Shoukhrat Mitalipov

Summary

Affiliation: Oregon Health and Science University
Country: USA

Publications

  1. pmc Mitochondrial gene replacement in primate offspring and embryonic stem cells
    Masahito Tachibana
    Oregon National Primate Research Center, USA
    Nature 461:367-72. 2009
  2. pmc Clinical and ethical implications of mitochondrial gene transfer
    Shoukhrat Mitalipov
    Oregon National Primate Research Center, Oregon Health and Science University, 505 North West 185th Avenue, Beaverton, OR 97006, USA Electronic address
    Trends Endocrinol Metab 25:5-7. 2014
  3. pmc Systems biology discoveries using non-human primate pluripotent stem and germ cells: novel gene and genomic imprinting interactions as well as unique expression patterns
    Ahmi Ben-Yehudah
    Pittsburgh Development Center, 204 Craft Avenue, Pittsburgh, PA 15213, USA
    Stem Cell Res Ther 1:24. 2010
  4. pmc Gene targeting in adult rhesus macaque fibroblasts
    Daniel T Meehan
    Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, 985805 Nebraska Medical Center, Omaha, NE 68198 5805, USA
    BMC Biotechnol 8:31. 2008
  5. ncbi request reprint Methylation status of imprinting centers for H19/IGF2 and SNURF/SNRPN in primate embryonic stem cells
    Shoukhrat Mitalipov
    Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 NW 185th Ave, Beaverton, OR 97006, USA
    Stem Cells 25:581-8. 2007
  6. ncbi request reprint Reprogramming following somatic cell nuclear transfer in primates is dependent upon nuclear remodeling
    S M Mitalipov
    Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 NW 185th Avenue, Beaverton, OR 97006, USA
    Hum Reprod 22:2232-42. 2007
  7. ncbi request reprint Isolation and characterization of novel rhesus monkey embryonic stem cell lines
    Shoukhrat Mitalipov
    Division of Reproductive Sciences, Oregon National Primate Research Center, 505 NW 185th Avenue, Beaverton, Oregon 97006, USA
    Stem Cells 24:2177-86. 2006
  8. ncbi request reprint Genomic imprinting in primate embryos and embryonic stem cells
    Shoukhrat M Mitalipov
    Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 NW 185th Avenue, Beaverton, OR 97006, USA
    Reprod Fertil Dev 18:817-21. 2006
  9. pmc Human embryonic stem cells derived by somatic cell nuclear transfer
    Masahito Tachibana
    Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 NW 185th Avenue, Beaverton, OR 97006, USA
    Cell 153:1228-38. 2013
  10. pmc Epigenetic reprogramming by somatic cell nuclear transfer in primates
    Michelle Sparman
    Division of Reproductive Sciences, Oregon National Primate Research Center, School of Medicine, Oregon Health and Science University, Beaverton, Oregon, USA
    Stem Cells 27:1255-64. 2009

Research Grants

  1. Genetically Modified Rhesus Monkeys
    Shoukhrat Mitalipov; Fiscal Year: 2006
  2. Histocompatible Primate Embryonic Stem Cells
    Shoukhrat Mitalipov; Fiscal Year: 2009
  3. Altered Nuclear Transfer
    Shoukhrat M Mitalipov; Fiscal Year: 2010
  4. Mitochondrial Gene Therapy
    Shoukhrat M Mitalipov; Fiscal Year: 2010
  5. Histocompatible Primate Embryonic Stem Cells
    Shoukhrat M Mitalipov; Fiscal Year: 2010

Collaborators

Detail Information

Publications34

  1. pmc Mitochondrial gene replacement in primate offspring and embryonic stem cells
    Masahito Tachibana
    Oregon National Primate Research Center, USA
    Nature 461:367-72. 2009
    ..This approach may offer a reproductive option to prevent mtDNA disease transmission in affected families...
  2. pmc Clinical and ethical implications of mitochondrial gene transfer
    Shoukhrat Mitalipov
    Oregon National Primate Research Center, Oregon Health and Science University, 505 North West 185th Avenue, Beaverton, OR 97006, USA Electronic address
    Trends Endocrinol Metab 25:5-7. 2014
    ..Here we review the efficacy and safety of these approaches and their associated ethical and regulatory issues...
  3. pmc Systems biology discoveries using non-human primate pluripotent stem and germ cells: novel gene and genomic imprinting interactions as well as unique expression patterns
    Ahmi Ben-Yehudah
    Pittsburgh Development Center, 204 Craft Avenue, Pittsburgh, PA 15213, USA
    Stem Cell Res Ther 1:24. 2010
    ..In this review, we consider the mRNAs and novel genes with unique expression and imprinting patterns that were discovered using systems biology approaches with primate pluripotent stem and germ cells...
  4. pmc Gene targeting in adult rhesus macaque fibroblasts
    Daniel T Meehan
    Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, 985805 Nebraska Medical Center, Omaha, NE 68198 5805, USA
    BMC Biotechnol 8:31. 2008
    ..Our goal was to establish the protocols necessary to achieve gene targeting in primary culture of adult rhesus macaque fibroblasts as a first step in creating nonhuman primate models of genetic disease using nuclear transfer technology...
  5. ncbi request reprint Methylation status of imprinting centers for H19/IGF2 and SNURF/SNRPN in primate embryonic stem cells
    Shoukhrat Mitalipov
    Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 NW 185th Ave, Beaverton, OR 97006, USA
    Stem Cells 25:581-8. 2007
    ..Our results demonstrate abnormal hypermethylation within the IGF2/H19 IC in all analyzed ESC lines, whereas the SNURF/SNRPN IC was differentially methylated, consistent with monoallelic expression...
  6. ncbi request reprint Reprogramming following somatic cell nuclear transfer in primates is dependent upon nuclear remodeling
    S M Mitalipov
    Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 NW 185th Avenue, Beaverton, OR 97006, USA
    Hum Reprod 22:2232-42. 2007
    ..Given prior difficulties in SCNT in primates using conventional protocols, we hypothesized that the ability of cytoplasts to induce nuclear remodeling was instrumental in efficient reprogramming...
  7. ncbi request reprint Isolation and characterization of novel rhesus monkey embryonic stem cell lines
    Shoukhrat Mitalipov
    Division of Reproductive Sciences, Oregon National Primate Research Center, 505 NW 185th Avenue, Beaverton, Oregon 97006, USA
    Stem Cells 24:2177-86. 2006
    ..We also describe initial efforts to compare the pluripotency of ESC lines by expression profiling, chimeric embryo formation, and in vitro-directed differentiation into endodermal, mesodermal, and ectodermal lineages...
  8. ncbi request reprint Genomic imprinting in primate embryos and embryonic stem cells
    Shoukhrat M Mitalipov
    Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 NW 185th Avenue, Beaverton, OR 97006, USA
    Reprod Fertil Dev 18:817-21. 2006
    ..Cellular overproliferation and tumour formation resulting from tissue or cell transplantation are potential problems that must be addressed before clinical trials of ES cell-based therapy are initiated...
  9. pmc Human embryonic stem cells derived by somatic cell nuclear transfer
    Masahito Tachibana
    Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 NW 185th Avenue, Beaverton, OR 97006, USA
    Cell 153:1228-38. 2013
    ..Gene expression and differentiation profiles in human NT-ESCs were similar to embryo-derived ESCs, suggesting efficient reprogramming of somatic cells to a pluripotent state...
  10. pmc Epigenetic reprogramming by somatic cell nuclear transfer in primates
    Michelle Sparman
    Division of Reproductive Sciences, Oregon National Primate Research Center, School of Medicine, Oregon Health and Science University, Beaverton, Oregon, USA
    Stem Cells 27:1255-64. 2009
    ..Moreover, imprinted gene expression, methylation, telomere length, and X-inactivation analyses were consistent with accurate and extensive epigenetic reprogramming of somatic cells by oocyte-specific factors...
  11. pmc Towards germline gene therapy of inherited mitochondrial diseases
    Masahito Tachibana
    Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 NW 185th Avenue, Beaverton, Oregon 97006, USA
    Nature 493:627-31. 2013
    ..The mtDNA can be efficiently replaced in human oocytes. Although some ST oocytes displayed abnormal fertilization, remaining embryos were capable of developing to blastocysts and producing embryonic stem cells similar to controls...
  12. pmc Discovery of a novel imprinted gene by transcriptional analysis of parthenogenetic embryonic stem cells
    Hathaitip Sritanaudomchai
    Oregon National Primate Research Center, Beaverton, OR 97006, USA
    Hum Reprod 25:1927-41. 2010
    ....
  13. pmc CDX2 in the formation of the trophectoderm lineage in primate embryos
    Hathaitip Sritanaudomchai
    Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR, USA
    Dev Biol 335:179-87. 2009
    ..These results provide evidence that CDX2 plays an essential role in functional TE formation during primate embryonic development...
  14. pmc Nuclear reprogramming by interphase cytoplasm of two-cell mouse embryos
    Eunju Kang
    Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon 97006, USA
    Nature 509:101-4. 2014
    ..The ability to use interphase cytoplasm in SCNT could aid efforts to generate autologous human ES cells for regenerative applications, as donated or discarded embryos are more accessible than unfertilized MII oocytes. ..
  15. pmc Heterozygous embryonic stem cell lines derived from nonhuman primate parthenotes
    Vikas Dighe
    Oregon National Primate Research Center, Beaverton, Oregon 97006, USA
    Stem Cells 26:756-66. 2008
    ....
  16. pmc Rapid mitochondrial DNA segregation in primate preimplantation embryos precedes somatic and germline bottleneck
    Hyo Sang Lee
    Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR 97006, USA
    Cell Rep 1:506-15. 2012
    ....
  17. pmc Chromosome transfer in mature oocytes
    Masahito Tachibana
    Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA
    Fertil Steril 97:e16. 2012
    ..To demonstrate step-by-step micromanipulation procedures required for transfer of spindle-chromosomal complexes between mature oocytes...
  18. pmc Generation of chimeric rhesus monkeys
    Masahito Tachibana
    Oregon National Primate Research Center, Oregon Health and Science University, 505 N W 185 th Avenue, Beaverton, OR 97006, USA
    Cell 148:285-95. 2012
    ..These results provide insights into the species-specific nature of primate embryos and suggest that a chimera assay using pluripotent cells may not be feasible...
  19. pmc Chromosome transfer in mature oocytes
    Masahito Tachibana
    Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA
    Nat Protoc 5:1138-47. 2010
    ..Chromosome transfer between the cohorts of oocytes isolated from two females can be completed within 2 h...
  20. pmc Totipotency, pluripotency and nuclear reprogramming
    Shoukhrat Mitalipov
    Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 N W 185th Avenue, Beaverton, Oregon, 97006, USA
    Adv Biochem Eng Biotechnol 114:185-99. 2009
    ..In this review, we summarize two major approaches to reprogramming: (1) somatic cell nuclear transfer and (2) direct reprogramming using genetic manipulations...
  21. pmc Current progress with primate embryonic stem cells
    James A Byrne
    Division of Reproductive Sciences, Oregon National Primate Research Center, Beaverton, OR, USA
    Curr Stem Cell Res Ther 1:127-38. 2006
    ....
  22. pmc Concise review: parthenote stem cells for regenerative medicine: genetic, epigenetic, and developmental features
    Brittany Daughtry
    Departments of Cell and Developmental Biology and Molecular and Medical Genetics, and Program in Molecular and Cellular Biosciences, School of Medicine, and Divisions of Reproductive and Developmental Sciences, Oregon National Primate Research Center, and Reproductive Endocrinology, Department of Obstetrics and Gynecology, Oregon Health and Science University, Portland, Oregon, USA
    Stem Cells Transl Med 3:290-8. 2014
    ..In this study, we describe the biology and genetic nature of mammalian parthenogenesis and review potential advantages and limitations of pESCs for cell-based therapies. ..
  23. ncbi request reprint Low temperature storage of rhesus monkey spermatozoa and fertility evaluation by intracytoplasmic injection
    Richard R Yeoman
    Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 185th Avenue, Beaverton, Oregon, OR 97006, USA
    Theriogenology 63:2356-71. 2005
    ....
  24. ncbi request reprint Differentiation of monkey embryonic stem cells into neural lineages
    Hung Chih Kuo
    Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton 97006, USA
    Biol Reprod 68:1727-35. 2003
    ....
  25. ncbi request reprint Abnormalities in human pluripotent cells due to reprogramming mechanisms
    Hong Ma
    1 Center for Embryonic Cell and Gene Therapy, Oregon Health and Science University, 3303 Southwest Bond Avenue, Portland, Oregon 97239, USA 2 Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 Northwest 185th Avenue, Beaverton, Oregon 97006, USA 3
    Nature 511:177-83. 2014
    ..Thus, human somatic cells can be faithfully reprogrammed to pluripotency by SCNT and are therefore ideal for cell replacement therapies. ..
  26. pmc Mitochondrial replacement therapies can circumvent mtDNA-based disease transmission
    Don P Wolf
    Center for Embryonic Cell and Gene Therapy, Oregon Health and Science University, 3303 Southwest Bond Avenue, Portland, OR 97239, USA Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 Northwest 185 th Avenue, Beaverton, OR 97006, USA
    Cell Metab 20:6-8. 2014
    ..A study in Cell by Wang et al. (2014) adds the exciting possibility of a new therapy for preventing mitochondrial disease transmission predicated on the use of polar body genomes in mice. ..
  27. ncbi request reprint Oct-4 expression in pluripotent cells of the rhesus monkey
    Shoukhrat M Mitalipov
    Oregon National Primate Research Center, Beaverton, Oregon 97006, USA
    Biol Reprod 69:1785-92. 2003
    ..Finally, the observed lack or abnormal expression of Oct-4 in monkey nuclear transfer embryos suggests inadequate nuclear reprogramming...
  28. ncbi request reprint Rhesus monkey embryos produced by nuclear transfer from embryonic blastomeres or somatic cells
    Shoukhrat M Mitalipov
    Oregon Regional Primate Research Center, Beaverton, OR 97006, USA
    Biol Reprod 66:1367-73. 2002
    ..Future efforts will focus on optimizing the production of somatic cell NT embryos that develop in high efficiency to the blastocyst stage in vitro...
  29. ncbi request reprint Transcriptional profiling of rhesus monkey embryonic stem cells
    James A Byrne
    Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon 97006, USA
    Biol Reprod 75:908-15. 2006
    ..These five mammalian genes are strongly expressed in rhesus monkey, mouse, and human ESCs, albeit only in the undifferentiated state, and represent the core key mammalian stemness factors...
  30. ncbi request reprint Nuclear transfer in nonhuman primates
    Shoukhrat M Mitalipov
    Oregon National Primate Research Center, Beaverton, USA
    Methods Mol Biol 348:151-68. 2006
    ..In this chapter, we describe detailed protocols for rhesus macaque embryonic cell NT, including oocyte and embryo production, micromanipulation, and embryo transfer in nonhuman primates...
  31. ncbi request reprint Monozygotic twinning in rhesus monkeys by manipulation of in vitro-derived embryos
    Shoukhrat M Mitalipov
    Oregon Regional Primate Research Center, Oregon Health and Science University, Beaverton, OR 97006, USA
    Biol Reprod 66:1449-55. 2002
    ..Neither pregnancy resulted in term birth of monozygotic twins, but our results are sufficiently encouraging to justify a large-scale twinning trial in the rhesus macaque...
  32. ncbi request reprint Evaluation of the vervet (Clorocebus aethiops) as a model for the assisted reproductive technologies
    Michelle L Sparman
    Division of Reproductive Sciences, Oregon National Primate Research Center, Beaverton, OR 97006, USA
    Am J Primatol 69:917-29. 2007
    ..These results emphasize the need to establish species-specific ovarian stimulation protocols and semen collection techniques if vervets are to be considered for basic and applied (ARTs) research on primate gametes or embryos...
  33. ncbi request reprint Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells
    Deepika Rajesh
    Department of Surgery, University of Wisconsin Medical School, K4 760 Clinical Science Center, 600 Highland Avenue, Madison, Wisconsin 53792 7375, USA
    Stem Cells 25:490-9. 2007
    ..Although rESCs have the potential for hematopoietic differentiation, they exhibit a pause at the hemangioblast stage of hematopoietic development in culture conditions developed for hESCs...
  34. ncbi request reprint Aberrant genomic imprinting in rhesus monkey embryonic stem cells
    Akihisa Fujimoto
    Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Japan
    Stem Cells 24:595-603. 2006
    ..In differentiated ESC progeny, these expression patterns were maintained. The implications of aberrant imprinted gene expression to ESC differentiation in vitro and on ESC-derived cell function in vivo after transplantation are unknown...

Research Grants5

  1. Genetically Modified Rhesus Monkeys
    Shoukhrat Mitalipov; Fiscal Year: 2006
    ..Such animals should provide a resource for the study of human neurogenetic diseases and serve as pre-clinical models for new experimental treatments including gene and stem cell based therapies. ..
  2. Histocompatible Primate Embryonic Stem Cells
    Shoukhrat Mitalipov; Fiscal Year: 2009
    ..The outcomes of these experiments, in turn, will allow the production of useful preclinical monkey models for testing therapeutic applications involving autologous stem cells for the treatment of wide range of degenerative diseases. ..
  3. Altered Nuclear Transfer
    Shoukhrat M Mitalipov; Fiscal Year: 2010
    ..The outcomes of these experiments, in turn, will allow the production of useful preclinical monkey models for testing therapeutic applications involving autologous stem cells for the treatment of wide range of degenerative diseases. ..
  4. Mitochondrial Gene Therapy
    Shoukhrat M Mitalipov; Fiscal Year: 2010
    ..In this study, we will explore feasibility, efficiency and safety of this novel mitochondrial gene replacement therapy in a clinically relevant nonhuman primate model. ..
  5. Histocompatible Primate Embryonic Stem Cells
    Shoukhrat M Mitalipov; Fiscal Year: 2010
    ..The outcomes of these experiments, in turn, will allow the production of useful preclinical monkey models for testing therapeutic applications involving autologous stem cells for the treatment of wide range of degenerative diseases. ..