Research Topics
Genomes and Genes
| William M PandakSummaryCountry: USA Publications
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Publications
Regulation of oxysterol 7alpha-hydroxylase (CYP7B1) in primary cultures of rat hepatocytesWilliam M Pandak
Department of Medicine, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA 23249, USA
Hepatology 35:1400-8. 2002..In conclusion, in the rat, CYP7B1 specific activity is highly regulated but does not seem to be rate limiting for bile acid synthesis...- Localization of StarD5 cholesterol binding proteinDaniel Rodriguez-Agudo
Department of Medicine, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA, USA
J Lipid Res 47:1168-75. 2006..The presence of this cholesterol/25-hydroxycholesterol-binding protein in cells related to inflammatory processes provides new clues to the role of this protein in free sterol transport in the cells and in lipid-mediated atherogenesis... - Overexpression of cholesterol transporter StAR increases in vivo rates of bile acid synthesis in the rat and mouseShunlin Ren
Department of Medicine, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA, USA
Hepatology 40:910-7. 2004..01). In conclusion, in both rats and mice in vivo, overexpression of StAR led to a marked increase in the rates of BAS initiated by delivery of cholesterol to mitochondria containing CYP27A1... 
Subcellular localization and regulation of StarD4 protein in macrophages and fibroblastsDaniel Rodriguez-Agudo
Department of Medicine, Virginia Commonwealth University, Richmond, VA, USA
Biochim Biophys Acta 1811:597-606. 2011....- Cholesterol metabolite, 5-cholesten-3β-25-diol-3-sulfate, promotes hepatic proliferation in miceXin Zhang
Department of Medicine, Virginia Commonwealth University Veterans Affairs McGuire Medical Center, 1201 Broad Rock Boulevard, Richmond, VA 23249, United States
J Steroid Biochem Mol Biol 132:262-70. 2012..Conclusions: 25HC3S may be a potent regulator of hepatocyte proliferation and oxysterol sulfation may represent a novel regulatory pathway in liver proliferation via inactivating LXR signaling... - Effect of increasing the expression of cholesterol transporters (StAR, MLN64, and SCP-2) on bile acid synthesisShunlin Ren
Department of Medicine, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA, USA
J Lipid Res 45:2123-31. 2004..These results suggest that MLN64, in its full-length form, is not responsible for the transport of cholesterol to the mitochondria or the endoplasmic reticulum, where CYP27A1 or CYP7A1 is located, respectively... - Bile acids regulate hepatic gluconeogenic genes and farnesoid X receptor via G(alpha)i-protein-coupled receptors and the AKT pathwayRisheng Cao
Departments of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23298, USA
J Lipid Res 51:2234-44. 2010..Activation of the insulin signaling pathway appears to be linked to the upregulation of farnesoid X receptor functional activity and SHP induction... - Sulfation of 25-hydroxycholesterol by SULT2B1b decreases cellular lipids via the LXR/SREBP-1c signaling pathway in human aortic endothelial cellsQianming Bai
Department of Medicine, Virginia Commonwealth University Veterans Affairs McGuire Medical Center, Richmond, VA 23249, United States
Atherosclerosis 214:350-6. 2011..We evaluate the effect of 25HC sulfation on lipid metabolism by overexpressing the gene encoding SULT2B1b in human aortic endothelial cells (HAECs) in culture... - Oxysterol sulfation by cytosolic sulfotransferase suppresses liver X receptor/sterol regulatory element binding protein-1c signaling pathway and reduces serum and hepatic lipids in mouse models of nonalcoholic fatty liver diseaseQianming Bai
Department of Medicine, Virginia Commonwealth University Veterans Affairs McGuire Medical Center, Richmond, VA 23249, USA
Metabolism 61:836-45. 2012..These findings support the hypothesis that 25HC3S is an important endogenous regulator of lipid biosynthesis... - alpha(1)-fetoprotein transcription factor (FTF)/liver receptor homolog-1 (LRH-1) is an essential lipogenic regulatorZhumei Xu
Department of Biochemistry and Molecular Biology, Medical College of Virginia at Virginia Commonwealth University, Richmond, Virginia 23298 0614, USA
Biochim Biophys Acta 1801:473-9. 2010..Taken together, our results identify the nuclear receptor FTF as a central regulator of lipid metabolism... - Identification of a novel sulfonated oxysterol, 5-cholesten-3beta,25-diol 3-sulfonate, in hepatocyte nuclei and mitochondriaShunlin Ren
Department of Medicine, Medical College of Virginia at Virginia Commonwealth University, Richmond, 23298, USA
J Lipid Res 47:1081-90. 2006..Further experiments detected this cholesterol metabolite in the nuclei of normal human liver tissues. Based upon these observations, we hypothesized a new pathway by which cholesterol is metabolized in the mitochondrion... - 25-Hydroxycholesterol-3-sulfate regulates macrophage lipid metabolism via the LXR/SREBP-1 signaling pathwayYongjie Ma
Veterans Affairs McGuire Medical Center Virginia Commonwealth University, Richmond, VA 23249, USA
Am J Physiol Endocrinol Metab 295:E1369-79. 2008..We conclude that 25HC3S acts in macrophages as a cholesterol satiety signal, downregulating cholesterol and fatty acid synthetic pathways via inhibition of LXR/SREBP signaling. A possible role of oxysterol sulfation is proposed... - Regulation of oxysterol 7alpha-hydroxylase (CYP7B1) in the ratShunlin Ren
Department of Medicine, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA 23249, USA
Metabolism 52:636-42. 2003..We conclude that, in the rat, like CYP7A1, CYP7B1 demonstrates diurnal rhythm and is regulated by bile acids and cholesterol... - 25-Hydroxycholesterol-3-sulfate attenuates inflammatory response via PPARγ signaling in human THP-1 macrophagesLeyuan Xu
Department of Medicine, Virginia Commonwealth University, Richmond, VA 23249, USA
Am J Physiol Endocrinol Metab 302:E788-99. 2012..We conclude that 25HC3S acts in macrophages as a PPARγ ligand and suppresses inflammatory responses via the PPARγ/IκB/NF-κB signaling pathway... - Sulfated oxysterol, 25HC3S, is a potent regulator of lipid metabolism in human hepatocytesShunlin Ren
Department of Medicine, Veterans Affairs McGuire Medical Center Virginia Commonwealth University, Richmond, VA 23249, USA
Biochem Biophys Res Commun 360:802-8. 2007..In contrast, 25-hydroxycholesterol increased SREBP1 and FAS mRNA levels in primary human hepatocytes. The results imply that 25HC3S is a potent regulator of SREBP mediated lipid metabolism... 
HIV protease inhibitors induce endoplasmic reticulum stress and disrupt barrier integrity in intestinal epithelial cellsXudong Wu
Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, Virginia 23298 0678, USA
Gastroenterology 138:197-209. 2010..However, the exact mechanisms underlying gastrointestinal adverse effects of HIV PIs remain unknown. This study investigated whether HIV PIs disrupt intestinal epithelial barrier integrity by activating endoplasmic reticulum (ER) stress...- Intracellular cholesterol transporter StarD4 binds free cholesterol and increases cholesteryl ester formationDaniel Rodriguez-Agudo
Department of Medicine, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA, USA
J Lipid Res 49:1409-19. 2008.... - HIV protease inhibitor lopinavir-induced TNF-alpha and IL-6 expression is coupled to the unfolded protein response and ERK signaling pathways in macrophagesLi Chen
Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23298, USA
Biochem Pharmacol 78:70-7. 2009.... - Regulation of hepatocyte lipid metabolism and inflammatory response by 25-hydroxycholesterol and 25-hydroxycholesterol-3-sulfateLeyuan Xu
Department of Medicine, McGuire Veterans Affairs Medical Center, Virginia Commonwealth University, Research 151, 1201 Broad Rock Blvd, Richmond, VA 23249, USA
Lipids 45:821-32. 2010..The current findings suggest that 25HC and 25HC3S serve as potent regulators in cross-talk of lipid metabolism and inflammatory response in the hepatocytes... - Human StarD5, a cytosolic StAR-related lipid binding proteinDaniel Rodriguez-Agudo
Department of Medicine, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA, USA
J Lipid Res 46:1615-23. 2005.... - Biosynthesis of the regulatory oxysterol, 5-cholesten-3beta,25-diol 3-sulfate, in hepatocytesXiaobo Li
Department of Medicine Veterans Affairs McGuire Medical Center, Virginia Commonwealth University, Richmond, VA 23249, USA
J Lipid Res 48:2587-96. 2007..Specific small interfering RNA decreased SULT2B1b mRNA, protein, and activity levels. These findings demonstrate that mitochondria synthesize 25HC, which is subsequently 3beta-sulfated to form 25HC3S... - Transport of cholesterol into mitochondria is rate-limiting for bile acid synthesis via the alternative pathway in primary rat hepatocytesWilliam M Pandak
Department of Medicine, Veterans Affairs Medical Center, and Virginia Commonwealth University, Richmond 23298 0711, USA
J Biol Chem 277:48158-64. 2002..These findings suggest that cholesterol delivery to the inner mitochondrial membrane is the predominant rate-determining step for bile acid synthesis via the alternative pathway... - 5-cholesten-3β,25-diol 3-sulfate decreases lipid accumulation in diet-induced nonalcoholic fatty liver disease mouse modelLeyuan Xu
McGuire Veterans Affairs Medical Center Virginia Commonwealth University, Research 151, Richmond, VA 23249, USA
Mol Pharmacol 83:648-58. 2013..The present results indicate that 25HC3S as a potent endogenous regulator decreases lipogenesis, and oxysterol sulfation can be a key protective regulatory pathway against lipid accumulation and lipid-induced inflammation in vivo... - Bile acids as regulatory moleculesPhillip B Hylemon
Department of Microbiology and Immunology, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, VA 23298 0678, USA
J Lipid Res 50:1509-20. 2009..In this review, we will summarize the current knowledge of how bile acids regulate hepatic lipid and glucose metabolism through the activation of specific nuclear receptors and cell signaling pathways... - Activation of bile acid biosynthesis by the p38 mitogen-activated protein kinase (MAPK): hepatocyte nuclear factor-4alpha phosphorylation by the p38 MAPK is required for cholesterol 7alpha-hydroxylase expressionZhumei Xu
Department of Biochemistry and Molecular Biology, Microbiology, Medical College of Virginia at Virginia Commonwealth University, Richmond, Virginia 23298 0614, USA
J Biol Chem 282:24607-14. 2007..These studies show a functional link between the p38 signaling pathway, HNF-4alpha, and bile acid synthesis... - HIV protease inhibitors activate the unfolded protein response and disrupt lipid metabolism in primary hepatocytesHuiping Zhou
Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, Virginia, USA
Am J Physiol Gastrointest Liver Physiol 291:G1071-80. 2006..The current results may help provide a better understanding of the cellular mechanisms of HIV PI-induced dyslipidemia and also provide useful information to help predict clinical adverse effects in the development of new HIV PIs... - Cytosolic sulfotransferase 2B1b promotes hepatocyte proliferation gene expression in vivo and in vitroXin Zhang
Department of Pathology, Fudan University Shanghai Medical College, Shanghai, China
Am J Physiol Gastrointest Liver Physiol 303:G344-55. 2012..LXR activation by T0901317 effectively suppressed SULT2B1b-induced gene expression in vivo and in vitro. SULT2B1b may promote hepatocyte proliferation by inactivating oxysterol/LXR signaling... - Conjugated bile acids activate the sphingosine-1-phosphate receptor 2 in primary rodent hepatocytesElaine Studer
Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23298 0678, USA
Hepatology 55:267-76. 2012..In summary, all these data support the hypothesis that conjugated bile acids activate the ERK1/2 and AKT signaling pathways primarily through S1P(2) in primary rodent hepatocytes... - Enzyme activity assay for cholesterol 27-hydroxylase in mitochondriaXiaobo Li
Department of Medicine, Division of Gastroenterology, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, USA
J Lipid Res 47:1507-12. 2006..Using this new assay, we found that during rat hepatocyte preparation and cell culture, mitochondria gradually lose CYP27A1 activity compared with mitochondria freshly isolated from rat liver tissue... - The role of alpha1-fetoprotein transcription factor/LRH-1 in bile acid biosynthesis: a known nuclear receptor activator that can act as a suppressor of bile acid biosynthesisAntonio Del Castillo-Olivares
Department of Biochemistry, Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia 23298 0614, USA
J Biol Chem 279:16813-21. 2004.... - Short course of omeprazole: a better first diagnostic approach to noncardiac chest pain than endoscopy, manometry, or 24-hour esophageal pH monitoringWilliam M Pandak
Division of Gastroenterology and Department of Biostatistics, Virginia Commonweath University, Richmond, Virginia 23249, USA
J Clin Gastroenterol 35:307-14. 2002..Noncardiac chest pain (NCCP) presents as a frequent diagnostic challenge, with patients tending to use a disproportionate level of health care resources. Gastroesophageal reflux disease (GERD) is the most frequent cause of NCCP... - HIV protease inhibitors activate the unfolded protein response in macrophages: implication for atherosclerosis and cardiovascular diseaseHuiping Zhou
Department of Microbiology and Immunology, Virginia Commonwealth University, P.O. Box 980678, Richmond, VA 23298-0678, USA
Mol Pharmacol 68:690-700. 2005..These findings provide possible cellular mechanisms by which HIV protease inhibitors promote atherosclerosis and cardiovascular disease in HIV-1 infected patients treated with HIV protease inhibitors... - LXR alpha is the dominant regulator of CYP7A1 transcriptionSeema Gupta
Department of Microbiology and Immunology, Virginia Commonwealth University, MCV Campus, Richmond, VA 23298, USA
Biochem Biophys Res Commun 293:338-43. 2002..These results suggest that under conditions of both SHP and LXR alpha activation, stimulatory effect of LXR alpha overrides the inhibitory effect of FXR and results in an induction of rat CYP7A1 mRNA levels... - Conjugated bile acids regulate hepatocyte glycogen synthase activity in vitro and in vivo via Galphai signalingYouwen Fang
Department of Biochemistry, Box 980035, Virginia Commonwealth University, Richmond VA 23298 0035, USA
Mol Pharmacol 71:1122-8. 2007.... - Prevention of free fatty acid-induced hepatic lipotoxicity by 18beta-glycyrrhetinic acid through lysosomal and mitochondrial pathwaysXudong Wu
Jiangsu Center for Drug Screening, Jiangsu Center for Pharmacodynamic Research and Evaluation, China Pharmaceutical University, Nanjing, Jiangsu, People s Republic of China
Hepatology 47:1905-15. 2008..CONCLUSION: GA significantly reduced FFA/HFD-induced hepatic lipotoxicity by stabilizing the integrity of lysosomes and mitochondria and inhibiting cathepsin B expression and enzyme activity...