K K Wang

Summary

Affiliation: Parke-Davis Pharmaceutical Research
Country: USA

Publications

  1. ncbi request reprint Simultaneous degradation of alphaII- and betaII-spectrin by caspase 3 (CPP32) in apoptotic cells
    K K Wang
    Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48106, USA
    J Biol Chem 273:22490-7. 1998
  2. ncbi request reprint Caspase-mediated fragmentation of calpain inhibitor protein calpastatin during apoptosis
    K K Wang
    Department of Neuroscience Therapeutics, Division of Warner Lambert Company, Parke Davis Pharmaceutical Research, 2800 Plymouth Road, Ann Arbor, Michigan, 48105, USA
    Arch Biochem Biophys 356:187-96. 1998
  3. ncbi request reprint A purine nucleoside phosphorylase (PNP) inhibitor induces apoptosis via caspase-3-like protease activity in MOLT-4 T cells
    R Posmantur
    Parke Davis Pharmaceutical Research, Warner Lambert Company, Department of Immunopathology, Ann Arbor, MI 48105, USA
    Immunopharmacology 37:231-44. 1997
  4. ncbi request reprint Caspase-mediated calcineurin activation contributes to IL-2 release during T cell activation
    N Mukerjee
    Laboratory of Neuro biochemistry, Department of CNS Molecular Sciences, Pfizer Global Research and Development, Ann Arbor Laboratories, 2800 Plymouth Road, Ann Arbor, Michigan 48105, USA
    Biochem Biophys Res Commun 285:1192-9. 2001
  5. ncbi request reprint Thapsigargin induces apoptosis in SH-SY5Y neuroblastoma cells and cerebrocortical cultures
    R Nath
    Department of Neuroscience Therapeutics, Parke Davis Pharmaceutical Research, Ann Arbor, Michigan 48105, USA
    Biochem Mol Biol Int 43:197-205. 1997
  6. ncbi request reprint Calcium/calmodulin-dependent protein kinase inhibition potentiates thapsigargin-mediated cell death in SH-SY5Y human neuroblastoma cells
    K M McGinnis
    Department of Pharmacology, The University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Neurosci Lett 301:99-102. 2001
  7. ncbi request reprint Activation of apoptosis-linked caspase(s) in NMDA-injured brains in neonatal rats
    R Nath
    Department of Neuroscience Therapeutics, Parke Davis Pharmaceutical Research, Division of Warner Lambert Company, Ann Arbor, Michigan, 48105, USA
    Neurochem Int 36:119-26. 2000
  8. ncbi request reprint Inhibition of p38 kinase mimics survival signal-linked protection against apoptosis in rat cerebellar granule neurons
    R Nath
    Dept of Neuroscience Therapeutics, Pfizer Global Research and Development, Ann Arbor, MI 48105, USA
    Cell Mol Biol Lett 6:173-84. 2001
  9. ncbi request reprint Total protein extraction from cultured cells for use in electrophoresis and western blotting
    K K Wang
    Department of Neuroscience Therapeutics, Parke Davis Pharmaceutical Research, Division of Warner Lambert Company, Ann Arbor, MI 48105, USA
    Biotechniques 20:662-8. 1996
  10. ncbi request reprint Molecular cloning and characterization of a novel caspase-3 variant that attenuates apoptosis induced by proteasome inhibition
    Y Huang
    Department of Neuroscience Therapeutics, Pfizer Global Research and Development, Ann Arbor Laboratories, 2800 Plymouth Road, Ann Arbor, Michigan 48105, USA
    Biochem Biophys Res Commun 283:762-9. 2001

Collaborators

Detail Information

Publications24

  1. ncbi request reprint Simultaneous degradation of alphaII- and betaII-spectrin by caspase 3 (CPP32) in apoptotic cells
    K K Wang
    Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48106, USA
    J Biol Chem 273:22490-7. 1998
    ..We postulate that in concert with calpain, caspase rapidly targets critical sites in both alphaII- and betaII-spectrin and thereby initiates a rapid dissolution of the spectrin-actin cortical cytoskeleton with apoptosis...
  2. ncbi request reprint Caspase-mediated fragmentation of calpain inhibitor protein calpastatin during apoptosis
    K K Wang
    Department of Neuroscience Therapeutics, Division of Warner Lambert Company, Parke Davis Pharmaceutical Research, 2800 Plymouth Road, Ann Arbor, Michigan, 48105, USA
    Arch Biochem Biophys 356:187-96. 1998
    ..We propose that the proteolysis of calpastatin in early apoptosis might have yet unidentified effects on the cross-talk between the two protease systems...
  3. ncbi request reprint A purine nucleoside phosphorylase (PNP) inhibitor induces apoptosis via caspase-3-like protease activity in MOLT-4 T cells
    R Posmantur
    Parke Davis Pharmaceutical Research, Warner Lambert Company, Department of Immunopathology, Ann Arbor, MI 48105, USA
    Immunopharmacology 37:231-44. 1997
    ..In summary, we provided several lines of evidence for the role of apoptosis and the contribution of caspase-3-like proteases in T-cell death following PNP inhibition...
  4. ncbi request reprint Caspase-mediated calcineurin activation contributes to IL-2 release during T cell activation
    N Mukerjee
    Laboratory of Neuro biochemistry, Department of CNS Molecular Sciences, Pfizer Global Research and Development, Ann Arbor Laboratories, 2800 Plymouth Road, Ann Arbor, Michigan 48105, USA
    Biochem Biophys Res Commun 285:1192-9. 2001
    ..Taken together, our results suggest that caspase-mediated cleavage of Cn A contributes to IL-2 production during T cell activation...
  5. ncbi request reprint Thapsigargin induces apoptosis in SH-SY5Y neuroblastoma cells and cerebrocortical cultures
    R Nath
    Department of Neuroscience Therapeutics, Parke Davis Pharmaceutical Research, Ann Arbor, Michigan 48105, USA
    Biochem Mol Biol Int 43:197-205. 1997
    ..We propose that thapsigargin-induced cell death in cultured neuronal cells may be a useful system to study the molecular and genetic events involved in apoptosis...
  6. ncbi request reprint Calcium/calmodulin-dependent protein kinase inhibition potentiates thapsigargin-mediated cell death in SH-SY5Y human neuroblastoma cells
    K M McGinnis
    Department of Pharmacology, The University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Neurosci Lett 301:99-102. 2001
    ..Furthermore, thapsigargin-induced caspase-3-like activation, demonstrated by poly(ADP)ribose polymerase cleavage and pro-caspase-3 processing, was elevated in the presence of KN93...
  7. ncbi request reprint Activation of apoptosis-linked caspase(s) in NMDA-injured brains in neonatal rats
    R Nath
    Department of Neuroscience Therapeutics, Parke Davis Pharmaceutical Research, Division of Warner Lambert Company, Ann Arbor, Michigan, 48105, USA
    Neurochem Int 36:119-26. 2000
    ..Lastly, we note that i.p. injection (100 mg/kg) of a pan caspase inhibitor Z-D-DCB attenuates the levels of SBDP120. Our results suggest the presence of caspase-activation in this rat pup model of NMDA toxicity...
  8. ncbi request reprint Inhibition of p38 kinase mimics survival signal-linked protection against apoptosis in rat cerebellar granule neurons
    R Nath
    Dept of Neuroscience Therapeutics, Pfizer Global Research and Development, Ann Arbor, MI 48105, USA
    Cell Mol Biol Lett 6:173-84. 2001
    ..The data provides evidence for an essential role of p38 kinase in mediating apoptotic cell death in CGN and the inhibition of p38 kinase mimics the suppression of apoptosis provided by natural survival signals...
  9. ncbi request reprint Total protein extraction from cultured cells for use in electrophoresis and western blotting
    K K Wang
    Department of Neuroscience Therapeutics, Parke Davis Pharmaceutical Research, Division of Warner Lambert Company, Ann Arbor, MI 48105, USA
    Biotechniques 20:662-8. 1996
    ....
  10. ncbi request reprint Molecular cloning and characterization of a novel caspase-3 variant that attenuates apoptosis induced by proteasome inhibition
    Y Huang
    Department of Neuroscience Therapeutics, Pfizer Global Research and Development, Ann Arbor Laboratories, 2800 Plymouth Road, Ann Arbor, Michigan 48105, USA
    Biochem Biophys Res Commun 283:762-9. 2001
    ..Overexpression of caspase-3s in 293 cells is more resistant to apoptosis induced by proteasome inhibition. Furthermore, we identified that proteasome inhibition stabilized the level of caspase-3s...
  11. ncbi request reprint Evaluation of HIV-1 Tat induced neurotoxicity in rat cortical cell culture
    A Perez
    Department of Infectious Diseases, Parke Davis Pharmaceutical Research, Ann Arbor, MI 48105, USA
    J Neurovirol 7:1-10. 2001
    ..Results presented in this paper suggest that activation of NMDA receptors by HIV-1 Tat is responsible for neuronal cell death in primary rat cortical neurons...
  12. ncbi request reprint Calpain and caspase: can you tell the difference?
    K K Wang
    Dept of Neuroscience Therapeutics, Parke Davis Pharmaceutical Research, Warner Lambert Company, Ann Arbor, MI 48105, USA
    Trends Neurosci 23:20-6. 2000
    ..Caspase inhibitors strongly suppress apoptotic neuronal death. Thus, both protease families might contribute to structural derangement and functional loss in neurons under degenerative conditions...
  13. ncbi request reprint Processing of cdk5 activator p35 to its truncated form (p25) by calpain in acutely injured neuronal cells
    R Nath
    Department of Neuroscience Therapeutics, Parke Davis Pharmaceutical Research, Division of Warner Lambert Company, 2800 Plymouth Road, Ann Arbor, Michigan 48105, USA
    Biochem Biophys Res Commun 274:16-21. 2000
    ..These data suggest that calpain is a major neuronal protease capable of converting p35 to p25 and might play a pathological role of activating cdk5 and its phosphorylation of tau in Alzheimer's disease...
  14. ncbi request reprint Calcium/calmodulin-dependent protein kinase IV is cleaved by caspase-3 and calpain in SH-SY5Y human neuroblastoma cells undergoing apoptosis
    K M McGinnis
    Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    J Biol Chem 273:19993-20000. 1998
    ..Furthermore, calmodulin-stimulated protein kinase activity decreases within 6 h in thapsigargin-treated SH-SY5Y. The loss of activity precedes cell death...
  15. ncbi request reprint The calpain family and human disease
    Y Huang
    Laboratory of Neuro biochemistry, Dept of CNS Molecular Sciences, Pfizer Global Research and Development, 2800 Plymouth Road, Ann Arbor, MI 48105, USA
    Trends Mol Med 7:355-62. 2001
    ..This review describes our current understanding of the calpain family members and their mechanistic linkages to the aforementioned diseases as well as other emerging pathological conditions...
  16. ncbi request reprint TNF-alpha stimulates caspase-3 activation and apoptotic cell death in primary septo-hippocampal cultures
    X Zhao
    The Vivian L. Smith Center for Neurologic Research, Department of Neurosurgery, The University of Texas Health Science Center, Houston, Texas, USA
    J Neurosci Res 64:121-31. 2001
    ..Results of this study suggest TNF-alpha may induce caspase-3 activation but not calpain activation in septo-hippocampal cultures and that this activation of caspase-3 at least partially contributes to TNF-alpha-induced apoptosis...
  17. ncbi request reprint Crystal structure of calcium bound domain VI of calpain at 1.9 A resolution and its role in enzyme assembly, regulation, and inhibitor binding
    G D Lin
    Center for Macromolecular Crystallography, University of Alabama at Birmingham 35294, USA
    Nat Struct Biol 4:539-47. 1997
    ..The crystal structure of a Ca2+ bound domain VI-inhibitor (PD150606) complex has been refined to 2.1 A resolution. A possible mode for calpain inhibition is discussed...
  18. ncbi request reprint Temporal and spatial profile of caspase 8 expression and proteolysis after experimental traumatic brain injury
    R Beer
    Department of Neurology, University Hospital Innsbruck, Austria
    J Neurochem 78:862-73. 2001
    ..Our findings also suggest a contributory role of caspase 8 activation to caspase 3 mediated apoptotic cell death after experimental TBI in vivo...
  19. ncbi request reprint Concurrent assessment of calpain and caspase-3 activation after oxygen-glucose deprivation in primary septo-hippocampal cultures
    J K Newcomb-Fernandez
    Department of Neurosurgery, The Vivian L. Smith Center for Neurologic Research, University of Texas Health Science Center, Houston, Texas, USA
    J Cereb Blood Flow Metab 21:1281-94. 2001
    ....
  20. ncbi request reprint The calpain small subunit gene is essential: its inactivation results in embryonic lethality
    U J Zimmerman
    Institute for Environmental Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104 6068, USA
    IUBMB Life 50:63-8. 2000
    ..The results indicate that at least one normal allele is essential for postnatal survival. Disruption of both alleles appears to be lethal in very early fetal development...
  21. ncbi request reprint Protein kinase C phosphorylates the carboxyl terminus of the plasma membrane Ca(2+)-ATPase from human erythrocytes
    K K Wang
    Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, Canada
    J Biol Chem 266:9078-85. 1991
    ....
  22. ncbi request reprint Differential expression of a cDNA clone in human liver versus hepatic cancer--highly homologous to aryl-dialkyl-phosphatase
    K K Wang
    National Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute
    Cell Res 7:79-90. 1997
    ..The biological significance of its differential expression in normal versus malignant tissue is discussed...
  23. ncbi request reprint Phosphorylation of connexin-32 by protein kinase C prevents its proteolysis by mu-calpain and m-calpain
    M Elvira
    Instituto de Investigaciones Biomedicas, Consejo Superior de Investigaciones Cientificas, Madrid, Spain
    J Biol Chem 268:14294-300. 1993
    ..Phosphorylation of connexin-32 by protein kinase A and protein kinase C does not prevent its proteolysis by papain, alpha-chymotrypsin, proteinase K, and trypsin...
  24. ncbi request reprint The novel calpain inhibitor SJA6017 improves functional outcome after delayed administration in a mouse model of diffuse brain injury
    N C Kupina
    Neuroscience Therapeutics, Pfizer Global Research and Development-Ann Arbor Laboratories, Michigan 48015, USA
    J Neurotrauma 18:1229-40. 2001
    ..However, measurements of regional spectrin degradation may not be the most sensitive marker for determining the effects of calpain inhibition...