Research Topics
Species | Don WolfSummaryAffiliation: Oregon Health and Science University Country: USA Publications
Research Grants
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Detail Information
Publications
Derivation and characterization of monkey embryonic stem cellsK Y Francis Pau
Primate Embryonic Stem Cell Program, Division of Reproductive Science, Oregon National Primate Research Center, Oregon Health and Science University West Campus, Beaverton, Oregon, USA
Reprod Biol Endocrinol 2:41. 2004..The promises and limitations of primate ES cell-based therapy are also discussed...
Gene targeting in adult rhesus macaque fibroblastsDaniel T Meehan
Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, 985805 Nebraska Medical Center, Omaha, NE 68198 5805, USA
BMC Biotechnol 8:31. 2008..Our goal was to establish the protocols necessary to achieve gene targeting in primary culture of adult rhesus macaque fibroblasts as a first step in creating nonhuman primate models of genetic disease using nuclear transfer technology...- Assisted reproductive technologies in rhesus macaquesDon P Wolf
Division of Reproductive Sciences, Oregon National Primate Research Center Beaverton, Oregon, USA
Reprod Biol Endocrinol 2:37. 2004..Finally, efforts to produce genetically-identical monkeys by nuclear transfer have been briefly summarized... - ARTs in action in non-human primates: introduction to workshop proceedingsDon P Wolf
Division of Reproductive Sciences, Oregon National Primate Research Center Beaverton, Oregon, USA
Reprod Biol Endocrinol 2:30. 2004 
Progress with nonhuman primate embryonic stem cellsDon P Wolf
Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon 97006, USA
Biol Reprod 71:1766-71. 2004..Finally, safety, efficiency, and feasibility issues relating to the transplantation of ES-derived cells are considered...- An opinion on regulating the assisted reproductive technologiesDon P Wolf
Oregon National Primate Research Center, Oregon Health and Sciences University, 505 NW 185th, Beaverton, Oregon 97006, USA
J Assist Reprod Genet 20:290-2. 2003 - The non-human primate oocyte and embryo as a model for women, or is it vice versa?D P Wolf
Oregon Health and Science University, Oregon National Primate Research Center, Beaverton, OR 97006, USA
Theriogenology 69:31-6. 2008.... - Nonhuman primate embryonic stem cells: an underutilized resourceDon P Wolf
Oregon Health and Sciences University, Oregon National Primate Research Center, 505 NW 185th Avenue, Beaverton, OR 97006, USA
Regen Med 3:129-31. 2008 - Nuclear transfer and cloningD P Wolf
Oregon Regional Primate Research Center, Division of Reproductive Sciences, 505 NW 185th Avenue, Beaverton, OR 97006, USA
Curr Womens Health Rep 1:164-7. 2001..However, support is extended for human therapeutic cloning involving the derivation and use of embryonic stem cells to treat human disease... 
Artificial insemination and the assisted reproductive technologies in non-human primatesD P Wolf
Oregon Health and Sciences University, Oregon National Primate Research Center, Beaverton, OR 97006, USA
Theriogenology 71:123-9. 2009....- Nuclear transfer technology in mammalian cloningD P Wolf
Division of Reproductive Sciences, Oregon Regional Primate Research Center, Beaverton, OR 97006, USA
Arch Med Res 32:609-13. 2001..Here we will focus on the potential uses of reproductive cloning along with recent activities in the field and a discussion concerning current interests in human reproductive and therapeutic cloning... - Nuclear transfer in the rhesus monkey: practical and basic implicationsD P Wolf
Division of Reproductive Sciences, Oregon Regional Primate Research Center, Beaverton, Oregon 97006, USA
Biol Reprod 60:199-204. 1999..Ongoing research focused on the production of clonally derived rhesus monkeys using fetal fibroblasts and embryonic stem cells as the source of donor nuclei will be reviewed... - Discovery of a novel imprinted gene by transcriptional analysis of parthenogenetic embryonic stem cellsHathaitip Sritanaudomchai
Oregon National Primate Research Center, Beaverton, OR 97006, USA
Hum Reprod 25:1927-41. 2010.... - Heterozygous embryonic stem cell lines derived from nonhuman primate parthenotesVikas Dighe
Oregon National Primate Research Center, Beaverton, Oregon 97006, USA
Stem Cells 26:756-66. 2008.... - CDX2 in the formation of the trophectoderm lineage in primate embryosHathaitip Sritanaudomchai
Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR, USA
Dev Biol 335:179-87. 2009..These results provide evidence that CDX2 plays an essential role in functional TE formation during primate embryonic development... - Epigenetic reprogramming by somatic cell nuclear transfer in primatesMichelle Sparman
Division of Reproductive Sciences, Oregon National Primate Research Center, School of Medicine, Oregon Health and Science University, Beaverton, Oregon, USA
Stem Cells 27:1255-64. 2009..Moreover, imprinted gene expression, methylation, telomere length, and X-inactivation analyses were consistent with accurate and extensive epigenetic reprogramming of somatic cells by oocyte-specific factors... - Monozygotic twinning in rhesus monkeys by manipulation of in vitro-derived embryosShoukhrat M Mitalipov
Oregon Regional Primate Research Center, Oregon Health and Science University, Beaverton, OR 97006, USA
Biol Reprod 66:1449-55. 2002..Neither pregnancy resulted in term birth of monozygotic twins, but our results are sufficiently encouraging to justify a large-scale twinning trial in the rhesus macaque... - Coculture of monkey ovarian tissue increases survival after vitrification and slow-rate freezingRichard R Yeoman
Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Sciences University, 505 185th Avenue, Beaverton, OR 97006, USA
Fertil Steril 83:1248-54. 2005..To assess whether coculture of monkey ovarian tissue after low-temperature storage enhances follicular viability. To assess a novel method of vitrifying ovarian tissue... - Low temperature storage of rhesus monkey spermatozoa and fertility evaluation by intracytoplasmic injectionRichard R Yeoman
Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 185th Avenue, Beaverton, Oregon, OR 97006, USA
Theriogenology 63:2356-71. 2005.... - Directed differentiation of rhesus monkey ES cells into pancreatic cell phenotypesLinda B Lester
Department of Medicine, Oregon Health and Sciences University, Portland, OR, USA
Reprod Biol Endocrinol 2:42. 2004..Using this GFP detection system, we will enrich our population of insulin producing rES derived cells and track these cells post-transplantation in the non-human primate model... - Current progress with primate embryonic stem cellsJames A Byrne
Division of Reproductive Sciences, Oregon National Primate Research Center, Beaverton, OR, USA
Curr Stem Cell Res Ther 1:127-38. 2006.... - Rhesus monkey embryos produced by nuclear transfer from embryonic blastomeres or somatic cellsShoukhrat M Mitalipov
Oregon Regional Primate Research Center, Beaverton, OR 97006, USA
Biol Reprod 66:1367-73. 2002..Future efforts will focus on optimizing the production of somatic cell NT embryos that develop in high efficiency to the blastocyst stage in vitro... - Methylation status of imprinting centers for H19/IGF2 and SNURF/SNRPN in primate embryonic stem cellsShoukhrat Mitalipov
Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 NW 185th Ave, Beaverton, OR 97006, USA
Stem Cells 25:581-8. 2007..Our results demonstrate abnormal hypermethylation within the IGF2/H19 IC in all analyzed ESC lines, whereas the SNURF/SNRPN IC was differentially methylated, consistent with monoallelic expression... - Differentiation of monkey embryonic stem cells into neural lineagesHung-Chih Kuo
Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton 97006, USA
Biol Reprod 68:1727-35. 2003.... - Isolation and characterization of novel rhesus monkey embryonic stem cell linesShoukhrat Mitalipov
Division of Reproductive Sciences, Oregon National Primate Research Center, 505 NW 185th Avenue, Beaverton, Oregon 97006, USA
Stem Cells 24:2177-86. 2006..We also describe initial efforts to compare the pluripotency of ESC lines by expression profiling, chimeric embryo formation, and in vitro-directed differentiation into endodermal, mesodermal, and ectodermal lineages... - Evaluation of the vervet (Clorocebus aethiops) as a model for the assisted reproductive technologiesMichelle L Sparman
Division of Reproductive Sciences, Oregon National Primate Research Center, Beaverton, OR 97006, USA
Am J Primatol 69:917-29. 2007..These results emphasize the need to establish species-specific ovarian stimulation protocols and semen collection techniques if vervets are to be considered for basic and applied (ARTs) research on primate gametes or embryos... - Oct-4 expression in pluripotent cells of the rhesus monkeyShoukhrat M Mitalipov
Oregon National Primate Research Center, Beaverton, Oregon 97006, USA
Biol Reprod 69:1785-92. 2003..Finally, the observed lack or abnormal expression of Oct-4 in monkey nuclear transfer embryos suggests inadequate nuclear reprogramming... - Serotonin neurons derived from rhesus monkey embryonic stem cells: similarities to CNS serotonin neuronsUgur Salli
Division of Reproductive Sciences, Oregon National Primate Research Center, Beaverton 97006, USA
Exp Neurol 188:351-64. 2004.... - Totipotency, pluripotency and nuclear reprogrammingShoukhrat Mitalipov
Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 N W 185th Avenue, Beaverton, Oregon, 97006, USA
Adv Biochem Eng Biotechnol 114:185-99. 2009..In this review, we summarize two major approaches to reprogramming: (1) somatic cell nuclear transfer and (2) direct reprogramming using genetic manipulations... - Nuclear transfer in nonhuman primatesShoukhrat M Mitalipov
Oregon National Primate Research Center, Beaverton, USA
Methods Mol Biol 348:151-68. 2006..In this chapter, we describe detailed protocols for rhesus macaque embryonic cell NT, including oocyte and embryo production, micromanipulation, and embryo transfer in nonhuman primates... - Transcriptional profiling of rhesus monkey embryonic stem cellsJames A Byrne
Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon 97006, USA
Biol Reprod 75:908-15. 2006..These five mammalian genes are strongly expressed in rhesus monkey, mouse, and human ESCs, albeit only in the undifferentiated state, and represent the core key mammalian stemness factors... - Aberrant genomic imprinting in rhesus monkey embryonic stem cellsAkihisa Fujimoto
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Japan
Stem Cells 24:595-603. 2006..In differentiated ESC progeny, these expression patterns were maintained. The implications of aberrant imprinted gene expression to ESC differentiation in vitro and on ESC-derived cell function in vivo after transplantation are unknown... - Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cellsDeepika Rajesh
Department of Surgery, University of Wisconsin Medical School, K4 760 Clinical Science Center, 600 Highland Avenue, Madison, Wisconsin 53792 7375, USA
Stem Cells 25:490-9. 2007..Although rESCs have the potential for hematopoietic differentiation, they exhibit a pause at the hemangioblast stage of hematopoietic development in culture conditions developed for hESCs... - Transplantable neural progenitor populations derived from rhesus monkey embryonic stem cellsTianqing Li
Department of Reproduction and Development, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 Jiaochang Donglu, Kunming, Yunnan, 650223, China
Stem Cells 23:1295-303. 2005.... - Challenges of primate embryonic stem cell researchBarry D Bavister
Department of Biological Sciences, University of New Orleans, Louisiana 70148 2960, USA
Cloning Stem Cells 7:82-94. 2005..In contrast, ES cell research using only human cell lines is likely to be incomplete, which could hinder research progress, and delay or diminish the effective application of ES cell technology to the treatment of human diseases... - Methods in stem cell researchShoukhrat Mitalipov
Methods 45:99-100. 2008 - Trophoblast cell activation by trophinin ligation is implicated in human embryo implantationKazuhiro Sugihara
Cancer Research Center and Inflammation and Infectious Disease Center, Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037
Proc Natl Acad Sci U S A 104:3799-804. 2007..These results suggest that trophinin-mediated cell adhesion functions as a molecular switch for trophectoderm activation in human embryo implantation...
Research Grants
- PROPAGATION OF MONKEY MODELS OF HUMAN DISEASEShoukhrat Mitalipov; Fiscal Year: 2005..The applicants will select the optimal approach to twinning in year 1 and produce 5 sets of identical twins per year during years 2-5 of the study. ..
- The ARTs in Action in Nonhuman PrimatesDon Wolf; Fiscal Year: 2003..This arrangement will permit attendees to attend both meetings and should encourage participation by research trainees and fellows. ..
- EVALUATION OF THE POTENCY OF RHESUS MONKEY STERM CELLSDon Wolf; Fiscal Year: 2001..abstract_text> ..
- NUCLEAR TRANSFER IN RHESUS MONKEYSDon Wolf; Fiscal Year: 2001..Also, the simplicity of separating IVF-produced embryos at early cleavage stages with the transfer of individual blastomeres or groups of blastomeres to surrogate zonae offers a low technology method of cloning that will be evaluated. ..
- Genetically Modified Rhesus MonkeysDon Wolf; Fiscal Year: 2005..Such animals should provide a resource for the study of human neurogenetic diseases and serve as pre-clinical models for new experimental treatments including gene and stem cell based therapies. ..