Research Topics
Genomes and Genes
| Gary ThomasSummaryAffiliation: Oregon Health and Science University Country: USA Publications
Research Grants
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Detail Information
Publications
Furin at the cutting edge: from protein traffic to embryogenesis and diseaseGary Thomas
Vollum Institute, 3181 SW Sam Jackson Park Road, Portland, Oregon 97239, USA
Nat Rev Mol Cell Biol 3:753-66. 2002..This review summarizes various features of furin--its structural and enzymatic properties, intracellular localization, trafficking, substrates, and roles in vivo...- Small molecule inhibition of HIV-1-induced MHC-I down-regulation identifies a temporally regulated switch in Nef actionJimmy D Dikeakos
Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA
Mol Biol Cell 21:3279-92. 2010..Together, these studies resolve the seemingly controversial models that describe Nef-induced MHC-I down-regulation and provide new insights into the mechanism of Nef action... - HIV-1 Nef binds PACS-2 to assemble a multikinase cascade that triggers major histocompatibility complex class I (MHC-I) down-regulation: analysis using short interfering RNA and knock-out miceKatelyn M Atkins
Vollum Institute, Portland, Oregon 97239, USA
J Biol Chem 283:11772-84. 2008..Together, these results demonstrate PACS-2 is required for Nef action and sorting of itinerant membrane cargo in the TGN/endosomal system... - An interdomain binding site on HIV-1 Nef interacts with PACS-1 and PACS-2 on endosomes to down-regulate MHC-IJimmy D Dikeakos
Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA
Mol Biol Cell 23:2184-97. 2012..Our results provide insight into the molecular basis of Nef action and suggest new strategies to combat HIV-1... - A PACS-1, GGA3 and CK2 complex regulates CI-MPR traffickingGregory K Scott
Vollum Institute, Oregon Health and Science University, Oregon Health Sciences University, Portland, 97239, USA
EMBO J 25:4423-35. 2006..Our results identify a CK2-controlled cascade regulating hydrolase trafficking and sorting of itinerant proteins in the TGN/endosomal system... - PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosisThomas Simmen
Vollum Institute, Portland, OR 97239 3098, USA
EMBO J 24:717-29. 2005..Together, our results identify PACS-2 as a novel sorting protein that links the ER-mitochondria axis to ER homeostasis and the control of cell fate, and provide new insights into Bid action... 
HIV-1 Nef assembles a Src family kinase-ZAP-70/Syk-PI3K cascade to downregulate cell-surface MHC-IChien Hui Hung
Vollum Institute, 3181 Southwest Sam Jackson Park Road, Portland, OR 97239, USA
Cell Host Microbe 1:121-33. 2007..The discovery of this Nef-SFK-ZAP-70/Syk-PI3K signaling pathway explains the hierarchal role of the Nef motifs in effecting immunoevasion...- Akt and 14-3-3 control a PACS-2 homeostatic switch that integrates membrane traffic with TRAIL-induced apoptosisJoseph E Aslan
Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA
Mol Cell 34:497-509. 2009..Together, these studies identify the phosphorylation state of PACS-2 Ser437 as a molecular switch that integrates cellular homeostasis with TRAIL-induced apoptosis... - The phosphorylation state of an autoregulatory domain controls PACS-1-directed protein trafficGregory K Scott
Vollum Institute, L 474, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
EMBO J 22:6234-44. 2003..These results suggest that coordinated signaling events regulate transport within the TGN/endosomal system through the phosphorylation state of both cargo and the sorting machinery... - Propeptides are sufficient to regulate organelle-specific pH-dependent activation of furin and proprotein convertase 1/3Stephanie L Dillon
Department of Biochemistry and Molecular Biology, Oregon Health and Science University, 3181 Southwest Sam Jackson Park Road, Portland, OR 97229, USA
J Mol Biol 423:47-62. 2012.... - Cleavages within the prodomain direct intracellular trafficking and degradation of mature bone morphogenetic protein-4Catherine Degnin
Department of Biochemistry and Molecular Biology, Oregon Health and Science University, School of Medicine, Portland, OR 97239-3098, USA
Mol Biol Cell 15:5012-20. 2004..Based on these results, we propose a model for how cleavage at the upstream site regulates the activity and signaling range of mature BMP-4 after it has been released from the prodomain... - Role of PACS-1 in trafficking of human cytomegalovirus glycoprotein B and virus productionColin M Crump
Vollum Institute, Oregon Health and Science University, Portland, Oregon 97239, USA
J Virol 77:11105-13. 2003.... 
Insights from bacterial subtilases into the mechanisms of intramolecular chaperone-mediated activation of furinUjwal Shinde
Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, OR 97229, USA
Methods Mol Biol 768:59-106. 2011....- At the crossroads of homoeostasis and disease: roles of the PACS proteins in membrane traffic and apoptosisRobert T Youker
Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
Biochem J 421:1-15. 2009.... - HIV-1 Nef downregulates MHC-I by a PACS-1- and PI3K-regulated ARF6 endocytic pathwayAnastassia D Blagoveshchenskaya
Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
Cell 111:853-66. 2002..These data provide new insights into the cellular basis of HIV-1 immunoevasion... - Death by committee: organellar trafficking and communication in apoptosisJoseph E Aslan
Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA
Traffic 10:1390-404. 2009.... - Kaposi sarcoma herpesvirus K5 removes CD31/PECAM from endothelial cellsMandana Mansouri
Vaccine and Gene Therapy Institute and Vollum Institute, Oregon Health and Science University, Portland, OR, USA
Blood 108:1932-40. 2006..K5-mediated degradation of CD31 is likely to affect EC function in KS tumors... - The ordered and compartment-specfific autoproteolytic removal of the furin intramolecular chaperone is required for enzyme activationEric D Anderson
Vollum Institute, Portland, Oregon 97201, USA
J Biol Chem 277:12879-90. 2002.... - Sustained phosphorylation of Bid is a marker for resistance to Fas-induced apoptosis during chronic liver diseasesArndt Vogel
Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, Oregon, USA
Gastroenterology 130:104-19. 2006..To clarify whether stress-induced apoptosis resistance is a general feature of chronic liver diseases, an animal model of chronic cholestasis was examined... - Retrieval of human cytomegalovirus glycoprotein B from cell surface is not required for virus envelopment in astrocytoma cellsMichael A Jarvis
Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201, USA
J Virol 76:5147-55. 2002..These observations indicate that, although endocytosis is involved in the trafficking of gB to the site of glycoprotein accumulation in the TGN, endocytosis of gB is not required for the production of infectious HCMV... - Inhibition of furin-mediated processing results in suppression of astrocytoma cell growth and invasivenessJavier Mercapide
Department of Pathology, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
Clin Cancer Res 8:1740-6. 2002..Because furin inhibition inhibits both invasiveness and cell growth in astrocytoma, furin should be considered a promising target for glioblastoma therapy... - Synthetic small molecule furin inhibitors derived from 2,5-dideoxystreptamineGuan Sheng Jiao
Departments of Chemistry, PanThera Biopharma LLC, 99 193 Aiea Heights Drive, Suite 136, Aiea, HI 96701, USA
Proc Natl Acad Sci U S A 103:19707-12. 2006.... - Proprotein convertase furin interacts with and cleaves pro-ADAMTS4 (Aggrecanase-1) in the trans-Golgi networkPing Wang
Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA
J Biol Chem 279:15434-40. 2004..These results indicate that the activation mechanism for ADAMTS4 can be targeted for therapeutical intervention against this enzyme... - Profibrillin-1 maturation by human dermal fibroblasts: proteolytic processing and molecular chaperonesDebra D Wallis
Department of Internal Medicine, University of Texas-Houston Medical School, Houston, Texas 77030, USA
J Cell Biochem 90:641-52. 2003..These studies delineate the secretion and proteolytic processing of profibrillin-1, and identify the proteins that interact with profibrillin-1 in the secretory pathway... - The PHD/LAP-domain protein M153R of myxomavirus is a ubiquitin ligase that induces the rapid internalization and lysosomal destruction of CD4Mandana Mansouri
Vaccine and Gene Therapy Institute, Oregon Health and Science University, Portland 97201, USA
J Virol 77:1427-40. 2003..Since a similar mechanism was recently proposed for KSHV K5, it seems that members of the unrelated families of gamma-2 herpesviruses and poxviruses share a common immune evasion mechanism that targets host cell immune receptors... - Identification of a pH sensor in the furin propeptide that regulates enzyme activationSylvain F Feliciangeli
Institut de Pharmacologie Moleculaire et Cellulaire, Valbonne, France
J Biol Chem 281:16108-16. 2006..Together, our data explain the pH-regulated activation of furin and how this His-dependent regulatory mechanism is a model for other proteins...
Research Grants
- Role of HCMV Glycoprotein in Viral BiogenesisGary Thomas; Fiscal Year: 2005..Together, these studies will increase our knowledge of the cell biology of HCMV gB and provide insight into the molecular mechanisms of viral assembly that will enable development of novel therapeutics to treat viral disease. ..
- REGULATION OF PROTEIN TRAFFIC IN NEUROENDOCRINE CELLSGary Thomas; Fiscal Year: 2005..abstract_text> ..
- Role of PACS-1 in HIV-1 ImmunoevasionGary Thomas; Fiscal Year: 2004..Together, these studies will increase our knowledge of the biochemical and cellular basis of HIV-1's ability to evade the immune surveillance system, as well as provide new insights into pharmacological strategies to block this process. ..
- Role of PACS proteins in polycystin-2 trafficking and ADPKDGary Thomas; Fiscal Year: 2010....
- Program in Molecular and Cellular BiosciencesGary Thomas; Fiscal Year: 2007..D. students to form cross-discipline collaborations during their training and to gain an appreciation of the health-relatedness of the basic science research that they undertake. ..
- Role of PACS-1 in HIV-1 ImmunoevasionGary Thomas; Fiscal Year: 2007..Elucidating how this Nef-induced signaling pathway is used by HIV-1 will provide better understanding of the biochemical basis underlying HIV-1 pathogenesis and will identify new targets to combat this deadly virus. ..
- CELL TYPE-SPECIFIC PROCESSING OF OPIOID PEPTIDESGary Thomas; Fiscal Year: 1993..Cell type-specific maturation, activation and modulation of PC2 and PC3 in vivo will be sought...
- REGULATION OF PROTEIN TRAFFIC IN NEUROENDOCRINE CELLSGary Thomas; Fiscal Year: 2001..In specific aim 4, the role of the first identified phosphorylation-dependent furin-cd binding protein, 45.1.1, in the trafficking of furin in neuroendocrine cells will be determined. ..
- CELL-TYPE SPECIFIC PROCESSING OF OPIOID PRECURSORSGary Thomas; Fiscal Year: 1992....
- Role of PACS proteins in polycystin-2 trafficking and ADPKDGary Thomas; Fiscal Year: 2010....