Gary Thomas

Summary

Affiliation: Oregon Health and Science University
Country: USA

Publications

  1. pmc Furin at the cutting edge: from protein traffic to embryogenesis and disease
    Gary Thomas
    Vollum Institute, 3181 SW Sam Jackson Park Road, Portland, Oregon 97239, USA
    Nat Rev Mol Cell Biol 3:753-66. 2002
  2. pmc Small molecule inhibition of HIV-1-induced MHC-I down-regulation identifies a temporally regulated switch in Nef action
    Jimmy D Dikeakos
    Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA
    Mol Biol Cell 21:3279-92. 2010
  3. pmc HIV-1 Nef binds PACS-2 to assemble a multikinase cascade that triggers major histocompatibility complex class I (MHC-I) down-regulation: analysis using short interfering RNA and knock-out mice
    Katelyn M Atkins
    Vollum Institute, Portland, Oregon 97239, USA
    J Biol Chem 283:11772-84. 2008
  4. pmc An interdomain binding site on HIV-1 Nef interacts with PACS-1 and PACS-2 on endosomes to down-regulate MHC-I
    Jimmy D Dikeakos
    Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA
    Mol Biol Cell 23:2184-97. 2012
  5. pmc A PACS-1, GGA3 and CK2 complex regulates CI-MPR trafficking
    Gregory K Scott
    Vollum Institute, Oregon Health and Science University, Oregon Health Sciences University, Portland, 97239, USA
    EMBO J 25:4423-35. 2006
  6. pmc PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis
    Thomas Simmen
    Vollum Institute, Portland, OR 97239 3098, USA
    EMBO J 24:717-29. 2005
  7. pmc Akt and 14-3-3 control a PACS-2 homeostatic switch that integrates membrane traffic with TRAIL-induced apoptosis
    Joseph E Aslan
    Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA
    Mol Cell 34:497-509. 2009
  8. ncbi request reprint HIV-1 Nef assembles a Src family kinase-ZAP-70/Syk-PI3K cascade to downregulate cell-surface MHC-I
    Chien Hui Hung
    Vollum Institute, 3181 Southwest Sam Jackson Park Road, Portland, OR 97239, USA
    Cell Host Microbe 1:121-33. 2007
  9. pmc The phosphorylation state of an autoregulatory domain controls PACS-1-directed protein traffic
    Gregory K Scott
    Vollum Institute, L 474, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    EMBO J 22:6234-44. 2003
  10. pmc The mechanism by which a propeptide-encoded pH sensor regulates spatiotemporal activation of furin
    Danielle M Williamson
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland Oregon 97239, USA
    J Biol Chem 288:19154-65. 2013

Collaborators

Detail Information

Publications27

  1. pmc Furin at the cutting edge: from protein traffic to embryogenesis and disease
    Gary Thomas
    Vollum Institute, 3181 SW Sam Jackson Park Road, Portland, Oregon 97239, USA
    Nat Rev Mol Cell Biol 3:753-66. 2002
    ..This review summarizes various features of furin--its structural and enzymatic properties, intracellular localization, trafficking, substrates, and roles in vivo...
  2. pmc Small molecule inhibition of HIV-1-induced MHC-I down-regulation identifies a temporally regulated switch in Nef action
    Jimmy D Dikeakos
    Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA
    Mol Biol Cell 21:3279-92. 2010
    ..Together, these studies resolve the seemingly controversial models that describe Nef-induced MHC-I down-regulation and provide new insights into the mechanism of Nef action...
  3. pmc HIV-1 Nef binds PACS-2 to assemble a multikinase cascade that triggers major histocompatibility complex class I (MHC-I) down-regulation: analysis using short interfering RNA and knock-out mice
    Katelyn M Atkins
    Vollum Institute, Portland, Oregon 97239, USA
    J Biol Chem 283:11772-84. 2008
    ..Together, these results demonstrate PACS-2 is required for Nef action and sorting of itinerant membrane cargo in the TGN/endosomal system...
  4. pmc An interdomain binding site on HIV-1 Nef interacts with PACS-1 and PACS-2 on endosomes to down-regulate MHC-I
    Jimmy D Dikeakos
    Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA
    Mol Biol Cell 23:2184-97. 2012
    ..Our results provide insight into the molecular basis of Nef action and suggest new strategies to combat HIV-1...
  5. pmc A PACS-1, GGA3 and CK2 complex regulates CI-MPR trafficking
    Gregory K Scott
    Vollum Institute, Oregon Health and Science University, Oregon Health Sciences University, Portland, 97239, USA
    EMBO J 25:4423-35. 2006
    ..Our results identify a CK2-controlled cascade regulating hydrolase trafficking and sorting of itinerant proteins in the TGN/endosomal system...
  6. pmc PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis
    Thomas Simmen
    Vollum Institute, Portland, OR 97239 3098, USA
    EMBO J 24:717-29. 2005
    ..Together, our results identify PACS-2 as a novel sorting protein that links the ER-mitochondria axis to ER homeostasis and the control of cell fate, and provide new insights into Bid action...
  7. pmc Akt and 14-3-3 control a PACS-2 homeostatic switch that integrates membrane traffic with TRAIL-induced apoptosis
    Joseph E Aslan
    Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA
    Mol Cell 34:497-509. 2009
    ..Together, these studies identify the phosphorylation state of PACS-2 Ser437 as a molecular switch that integrates cellular homeostasis with TRAIL-induced apoptosis...
  8. ncbi request reprint HIV-1 Nef assembles a Src family kinase-ZAP-70/Syk-PI3K cascade to downregulate cell-surface MHC-I
    Chien Hui Hung
    Vollum Institute, 3181 Southwest Sam Jackson Park Road, Portland, OR 97239, USA
    Cell Host Microbe 1:121-33. 2007
    ..The discovery of this Nef-SFK-ZAP-70/Syk-PI3K signaling pathway explains the hierarchal role of the Nef motifs in effecting immunoevasion...
  9. pmc The phosphorylation state of an autoregulatory domain controls PACS-1-directed protein traffic
    Gregory K Scott
    Vollum Institute, L 474, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    EMBO J 22:6234-44. 2003
    ..These results suggest that coordinated signaling events regulate transport within the TGN/endosomal system through the phosphorylation state of both cargo and the sorting machinery...
  10. pmc The mechanism by which a propeptide-encoded pH sensor regulates spatiotemporal activation of furin
    Danielle M Williamson
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland Oregon 97239, USA
    J Biol Chem 288:19154-65. 2013
    ....
  11. pmc Role of PACS-1 in trafficking of human cytomegalovirus glycoprotein B and virus production
    Colin M Crump
    Vollum Institute, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Virol 77:11105-13. 2003
    ....
  12. pmc Propeptides are sufficient to regulate organelle-specific pH-dependent activation of furin and proprotein convertase 1/3
    Stephanie L Dillon
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, 3181 Southwest Sam Jackson Park Road, Portland, OR 97229, USA
    J Mol Biol 423:47-62. 2012
    ....
  13. pmc Cleavages within the prodomain direct intracellular trafficking and degradation of mature bone morphogenetic protein-4
    Catherine Degnin
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, School of Medicine, Portland, OR 97239 3098, USA
    Mol Biol Cell 15:5012-20. 2004
    ..Based on these results, we propose a model for how cleavage at the upstream site regulates the activity and signaling range of mature BMP-4 after it has been released from the prodomain...
  14. doi request reprint Insights from bacterial subtilases into the mechanisms of intramolecular chaperone-mediated activation of furin
    Ujwal Shinde
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, OR 97229, USA
    Methods Mol Biol 768:59-106. 2011
    ....
  15. ncbi request reprint HIV-1 Nef downregulates MHC-I by a PACS-1- and PI3K-regulated ARF6 endocytic pathway
    Anastassia D Blagoveshchenskaya
    Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    Cell 111:853-66. 2002
    ..These data provide new insights into the cellular basis of HIV-1 immunoevasion...
  16. pmc Kaposi sarcoma herpesvirus K5 removes CD31/PECAM from endothelial cells
    Mandana Mansouri
    Vaccine and Gene Therapy Institute and Vollum Institute, Oregon Health and Science University, Portland, OR, USA
    Blood 108:1932-40. 2006
    ..K5-mediated degradation of CD31 is likely to affect EC function in KS tumors...
  17. doi request reprint At the crossroads of homoeostasis and disease: roles of the PACS proteins in membrane traffic and apoptosis
    Robert T Youker
    Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    Biochem J 421:1-15. 2009
    ....
  18. pmc The PHD/LAP-domain protein M153R of myxomavirus is a ubiquitin ligase that induces the rapid internalization and lysosomal destruction of CD4
    Mandana Mansouri
    Vaccine and Gene Therapy Institute, Oregon Health and Science University, Portland 97201, USA
    J Virol 77:1427-40. 2003
    ..Since a similar mechanism was recently proposed for KSHV K5, it seems that members of the unrelated families of gamma-2 herpesviruses and poxviruses share a common immune evasion mechanism that targets host cell immune receptors...
  19. pmc Death by committee: organellar trafficking and communication in apoptosis
    Joseph E Aslan
    Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA
    Traffic 10:1390-404. 2009
    ....
  20. pmc The ordered and compartment-specfific autoproteolytic removal of the furin intramolecular chaperone is required for enzyme activation
    Eric D Anderson
    Vollum Institute, Portland, Oregon 97201, USA
    J Biol Chem 277:12879-90. 2002
    ....
  21. pmc Sustained phosphorylation of Bid is a marker for resistance to Fas-induced apoptosis during chronic liver diseases
    Arndt Vogel
    Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, Oregon, USA
    Gastroenterology 130:104-19. 2006
    ..To clarify whether stress-induced apoptosis resistance is a general feature of chronic liver diseases, an animal model of chronic cholestasis was examined...
  22. pmc Retrieval of human cytomegalovirus glycoprotein B from cell surface is not required for virus envelopment in astrocytoma cells
    Michael A Jarvis
    Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201, USA
    J Virol 76:5147-55. 2002
    ..These observations indicate that, although endocytosis is involved in the trafficking of gB to the site of glycoprotein accumulation in the TGN, endocytosis of gB is not required for the production of infectious HCMV...
  23. ncbi request reprint Inhibition of furin-mediated processing results in suppression of astrocytoma cell growth and invasiveness
    Javier Mercapide
    Department of Pathology, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    Clin Cancer Res 8:1740-6. 2002
    ..In the present study inhibition of furin was achieved by gene transfer of alpha(1)-antitrypsin Portland (PDX) cDNA...
  24. pmc Synthetic small molecule furin inhibitors derived from 2,5-dideoxystreptamine
    Guan Sheng Jiao
    Departments of Chemistry, PanThera Biopharma LLC, 99 193 Aiea Heights Drive, Suite 136, Aiea, HI 96701, USA
    Proc Natl Acad Sci U S A 103:19707-12. 2006
    ....
  25. ncbi request reprint Proprotein convertase furin interacts with and cleaves pro-ADAMTS4 (Aggrecanase-1) in the trans-Golgi network
    Ping Wang
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 279:15434-40. 2004
    ..These results indicate that the activation mechanism for ADAMTS4 can be targeted for therapeutical intervention against this enzyme...
  26. pmc Profibrillin-1 maturation by human dermal fibroblasts: proteolytic processing and molecular chaperones
    Debra D Wallis
    Department of Internal Medicine, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Cell Biochem 90:641-52. 2003
    ..These studies delineate the secretion and proteolytic processing of profibrillin-1, and identify the proteins that interact with profibrillin-1 in the secretory pathway...
  27. ncbi request reprint Identification of a pH sensor in the furin propeptide that regulates enzyme activation
    Sylvain F Feliciangeli
    Institut de Pharmacologie Moleculaire et Cellulaire, Valbonne, France
    J Biol Chem 281:16108-16. 2006
    ..Together, our data explain the pH-regulated activation of furin and how this His-dependent regulatory mechanism is a model for other proteins...

Research Grants40

  1. Role of HCMV Glycoprotein in Viral Biogenesis
    Gary Thomas; Fiscal Year: 2001
    ..Together, these studies will increase our knowledge of the cell biology of HCMV gB and provide insight into the molecular mechanisms of viral assembly that will enable development of novel therapeutics to treat viral disease. ..
  2. Role of PACS-1 in HIV-1 Immunoevasion
    Gary Thomas; Fiscal Year: 2002
    ..Together, these studies will increase our knowledge of the biochemical and cellular basis of HIV-1's ability to evade the immune surveillance system, as well as provide new insights into pharmacological strategies to block this process. ..
  3. REGULATION OF PROTEIN TRAFFIC IN NEUROENDOCRINE CELLS
    Gary Thomas; Fiscal Year: 2003
    ..abstract_text> ..
  4. Role of HCMV Glycoprotein in Viral Biogenesis
    Gary Thomas; Fiscal Year: 2004
    ..Together, these studies will increase our knowledge of the cell biology of HCMV gB and provide insight into the molecular mechanisms of viral assembly that will enable development of novel therapeutics to treat viral disease. ..
  5. Role of PACS-1 in HIV-1 Immunoevasion
    Gary Thomas; Fiscal Year: 2005
    ..Elucidating how this Nef-induced signaling pathway is used by HIV-1 will provide better understanding of the biochemical basis underlying HIV-1 pathogenesis and will identify new targets to combat this deadly virus. ..
  6. Program in Molecular and Cellular Biosciences
    Gary Thomas; Fiscal Year: 2007
    ..D. students to form cross-discipline collaborations during their training and to gain an appreciation of the health-relatedness of the basic science research that they undertake. ..
  7. Role of PACS proteins in polycystin-2 trafficking and ADPKD
    Gary Thomas; Fiscal Year: 2007
    ....
  8. Role of PACS proteins in polycystin-2 trafficking and ADPKD
    Gary Thomas; Fiscal Year: 2010
    ....
  9. CELL-TYPE SPECIFIC PROCESSING OF OPIOID PRECURSORS
    Gary Thomas; Fiscal Year: 1990
    ....
  10. REGULATION OF PROTEIN TRAFFIC IN NEUROENDOCRINE CELLS
    Gary Thomas; Fiscal Year: 1999
    ..In specific aim 4, the role of the first identified phosphorylation-dependent furin-cd binding protein, 45.1.1, in the trafficking of furin in neuroendocrine cells will be determined. ..
  11. REGULATION OF PROTEIN TRAFFIC IN NEUROENDOCRINE CELLS
    Gary Thomas; Fiscal Year: 2004
    ..abstract_text> ..
  12. Role of PACS-1 in HIV-1 Immunoevasion
    Gary Thomas; Fiscal Year: 2004
    ..Together, these studies will increase our knowledge of the biochemical and cellular basis of HIV-1's ability to evade the immune surveillance system, as well as provide new insights into pharmacological strategies to block this process. ..
  13. REGULATION OF PROTEIN TRAFFIC IN NEUROENDOCRINE CELLS
    Gary Thomas; Fiscal Year: 2005
    ..abstract_text> ..
  14. Role of PACS-1 in HIV-1 Immunoevasion
    Gary Thomas; Fiscal Year: 2006
    ..Elucidating how this Nef-induced signaling pathway is used by HIV-1 will provide better understanding of the biochemical basis underlying HIV-1 pathogenesis and will identify new targets to combat this deadly virus. ..
  15. Role of PACS proteins in polycystin-2 trafficking and ADPKD
    Gary Thomas; Fiscal Year: 2006
    ....
  16. Role of PACS-1 in HIV-1 Immunoevasion
    Gary Thomas; Fiscal Year: 2003
    ..Together, these studies will increase our knowledge of the biochemical and cellular basis of HIV-1's ability to evade the immune surveillance system, as well as provide new insights into pharmacological strategies to block this process. ..
  17. Role of PACS proteins in polycystin-2 trafficking and ADPKD
    Gary Thomas; Fiscal Year: 2010
    ....
  18. REGULATION OF PROTEIN TRAFFIC IN NEUROENDOCRINE CELLS
    Gary Thomas; Fiscal Year: 2001
    ..In specific aim 4, the role of the first identified phosphorylation-dependent furin-cd binding protein, 45.1.1, in the trafficking of furin in neuroendocrine cells will be determined. ..
  19. CELL-TYPE SPECIFIC PROCESSING OF OPIOID PRECURSORS
    Gary Thomas; Fiscal Year: 1992
    ....
  20. Role of HCMV Glycoprotein in Viral Biogenesis
    Gary Thomas; Fiscal Year: 2002
    ..Together, these studies will increase our knowledge of the cell biology of HCMV gB and provide insight into the molecular mechanisms of viral assembly that will enable development of novel therapeutics to treat viral disease. ..
  21. CELL TYPE-SPECIFIC PROCESSING OF OPIOID PEPTIDES
    Gary Thomas; Fiscal Year: 1993
    ..Cell type-specific maturation, activation and modulation of PC2 and PC3 in vivo will be sought...
  22. Role of PACS proteins in polycystin-2 trafficking and ADPKD
    Gary Thomas; Fiscal Year: 2009
    ....
  23. CELL-TYPE SPECIFIC PROCESSING OF OPIOID PRECURSORS
    Gary Thomas; Fiscal Year: 1991
    ....
  24. Role of PACS-1 in HIV-1 Immunoevasion
    Gary Thomas; Fiscal Year: 2007
    ..Elucidating how this Nef-induced signaling pathway is used by HIV-1 will provide better understanding of the biochemical basis underlying HIV-1 pathogenesis and will identify new targets to combat this deadly virus. ..
  25. REGULATION OF PROTEIN TRAFFIC IN NEUROENDOCRINE CELLS
    Gary Thomas; Fiscal Year: 2000
    ..In specific aim 4, the role of the first identified phosphorylation-dependent furin-cd binding protein, 45.1.1, in the trafficking of furin in neuroendocrine cells will be determined. ..
  26. Role of PACS-1 in HIV-1 Immunoevasion
    Gary Thomas; Fiscal Year: 2001
    ..Together, these studies will increase our knowledge of the biochemical and cellular basis of HIV-1's ability to evade the immune surveillance system, as well as provide new insights into pharmacological strategies to block this process. ..
  27. Role of HCMV Glycoprotein in Viral Biogenesis
    Gary Thomas; Fiscal Year: 2005
    ..Together, these studies will increase our knowledge of the cell biology of HCMV gB and provide insight into the molecular mechanisms of viral assembly that will enable development of novel therapeutics to treat viral disease. ..
  28. REGULATION OF PROTEIN TRAFFIC IN NEUROENDOCRINE CELLS
    Gary Thomas; Fiscal Year: 2002
    ..abstract_text> ..
  29. Role of HCMV Glycoprotein in Viral Biogenesis
    Gary Thomas; Fiscal Year: 2003
    ..Together, these studies will increase our knowledge of the cell biology of HCMV gB and provide insight into the molecular mechanisms of viral assembly that will enable development of novel therapeutics to treat viral disease. ..