Robert L Tanguay


Affiliation: Oregon State University
Country: USA


  1. Truong L, Mandrell D, Mandrell R, Simonich M, Tanguay R. A rapid throughput approach identifies cognitive deficits in adult zebrafish from developmental exposure to polybrominated flame retardants. Neurotoxicology. 2014;43:134-142 pubmed publisher
    ..Active avoidance response in zebrafish is an effective model of learning that, combined with automated shuttle box testing, will provide a highly efficient platform for evaluating persistent neurotoxic hazard from many chemicals. ..
  2. Roper C, Simonich S, Tanguay R. Development of a high-throughput in vivo screening platform for particulate matter exposures. Environ Pollut. 2018;235:993-1005 pubmed publisher
  3. Elie M, Choi J, Nkrumah Elie Y, Gonnerman G, Stevens J, Tanguay R. Metabolomic analysis to define and compare the effects of PAHs and oxygenated PAHs in developing zebrafish. Environ Res. 2015;140:502-10 pubmed publisher
    ..Our previous transcriptomic and genomic data were incorporated in this metabolomics study to provide a more comprehensive view of the relationship between PAH and oxy-PAH exposures on vertebrate development. ..
  4. Reif D, Truong L, Mandrell D, Marvel S, Zhang G, Tanguay R. High-throughput characterization of chemical-associated embryonic behavioral changes predicts teratogenic outcomes. Arch Toxicol. 2016;90:1459-70 pubmed publisher
    ..Therefore, this system can provide rapid characterization of chemical-elicited behavioral responses at an early developmental stage that are predictive of observable adverse effects later in life. ..
  5. Geier M, James Minick D, Truong L, Tilton S, Pande P, Anderson K, et al. Systematic developmental neurotoxicity assessment of a representative PAH Superfund mixture using zebrafish. Toxicol Appl Pharmacol. 2018;354:115-125 pubmed publisher
    ..Collectively, these results exemplify the utility of zebrafish to investigate the developmental and neurotoxicity of complex mixtures. ..
  6. Noyes P, Haggard D, Gonnerman G, Tanguay R. Advanced morphological - behavioral test platform reveals neurodevelopmental defects in embryonic zebrafish exposed to comprehensive suite of halogenated and organophosphate flame retardants. Toxicol Sci. 2015;145:177-95 pubmed publisher
    ..Taken together, evidence presented here indicates that zebrafish neurodevelopment is highly sensitive to many flame retardants currently in use and can be used to understand potential vulnerabilities to human health. ..
  7. Perkins A, Phillips J, Kerkvliet N, Tanguay R, Perdew G, Kolluri S, et al. A Structural Switch between Agonist and Antagonist Bound Conformations for a Ligand-Optimized Model of the Human Aryl Hydrocarbon Receptor Ligand Binding Domain. Biology (Basel). 2014;3:645-69 pubmed publisher
    ..This flexible segment of the AHR may act as a structural switch that determines the agonist or antagonist activity of a given AHR ligand. ..
  8. Bugel S, Wehmas L, La Du J, Tanguay R. Phenotype anchoring in zebrafish reveals a potential role for matrix metalloproteinases (MMPs) in tamoxifen's effects on skin epithelium. Toxicol Appl Pharmacol. 2016;296:31-41 pubmed publisher
    ..These studies help advance our understanding of tamoxifen's non-classical mode of action and implicate a possible role for MMPs in tissues such as skin. ..
  9. Bugel S, Tanguay R. Multidimensional chemobehavior analysis of flavonoids and neuroactive compounds in zebrafish. Toxicol Appl Pharmacol. 2018;344:23-34 pubmed publisher
    ..Overall, the integrative zebrafish platform provides a useful framework for comparatively evaluating high-content chemobehavioral data for sets of structurally- and mechanistically-related flavonoids and neuroactive compounds. ..

More Information


  1. Truong L, Simonich M, Tanguay R. Better, Faster, Cheaper: Getting the Most Out of High-Throughput Screening with Zebrafish. Methods Mol Biol. 2016;1473:89-98 pubmed publisher
    ..We include optimization and harmonization of dosing volume, exposure paradigms, chemical solubility, chorion status, experimental duration, endpoint definitions, and statistical analysis. ..
  2. Truong L, Gonnerman G, Simonich M, Tanguay R. Assessment of the developmental and neurotoxicity of the mosquito control larvicide, pyriproxyfen, using embryonic zebrafish. Environ Pollut. 2016;218:1089-1093 pubmed publisher
    ..Thus, zebrafish data suggest that the developmental toxicity of pyriproxyfen may not be limited to insects. ..
  3. Zhang G, Roell K, Truong L, Tanguay R, Reif D. A data-driven weighting scheme for multivariate phenotypic endpoints recapitulates zebrafish developmental cascades. Toxicol Appl Pharmacol. 2017;314:109-117 pubmed publisher
    ..Thus, wAggE offers a powerful approach for analysis of multivariate phenotypes that can reveal underlying etiological processes. ..
  4. Haggard D, Noyes P, Waters K, Tanguay R. Transcriptomic and phenotypic profiling in developing zebrafish exposed to thyroid hormone receptor agonists. Reprod Toxicol. 2018;77:80-93 pubmed publisher
    ..This unique signature has the potential to be used as a tiered in vivo HTS and may aid in identifying chemicals that interact with the thyroid hormone receptor. ..
  5. Chen J, Lei L, Tian L, Hou F, Roper C, Ge X, et al. Developmental and behavioral alterations in zebrafish embryonically exposed to valproic acid (VPA): An aquatic model for autism. Neurotoxicol Teratol. 2018;66:8-16 pubmed publisher
    ..Our study demonstrated that VPA exposure generates ASD-like phenotypes and behaviors, indicating that zebrafish is an alternative model to investigate underlying ASD mechanisms. ..
  6. Knecht A, Truong L, Simonich M, Tanguay R. Developmental benzo[a]pyrene (B[a]P) exposure impacts larval behavior and impairs adult learning in zebrafish. Neurotoxicol Teratol. 2017;59:27-34 pubmed publisher
    ..Developmentally exposed B[a]P zebrafish exhibited decreased learning and memory. Together this data demonstrates that developmental B[a]P exposure adversely impacts larval behavior, and learning in adult zebrafish. ..
  7. Chlebowski A, Tanguay R, Simonich S. Quantitation and prediction of sorptive losses during toxicity testing of polycyclic aromatic hydrocarbon (PAH) and nitrated PAH (NPAH) using polystyrene 96-well plates. Neurotoxicol Teratol. 2016;57:30-38 pubmed publisher
    ..This indicates the need to better understand the sorptive behavior of hydrophobic analytes to plastics, and to better account for sorptive losses during toxicity testing in polystyrene plates. ..
  8. Zhang G, Marvel S, Truong L, Tanguay R, Reif D. Aggregate entropy scoring for quantifying activity across endpoints with irregular correlation structure. Reprod Toxicol. 2016;62:92-9 pubmed publisher
    ..By testing AggE in a variety of high-dimensional real and simulated datasets, we have characterized its performance and suggested implementation parameters that can guide its application across a wide range of experimental scenarios. ..
  9. Garcia G, Noyes P, Tanguay R. Advancements in zebrafish applications for 21st century toxicology. Pharmacol Ther. 2016;161:11-21 pubmed publisher
    ..Breakthroughs in transgenic/reporter lines and methods for genetic manipulation, such as the CRISPR-Cas9 system, will be comprised of reports across diverse disciplines. ..
  10. Haggard D, Noyes P, Waters K, Tanguay R. Phenotypically anchored transcriptome profiling of developmental exposure to the antimicrobial agent, triclosan, reveals hepatotoxicity in embryonic zebrafish. Toxicol Appl Pharmacol. 2016;308:32-45 pubmed publisher
    ..We observed similarities in gene expression and hepatic steatosis assays; however, hit data for TCS were more concordant with the hypothesized CAR/PXR activity of TCS from rodent and human in vitro studies. ..
  11. Gerlach C, Das S, Volz D, Bisson W, Kolluri S, Tanguay R. Mono-substituted isopropylated triaryl phosphate, a major component of Firemaster 550, is an AHR agonist that exhibits AHR-independent cardiotoxicity in zebrafish. Aquat Toxicol. 2014;154:71-9 pubmed publisher
    ..Overall, these results suggest that, while mITP is an AHR agonist, mITP causes AHR-independent cardiotoxicity through a pathway that is also antagonized by CH223191. ..
  12. Beaver L, Nkrumah Elie Y, Truong L, Barton C, Knecht A, Gonnerman G, et al. Adverse effects of parental zinc deficiency on metal homeostasis and embryonic development in a zebrafish model. J Nutr Biochem. 2017;43:78-87 pubmed publisher
    ..These data should inform future studies regarding zinc deficiency and pregnancy and suggest that supplementation of zinc deficient mothers prior to pregnancy may be beneficial. ..
  13. Tanguay R. Challenges and Advances in Nanotoxicology. Nanomaterials (Basel). 2014;4:766-767 pubmed publisher
    ..Keeping modern toxicology abreast of this innovation to the point that it guides a safer nanotechnology presents an equally exciting and eminently worthwhile challenge. [...]. ..
  14. Geier M, Chlebowski A, Truong L, Massey Simonich S, Anderson K, Tanguay R. Comparative developmental toxicity of a comprehensive suite of polycyclic aromatic hydrocarbons. Arch Toxicol. 2018;92:571-586 pubmed publisher
    ..High-molecular weight PAHs were significantly more developmentally toxic than the low-molecular weight PAHs, and CYP1A expression was detected in five distinct tissues, including vasculature, liver, skin, neuromasts and yolk. ..
  15. Jang H, Pearce M, O Donnell E, Nguyen B, Truong L, Mueller M, et al. Identification of a Raloxifene Analog That Promotes AhR-Mediated Apoptosis in Cancer Cells. Biology (Basel). 2017;6: pubmed publisher
    ..We conclude that Y134 is a promising raloxifene analog for further optimization as an anti-cancer agent targeting the AhR. ..
  16. Goodale B, La Du J, Tilton S, Sullivan C, Bisson W, Waters K, et al. Ligand-Specific Transcriptional Mechanisms Underlie Aryl Hydrocarbon Receptor-Mediated Developmental Toxicity of Oxygenated PAHs. Toxicol Sci. 2015;147:397-411 pubmed publisher
    ..Identifying ligand-dependent AHR interactions and signaling pathways is essential for understanding toxicity of this class of environmentally relevant compounds. ..
  17. Truong L, Bugel S, Chlebowski A, Usenko C, Simonich M, Simonich S, et al. Optimizing multi-dimensional high throughput screening using zebrafish. Reprod Toxicol. 2016;65:139-147 pubmed publisher
    ..Adaptation of these better practices for zebrafish HTS should increase reproducibility across laboratories. ..
  18. Garcia G, Goodale B, Wiley M, La Du J, Hendrix D, Tanguay R. In Vivo Characterization of an AHR-Dependent Long Noncoding RNA Required for Proper Sox9b Expression. Mol Pharmacol. 2017;91:609-619 pubmed publisher
    ..Our results place slincR as an intermediate between AHR2 activation and the reduction of sox9b mRNA in the AHR2 signaling pathway. ..
  19. Knecht A, Truong L, Marvel S, Reif D, Garcia A, Lu C, et al. Transgenerational inheritance of neurobehavioral and physiological deficits from developmental exposure to benzo[a]pyrene in zebrafish. Toxicol Appl Pharmacol. 2017;329:148-157 pubmed publisher
    ..Exposure to benzo[a]pyrene during development resulted in transgenerational inheritance of neurobehavioral and physiological deficiencies. Indirect evidence suggested the potential for an AHR2-dependent epigenetic route. ..
  20. Truong L, Tanguay R. Evaluation of Embryotoxicity Using the Zebrafish Model. Methods Mol Biol. 2017;1641:325-333 pubmed publisher
    ..Numerous morphological, developmental, and behavioral endpoints can be evaluated noninvasively due to the transparent nature of the embryos. ..
  21. Franzosa J, Bugel S, Tal T, La Du J, Tilton S, Waters K, et al. Retinoic acid-dependent regulation of miR-19 expression elicits vertebrate axis defects. FASEB J. 2013;27:4866-76 pubmed publisher
    ..This highlights a previously unidentified role of miR-19 in facilitating vertebrate axis development via regulation of RA signaling. ..