Chenghong Lei

Summary

Affiliation: Pacific Northwest National Laboratory
Country: USA

Publications

  1. pmc Local release of highly loaded antibodies from functionalized nanoporous support for cancer immunotherapy
    Chenghong Lei
    Pacific Northwest National Laboratory, Richland, Washington 99352, USA
    J Am Chem Soc 132:6906-7. 2010
  2. ncbi request reprint Synergetic effects of nanoporous support and urea on enzyme activity
    Chenghong Lei
    Pacific Northwest National Laboratory, Richland, Washington 99352, USA
    Nano Lett 7:1050-3. 2007
  3. pmc Probing mechanisms for enzymatic activity enhancement of organophosphorus hydrolase in functionalized mesoporous silica
    Baowei Chen
    Pacific Northwest National Laboratory, Richland, WA 99352, USA
    Biochem Biophys Res Commun 390:1177-81. 2009
  4. pmc In vitro release of organophosphorus acid anhydrolase from functionalized mesoporous silica against nerve agents
    Baowei Chen
    Pacific Northwest National Laboratory, Richland, WA 99352, USA
    Anal Biochem 421:477-81. 2012
  5. pmc The role of nonbonded interactions in the conformational dynamics of organophosphorous hydrolase adsorbed onto functionalized mesoporous silica surfaces
    Diego E B Gomes
    Pacific Northwest National Laboratory, P O Box 999, MSIN K7 90, Richland, Washington 99352, USA
    J Phys Chem B 114:531-40. 2010
  6. ncbi request reprint Biosensing paraoxon in simulated environmental samples by immobilized organophosphorus hydrolase in functionalized mesoporous silica
    Chenghong Lei
    Pacific Northwest National Laboratory, P O Box 999, Richland, WA 99352, USA
    J Environ Qual 36:233-8. 2007
  7. ncbi request reprint Enzyme specific activity in functionalized nanoporous supports
    Chenghong Lei
    Pacific Northwest National Laboratory, PO Box 999, Richland, WA 99352, USA
    Nanotechnology 19:125102. 2008
  8. pmc In situ regeneration of NADH via lipoamide dehydrogenase-catalyzed electron transfer reaction evidenced by spectroelectrochemistry
    Tsz Kin Tam
    Pacific Northwest National Laboratory, Richland, WA 99352, USA
    Bioelectrochemistry 86:92-6. 2012
  9. doi request reprint Heated proteins are still active in a functionalized nanoporous support
    Baowei Chen
    Pacific Northwest National Laboratory, PO Box 999, Richland, WA 99352, USA
    Small 9:2228-32. 2013
  10. ncbi request reprint Entrapping enzyme in a functionalized nanoporous support
    Chenghong Lei
    Pacific Northwest National Laboratory, Richland, Washington 99352, USA
    J Am Chem Soc 124:11242-3. 2002

Collaborators

  • Jun Liu
  • Baowei Chen
  • Tsz Kin Tam
  • Yongsoon Shin
  • Diego E B Gomes
  • Wen Qi
  • Xiaolin Li
  • Saumil S Shah
  • Roberto D Lins
  • Pedro G Pascutti
  • Thereza A Soares

Detail Information

Publications10

  1. pmc Local release of highly loaded antibodies from functionalized nanoporous support for cancer immunotherapy
    Chenghong Lei
    Pacific Northwest National Laboratory, Richland, Washington 99352, USA
    J Am Chem Soc 132:6906-7. 2010
    ..Our findings open up a novel approach for local delivery of therapeutically active proteins to tumors and, potentially, other diseases...
  2. ncbi request reprint Synergetic effects of nanoporous support and urea on enzyme activity
    Chenghong Lei
    Pacific Northwest National Laboratory, Richland, Washington 99352, USA
    Nano Lett 7:1050-3. 2007
    ..Rather than losing all activity in a denaturing solution of 8.0 M urea, the specific activity of GI entrapped in FMS remained higher than the highest specific activity of GI free in solution...
  3. pmc Probing mechanisms for enzymatic activity enhancement of organophosphorus hydrolase in functionalized mesoporous silica
    Baowei Chen
    Pacific Northwest National Laboratory, Richland, WA 99352, USA
    Biochem Biophys Res Commun 390:1177-81. 2009
    ..These results suggest the interactions of OPH with HOOC-FMS resulted in the protein immobilization and a favorable conformational change for OPH in the crowded confinement space and accordingly the enhanced activity...
  4. pmc In vitro release of organophosphorus acid anhydrolase from functionalized mesoporous silica against nerve agents
    Baowei Chen
    Pacific Northwest National Laboratory, Richland, WA 99352, USA
    Anal Biochem 421:477-81. 2012
    ..The in vitro results in the rabbit serum demonstrate that both OPAA-FMS and the released OPAA may be used as a medical countermeasure against the organophosphorus nerve agents...
  5. pmc The role of nonbonded interactions in the conformational dynamics of organophosphorous hydrolase adsorbed onto functionalized mesoporous silica surfaces
    Diego E B Gomes
    Pacific Northwest National Laboratory, P O Box 999, MSIN K7 90, Richland, Washington 99352, USA
    J Phys Chem B 114:531-40. 2010
    ..In addition, it is found that OPH adsorption onto the FMS models favors a protein conformation whose active site is fully accessible to the substrate, in contrast to the unconfined protein...
  6. ncbi request reprint Biosensing paraoxon in simulated environmental samples by immobilized organophosphorus hydrolase in functionalized mesoporous silica
    Chenghong Lei
    Pacific Northwest National Laboratory, P O Box 999, Richland, WA 99352, USA
    J Environ Qual 36:233-8. 2007
    ....
  7. ncbi request reprint Enzyme specific activity in functionalized nanoporous supports
    Chenghong Lei
    Pacific Northwest National Laboratory, PO Box 999, Richland, WA 99352, USA
    Nanotechnology 19:125102. 2008
    ..In contrast, substrate access to OPH molecules in FMS remains open at high P(LD) and may promote a more favorable confinement environment that enhances the OPH activity...
  8. pmc In situ regeneration of NADH via lipoamide dehydrogenase-catalyzed electron transfer reaction evidenced by spectroelectrochemistry
    Tsz Kin Tam
    Pacific Northwest National Laboratory, Richland, WA 99352, USA
    Bioelectrochemistry 86:92-6. 2012
    ....
  9. doi request reprint Heated proteins are still active in a functionalized nanoporous support
    Baowei Chen
    Pacific Northwest National Laboratory, PO Box 999, Richland, WA 99352, USA
    Small 9:2228-32. 2013
    ..Surprisingly, the protein released from the heated protein-nanoporous composite can maintain its nearly native conformation and activity, while free proteins are permanently denatured under the same treatment...
  10. ncbi request reprint Entrapping enzyme in a functionalized nanoporous support
    Chenghong Lei
    Pacific Northwest National Laboratory, Richland, Washington 99352, USA
    J Am Chem Soc 124:11242-3. 2002
    ..The combination of high protein loading, high immobilization efficiency and stability is attributed to the large and uniform pore structure, and to the optimum environment introduced by the functional groups...

Research Grants3