Affiliation: Oregon State University
- Genomic and phenotypic characterization of in vitro-generated Chlamydia trachomatis recombinantsBrendan M Jeffrey
Molecular and Cellular Biology Program, Oregon State University, Corvallis, OR, USA
BMC Microbiol 13:142. 2013..These strains were used to explore the process of recombination in Chlamydia spp., including analysis of candidate recombination hotspots, and to correlate known C. trachomatis in vitro phenotypes with parental phenotypes and genotypes...
- Chlamydial development is blocked in host cells transfected with Chlamydophila caviae incADamir Alzhanov
Department of Microbiology, Oregon State University, Corvallis, OR 97331 3804 USA
BMC Microbiol 4:24. 2004..Little information exists regarding the interaction of Inc proteins with the eukaryotic cell. To examine these interactions, Vaccinia virus vectors and mammalian plasmid-based systems were used to express inc genes in mammalian cells...
- Cytokinesis is blocked in mammalian cells transfected with Chlamydia trachomatis gene CT223Damir T Alzhanov
Department of Microbiology, Oregon State University, Corvallis, OR 97331, USA
BMC Microbiol 9:2. 2009..The inc gene CT223 is one of a sequential set of orfs that encode or are predicted to encode Inc proteins. CT223p is localized to the inclusion membrane in all tested C. trachomatis serovars...
- Intrastrain and interstrain genetic variation within a paralogous gene family in Chlamydia pneumoniaeWasna Viratyosin
Department of Microbiology, Oregon State University, Corvallis, 97331 3804, USA
BMC Microbiol 2:38. 2002..Each member encodes a polypeptide with a hydrophobic domain characteristic of proteins localized to the inclusion membrane...
- Unraveling the basic biology and clinical significance of the chlamydial plasmidDaniel D Rockey
Department of Biomedical Sciences, Oregon State University College of Veterinary Medicine, Corvallis, OR 97331, USA
J Exp Med 208:2159-62. 2011..Collectively, these studies open new avenues of research into developing vaccines against trachoma and sexually transmitted chlamydial infections...
- Proteins in the chlamydial inclusion membraneDaniel D Rockey
Department of Microbiology, Oregon State University, Corvallis, OR 97331 3804, USA
Microbes Infect 4:333-40. 2002..Several proteins have recently been identified that are localized to the inclusion membrane. The following is a discussion of how inclusion membrane proteins might participate in the chlamydial developmental process...
- Tetracycline resistance in Chlamydia suis mediated by genomic islands inserted into the chlamydial inv-like geneJae Dugan
Department of Biomedical Sciences, Oregon State University, 106 Dryden Hall, Corvallis, OR 97331 4804, USA
Antimicrob Agents Chemother 48:3989-95. 2004..These genomic islands represent the first examples of horizontally acquired DNA integrated into a natural isolate of chlamydiae or within any other obligate intracellular bacterium...
- Diversity within inc genes of clinical Chlamydia trachomatis variant isolates that occupy non-fusogenic inclusionsDaniel D Rockey
Department of Microbiology, Oregon State University, Corvallis OR 97331 3804, USA
Microbiology 148:2497-505. 2002..trachomatis isolates. No change in the structure or the fusogenicity of the inclusions was associated with the presence or absence of CT223p...
- Host alpha-adducin is redistributed and localized to the inclusion membrane in chlamydia- and chlamydophila-infected cellsHencelyn G Chu
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331 4804, USA
Microbiology 154:3848-55. 2008..The demonstration of alpha-adducin and Raf-1 redistribution within cells infected by different chlamydiae provides novel opportunities for analysis of host-pathogen interactions in this system...
- Genome sequencing of recent clinical Chlamydia trachomatis strains identifies loci associated with tissue tropism and regions of apparent recombinationBrendan M Jeffrey
Department of Biomedical Sciences, Oregon State University, Corvallis, Oregon 97331 4804, USA
Infect Immun 78:2544-53. 2010..This work demonstrated that whole-genome sequencing along with comparative genomics is an effective approach for discovering variable loci in Chlamydia spp. that are associated with clinical presentation...
- Functional characterization of IScs605, an insertion element carried by tetracycline-resistant Chlamydia suisJae Dugan
Department of Biomedical Sciences, College of Veterinary Medicine and the Molecular and Cellular Biology Program, Oregon State University, Corvallis, OR 97331, USA
Microbiology 153:71-9. 2007..suis chromosome...
- Chlamydia vaccine candidates and tools for chlamydial antigen discoveryDaniel D Rockey
Associate Professor, College of Veterinary Medicine, Oregon State University, 211 Dryden Hall, Corvallis, OR 97331 4804, USA
Expert Rev Vaccines 8:1365-77. 2009..In this article, we will summarize progress in these areas with a focus on chlamydial vaccine antigen discovery, and discuss future directions towards the development of a safe and effective chlamydial vaccine...
- Clonal isolation of chlamydia-infected cells using flow cytometryDamir T Alzhanov
Department of Microbiology, College of Science, Oregon State University, Corvallis, OR 97331, USA
J Microbiol Methods 68:201-8. 2007..It is anticipated that FACS-based sorting of live chlamydia-infected cells will be a significant technical tool for the isolation of clonal populations of any chlamydial strain...
- Chlamydia trachomatis variant with nonfusing inclusions: growth dynamic and host-cell transcriptional responseMinsheng Xia
Division of Infectious Diseases, Department of Medicine, University of Washington, Seattle, USA
J Infect Dis 192:1229-36. 2005..The variant strain grew significantly more slowly and induced a modified host-cell transcriptional response, compared with the prototype strain...
- Development of secondary inclusions in cells infected by Chlamydia trachomatisRobert J Suchland
Division of Allergy and Infectious Diseases, School of Medicine, University of Washington, Seattle 98105, USA
Infect Immun 73:3954-62. 2005....
- PROTEINS IN THE CHLAMYDIAL INCLUSION MEMBRANEDANIEL ROCKEY; Fiscal Year: 2002..These different approaches will examine the unique biology of IncA/B/C as well as explore new technologies for investigating chlamydial interactions with mammalian cells. ..
- CHLAMYDIA TRACHOMATIS INCA MUTANTSDANIEL ROCKEY; Fiscal Year: 2005..Study of these natural mutants will lead to a better understanding of chlamydial growth, development and pathogenesis. ..
- Genetic transformation system for Chlamydia suisDANIEL ROCKEY; Fiscal Year: 2007..This will assist in the identification and analysis of novel vaccine candidates and to a clearer understanding of the mechanism of virulence in these important pathogens. ..