William M Pandak

Summary

Country: USA

Publications

  1. ncbi request reprint Regulation of oxysterol 7alpha-hydroxylase (CYP7B1) in primary cultures of rat hepatocytes
    William M Pandak
    Department of Medicine, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA 23249, USA
    Hepatology 35:1400-8. 2002
  2. ncbi request reprint Localization of StarD5 cholesterol binding protein
    Daniel Rodriguez-Agudo
    Department of Medicine, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA, USA
    J Lipid Res 47:1168-75. 2006
  3. ncbi request reprint Overexpression of cholesterol transporter StAR increases in vivo rates of bile acid synthesis in the rat and mouse
    Shunlin Ren
    Department of Medicine, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA, USA
    Hepatology 40:910-7. 2004
  4. pmc 5-cholesten-3β,25-diol 3-sulfate decreases lipid accumulation in diet-induced nonalcoholic fatty liver disease mouse model
    Leyuan Xu
    McGuire Veterans Affairs Medical Center Virginia Commonwealth University, Research 151, Richmond, VA 23249, USA
    Mol Pharmacol 83:648-58. 2013
  5. pmc Subcellular localization and regulation of StarD4 protein in macrophages and fibroblasts
    Daniel Rodriguez-Agudo
    Department of Medicine, Virginia Commonwealth University, Richmond, VA, USA
    Biochim Biophys Acta 1811:597-606. 2011
  6. pmc Cholesterol metabolite, 5-cholesten-3β-25-diol-3-sulfate, promotes hepatic proliferation in mice
    Xin Zhang
    Department of Medicine, Virginia Commonwealth University Veterans Affairs McGuire Medical Center, 1201 Broad Rock Boulevard, Richmond, VA 23249, United States
    J Steroid Biochem Mol Biol 132:262-70. 2012
  7. ncbi request reprint Effect of increasing the expression of cholesterol transporters (StAR, MLN64, and SCP-2) on bile acid synthesis
    Shunlin Ren
    Department of Medicine, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA, USA
    J Lipid Res 45:2123-31. 2004
  8. pmc Sulfation of 25-hydroxycholesterol by SULT2B1b decreases cellular lipids via the LXR/SREBP-1c signaling pathway in human aortic endothelial cells
    Qianming Bai
    Department of Medicine, Virginia Commonwealth University Veterans Affairs McGuire Medical Center, Richmond, VA 23249, United States
    Atherosclerosis 214:350-6. 2011
  9. pmc Bile acids regulate hepatic gluconeogenic genes and farnesoid X receptor via G(alpha)i-protein-coupled receptors and the AKT pathway
    Risheng Cao
    Departments of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23298, USA
    J Lipid Res 51:2234-44. 2010
  10. pmc Intracellular cholesterol transporter StarD4 binds free cholesterol and increases cholesteryl ester formation
    Daniel Rodriguez-Agudo
    Department of Medicine, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA, USA
    J Lipid Res 49:1409-19. 2008

Collaborators

Detail Information

Publications37

  1. ncbi request reprint Regulation of oxysterol 7alpha-hydroxylase (CYP7B1) in primary cultures of rat hepatocytes
    William M Pandak
    Department of Medicine, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA 23249, USA
    Hepatology 35:1400-8. 2002
    ..In conclusion, in the rat, CYP7B1 specific activity is highly regulated but does not seem to be rate limiting for bile acid synthesis...
  2. ncbi request reprint Localization of StarD5 cholesterol binding protein
    Daniel Rodriguez-Agudo
    Department of Medicine, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA, USA
    J Lipid Res 47:1168-75. 2006
    ..The presence of this cholesterol/25-hydroxycholesterol-binding protein in cells related to inflammatory processes provides new clues to the role of this protein in free sterol transport in the cells and in lipid-mediated atherogenesis...
  3. ncbi request reprint Overexpression of cholesterol transporter StAR increases in vivo rates of bile acid synthesis in the rat and mouse
    Shunlin Ren
    Department of Medicine, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA, USA
    Hepatology 40:910-7. 2004
    ..01). In conclusion, in both rats and mice in vivo, overexpression of StAR led to a marked increase in the rates of BAS initiated by delivery of cholesterol to mitochondria containing CYP27A1...
  4. pmc 5-cholesten-3β,25-diol 3-sulfate decreases lipid accumulation in diet-induced nonalcoholic fatty liver disease mouse model
    Leyuan Xu
    McGuire Veterans Affairs Medical Center Virginia Commonwealth University, Research 151, Richmond, VA 23249, USA
    Mol Pharmacol 83:648-58. 2013
    ..The present results indicate that 25HC3S as a potent endogenous regulator decreases lipogenesis, and oxysterol sulfation can be a key protective regulatory pathway against lipid accumulation and lipid-induced inflammation in vivo...
  5. pmc Subcellular localization and regulation of StarD4 protein in macrophages and fibroblasts
    Daniel Rodriguez-Agudo
    Department of Medicine, Virginia Commonwealth University, Richmond, VA, USA
    Biochim Biophys Acta 1811:597-606. 2011
    ....
  6. pmc Cholesterol metabolite, 5-cholesten-3β-25-diol-3-sulfate, promotes hepatic proliferation in mice
    Xin Zhang
    Department of Medicine, Virginia Commonwealth University Veterans Affairs McGuire Medical Center, 1201 Broad Rock Boulevard, Richmond, VA 23249, United States
    J Steroid Biochem Mol Biol 132:262-70. 2012
    ..Conclusions: 25HC3S may be a potent regulator of hepatocyte proliferation and oxysterol sulfation may represent a novel regulatory pathway in liver proliferation via inactivating LXR signaling...
  7. ncbi request reprint Effect of increasing the expression of cholesterol transporters (StAR, MLN64, and SCP-2) on bile acid synthesis
    Shunlin Ren
    Department of Medicine, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA, USA
    J Lipid Res 45:2123-31. 2004
    ..These results suggest that MLN64, in its full-length form, is not responsible for the transport of cholesterol to the mitochondria or the endoplasmic reticulum, where CYP27A1 or CYP7A1 is located, respectively...
  8. pmc Sulfation of 25-hydroxycholesterol by SULT2B1b decreases cellular lipids via the LXR/SREBP-1c signaling pathway in human aortic endothelial cells
    Qianming Bai
    Department of Medicine, Virginia Commonwealth University Veterans Affairs McGuire Medical Center, Richmond, VA 23249, United States
    Atherosclerosis 214:350-6. 2011
    ..We evaluate the effect of 25HC sulfation on lipid metabolism by overexpressing the gene encoding SULT2B1b in human aortic endothelial cells (HAECs) in culture...
  9. pmc Bile acids regulate hepatic gluconeogenic genes and farnesoid X receptor via G(alpha)i-protein-coupled receptors and the AKT pathway
    Risheng Cao
    Departments of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23298, USA
    J Lipid Res 51:2234-44. 2010
    ..Activation of the insulin signaling pathway appears to be linked to the upregulation of farnesoid X receptor functional activity and SHP induction...
  10. pmc Intracellular cholesterol transporter StarD4 binds free cholesterol and increases cholesteryl ester formation
    Daniel Rodriguez-Agudo
    Department of Medicine, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA, USA
    J Lipid Res 49:1409-19. 2008
    ....
  11. pmc Oxysterol sulfation by cytosolic sulfotransferase suppresses liver X receptor/sterol regulatory element binding protein-1c signaling pathway and reduces serum and hepatic lipids in mouse models of nonalcoholic fatty liver disease
    Qianming Bai
    Department of Medicine, Virginia Commonwealth University Veterans Affairs McGuire Medical Center, Richmond, VA 23249, USA
    Metabolism 61:836-45. 2012
    ..These findings support the hypothesis that 25HC3S is an important endogenous regulator of lipid biosynthesis...
  12. pmc alpha(1)-fetoprotein transcription factor (FTF)/liver receptor homolog-1 (LRH-1) is an essential lipogenic regulator
    Zhumei Xu
    Department of Biochemistry and Molecular Biology, Medical College of Virginia at Virginia Commonwealth University, Richmond, Virginia 23298 0614, USA
    Biochim Biophys Acta 1801:473-9. 2010
    ..Taken together, our results identify the nuclear receptor FTF as a central regulator of lipid metabolism...
  13. pmc 25-Hydroxycholesterol-3-sulfate regulates macrophage lipid metabolism via the LXR/SREBP-1 signaling pathway
    Yongjie Ma
    Veterans Affairs McGuire Medical Center Virginia Commonwealth University, Richmond, VA 23249, USA
    Am J Physiol Endocrinol Metab 295:E1369-79. 2008
    ..We conclude that 25HC3S acts in macrophages as a cholesterol satiety signal, downregulating cholesterol and fatty acid synthetic pathways via inhibition of LXR/SREBP signaling. A possible role of oxysterol sulfation is proposed...
  14. ncbi request reprint Regulation of oxysterol 7alpha-hydroxylase (CYP7B1) in the rat
    Shunlin Ren
    Department of Medicine, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA 23249, USA
    Metabolism 52:636-42. 2003
    ..We conclude that, in the rat, like CYP7A1, CYP7B1 demonstrates diurnal rhythm and is regulated by bile acids and cholesterol...
  15. ncbi request reprint Identification of a novel sulfonated oxysterol, 5-cholesten-3beta,25-diol 3-sulfonate, in hepatocyte nuclei and mitochondria
    Shunlin Ren
    Department of Medicine, Medical College of Virginia at Virginia Commonwealth University, Richmond, 23298, USA
    J Lipid Res 47:1081-90. 2006
    ..Further experiments detected this cholesterol metabolite in the nuclei of normal human liver tissues. Based upon these observations, we hypothesized a new pathway by which cholesterol is metabolized in the mitochondrion...
  16. pmc 25-Hydroxycholesterol-3-sulfate attenuates inflammatory response via PPARγ signaling in human THP-1 macrophages
    Leyuan Xu
    Department of Medicine, Virginia Commonwealth University, Richmond, VA 23249, USA
    Am J Physiol Endocrinol Metab 302:E788-99. 2012
    ..We conclude that 25HC3S acts in macrophages as a PPARγ ligand and suppresses inflammatory responses via the PPARγ/IκB/NF-κB signaling pathway...
  17. pmc Sulfated oxysterol, 25HC3S, is a potent regulator of lipid metabolism in human hepatocytes
    Shunlin Ren
    Department of Medicine, Veterans Affairs McGuire Medical Center Virginia Commonwealth University, Richmond, VA 23249, USA
    Biochem Biophys Res Commun 360:802-8. 2007
    ..In contrast, 25-hydroxycholesterol increased SREBP1 and FAS mRNA levels in primary human hepatocytes. The results imply that 25HC3S is a potent regulator of SREBP mediated lipid metabolism...
  18. doi request reprint HIV protease inhibitors induce endoplasmic reticulum stress and disrupt barrier integrity in intestinal epithelial cells
    Xudong Wu
    Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, Virginia 23298 0678, USA
    Gastroenterology 138:197-209. 2010
    ..However, the exact mechanisms underlying gastrointestinal adverse effects of HIV PIs remain unknown. This study investigated whether HIV PIs disrupt intestinal epithelial barrier integrity by activating endoplasmic reticulum (ER) stress...
  19. pmc The role of CCAAT enhancer-binding protein homologous protein in human immunodeficiency virus protease-inhibitor-induced hepatic lipotoxicity in mice
    Yun Wang
    Department of Microbiology and Immunology, School of Medicine, Virginia Commonwealth University, Richmond, VA 23298 0678, USA
    Hepatology 57:1005-16. 2013
    ..hepatology 2013)...
  20. pmc HIV protease inhibitor lopinavir-induced TNF-alpha and IL-6 expression is coupled to the unfolded protein response and ERK signaling pathways in macrophages
    Li Chen
    Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23298, USA
    Biochem Pharmacol 78:70-7. 2009
    ....
  21. doi request reprint Regulation of hepatocyte lipid metabolism and inflammatory response by 25-hydroxycholesterol and 25-hydroxycholesterol-3-sulfate
    Leyuan Xu
    Department of Medicine, McGuire Veterans Affairs Medical Center, Virginia Commonwealth University, Research 151, 1201 Broad Rock Blvd, Richmond, VA 23249, USA
    Lipids 45:821-32. 2010
    ..The current findings suggest that 25HC and 25HC3S serve as potent regulators in cross-talk of lipid metabolism and inflammatory response in the hepatocytes...
  22. pmc ER stress increases StarD5 expression by stabilizing its mRNA and leads to relocalization of its protein from the nucleus to the membranes
    Daniel Rodriguez-Agudo
    Department of Medicine, Virginia Commonwealth University, Richmond, VA, USA
    J Lipid Res 53:2708-15. 2012
    ..These results reveal the increase in StarD5 expression and protein redistribution during the cell protective phase of the ER stress, suggesting a role for StarD5 in cholesterol metabolism during the ER stress response...
  23. ncbi request reprint Transport of cholesterol into mitochondria is rate-limiting for bile acid synthesis via the alternative pathway in primary rat hepatocytes
    William M Pandak
    Department of Medicine, Veterans Affairs Medical Center, and Virginia Commonwealth University, Richmond 23298 0711, USA
    J Biol Chem 277:48158-64. 2002
    ..These findings suggest that cholesterol delivery to the inner mitochondrial membrane is the predominant rate-determining step for bile acid synthesis via the alternative pathway...
  24. ncbi request reprint Biosynthesis of the regulatory oxysterol, 5-cholesten-3beta,25-diol 3-sulfate, in hepatocytes
    Xiaobo Li
    Department of Medicine Veterans Affairs McGuire Medical Center, Virginia Commonwealth University, Richmond, VA 23249, USA
    J Lipid Res 48:2587-96. 2007
    ..Specific small interfering RNA decreased SULT2B1b mRNA, protein, and activity levels. These findings demonstrate that mitochondria synthesize 25HC, which is subsequently 3beta-sulfated to form 25HC3S...
  25. ncbi request reprint Human StarD5, a cytosolic StAR-related lipid binding protein
    Daniel Rodriguez-Agudo
    Department of Medicine, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA, USA
    J Lipid Res 46:1615-23. 2005
    ....
  26. pmc Activation of bile acid biosynthesis by the p38 mitogen-activated protein kinase (MAPK): hepatocyte nuclear factor-4alpha phosphorylation by the p38 MAPK is required for cholesterol 7alpha-hydroxylase expression
    Zhumei Xu
    Department of Biochemistry and Molecular Biology, Microbiology, Medical College of Virginia at Virginia Commonwealth University, Richmond, Virginia 23298 0614, USA
    J Biol Chem 282:24607-14. 2007
    ..These studies show a functional link between the p38 signaling pathway, HNF-4alpha, and bile acid synthesis...
  27. pmc Bile acids as regulatory molecules
    Phillip B Hylemon
    Department of Microbiology and Immunology, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, VA 23298 0678, USA
    J Lipid Res 50:1509-20. 2009
    ..In this review, we will summarize the current knowledge of how bile acids regulate hepatic lipid and glucose metabolism through the activation of specific nuclear receptors and cell signaling pathways...
  28. ncbi request reprint HIV protease inhibitors activate the unfolded protein response and disrupt lipid metabolism in primary hepatocytes
    Huiping Zhou
    Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, Virginia, USA
    Am J Physiol Gastrointest Liver Physiol 291:G1071-80. 2006
    ..The current results may help provide a better understanding of the cellular mechanisms of HIV PI-induced dyslipidemia and also provide useful information to help predict clinical adverse effects in the development of new HIV PIs...
  29. pmc Cytosolic sulfotransferase 2B1b promotes hepatocyte proliferation gene expression in vivo and in vitro
    Xin Zhang
    Department of Pathology, Fudan University Shanghai Medical College, Shanghai, China
    Am J Physiol Gastrointest Liver Physiol 303:G344-55. 2012
    ..LXR activation by T0901317 effectively suppressed SULT2B1b-induced gene expression in vivo and in vitro. SULT2B1b may promote hepatocyte proliferation by inactivating oxysterol/LXR signaling...
  30. pmc Conjugated bile acids activate the sphingosine-1-phosphate receptor 2 in primary rodent hepatocytes
    Elaine Studer
    Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23298 0678, USA
    Hepatology 55:267-76. 2012
    ..In summary, all these data support the hypothesis that conjugated bile acids activate the ERK1/2 and AKT signaling pathways primarily through S1P(2) in primary rodent hepatocytes...
  31. ncbi request reprint The role of alpha1-fetoprotein transcription factor/LRH-1 in bile acid biosynthesis: a known nuclear receptor activator that can act as a suppressor of bile acid biosynthesis
    Antonio Del Castillo-Olivares
    Department of Biochemistry, Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia 23298 0614, USA
    J Biol Chem 279:16813-21. 2004
    ....
  32. ncbi request reprint Enzyme activity assay for cholesterol 27-hydroxylase in mitochondria
    Xiaobo Li
    Department of Medicine, Division of Gastroenterology, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, USA
    J Lipid Res 47:1507-12. 2006
    ..Using this new assay, we found that during rat hepatocyte preparation and cell culture, mitochondria gradually lose CYP27A1 activity compared with mitochondria freshly isolated from rat liver tissue...
  33. ncbi request reprint Short course of omeprazole: a better first diagnostic approach to noncardiac chest pain than endoscopy, manometry, or 24-hour esophageal pH monitoring
    William M Pandak
    Division of Gastroenterology and Department of Biostatistics, Virginia Commonweath University, Richmond, Virginia 23249, USA
    J Clin Gastroenterol 35:307-14. 2002
    ..Noncardiac chest pain (NCCP) presents as a frequent diagnostic challenge, with patients tending to use a disproportionate level of health care resources. Gastroesophageal reflux disease (GERD) is the most frequent cause of NCCP...
  34. ncbi request reprint HIV protease inhibitors activate the unfolded protein response in macrophages: implication for atherosclerosis and cardiovascular disease
    Huiping Zhou
    Department of Microbiology and Immunology, Virginia Commonwealth University, P O Box 980678, Richmond, VA 23298 0678, USA
    Mol Pharmacol 68:690-700. 2005
    ..These findings provide possible cellular mechanisms by which HIV protease inhibitors promote atherosclerosis and cardiovascular disease in HIV-1 infected patients treated with HIV protease inhibitors...
  35. ncbi request reprint LXR alpha is the dominant regulator of CYP7A1 transcription
    Seema Gupta
    Department of Microbiology and Immunology, Virginia Commonwealth University, MCV Campus, Richmond, VA 23298, USA
    Biochem Biophys Res Commun 293:338-43. 2002
    ..These results suggest that under conditions of both SHP and LXR alpha activation, stimulatory effect of LXR alpha overrides the inhibitory effect of FXR and results in an induction of rat CYP7A1 mRNA levels...
  36. doi request reprint Prevention of free fatty acid-induced hepatic lipotoxicity by 18beta-glycyrrhetinic acid through lysosomal and mitochondrial pathways
    Xudong Wu
    Jiangsu Center for Drug Screening, Jiangsu Center for Pharmacodynamic Research and Evaluation, China Pharmaceutical University, Nanjing, Jiangsu, People s Republic of China
    Hepatology 47:1905-15. 2008
    ..These characteristics may represent major cellular mechanisms, which account for its protective effects on FFA/HFD-induced hepatic lipotoxicity...
  37. pmc Conjugated bile acids regulate hepatocyte glycogen synthase activity in vitro and in vivo via Galphai signaling
    Youwen Fang
    Department of Biochemistry, Box 980035, Virginia Commonwealth University, Richmond VA 23298 0035, USA
    Mol Pharmacol 71:1122-8. 2007
    ....