Don Wolf

Summary

Affiliation: Oregon Health and Science University
Country: USA

Publications

  1. pmc Derivation and characterization of monkey embryonic stem cells
    K Y Francis Pau
    Primate Embryonic Stem Cell Program, Division of Reproductive Science, Oregon National Primate Research Center, Oregon Health and Science University West Campus, Beaverton, Oregon, USA
    Reprod Biol Endocrinol 2:41. 2004
  2. pmc Gene targeting in adult rhesus macaque fibroblasts
    Daniel T Meehan
    Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, 985805 Nebraska Medical Center, Omaha, NE 68198 5805, USA
    BMC Biotechnol 8:31. 2008
  3. ncbi request reprint Nuclear transfer in the rhesus monkey: practical and basic implications
    D P Wolf
    Division of Reproductive Sciences, Oregon Regional Primate Research Center, Beaverton, Oregon 97006, USA
    Biol Reprod 60:199-204. 1999
  4. ncbi request reprint The non-human primate oocyte and embryo as a model for women, or is it vice versa?
    D P Wolf
    Oregon Health and Science University, Oregon National Primate Research Center, Beaverton, OR 97006, USA
    Theriogenology 69:31-6. 2008
  5. doi request reprint Nonhuman primate embryonic stem cells: an underutilized resource
    Don P Wolf
    Oregon Health and Sciences University, Oregon National Primate Research Center, 505 NW 185th Avenue, Beaverton, OR 97006, USA
    Regen Med 3:129-31. 2008
  6. pmc Assisted reproductive technologies in rhesus macaques
    Don P Wolf
    Division of Reproductive Sciences, Oregon National Primate Research Center Beaverton, Oregon, USA
    Reprod Biol Endocrinol 2:37. 2004
  7. pmc ARTs in action in non-human primates: introduction to workshop proceedings
    Don P Wolf
    Division of Reproductive Sciences, Oregon National Primate Research Center Beaverton, Oregon, USA
    Reprod Biol Endocrinol 2:30. 2004
  8. ncbi request reprint Progress with nonhuman primate embryonic stem cells
    Don P Wolf
    Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon 97006, USA
    Biol Reprod 71:1766-71. 2004
  9. pmc An opinion on regulating the assisted reproductive technologies
    Don P Wolf
    Oregon National Primate Research Center, Oregon Health and Sciences University, 505 NW 185th, Beaverton, Oregon 97006, USA
    J Assist Reprod Genet 20:290-2. 2003
  10. doi request reprint Artificial insemination and the assisted reproductive technologies in non-human primates
    D P Wolf
    Oregon Health and Sciences University, Oregon National Primate Research Center, Beaverton, OR 97006, USA
    Theriogenology 71:123-9. 2009

Research Grants

  1. NUCLEAR TRANSFER IN RHESUS MONKEYS
    Don Wolf; Fiscal Year: 2000
  2. PROPAGATION OF MONKEY MODELS OF HUMAN DISEASE
    Shoukhrat Mitalipov; Fiscal Year: 2005
  3. Genetically Modified Rhesus Monkeys
    Don Wolf; Fiscal Year: 2004
  4. PROPAGATION OF MONKEY MODELS OF HUMAN DISEASE
    Don Wolf; Fiscal Year: 2004
  5. The ARTs in Action in Nonhuman Primates
    Don Wolf; Fiscal Year: 2003
  6. Genetically Modified Rhesus Monkeys
    Don Wolf; Fiscal Year: 2003
  7. PROPAGATION OF MONKEY MODELS OF HUMAN DISEASE
    Don Wolf; Fiscal Year: 2003
  8. PROPAGATION OF MONKEY MODELS OF HUMAN DISEASE
    Don Wolf; Fiscal Year: 2002
  9. Genetically Modified Rhesus Monkeys
    Don Wolf; Fiscal Year: 2002
  10. EVALUATION OF THE POTENCY OF RHESUS MONKEY STERM CELLS
    Don Wolf; Fiscal Year: 2001

Collaborators

Detail Information

Publications38

  1. pmc Derivation and characterization of monkey embryonic stem cells
    K Y Francis Pau
    Primate Embryonic Stem Cell Program, Division of Reproductive Science, Oregon National Primate Research Center, Oregon Health and Science University West Campus, Beaverton, Oregon, USA
    Reprod Biol Endocrinol 2:41. 2004
    ..The promises and limitations of primate ES cell-based therapy are also discussed...
  2. pmc Gene targeting in adult rhesus macaque fibroblasts
    Daniel T Meehan
    Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, 985805 Nebraska Medical Center, Omaha, NE 68198 5805, USA
    BMC Biotechnol 8:31. 2008
    ..Our goal was to establish the protocols necessary to achieve gene targeting in primary culture of adult rhesus macaque fibroblasts as a first step in creating nonhuman primate models of genetic disease using nuclear transfer technology...
  3. ncbi request reprint Nuclear transfer in the rhesus monkey: practical and basic implications
    D P Wolf
    Division of Reproductive Sciences, Oregon Regional Primate Research Center, Beaverton, Oregon 97006, USA
    Biol Reprod 60:199-204. 1999
    ..Ongoing research focused on the production of clonally derived rhesus monkeys using fetal fibroblasts and embryonic stem cells as the source of donor nuclei will be reviewed...
  4. ncbi request reprint The non-human primate oocyte and embryo as a model for women, or is it vice versa?
    D P Wolf
    Oregon Health and Science University, Oregon National Primate Research Center, Beaverton, OR 97006, USA
    Theriogenology 69:31-6. 2008
    ....
  5. doi request reprint Nonhuman primate embryonic stem cells: an underutilized resource
    Don P Wolf
    Oregon Health and Sciences University, Oregon National Primate Research Center, 505 NW 185th Avenue, Beaverton, OR 97006, USA
    Regen Med 3:129-31. 2008
  6. pmc Assisted reproductive technologies in rhesus macaques
    Don P Wolf
    Division of Reproductive Sciences, Oregon National Primate Research Center Beaverton, Oregon, USA
    Reprod Biol Endocrinol 2:37. 2004
    ..Finally, efforts to produce genetically-identical monkeys by nuclear transfer have been briefly summarized...
  7. pmc ARTs in action in non-human primates: introduction to workshop proceedings
    Don P Wolf
    Division of Reproductive Sciences, Oregon National Primate Research Center Beaverton, Oregon, USA
    Reprod Biol Endocrinol 2:30. 2004
  8. ncbi request reprint Progress with nonhuman primate embryonic stem cells
    Don P Wolf
    Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon 97006, USA
    Biol Reprod 71:1766-71. 2004
    ..Finally, safety, efficiency, and feasibility issues relating to the transplantation of ES-derived cells are considered...
  9. pmc An opinion on regulating the assisted reproductive technologies
    Don P Wolf
    Oregon National Primate Research Center, Oregon Health and Sciences University, 505 NW 185th, Beaverton, Oregon 97006, USA
    J Assist Reprod Genet 20:290-2. 2003
  10. doi request reprint Artificial insemination and the assisted reproductive technologies in non-human primates
    D P Wolf
    Oregon Health and Sciences University, Oregon National Primate Research Center, Beaverton, OR 97006, USA
    Theriogenology 71:123-9. 2009
    ....
  11. ncbi request reprint Nuclear transfer and cloning
    D P Wolf
    Oregon Regional Primate Research Center, Division of Reproductive Sciences, 505 NW 185th Avenue, Beaverton, OR 97006, USA
    Curr Womens Health Rep 1:164-7. 2001
    ..However, support is extended for human therapeutic cloning involving the derivation and use of embryonic stem cells to treat human disease...
  12. ncbi request reprint Nuclear transfer technology in mammalian cloning
    D P Wolf
    Division of Reproductive Sciences, Oregon Regional Primate Research Center, Beaverton, OR 97006, USA
    Arch Med Res 32:609-13. 2001
    ..Here we will focus on the potential uses of reproductive cloning along with recent activities in the field and a discussion concerning current interests in human reproductive and therapeutic cloning...
  13. pmc Discovery of a novel imprinted gene by transcriptional analysis of parthenogenetic embryonic stem cells
    Hathaitip Sritanaudomchai
    Oregon National Primate Research Center, Beaverton, OR 97006, USA
    Hum Reprod 25:1927-41. 2010
    ....
  14. pmc Heterozygous embryonic stem cell lines derived from nonhuman primate parthenotes
    Vikas Dighe
    Oregon National Primate Research Center, Beaverton, Oregon 97006, USA
    Stem Cells 26:756-66. 2008
    ....
  15. pmc Epigenetic reprogramming by somatic cell nuclear transfer in primates
    Michelle Sparman
    Division of Reproductive Sciences, Oregon National Primate Research Center, School of Medicine, Oregon Health and Science University, Beaverton, Oregon, USA
    Stem Cells 27:1255-64. 2009
    ..Moreover, imprinted gene expression, methylation, telomere length, and X-inactivation analyses were consistent with accurate and extensive epigenetic reprogramming of somatic cells by oocyte-specific factors...
  16. pmc CDX2 in the formation of the trophectoderm lineage in primate embryos
    Hathaitip Sritanaudomchai
    Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR, USA
    Dev Biol 335:179-87. 2009
    ..These results provide evidence that CDX2 plays an essential role in functional TE formation during primate embryonic development...
  17. pmc Human embryonic stem cells derived by somatic cell nuclear transfer
    Masahito Tachibana
    Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 NW 185th Avenue, Beaverton, OR 97006, USA
    Cell 153:1228-38. 2013
    ..Gene expression and differentiation profiles in human NT-ESCs were similar to embryo-derived ESCs, suggesting efficient reprogramming of somatic cells to a pluripotent state...
  18. pmc Totipotency, pluripotency and nuclear reprogramming
    Shoukhrat Mitalipov
    Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 N W 185th Avenue, Beaverton, Oregon, 97006, USA
    Adv Biochem Eng Biotechnol 114:185-99. 2009
    ..In this review, we summarize two major approaches to reprogramming: (1) somatic cell nuclear transfer and (2) direct reprogramming using genetic manipulations...
  19. ncbi request reprint Coculture of monkey ovarian tissue increases survival after vitrification and slow-rate freezing
    Richard R Yeoman
    Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Sciences University, 505 185th Avenue, Beaverton, OR 97006, USA
    Fertil Steril 83:1248-54. 2005
    ..To assess whether coculture of monkey ovarian tissue after low-temperature storage enhances follicular viability. To assess a novel method of vitrifying ovarian tissue...
  20. ncbi request reprint Low temperature storage of rhesus monkey spermatozoa and fertility evaluation by intracytoplasmic injection
    Richard R Yeoman
    Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 185th Avenue, Beaverton, Oregon, OR 97006, USA
    Theriogenology 63:2356-71. 2005
    ....
  21. pmc Directed differentiation of rhesus monkey ES cells into pancreatic cell phenotypes
    Linda B Lester
    Department of Medicine, Oregon Health and Sciences University, Portland, OR, USA
    Reprod Biol Endocrinol 2:42. 2004
    ..Using this GFP detection system, we will enrich our population of insulin producing rES derived cells and track these cells post-transplantation in the non-human primate model...
  22. pmc Current progress with primate embryonic stem cells
    James A Byrne
    Division of Reproductive Sciences, Oregon National Primate Research Center, Beaverton, OR, USA
    Curr Stem Cell Res Ther 1:127-38. 2006
    ....
  23. ncbi request reprint Monozygotic twinning in rhesus monkeys by manipulation of in vitro-derived embryos
    Shoukhrat M Mitalipov
    Oregon Regional Primate Research Center, Oregon Health and Science University, Beaverton, OR 97006, USA
    Biol Reprod 66:1449-55. 2002
    ..Neither pregnancy resulted in term birth of monozygotic twins, but our results are sufficiently encouraging to justify a large-scale twinning trial in the rhesus macaque...
  24. ncbi request reprint Rhesus monkey embryos produced by nuclear transfer from embryonic blastomeres or somatic cells
    Shoukhrat M Mitalipov
    Oregon Regional Primate Research Center, Beaverton, OR 97006, USA
    Biol Reprod 66:1367-73. 2002
    ..Future efforts will focus on optimizing the production of somatic cell NT embryos that develop in high efficiency to the blastocyst stage in vitro...
  25. ncbi request reprint Methylation status of imprinting centers for H19/IGF2 and SNURF/SNRPN in primate embryonic stem cells
    Shoukhrat Mitalipov
    Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 NW 185th Ave, Beaverton, OR 97006, USA
    Stem Cells 25:581-8. 2007
    ..Our results demonstrate abnormal hypermethylation within the IGF2/H19 IC in all analyzed ESC lines, whereas the SNURF/SNRPN IC was differentially methylated, consistent with monoallelic expression...
  26. ncbi request reprint Differentiation of monkey embryonic stem cells into neural lineages
    Hung Chih Kuo
    Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton 97006, USA
    Biol Reprod 68:1727-35. 2003
    ....
  27. ncbi request reprint Evaluation of the vervet (Clorocebus aethiops) as a model for the assisted reproductive technologies
    Michelle L Sparman
    Division of Reproductive Sciences, Oregon National Primate Research Center, Beaverton, OR 97006, USA
    Am J Primatol 69:917-29. 2007
    ..These results emphasize the need to establish species-specific ovarian stimulation protocols and semen collection techniques if vervets are to be considered for basic and applied (ARTs) research on primate gametes or embryos...
  28. ncbi request reprint Serotonin neurons derived from rhesus monkey embryonic stem cells: similarities to CNS serotonin neurons
    Ugur Salli
    Division of Reproductive Sciences, Oregon National Primate Research Center, Beaverton 97006, USA
    Exp Neurol 188:351-64. 2004
    ....
  29. ncbi request reprint Oct-4 expression in pluripotent cells of the rhesus monkey
    Shoukhrat M Mitalipov
    Oregon National Primate Research Center, Beaverton, Oregon 97006, USA
    Biol Reprod 69:1785-92. 2003
    ..Finally, the observed lack or abnormal expression of Oct-4 in monkey nuclear transfer embryos suggests inadequate nuclear reprogramming...
  30. ncbi request reprint Isolation and characterization of novel rhesus monkey embryonic stem cell lines
    Shoukhrat Mitalipov
    Division of Reproductive Sciences, Oregon National Primate Research Center, 505 NW 185th Avenue, Beaverton, Oregon 97006, USA
    Stem Cells 24:2177-86. 2006
    ..We also describe initial efforts to compare the pluripotency of ESC lines by expression profiling, chimeric embryo formation, and in vitro-directed differentiation into endodermal, mesodermal, and ectodermal lineages...
  31. ncbi request reprint Nuclear transfer in nonhuman primates
    Shoukhrat M Mitalipov
    Oregon National Primate Research Center, Beaverton, USA
    Methods Mol Biol 348:151-68. 2006
    ..In this chapter, we describe detailed protocols for rhesus macaque embryonic cell NT, including oocyte and embryo production, micromanipulation, and embryo transfer in nonhuman primates...
  32. ncbi request reprint Transcriptional profiling of rhesus monkey embryonic stem cells
    James A Byrne
    Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon 97006, USA
    Biol Reprod 75:908-15. 2006
    ..These five mammalian genes are strongly expressed in rhesus monkey, mouse, and human ESCs, albeit only in the undifferentiated state, and represent the core key mammalian stemness factors...
  33. ncbi request reprint Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells
    Deepika Rajesh
    Department of Surgery, University of Wisconsin Medical School, K4 760 Clinical Science Center, 600 Highland Avenue, Madison, Wisconsin 53792 7375, USA
    Stem Cells 25:490-9. 2007
    ..Although rESCs have the potential for hematopoietic differentiation, they exhibit a pause at the hemangioblast stage of hematopoietic development in culture conditions developed for hESCs...
  34. ncbi request reprint Challenges of primate embryonic stem cell research
    Barry D Bavister
    Department of Biological Sciences, University of New Orleans, Louisiana 70148 2960, USA
    Cloning Stem Cells 7:82-94. 2005
    ..In contrast, ES cell research using only human cell lines is likely to be incomplete, which could hinder research progress, and delay or diminish the effective application of ES cell technology to the treatment of human diseases...
  35. ncbi request reprint Aberrant genomic imprinting in rhesus monkey embryonic stem cells
    Akihisa Fujimoto
    Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Japan
    Stem Cells 24:595-603. 2006
    ..In differentiated ESC progeny, these expression patterns were maintained. The implications of aberrant imprinted gene expression to ESC differentiation in vitro and on ESC-derived cell function in vivo after transplantation are unknown...
  36. ncbi request reprint Transplantable neural progenitor populations derived from rhesus monkey embryonic stem cells
    Tianqing Li
    Department of Reproduction and Development, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 Jiaochang Donglu, Kunming, Yunnan, 650223, China
    Stem Cells 23:1295-303. 2005
    ....
  37. pmc Trophoblast cell activation by trophinin ligation is implicated in human embryo implantation
    Kazuhiro Sugihara
    Cancer Research Center and Inflammation and Infectious Disease Center, Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037
    Proc Natl Acad Sci U S A 104:3799-804. 2007
    ..These results suggest that trophinin-mediated cell adhesion functions as a molecular switch for trophectoderm activation in human embryo implantation...
  38. pmc Methods in stem cell research
    Shoukhrat Mitalipov
    Methods 45:99-100. 2008

Research Grants15

  1. NUCLEAR TRANSFER IN RHESUS MONKEYS
    Don Wolf; Fiscal Year: 2000
    ..Also, the simplicity of separating IVF-produced embryos at early cleavage stages with the transfer of individual blastomeres or groups of blastomeres to surrogate zonae offers a low technology method of cloning that will be evaluated. ..
  2. PROPAGATION OF MONKEY MODELS OF HUMAN DISEASE
    Shoukhrat Mitalipov; Fiscal Year: 2005
    ..The applicants will select the optimal approach to twinning in year 1 and produce 5 sets of identical twins per year during years 2-5 of the study. ..
  3. Genetically Modified Rhesus Monkeys
    Don Wolf; Fiscal Year: 2004
    ..Such animals should provide a resource for the study of human neurogenetic diseases and serve as pre-clinical models for new experimental treatments including gene and stem cell based therapies. ..
  4. PROPAGATION OF MONKEY MODELS OF HUMAN DISEASE
    Don Wolf; Fiscal Year: 2004
    ..The applicants will select the optimal approach to twinning in year 1 and produce 5 sets of identical twins per year during years 2-5 of the study. ..
  5. The ARTs in Action in Nonhuman Primates
    Don Wolf; Fiscal Year: 2003
    ..This arrangement will permit attendees to attend both meetings and should encourage participation by research trainees and fellows. ..
  6. Genetically Modified Rhesus Monkeys
    Don Wolf; Fiscal Year: 2003
    ..Such animals should provide a resource for the study of human neurogenetic diseases and serve as pre-clinical models for new experimental treatments including gene and stem cell based therapies. ..
  7. PROPAGATION OF MONKEY MODELS OF HUMAN DISEASE
    Don Wolf; Fiscal Year: 2003
    ..The applicants will select the optimal approach to twinning in year 1 and produce 5 sets of identical twins per year during years 2-5 of the study. ..
  8. PROPAGATION OF MONKEY MODELS OF HUMAN DISEASE
    Don Wolf; Fiscal Year: 2002
    ..The applicants will select the optimal approach to twinning in year 1 and produce 5 sets of identical twins per year during years 2-5 of the study. ..
  9. Genetically Modified Rhesus Monkeys
    Don Wolf; Fiscal Year: 2002
    ..Such animals should provide a resource for the study of human neurogenetic diseases and serve as pre-clinical models for new experimental treatments including gene and stem cell based therapies. ..
  10. EVALUATION OF THE POTENCY OF RHESUS MONKEY STERM CELLS
    Don Wolf; Fiscal Year: 2001
    ..abstract_text> ..
  11. NUCLEAR TRANSFER IN RHESUS MONKEYS
    Don Wolf; Fiscal Year: 2001
    ..Also, the simplicity of separating IVF-produced embryos at early cleavage stages with the transfer of individual blastomeres or groups of blastomeres to surrogate zonae offers a low technology method of cloning that will be evaluated. ..
  12. PROPAGATION OF MONKEY MODELS OF HUMAN DISEASE
    Don Wolf; Fiscal Year: 2001
    ..The applicants will select the optimal approach to twinning in year 1 and produce 5 sets of identical twins per year during years 2-5 of the study. ..
  13. EVALUATION OF THE POTENCY OF RHESUS MONKEY STERM CELLS
    Don Wolf; Fiscal Year: 2000
    ..abstract_text> ..
  14. NUCLEAR TRANSFER IN RHESUS MONKEYS
    Don Wolf; Fiscal Year: 1999
    ..Also, the simplicity of separating IVF-produced embryos at early cleavage stages with the transfer of individual blastomeres or groups of blastomeres to surrogate zonae offers a low technology method of cloning that will be evaluated. ..
  15. Genetically Modified Rhesus Monkeys
    Don Wolf; Fiscal Year: 2005
    ..Such animals should provide a resource for the study of human neurogenetic diseases and serve as pre-clinical models for new experimental treatments including gene and stem cell based therapies. ..