Stephen J Benkovic


Affiliation: Pennsylvania State University
Country: USA


  1. Guo F, Li P, French J, Mao Z, Zhao H, Li S, et al. Controlling cell-cell interactions using surface acoustic waves. Proc Natl Acad Sci U S A. 2015;112:43-8 pubmed publisher
  2. Talukder P, Chen S, Liu C, Baldwin E, Benkovic S, Hecht S. Tryptophan-based fluorophores for studying protein conformational changes. Bioorg Med Chem. 2014;22:5924-34 pubmed publisher
  3. Zhao H, Chiaro C, Zhang L, Smith P, Chan C, Pedley A, et al. Quantitative analysis of purine nucleotides indicates that purinosomes increase de novo purine biosynthesis. J Biol Chem. 2015;290:6705-13 pubmed publisher
    ..Collectively, these results provide further evidence that purinosome assembly is directly related to activated de novo purine biosynthesis, consistent with the functionality of the purinosome. ..
  4. Chan C, Zhao H, Pugh R, Pedley A, French J, Jones S, et al. Purinosome formation as a function of the cell cycle. Proc Natl Acad Sci U S A. 2015;112:1368-73 pubmed publisher
    ..Our results demonstrate that purinosome formation is closely related to the cell cycle. ..
  5. Pedley A, Benkovic S. Detecting Purinosome Metabolon Formation with Fluorescence Microscopy. Methods Mol Biol. 2018;1764:279-289 pubmed publisher
    ..These protocols define a purinosome as a colocalization of FGAMS with one additional pathway enzyme, such as PPAT or GART, and provide insights into the proper identification of a purinosome from other reported cellular bodies. ..
  6. Noble E, Spiering M, Benkovic S. Coordinated DNA Replication by the Bacteriophage T4 Replisome. Viruses. 2015;7:3186-200 pubmed publisher
    ..Future studies will likely focus on defining the orientations and architecture of protein complexes at the replication fork. ..
  7. Benkovic S, Theriot J, Ringe D. Open questions - in brief: beyond -omics, missing motor proteins, and getting from molecules to organisms. BMC Biol. 2013;11:8 pubmed publisher
  8. Hedglin M, Pandey B, Benkovic S. Stability of the human polymerase δ holoenzyme and its implications in lagging strand DNA synthesis. Proc Natl Acad Sci U S A. 2016;113:E1777-86 pubmed publisher
    ..Such behavior has profound implications on Okazaki fragment synthesis in humans as it limits the processivity of pol δ on undamaged DNA and promotes the rapid dissociation of pol δ from PCNA on stalling at a DNA lesion. ..
  9. Pedley A, Benkovic S. A New View into the Regulation of Purine Metabolism: The Purinosome. Trends Biochem Sci. 2017;42:141-154 pubmed publisher

More Information


  1. request reprint
    Benkovic S, Baker S, Alley M, Woo Y, Zhang Y, Akama T, et al. Identification of borinic esters as inhibitors of bacterial cell growth and bacterial methyltransferases, CcrM and MenH. J Med Chem. 2005;48:7468-76 pubmed
    ..Our data demonstrate the potential for further development of borinic esters as antibacterial agents as well as leads to explore more specific inhibitors against two essential bacterial enzymes...
  2. Spiering M, Hanoian P, Gannavaram S, Benkovic S. RNA primer-primase complexes serve as the signal for polymerase recycling and Okazaki fragment initiation in T4 phage DNA replication. Proc Natl Acad Sci U S A. 2017;114:5635-5640 pubmed publisher
    ..The results reproduce experimental data, providing insights into events related to Okazaki fragment initiation and the overall functioning of DNA replisomes. ..
  3. Benkovic S, Spiering M. Understanding DNA replication by the bacteriophage T4 replisome. J Biol Chem. 2017;292:18434-18442 pubmed publisher
    ..We conclude with a brief comparison with other replisomes with emphasis on how coordinated DNA replication is achieved. ..