Research Topics
| PETER A ZUBERSummaryAffiliation: Oregon Health and Science University Country: USA Publications
Research Grants
| Collaborators
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Detail Information
Publications
Evidence that a single monomer of Spx can productively interact with RNA polymerase in Bacillus subtilisAnn A Lin
Division of Environmental and Biomolecular Systems, Institute of Environmental Health, Oregon Health and Science University, Beaverton, OR, USA
J Bacteriol 194:1697-707. 2012..The results strongly argue for a model in which a single Spx monomer engages RNAP to generate an active transcriptional complex...- Spx-RNA polymerase interaction and global transcriptional control during oxidative stressPeter Zuber
Department of Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health and Science University, Beaverton, Oregon 97006, USA
J Bacteriol 186:1911-8. 2004 - Activation of transcription initiation by Spx: formation of transcription complex and identification of a Cis-acting element required for transcriptional activationDindo Y Reyes
Department of Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health and Science University, 20000 NW Walker Rd, Beaverton, OR 97006, USA
Mol Microbiol 69:765-79. 2008..These data are consistent with a model that Spx activation requires contact between the Spx/RNAP complex and upstream promoter DNA, which allows Spx-induced engagement of the sigma and large subunits with the core promoter... 
Management of oxidative stress in BacillusPeter Zuber
Department of Science and Engineering, School of Medicine, Oregon Health and Science University, Beaverton, Oregon 97006, USA
Annu Rev Microbiol 63:575-97. 2009..An important unknown is the nature and influence of the low-molecular-weight thiols that mediate the buffering of the redox environment...- Phenotype enhancement screen of a regulatory spx mutant unveils a role for the ytpQ gene in the control of iron homeostasisPeter Zuber
Division of Environmental and Biomolecular Systems, Institute of Environmental Health, Oregon Health and Science University, Beaverton, Oregon, United States of America
PLoS ONE 6:e25066. 2011..In vitro transcription analysis indicated that Spx stimulates transcription from the ytpPQR operon within which the ytpQ gene resides. The work uncovers a link between Spx and control of iron homeostasis... - Regulation of quinone detoxification by the thiol stress sensing DUF24/MarR-like repressor, YodB in Bacillus subtilisMontira Leelakriangsak
Department of Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health and Science University, Beaverton, OR, USA
Mol Microbiol 67:1108-24. 2008..Thus, both azoreductases that are controlled by different regulatory mechanisms have common functions in quinone and azo-compound reduction to protect cells against the thiol reactivity of electrophiles... 
Redox-sensitive transcriptional control by a thiol/disulphide switch in the global regulator, SpxShunji Nakano
Department of Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health and Science University, Beaverton, OR 97006, USA
Mol Microbiol 55:498-510. 2005..The mechanism of Spx-dependent transcriptional activation is unique in that it does not involve initial Spx-DNA interaction...- Regulation of respiratory genes by ResD-ResE signal transduction system in Bacillus subtilisHao Geng
Department of Environmental System, Oregon Health and Science University, Beaverton, Oregon, USA
Methods Enzymol 422:448-64. 2007..ResD, after undergoing ResE-mediated phosphorylation, interacts with the C-terminal domain of the alpha subunit of RNA polymerase to activate transcription initiation at ResDE-controlled promoters... - Mutational analysis of the Bacillus subtilis RNA polymerase alpha C-terminal domain supports the interference model of Spx-dependent repressionYing Zhang
EBS/OGI School of Science and Engineering, OHSU, 20000 NW Walker Rd, Beaverton, OR 97006, USA
J Bacteriol 188:4300-11. 2006..Evidence is also presented that oxidized Spx, while enhancing interference of activator-RNAP interaction, is not essential for negative control... - Dual negative control of spx transcription initiation from the P3 promoter by repressors PerR and YodB in Bacillus subtilisMontira Leelakriangsak
OGI School of Science and Engineering, Oregon Health and Science University, Beaverton, OR 97229, USA
J Bacteriol 189:1736-44. 2007..The study has uncovered a mechanism of dual negative control that relates to the oxidative stress response of gram-positive bacteria... - Transcription from the P3 promoter of the Bacillus subtilis spx gene is induced in response to disulfide stressMontira Leelakriangsak
Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health and Science University, Beaverton, OR 97006, USA
J Bacteriol 189:1727-35. 2007..The data indicate that spx transcription is under negative transcriptional control that is reversed when disulfide stress is encountered... - Spx-dependent global transcriptional control is induced by thiol-specific oxidative stress in Bacillus subtilisShunji Nakano
Department of Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health and Science University, Beaverton, OR 97006, USA
Proc Natl Acad Sci U S A 100:13603-8. 2003.... - Control of gene expression in Gram-positive bacteria: extensions of and departures from enteric paradigmsPeter Zuber
Biochemistry and Molecular Biology, OGI School of Science and Engineering, Oregon Health and Science University, Beaverton, OR 97006, USA
Front Biosci 7:d1857-66. 2002..The accompanying reviews highlight the diversity and novel aspects of genetic control in Gram-positive bacteria, with descriptions of quorum-sensing systems, transcriptional control, and RNA processing mechanisms... - The global regulator Spx functions in the control of organosulfur metabolism in Bacillus subtilisSoon-Yong Choi
OGI School of Science and Engineering, Oregon Health and Science University, Beaverton, OR 97006, USA
J Bacteriol 188:5741-51. 2006..These experiments have uncovered additional roles for Spx in the control of gene expression during unperturbed, steady-state growth... - Sulfate-dependent repression of genes that function in organosulfur metabolism in Bacillus subtilis requires SpxKyle N Erwin
Department of Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health and Science University, 20000 NW Walker Rd, Beaverton, OR 97006, USA
J Bacteriol 187:4042-9. 2005..This work uncovers a new role for Spx in the control of sulfur metabolism in a gram-positive bacterium under nonstressful growth conditions... - Requirement of the zinc-binding domain of ClpX for Spx proteolysis in Bacillus subtilis and effects of disulfide stress on ClpXP activityYing Zhang
Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health and Science University, 20000 NW Walker Rd, Beaverton, OR 97006, USA
J Bacteriol 189:7669-80. 2007..The results are consistent with the hypothesis that inhibition of ClpXP by disulfide stress is due to structural changes to the N-terminal ZBD of ClpX... - Multiple pathways of Spx (YjbD) proteolysis in Bacillus subtilisShunji Nakano
Department of Biochemistry and Molecular Biology, OGI School of Science and Engineering, Oregon Health and Science University, Beaverton, Oregon 97006 8921, USA
J Bacteriol 184:3664-70. 2002..Spx probably is degraded by ClpCP under as yet unidentified conditions. This study suggests that the level of Spx is tightly controlled by two different ClpP proteases... - Promoter recognition by a complex of Spx and the C-terminal domain of the RNA polymerase alpha subunitMichiko M Nakano
Department of Science and Engineering, School of Medicine, Oregon Health and Science University, Beaverton, Oregon, USA
PLoS ONE 5:e8664. 2010..Spx activates trxA (thioredoxin) and trxB (thioredoxin reductase) in response to thiol stress, and bears an N-terminal C10XXC13 redox disulfide center that is oxidized in active Spx... - The YjbH protein of Bacillus subtilis enhances ClpXP-catalyzed proteolysis of SpxSaurabh K Garg
Division of Environmental and Biomolecular Systems, Department of Science and Engineering, School of Medicine, Oregon Health and Science University, Beaverton, Oregon 97006, USA
J Bacteriol 191:1268-77. 2009..In conclusion, YjbH is proposed to be an adaptor for ClpXP-catalyzed Spx degradation, and a model of YjbH redox control involving Zn dissociation is presented... - Spx (YjbD), a negative effector of competence in Bacillus subtilis, enhances ClpC-MecA-ComK interactionMichiko M Nakano
Department of Biochemistry and Molecular Biology, OGI School of Science and Engineering, Oregon Health and Science University, Beaverton, OR 97006 8921, USA
Mol Microbiol 44:1341-9. 2002..Spx is a highly conserved protein among Gram-positive bacteria, in which it may function closely with the protease adaptor protein, MecA... - A regulatory protein that interferes with activator-stimulated transcription in bacteriaShunji Nakano
Department of Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health and Science University, Beaverton, OR 97006, USA
Proc Natl Acad Sci U S A 100:4233-8. 2003..Spx represents a class of transcriptional regulators that inhibit activator-stimulated transcription by interaction with alpha... - YjbH-enhanced proteolysis of Spx by ClpXP in Bacillus subtilis is inhibited by the small protein YirB (YuzO)Sushma Kommineni
Department of Science and Engineering, Division of Environmental and Biomolecular Systems, Oregon Health and Science University, 20000 NW Walker Rd, Beaverton, OR 97006, USA
J Bacteriol 193:2133-40. 2011..The activity of YirB is similar to that of the antiadaptor proteins that were previously shown to reduce proteolysis of a specific ClpXP substrate by interacting with a substrate-binding protein...
Research Grants
- SRF OPERON--REGULATION AND ROLE IN GENETIC COMPETENCEPeter Zuber; Fiscal Year: 2003..These studies will further understanding of the functional links between stress-induced proteins and the regulation of prokaryotic cellular differentiation. ..
- Transcriptional Control by the Global Regulator SpxPeter Zuber; Fiscal Year: 2007..The effects of oxidants on Zn release and C1pXP activity will be determined in vitro. ..
- Transcriptional Control by the Global Regulator SpxPETER A ZUBER; Fiscal Year: 2010..The regulatory protein Spx controls important components of the bacterial response to oxidative attack, and the study of its mechanism of action will uncover targets for neutralizing infectious microorganisms. ..