DAVID LEWINSOHN

Summary

Affiliation: Oregon Health and Science University
Country: USA

Publications

  1. pmc HLA-E-dependent presentation of Mtb-derived antigen to human CD8+ T cells
    Amy S Heinzel
    Division of Pulmonary and Critical Care Medicine, Portland VA Medical Center, Oregon Health Sciences University, Portland, OR 97201, USA
    J Exp Med 196:1473-81. 2002
  2. pmc TAP mediates import of Mycobacterium tuberculosis-derived peptides into phagosomes and facilitates loading onto HLA-I
    Melanie J Harriff
    Portland VA Medical Center, Portland, Oregon, United States of America Department of Pulmonary and Critical Care Medicine, Oregon Health and Sciences University, Portland, Oregon, United States of America
    PLoS ONE 8:e79571. 2013
  3. pmc The Mycobacterium tuberculosis phagosome is a HLA-I processing competent organelle
    Jeff E Grotzke
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR, USA
    PLoS Pathog 5:e1000374. 2009
  4. pmc Escape from the Phagosome: The Explanation for MHC-I Processing of Mycobacterial Antigens?
    Melanie J Harriff
    Portland VA Medical Center Portland, OR, USA
    Front Immunol 3:40. 2012
  5. ncbi request reprint High resolution radiographic and fine immunologic definition of TB disease progression in the rhesus macaque
    David M Lewinsohn
    Pulmonary and CCM, R and D 11, Portland VA Medical Center, 3710 SW US Veterans Road, Portland, OR 97239, USA
    Microbes Infect 8:2587-98. 2006
  6. ncbi request reprint TB vaccines at the turn of the century: insights into immunity to M. tuberculosis and modern approaches for prevention of an ancient disease
    David M Lewinsohn
    Division of Pulmonary and Critical Care Medicine, Department of Molecular Microbiology and Immunology, Oregon Health and Science University Portland VA Medical Center, Portland, OR 97201, USA
    Semin Respir Infect 18:320-38. 2003
  7. doi request reprint Views of immunology: effector T cells
    Deborah A Lewinsohn
    Pediatric Infectious Diseases, Oregon Health and Science University, Portland, OR, USA
    Immunol Rev 240:25-39. 2011
  8. ncbi request reprint Classically restricted human CD8+ T lymphocytes derived from Mycobacterium tuberculosis-infected cells: definition of antigenic specificity
    D M Lewinsohn
    Division of Pulmonary and Critical Care Medicine, Oregon Health Sciences University Portland Veterans Affairs Medical Center, 97207, USA
    J Immunol 166:439-46. 2001
  9. ncbi request reprint Human dendritic cells presenting adenovirally expressed antigen elicit Mycobacterium tuberculosis--specific CD8+ T cells
    Deborah A Lewinsohn
    Division of Pediatric Infectious Diseases, Department of Molecular Microbiology and Immunology, Oregon Health and Science University Portland VA Medical Center, Portland, OR, USA
    Am J Respir Crit Care Med 166:843-8. 2002
  10. ncbi request reprint Secreted proteins from Mycobacterium tuberculosis gain access to the cytosolic MHC class-I antigen-processing pathway
    David M Lewinsohn
    Division of Pulmonary and Critical Care Medicine, Oregon Health Sciences University Portland Veterans Affairs VA Medical Center, 3710 U S Veterans Road, Portland, OR 97239, USA
    J Immunol 177:437-42. 2006

Research Grants

  1. RHESUS MACAQUE MODEL OF TUBERCULOSIS
    DAVID LEWINSOHN; Fiscal Year: 2001

Collaborators

Detail Information

Publications38

  1. pmc HLA-E-dependent presentation of Mtb-derived antigen to human CD8+ T cells
    Amy S Heinzel
    Division of Pulmonary and Critical Care Medicine, Portland VA Medical Center, Oregon Health Sciences University, Portland, OR 97201, USA
    J Exp Med 196:1473-81. 2002
    ..This is the first demonstration of the ability of HLA-E to present pathogen-derived antigen. Further definition of the HLA-E specific response may aid development of an effective vaccine against tuberculosis...
  2. pmc TAP mediates import of Mycobacterium tuberculosis-derived peptides into phagosomes and facilitates loading onto HLA-I
    Melanie J Harriff
    Portland VA Medical Center, Portland, Oregon, United States of America Department of Pulmonary and Critical Care Medicine, Oregon Health and Sciences University, Portland, Oregon, United States of America
    PLoS ONE 8:e79571. 2013
    ..5 protein, which is capable of specifically inhibiting the lumenal face of TAP. Together, these results provide insight into the mechanism by which peptides from intra-phagosomal pathogens are loaded onto Class I molecules. ..
  3. pmc The Mycobacterium tuberculosis phagosome is a HLA-I processing competent organelle
    Jeff E Grotzke
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR, USA
    PLoS Pathog 5:e1000374. 2009
    ..This is, to our knowledge, the first description of this presentation pathway for an intracellular pathogen. Moreover, these data suggest that HLA-E may play a unique role in the presentation of phagosomal antigens...
  4. pmc Escape from the Phagosome: The Explanation for MHC-I Processing of Mycobacterial Antigens?
    Melanie J Harriff
    Portland VA Medical Center Portland, OR, USA
    Front Immunol 3:40. 2012
    ..In this commentary, we will review data on the escape of Mtb from the cytosol and whether this escape is required for antigen presentation to CD8(+) T cells...
  5. ncbi request reprint High resolution radiographic and fine immunologic definition of TB disease progression in the rhesus macaque
    David M Lewinsohn
    Pulmonary and CCM, R and D 11, Portland VA Medical Center, 3710 SW US Veterans Road, Portland, OR 97239, USA
    Microbes Infect 8:2587-98. 2006
    ..In summary, computed tomography scanning in conjunction with immunologic monitoring provides a non-invasive, accurate, and rapid assessment of tuberculosis in the non-human primate...
  6. ncbi request reprint TB vaccines at the turn of the century: insights into immunity to M. tuberculosis and modern approaches for prevention of an ancient disease
    David M Lewinsohn
    Division of Pulmonary and Critical Care Medicine, Department of Molecular Microbiology and Immunology, Oregon Health and Science University Portland VA Medical Center, Portland, OR 97201, USA
    Semin Respir Infect 18:320-38. 2003
    ....
  7. doi request reprint Views of immunology: effector T cells
    Deborah A Lewinsohn
    Pediatric Infectious Diseases, Oregon Health and Science University, Portland, OR, USA
    Immunol Rev 240:25-39. 2011
    ....
  8. ncbi request reprint Classically restricted human CD8+ T lymphocytes derived from Mycobacterium tuberculosis-infected cells: definition of antigenic specificity
    D M Lewinsohn
    Division of Pulmonary and Critical Care Medicine, Oregon Health Sciences University Portland Veterans Affairs Medical Center, 97207, USA
    J Immunol 166:439-46. 2001
    ..This study represents the first time CD8(+) T cells generated against Mtb-infected APC have been used to elucidate an Mtb-specific CD8(+) T cell Ag...
  9. ncbi request reprint Human dendritic cells presenting adenovirally expressed antigen elicit Mycobacterium tuberculosis--specific CD8+ T cells
    Deborah A Lewinsohn
    Division of Pediatric Infectious Diseases, Department of Molecular Microbiology and Immunology, Oregon Health and Science University Portland VA Medical Center, Portland, OR, USA
    Am J Respir Crit Care Med 166:843-8. 2002
    ..The technique will be useful in defining CD8+ T cell antigens and in measuring immunogenicity of tuberculosis vaccines...
  10. ncbi request reprint Secreted proteins from Mycobacterium tuberculosis gain access to the cytosolic MHC class-I antigen-processing pathway
    David M Lewinsohn
    Division of Pulmonary and Critical Care Medicine, Oregon Health Sciences University Portland Veterans Affairs VA Medical Center, 3710 U S Veterans Road, Portland, OR 97239, USA
    J Immunol 177:437-42. 2006
    ..We conclude that secreted proteins from Mtb can be processed in a BFA- and proteasome-dependent manner, consistent with egress of Ag into the cytosol and subsequent loading of proteasomally derived peptides...
  11. ncbi request reprint Mycobacterium tuberculosis-reactive CD8+ T lymphocytes: the relative contribution of classical versus nonclassical HLA restriction
    D M Lewinsohn
    Division of Pulmonary and Critical Care Medicine, Oregon Health Sciences, University Portland Veterans Affairs Medical Center, Portland, OR 97201, USA
    J Immunol 165:925-30. 2000
    ..Further definition of the nonclassical response may aid development of an effective vaccine against tuberculosis...
  12. ncbi request reprint Rudimentary TCR signaling triggers default IL-10 secretion by human Th1 cells
    G G Burrows
    Department of Neurology, Oregon Health and Science University, Portland, OR 97201, USA
    J Immunol 167:4386-95. 2001
    ..Activation with RTLs provides a novel strategy for generating autoantigen-specific bystander suppression useful for treatment of complex autoimmune diseases...
  13. ncbi request reprint T-cell clones derived by CD3 stimulation from hepatitis C virus explanted liver tissue are not representative of dominant clones present in vivo
    M P Davey
    Department of Veterans Affairs Medical Center, Oregon Health Sciences University, 3710 SW U S Veterans Hospital Rd, Bldg 101, Rm 502, Portland, OR 97201, USA
    Liver Transpl 7:716-23. 2001
    ....
  14. ncbi request reprint Tuberculosis immunology in children: diagnostic and therapeutic challenges and opportunities
    D A Lewinsohn
    Department of Pediatrics, Oregon Health and Science University, Portland 97239, USA
    Int J Tuberc Lung Dis 8:658-74. 2004
    ..tuberculosis-specific T-cell immunity in children, and potential application to immunodiagnostics and vaccine development will be reviewed...
  15. pmc Characterization of human CD8+ T cells reactive with Mycobacterium tuberculosis-infected antigen-presenting cells
    D M Lewinsohn
    Division of Pulmonary and Critical Care Medicine, University of Washington, Seattle, Washington 98104, USA
    J Exp Med 187:1633-40. 1998
    ..The data suggest the possible use of nonpolymorphic MHC class Ib antigen presenting structures other than CD1...
  16. pmc Individual RD1-region genes are required for export of ESAT-6/CFP-10 and for virulence of Mycobacterium tuberculosis
    Kristi M Guinn
    Department of Pathobiology, University of Washington, Seattle, WA 98195, USA
    Mol Microbiol 51:359-70. 2004
    ..Our results indicate that RD1 genes work together to form a single virulence determinant, and argue that RD1 encodes a novel specialized secretion system that is required for pathogenesis of MTB...
  17. pmc Inhibition of HLA-DR assembly, transport, and loading by human cytomegalovirus glycoprotein US3: a novel mechanism for evading major histocompatibility complex class II antigen presentation
    Nagendra R Hegde
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Virol 76:10929-41. 2002
    ..We concluded that US3 and US2 can collaborate to inhibit class II-mediated presentation of endogenous HCMV antigens to CD4(+) T cells, allowing virus-infected cells to resist recognition by CD4(+) T cells...
  18. pmc Immunodominant tuberculosis CD8 antigens preferentially restricted by HLA-B
    Deborah A Lewinsohn
    Department of Pediatrics, Oregon Health and Sciences University, Portland, Oregon, USA
    PLoS Pathog 3:1240-9. 2007
    ..These findings provide an improved understanding of immunodominance in humans and may contribute to a development of an effective TB vaccine and improved immunodiagnostics...
  19. pmc Human neonatal dendritic cells are competent in MHC class I antigen processing and presentation
    Marielle C Gold
    Department of Pulmonary and Critical Care Medicine, Oregon Health and Science University, Portland, Oregon, USA
    PLoS ONE 2:e957. 2007
    ....
  20. pmc Profiling antibodies to Mycobacterium tuberculosis by multiplex microbead suspension arrays for serodiagnosis of tuberculosis
    Imran H Khan
    Center for Comparative Medicine, Department of Pathology and Laboratory Medicine, School of Medicine, University of California, Davis, California 95616, USA
    Clin Vaccine Immunol 15:433-8. 2008
    ..In addition, the MMIA serodetection system has a potential for mining M. tuberculosis open reading frames (about 4,000) to discover novel target proteins for the development of more-comprehensive TB serodiagnostic tests...
  21. pmc Human innate Mycobacterium tuberculosis-reactive alphabetaTCR+ thymocytes
    Marielle C Gold
    Department of Pulmonary and Critical Care Medicine, Portland VA Medical Center, Oregon Health and Science University, Portland, Oregon, United States of America
    PLoS Pathog 4:e39. 2008
    ..Furthermore, this is the first demonstration of a human innate pathogen-specific alphabetaTCR(+) T cell and is likely to inspire further investigation into innate T cells recognizing other important human pathogens...
  22. pmc Interferon-gamma release assays for diagnosing mycobacterium tuberculosis infection in renal dialysis patients
    Kevin L Winthrop
    Division of Tuberculosis Elimination, National Center for HIV, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    Clin J Am Soc Nephrol 3:1357-63. 2008
    ..This study sought to determine whether IFN-gamma release assay results are more closely associated with recent TB exposure than TST results...
  23. ncbi request reprint Infection of APC by human cytomegalovirus controlled through recognition of endogenous nuclear immediate early protein 1 by specific CD4(+) T lymphocytes
    Emmanuelle Le Roy
    Institut National de la Sante et de la Recherche Medicale, Toulouse, France
    J Immunol 169:1293-301. 2002
    ....
  24. ncbi request reprint HIV-1 Vpr does not inhibit CTL-mediated apoptosis of HIV-1 infected cells
    Deborah A Lewinsohn
    Department of Pediatrics, Oregon Health and Sciences University, 707 SW Gaines Road, CDRCP, Portland, OR 97201, USA
    Virology 294:13-21. 2002
    ..Furthermore, we confirm that HIV-1 Nef is able to provide partial protection from CTL recognition of infected cells. Thus, the inability of CTL to control HIV-1 infection is likely not due to direct inhibition of CTL-mediated apoptosis...
  25. ncbi request reprint Frequencies of HCV-specific effector CD4+ T cells by flow cytometry: correlation with clinical disease stages
    Hugo R Rosen
    Department of Medicine, Portland Veterans Affairs Medical Center Oregon Health and Science University, Portland OR 97207, USA
    Hepatology 35:190-8. 2002
    ..Prospective studies using this new flow cytometric assay will be required to determine whether antiviral therapy modifies the frequency, specificity, and function of these virus-specific effector cells...
  26. ncbi request reprint Differential antigenic hierarchy associated with spontaneous recovery from hepatitis C virus infection: implications for vaccine design
    Susan Smyk-Pearson
    Division of Gastroenterology and Hepatology, Hepatitis C Center, and Integrated Program in Immunology, University of Colorado Health Sciences Center and National Jewish Hospital, Denver, 80262, USA
    J Infect Dis 194:454-63. 2006
    ..The development of vaccine candidates to prevent the spread of this infection remains a top priority; however, understanding the correlates of effective immunological containment is an important prerequisite...
  27. ncbi request reprint Novel CD4+ and CD8+ T-cell determinants within the NS3 protein in subjects with spontaneously resolved HCV infection
    Anne M Wertheimer
    Department of Medicine and Research Services, Portland Veterans Affairs Medical Center Oregon Health and Science University, Portland, OR 97207, USA
    Hepatology 37:577-89. 2003
    ..By providing a comprehensive screening of all potential T-cell epitopes contained in the NS3 region, our strategy defines the breadth of the T-cell response and identifies novel, unpredicted specificities...
  28. ncbi request reprint Characterization of a Mycobacterium tuberculosis peptide that is recognized by human CD4+ and CD8+ T cells in the context of multiple HLA alleles
    Homayoun Shams
    Center for Pulmonary and Infectious Disease Control, and Department of Microbiology, University of Texas Health Center, Tyler, TX 75708, USA
    J Immunol 173:1966-77. 2004
    ....
  29. ncbi request reprint Cutting edge: identification of hepatitis C virus-specific CD8+ T cells restricted by donor HLA alleles following liver transplantation
    Hugo R Rosen
    Hepatology and Liver Transplantation Program, Portland Veterans Affairs Medical Center Oregon Health and Sciences University, Portland, OR 97207, USA
    J Immunol 173:5355-9. 2004
    ..The in vitro specificity to donor HLA allele-restricted CD8+ T cells suggests that these cells may function to control HCV spread in the allograft...
  30. ncbi request reprint Identification of a human HLA-E-restricted CD8+ T cell subset in volunteers immunized with Salmonella enterica serovar Typhi strain Ty21a typhoid vaccine
    Rosângela Salerno-Goncalves
    Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Immunol 173:5852-62. 2004
    ....
  31. ncbi request reprint Mycobacterium tuberculosis-specific CD8+ T cells preferentially recognize heavily infected cells
    Deborah A Lewinsohn
    Department of Pediatrics, Oregon Health and Science University, Portland, Oregon 97239, USA
    Am J Respir Crit Care Med 168:1346-52. 2003
    ....
  32. ncbi request reprint Role of CD8+ T lymphocytes in control of Mycobacterium tuberculosis infection
    Jeff E Grotzke
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    Microbes Infect 7:776-88. 2005
    ..In this article, data supporting the importance of CD8+ T lymphocytes is reviewed, with an emphasis on the unique functional roles that this lymphocyte subset may play...
  33. pmc Immune evasion versus recovery after acute hepatitis C virus infection from a shared source
    Ian Tester
    Department of Medicine, Portland Veterans Administration Medical Center, Oregon Health Science University, Portland, OR 97239, USA
    J Exp Med 201:1725-31. 2005
    ..Statistical evidence for positive Darwinian selective pressure against an immunodominant epitope is presented. Wild-type cytotoxic T lymphocytes persisted even after the cognate antigen was no longer present...
  34. pmc Endogenous human cytomegalovirus gB is presented efficiently by MHC class II molecules to CD4+ CTL
    Nagendra R Hegde
    Department of Molecular Microbiology and Immunology, Veterans Affairs Medical Center, Portland, OR 97239, USA
    J Exp Med 202:1109-19. 2005
    ....
  35. ncbi request reprint Alterations in T-cell subset frequency in peripheral blood in obesity
    ROBERT W O'ROURKE
    Department of Surgery, Oregon Health and Science University, Portland, OR 97239, USA
    Obes Surg 15:1463-8. 2005
    ..Obesity affects the regulation of immune and inflammatory responses. This study characterizes differences in peripheral blood lymphocyte phenotype in obese humans...
  36. ncbi request reprint Reconstitution of hepatitis C virus-specific T-cellmediated immunity after liver transplantation
    Scott J Weston
    Department of Medicine, Oregon Health and Science University, USA
    Hepatology 41:72-81. 2005
    ..Supplementary material for this article can be found on the HEPATOLOGY website (http://interscience. wiley.com/jpages/0270-9139/suppmat/index.html)...
  37. doi request reprint Immunologic susceptibility of young children to Mycobacterium tuberculosis
    Deborah A Lewinsohn
    Pediatr Res 63:115. 2008
  38. ncbi request reprint Interferon-gamma assays for tuberculosis: is anergy the Achilles' heel?
    Madhukar Pai
    Am J Respir Crit Care Med 172:519-21. 2005

Research Grants1

  1. RHESUS MACAQUE MODEL OF TUBERCULOSIS
    DAVID LEWINSOHN; Fiscal Year: 2001
    ..g., CT scans, quantitative culture of mycobacteria from BAL fluids and liver biopsies) which correlate with survival. ..