats
    Christian G Klatt
    Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT, USA
    Environ Microbiol 9:2067-78. 2007
    ..were participating in photoheterotrophic uptake of cyanobacterial photosynthate produced by the reductive pentose phosphate cycle...
  78. ncbi Molecular characterization of Bacillus pasteurii UreE, a metal-binding chaperone for the assembly of the urease active site
    Stefano Ciurli
    Department of Agro Environmental Science and Technology, University of Bologna, Via Filippo Re 8, Italy
    J Biol Inorg Chem 7:623-31. 2002
    ..Several secondary structure elements, including a betaalphabetabetaalphabeta "ferredoxin-like" motif, are highly conserved throughout the UreE sequences...

Research Grants20

  1. REGULATION OF BILIPROTEIN BIOSYNTHESIS IN CYANOBACTERIA
    DONALD BRYANT; Fiscal Year: 1990
    ..PCC 7409 in an attempt to generate at in vitro system for the analysis of transcriptional regulation in cyanobacteria. The studies proposed should greatly extend out knowledge of mechanisms regulating gene expression in photoautotrophs...
  2. REGULATION OF BILIPROTEIN BIOSYNTHESIS IN CYANOBACTERIA
    DONALD BRYANT; Fiscal Year: 1991
    ..PCC 7409 in an attempt to generate at in vitro system for the analysis of transcriptional regulation in cyanobacteria. The studies proposed should greatly extend out knowledge of mechanisms regulating gene expression in photoautotrophs...
  3. REGULATION OF BILIPROTEIN BIOSYNTHESIS IN CYANOBACTERIA
    DONALD BRYANT; Fiscal Year: 1992
    ..PCC 7409 in an attempt to generate at in vitro system for the analysis of transcriptional regulation in cyanobacteria. The studies proposed should greatly extend out knowledge of mechanisms regulating gene expression in photoautotrophs...
  4. REGULATION OF BILIPROTEIN BIOSYNTHESIS IN CYANOBACTERIA
    DONALD BRYANT; Fiscal Year: 1993
    ..PCC 7409 in an attempt to generate at in vitro system for the analysis of transcriptional regulation in cyanobacteria. The studies proposed should greatly extend out knowledge of mechanisms regulating gene expression in photoautotrophs...
  5. REGULATION OF BILIPROTEIN IN CYANOBACTERIA
    DONALD BRYANT; Fiscal Year: 2000
    ..These last studies could explain bloom disappearance in natural populations and could suggest new approaches for the control of problematic or toxic cyanobacteria in water supplies. ..
  6. REGULATION OF BILIPROTEIN IN CYANOBACTERIA
    DONALD BRYANT; Fiscal Year: 2001
    ..These last studies could explain bloom disappearance in natural populations and could suggest new approaches for the control of problematic or toxic cyanobacteria in water supplies. ..
  7. REGULATION OF BILIPROTEIN IN CYANOBACTERIA
    DONALD BRYANT; Fiscal Year: 1999
    ..These last studies could explain bloom disappearance in natural populations and could suggest new approaches for the control of problematic or toxic cyanobacteria in water supplies. ..
  8. REGULATION OF BILIPROTEIN IN CYANOBACTERIA
    DONALD BRYANT; Fiscal Year: 2002
    ..These last studies could explain bloom disappearance in natural populations and could suggest new approaches for the control of problematic or toxic cyanobacteria in water supplies. ..