Research Topics
| V PoitoutSummaryAffiliation: Pacific Northwest Research Institute Country: USA Publications
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Detail Information
Publications
The ins and outs of fatty acids on the pancreatic beta cellVincent Poitout
Pacific Northwest Research Institute and Department of Medicine, University of Washington, Seattle, WA, USA
Trends Endocrinol Metab 14:201-3. 2003- Gluco-lipotoxicity of the pancreatic beta cellV Poitout
Pacific Northwest Research Institute, 720 Broadway, Seattle, WA 98122, USA
Ann Endocrinol (Paris) 65:37-41. 2004 - Minireview: Secondary beta-cell failure in type 2 diabetes--a convergence of glucotoxicity and lipotoxicityVincent Poitout
Pacific Northwest Research Institute, Seattle, Washington 98122, USA
Endocrinology 143:339-42. 2002..We propose that chronic hyperglycemia, independent of hyperlipidemia, is toxic for beta-cell function, whereas chronic hyperlipidemia is deleterious only in the context of concomitant hyperglycemia... - Glucose-induced insulin mRNA accumulation is impaired in islets from neonatal streptozotocin-treated ratsI Briaud
Pacific Northwest Research Institute, Seattle, WA 98122, USA
Horm Metab Res 32:103-6. 2000..In contrast, no significant effect of glucose was found on insulin mRNA levels in n0-STZ islets. We conclude that insulin gene regulation by glucose is impaired in n0-STZ rat islets... - Glucose-induced insulin mRNA accumulation is impaired in islets from neonatal streptozotocin-treated ratsI Briaud
Pacific Northwest Research Institute, Seattle WA, USA
Horm Metab Res 32:53-6. 2000..In contrast, no significant effect of glucose was found on insulin mRNA levels in n0-STZ islets. We conclude that insulin gene regulation by glucose is impaired in n0-STZ rat islets... - Does leptin regulate insulin secretion?V Poitout
Pacific Northwest Research Institute, Seattle, WA 98122, USA
Diabetes Metab 24:321-6. 1998.... - Antecedent hyperglycemia, not hyperlipidemia, is associated with increased islet triacylglycerol content and decreased insulin gene mRNA level in Zucker diabetic fatty ratsJ S Harmon
Pacific Northwest Research Institute and the Departments of Medicine and Pharmacology, University of Washington, Seattle, Washington 98122, USA
Diabetes 50:2481-6. 2001..We conclude that antecedent elevated plasma glucose levels, not plasma lipid levels, are associated with elevated islet TAG content and decreased insulin mRNA levels in ZDF animals... - Palmitate potentiation of glucose-induced insulin release: a study using 2-bromopalmitateS M Parker
Pacific Nortwest Research Institute, Seattle, WA 98122, USA
Metabolism 52:1367-71. 2003..Further, 2-bromopalmitate partly inhibited the potentiating effect of palmitate. These results support the concept that FA potentiation of insulin release is mediated by FA-derived signals generated in the esterification pathway... - Pancreatic islet response to hyperglycemia is dependent on peroxisome proliferator-activated receptor alpha (PPARalpha)Hélène Bihan
INSERM U 341, Hotel Dieu, Paris, France
FEBS Lett 579:2284-8. 2005..As such, islet PPARalpha could contribute to delay the progression of type 2 diabetes... 
Palmitate inhibits insulin gene expression by altering PDX-1 nuclear localization and reducing MafA expression in isolated rat islets of LangerhansDerek K Hagman
Pacific Northwest Research Institute, Seattle, Washington 98122, USA
J Biol Chem 280:32413-8. 2005..These results demonstrate that prolonged exposure of islets to palmitate inhibits insulin gene transcription by impairing nuclear localization of PDX-1 and cellular expression of MafA...- Regulation of the insulin gene by glucose and fatty acidsVincent Poitout
Department of Medicine, University of Montreal, Montreal, QC, Canada
J Nutr 136:873-6. 2006..Glucotoxicity and lipotoxicity have common targets, which makes their combination particularly harmful to insulin gene expression and beta-cell function in type 2 diabetes... - GPR40 is necessary but not sufficient for fatty acid stimulation of insulin secretion in vivoMartin G Latour
Centre de recherche du Centre Hospitalier de l Universite de Montreal, Technopole Angus, 2901 Rachel Est, Montreal, QC, H1W 4A4 Canada
Diabetes 56:1087-94. 2007..We conclude that GPR40 contributes approximately half of the full acute insulin secretory response to fatty acids in mice but does not play a role in the mechanisms by which fatty acids chronically impair insulin secretion... - G protein-coupled receptors and insulin secretion: 119 and countingS R Murthy Madiraju
Montreal Diabetes Research Centre, University of Montreal, 2901 Rachel East, Montreal, Quebec, Canada
Endocrinology 148:2598-600. 2007 - Cyclical and alternating infusions of glucose and intralipid in rats inhibit insulin gene expression and Pdx-1 binding in isletsDerek K Hagman
DVM, Montreal Diabetes Research Center, Centre de recherche du Centre Hospitalier de l Universite de Montreal, Technopole Angus, 2901 Rachel Est, Montreal, QC, H1W 4A4, Canada
Diabetes 57:424-31. 2008..Whether such a phenomenon also occurs in vivo is unknown. Our objective was therefore to ascertain whether chronic nutrient oversupply inhibits insulin gene expression in vivo... - The fatty acid receptor GPR40 plays a role in insulin secretion in vivo after high-fat feedingMelkam Kebede
Montreal Diabetes Research Center, Centre de recherche du Centre Hospitalier de l Universite de Montreal, Montreal, Canada
Diabetes 57:2432-7. 2008..Glucose tolerance, insulin tolerance, and insulin secretion in response to glucose and Intralipid were assessed during the course of the diet period... - Elevated glucose attenuates human insulin gene promoter activity in INS-1 pancreatic beta-cells via reduced nuclear factor binding to the A5/core and Z elementMaria F Pino
Department of Physiology, Michigan State University, 3176 Biomedical and Physical Sciences Building, East Lansing, MI 48824, USA
Mol Endocrinol 19:1343-60. 2005.... - Glucolipotoxicity of the pancreatic beta-cell: myth or reality?Vincent Poitout
Montreal Diabetes Research Center, Centre de recherche du Centre Hospitalier de l Universite de Montreal, Technopole Angus, 2901 Rachel Est, Montreal, QC, Canada, H1W 4A4
Biochem Soc Trans 36:901-4. 2008.... - Differential effects of hyperlipidemia on insulin secretion in islets of langerhans from hyperglycemic versus normoglycemic ratsIsabelle Briaud
Pacific Northwest Research Institute, Seattle, Washington 98122, USA
Diabetes 51:662-8. 2002..We conclude that hyperlipidemia induced by high-fat feeding affects insulin secretion in islets from hyperglycemic GK rats only, by a mechanism which may involve, at least in part, modulation of UCP-2 expression... - Increasing triglyceride synthesis inhibits glucose-induced insulin secretion in isolated rat islets of langerhans: a study using adenoviral expression of diacylglycerol acyltransferaseCynthia L Kelpe
Pacific Northwest Research Institute, Seattle, Washington 98122, USA
Endocrinology 143:3326-32. 2002..We conclude that a selective increase in TG content impairs glucose-induced insulin secretion, a mechanism likely to mediate, at least in part, the deleterious effects of FA on pancreatic beta-cell function... - Insulin secretory deficiency and glucose intolerance in Rab3A null miceKazuro Yaekura
Pacific Northwest Research Institute and Departments of Pharmacology and Medicine, University of Washington, Seattle, Washington 98122, USA
J Biol Chem 278:9715-21. 2003..Also, this study indicates, for the first time, that the in vivo insulin secretory dysfunction found in type-2 diabetes can lie solely at the level of defective insulin exocytosis... - Palmitate inhibition of insulin gene expression is mediated at the transcriptional level via ceramide synthesisCynthia L Kelpe
Pacific Northwest Research Institute, Seattle, Washington 98122, USA
J Biol Chem 278:30015-21. 2003..We conclude that prolonged exposure to elevated levels of palmitate affects glucose-stimulated insulin gene expression via transcriptional mechanisms and ceramide synthesis... - Beta-cell glucose toxicity, lipotoxicity, and chronic oxidative stress in type 2 diabetesR Paul Robertson
Pacific Northwest Research Institute and the Departments of Medicine and Pharmacology, University of Washington, Seattle, Washington 98122, USA
Diabetes 53:S119-24. 2004.... - A role for the malonyl-CoA/long-chain acyl-CoA pathway of lipid signaling in the regulation of insulin secretion in response to both fuel and nonfuel stimuliRaphael Roduit
Molecular Nutrition Unit, Department of Nutrition, University of Montréal and the Centre Hospitalier de l Université de Montréal, Montreal, Quebec, Canada
Diabetes 53:1007-19. 2004..The data show the existence of important links between the metabolic coupling factor malonyl-CoA, the partitioning of fatty acids, and the stimulation of insulin secretion to both fuel and nonfuel stimuli... - Beta-cell lipotoxicity: burning fat into heat?Vincent Poitout
Endocrinology 145:3563-5. 2004 - Characterization of the human pancreatic islet proteome by two-dimensional LC/MS/MSThomas O Metz
Biological Science Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, USA
J Proteome Res 5:3345-54. 2006..The resulting peptide reference library provides a resource for future higher throughput and quantitative studies of islet biology... - Effect of the two-layer (University of Wisconsin solution-perfluorochemical plus O2) method of pancreas preservation on human islet isolation, as assessed by the Edmonton Isolation ProtocolShinichi Matsumoto
Islet and Cell Processing Laboratory ICPL at Puget Sound Blood Center and Northwest Tissue Center, Seattle, WA, USA
Transplantation 74:1414-9. 2002..In this study, we hypothesized that the two-layer method (TLM) could extend the acceptable preservation period of pancreata before islet isolation and increase islet yields... - Phospholipid hydrolysis and insulin secretion: a step toward solving the Rubik's cubeVincent Poitout
Montreal Diabetes Research Center, CR-CHUM, Technopole Angus, 2901 Rachel Est, Montreal, Quebec, Canada
Am J Physiol Endocrinol Metab 294:E214-6. 2008 - The islet beta cell-enriched MafA activator is a key regulator of insulin gene transcriptionLi Zhao
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
J Biol Chem 280:11887-94. 2005..Collectively, these results suggest that MafA plays a key role in coordinating and controlling the level of insulin gene expression in islet beta cells... - Glucolipotoxicity: fuel excess and beta-cell dysfunctionVincent Poitout
Montreal Diabetes Research Center, CR CHUM, Technopole Angus, 2901 Rachel Est, Montreal, Quebec, Canada H1W 4A4
Endocr Rev 29:351-66. 2008..Finally, we attempt to define the role of these phenomena in the natural history of beta-cell compensation, decompensation, and failure during the course of type 2 diabetes... - Evidence against the involvement of oxidative stress in fatty acid inhibition of insulin secretionPatrick C Moore
Pacific Northwest Research Institute, 720 Broadway, Seattle, WA 98122, USA
Diabetes 53:2610-6. 2004....