Research Topics
Genomes and GenesSpecies | MARKUS C GROMPESummaryAffiliation: Oregon Health and Science University Country: USA Publications
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Publications
Tissue stem cells: new tools and functional diversityMarkus Grompe
Papé Family Pediatric Research Institute, Oregon Health and Science University, 3181 S W Sam Jackson Park Road, Mail Code L321, Portland, OR 97239 3098, USA
Cell Stem Cell 10:685-9. 2012..Furthermore, some tissues have more than one type of stem cell, each used in only particular situations of regenerative stress. Thus, there is no "one size fits all" adult tissue stem cell paradigm...- Principles of therapeutic liver repopulationM Grompe
Department of Molecular and Medical Genetics, Oregon Health Sciences University, Portland 97201, USA
Semin Liver Dis 19:7-14. 1999..Here we review the current knowledge of this process and discuss the hopeful implications for treatment of liver diseases... - The role of bone marrow stem cells in liver regenerationMarkus Grompe
Department of Molecular and Medical Genetics L103, Oregon Health and Science University, Portland, Oregon 97239, USA
Semin Liver Dis 23:363-72. 2003..Second, hematopoietic cells, including lymphocytes, neutrophils, macrophages, and platelets, may provide crucial factors required for efficient healing of damaged liver... - Adult versus embryonic stem cells: it's still a tieMarkus Grompe
Department of Molecular and Medical Genetics L103, 3181 SW Sam Jackson Park Road, Portland, Oregon 97201, USA
Mol Ther 6:303-5. 2002 - The Fanconi family adds a fraternal twinMarkus Grompe
Oregon Stem Cell Center, Oregon Health Science University, 3181 S W Sam Jackson Park Road, Mail Code L321, Portland, OR 97239, USA
Dev Cell 12:661-2. 2007..Like FANCD2, monoubiquitination of FANCI requires the FA core complex. Importantly, FANCI and FANCD2 monoubiquitination is co-dependent, suggesting a novel mechanism in ubiquitin conjugation... - Transition of stem cells to therapeutically functional tissue-specific cellsMarkus Grompe
Oregon Health Science University, 31181 SW Sam Jackson Park Road, Portland, OR 97201, USA
Ann N Y Acad Sci 961:305-6. 2002 - The origin of hepatocytesMarkus Grompe
Oregon Stem Cell Center, Oregon Health and Science University, Portland 97239, USA
Gastroenterology 128:2158-60. 2005 - Principles of therapeutic liver repopulationMarkus Grompe
Oregon Stem Cell Center, Oregon Health and Science University, Portland, Oregon, USA
J Inherit Metab Dis 29:421-5. 2006..In this review, examples of liver repopulation in animals and the implications of these models for clinical cell transplantation will be discussed... - The pathophysiology and treatment of hereditary tyrosinemia type 1M Grompe
Department of Molecular and Medical Genetics, Oregon Health Sciences University, Portland, Oregon 97201, USA
Semin Liver Dis 21:563-71. 2001.... - Fanconi anemia and DNA repairM Grompe
Department of Molecular and Medical Genetics, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road L103, Portland, OR 97201, USA
Hum Mol Genet 10:2253-9. 2001..Together, these data suggest that the FA pathway functions primarily as a DNA damage response system, although its exact role (direct involvement in DNA repair versus indirect, facilitating role) has not yet been defined... - Liver repopulation for the treatment of metabolic diseasesM Grompe
Department of Molecular and Medical Genetics, Department of Pediatrics, Oregon Health Sciences University, Portland 97201, USA
J Inherit Metab Dis 24:231-44. 2001..Here, the current knowledge regarding therapeutic liver repopulation and the hopeful implications for treatment of liver diseases will be discussed... - Therapeutic liver repopulation for the treatment of metabolic liver diseasesM Grompe
Dept of Molecular and Medical Genetics, Oregon Health Sciences University, Portland 97201, USA
Hum Cell 12:171-80. 1999..Here we will review the current knowledge of this process and discuss the hopeful implications for treatment of liver diseases... - CXCR4 induction in hematopoietic progenitor cells from Fanca(-/-), -c(-/-), and -d2(-/-) miceAmy M Skinner
Department of Pediatrics, Oregon Health and Science University, Portland, OR 97239, USA
Exp Hematol 36:273-82. 2008..However, inherent repopulation deficits and stem cell attrition during conventional transduction culture prevent therapeutic chimerism... - The origin and liver repopulating capacity of murine oval cellsXin Wang
Department of Molecular and Medical Genetics, Oregon Health Sciences University, 3181 Southwest Sam Jackson Park Road, Portland, OR 97239, USA
Proc Natl Acad Sci U S A 100:11881-8. 2003..We conclude that hepatic oval cells do not originate in bone marrow but in the liver itself, and that they have valuable properties for therapeutic liver repopulation... - In vivo genetic selection of renal proximal tubulesPatrice K Held
Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, OR 97239, USA
Mol Ther 13:49-58. 2006..These data demonstrate that extensive regeneration of the renal proximal tubule compartment can be achieved through genetic selection of functional cells... - In vivo correction of murine tyrosinemia type I by DNA-mediated transpositionEugenio Montini
Department of Medical and Molecular Genetics, Oregon Health and Sciences University, Portland, Oregon 97239, USA
Mol Ther 6:759-69. 2002..Molecular analysis indicated that high-efficiency DNA-mediated transposition into the mouse genome was strictly dependent on the expression of wild-type transposase... - Cell fusion is the principal source of bone-marrow-derived hepatocytesXin Wang
Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, Oregon 97239, USA
Nature 422:897-901. 2003..We conclude that hepatocytes derived form bone marrow arise from cell fusion and not by differentiation of haematopoietic stem cells... - Kinetics of liver repopulation after bone marrow transplantationXin Wang
Department of Molecular and Medical Genetics, Oregon Health and Sciences University, Portland 97201, USA
Am J Pathol 161:565-74. 2002..We conclude that hepatocyte replacement by bone marrow cells is a slow and rare event. Significant improvements in the efficiency of this process will be needed before clinical success can be expected... - Stem cells and liver regenerationAndrew W Duncan
Oregon Stem Cell Center, Oregon Health and Science University, Portland, Oregon 97239 3098, USA
Gastroenterology 137:466-81. 2009..This review will consider liver stem cells in the context of each definition... - Embryonic lethality after combined inactivation of Fancd2 and Mlh1 in miceHenri J van de Vrugt
Oregon Stem Cell Center, Oregon Health and Science University, Portland, Oregon, USA
Cancer Res 69:9431-8. 2009.... - Robust expansion of human hepatocytes in Fah-/-/Rag2-/-/Il2rg-/- miceHisaya Azuma
Oregon Stem Cell Center, Oregon Health and Science University, Portland, Oregon 97239, USA
Nat Biotechnol 25:903-10. 2007..It may also be useful for testing the toxicity of drug metabolites and for evaluating pathogens dependent on human liver cells for replication... - Liver-directed adenoviral gene transfer in murine succinate semialdehyde dehydrogenase deficiencyManeesh Gupta
Molecular and Medical Genetics, Oregon Health and Sciences University, Portland, OR 97239, USA
Mol Ther 9:527-39. 2004..Our studies provide proof-of-principle that liver-mediated gene therapy has efficacy in treating SSADH deficiency and that hepatic tissue contributes significantly to the pool of GHB within the CNS... - Sustained phosphorylation of Bid is a marker for resistance to Fas-induced apoptosis during chronic liver diseasesArndt Vogel
Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, Oregon, USA
Gastroenterology 130:104-19. 2006..To clarify whether stress-induced apoptosis resistance is a general feature of chronic liver diseases, an animal model of chronic cholestasis was examined... - Therapeutic liver reconstitution with murine cells isolated long after deathLaura Erker
Oregon Stem Cell Center, Oregon Health and Science University, Portland, Oregon 97239, USA
Gastroenterology 139:1019-29. 2010..For some liver diseases, hepatocyte transplantation could be a viable alternative, but donor cells currently are procured from the same sources as whole organs, and thus the supply is severely limited... - Liver repair by intra- and extrahepatic progenitorsCraig Dorrell
Department of Molecular and Medical Genetics, Oregon Stem Cell Center, Oregon Health and Science University, Portland, OR 97239, USA
Stem Cell Rev 1:61-4. 2005..As is the case for the delivery of intrahepatic progenitors, substantial improvements in the understanding of this process will be needed before clinical application becomes practical... - Fancd2-/- mice have hematopoietic defects that can be partially corrected by resveratrolQing Shuo Zhang
Oregon Stem Cell Center, Department of Pediatrics, Oregon Health and Science University, Portland, OR, USA
Blood 116:5140-8. 2010..We conclude that Fancd2(-/-) mice have readily quantifiable hematopoietic defects, and that this model is well suited for pharmacologic screening studies... - The ploidy conveyor of mature hepatocytes as a source of genetic variationAndrew W Duncan
Oregon Stem Cell Center, Papé Family Pediatric Research Institute, Oregon Health and Science University, Portland, Oregon 97239, USA
Nature 467:707-10. 2010..We propose that this mechanism evolved to generate genetic diversity and permits adaptation of hepatocytes to xenobiotic or nutritional injury... - Bone marrow-derived cells fuse with normal and transformed intestinal stem cellsAdnan Z Rizvi
Department of Surgery, Division of Hematology and Medical Oncology, and Oregon Cancer Institute, Oregon Stem Cell Center, Oregon Health and Science University, 3181 Southwest Sam Jackson Park Road, Portland, OR 97239, USA
Proc Natl Acad Sci U S A 103:6321-5. 2006..Fusion of BMDCs with neoplastic epithelium did not result in tumor initiation. Our findings suggest an unexpected role for BMDCs in both regeneration and tumorigenesis of the intestine... - ERCC1 is required for FANCD2 focus formationKevin M McCabe
Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, OR 97239, USA
Mol Genet Metab 95:66-73. 2008..Our results support a role for ERCC1 after creation of a double strand break for full activation of the Fanconi anemia pathway... - Tempol protects against oxidative damage and delays epithelial tumor onset in Fanconi anemia miceQing Shuo Zhang
Oregon Stem Cell Center, Oregon Health and Science University, Portland, OR 97239, USA
Cancer Res 68:1601-8. 2008..The reduction in oxidative DNA damage in tempol-treated FA fibroblasts and mice suggests that its tumor-delaying function may be attributed to its antioxidant activity... - Non-invasive stem cell therapy in a rat model for retinal degeneration and vascular pathologyShaomei Wang
Casey Eye Institute, Oregon Health and Science University, Portland, Oregon, United States of America
PLoS ONE 5:e9200. 2010.... - Adeno-associated virus gene repair corrects a mouse model of hereditary tyrosinemia in vivoNICOLE K PAULK
Oregon Stem Cell Center, Oregon Health and Science University, Portland, OR 97203, USA
Hepatology 51:1200-8. 2010..Conclusion: AAV-mediated gene repair is feasible in vivo and can functionally correct an appropriate selection-based metabolic liver disease in both adults and neonates... - Surface markers for the murine oval cell responseCraig Dorrell
Oregon Health and Science University and the Oregon Stem Cell Center, Portland, OR 97239, USA
Hepatology 48:1282-91. 2008..CONCLUSION: A new panel of surface reactive monoclonal antibodies to support investigation of the murine oval cell response has been developed... - Ploidy reductions in murine fusion-derived hepatocytesAndrew W Duncan
Oregon Stem Cell Center, Oregon Health and Science University, Portland, Oregon, United States of America
PLoS Genet 5:e1000385. 2009..The generation of such daughter cells increases liver diversity, which may increase the likelihood of oncogenesis... - Signaling networks in hepatic oval cell activationLaura Erker
Oregon Stem Cell Center, Oregon Health Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
Stem Cell Res 1:90-102. 2007..This review describes what is currently known about the factors involved in oval cell activation, proliferation, migration, and differentiation... - Isolation of major pancreatic cell types and long-term culture-initiating cells using novel human surface markersCraig Dorrell
Oregon Health and Science University, Oregon Stem Cell Center, Portland, OR 97239, USA
Stem Cell Res 1:183-94. 2008..These methods permit the analysis and isolation of functionally distinct pancreatic cell populations with potential for cell therapy... - Fancd2 functions in a double strand break repair pathway that is distinct from non-homologous end joiningScott Houghtaling
Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, 97239, USA
Hum Mol Genet 14:3027-33. 2005..The role of Fancd2 in DSB repair may account for the moderate sensitivity of FA cells to irradiation and FA cells sensitivity to ICLs that are repaired via a DSB intermediate... - Myelomonocytic cells are sufficient for therapeutic cell fusion in liverHolger Willenbring
Department of Molecular and Medical Genetics, Oregon Stem Cell Center, Oregon Health and Science University, Portland, Oregon 97239, USA
Nat Med 10:744-8. 2004..Because stable bone marrow engraftment or HSCs are not required for this process, macrophages or their highly proliferative progenitors provide potential for targeted and well-tolerated cell therapy aimed at organ regeneration... - Interstrand crosslink-induced radials form between non-homologous chromosomes, but are absent in sex chromosomesAmy E Hanlon Newell
Department of Molecular and Medical Genetics, Oregon Health and Science University, MP350, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
DNA Repair (Amst) 3:535-42. 2004..Both X and Y chromosomes were notably not involved in radials. These observations suggest that ICL repair may involve short stretches of homology, resulting in aberrant radial formation in the absence of FA proteins... - Repair kinetics of genomic interstrand DNA cross-links: evidence for DNA double-strand break-dependent activation of the Fanconi anemia/BRCA pathwayAndreas Rothfuss
Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, Oregon 97239, USA
Mol Cell Biol 24:123-34. 2004..We therefore conclude that the FA/BRCA pathway, while being dispensable for the early events in ICL repair, is activated in S-phase cells after DSB have formed... - Epithelial cancer in Fanconi anemia complementation group D2 (Fancd2) knockout miceScott Houghtaling
Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, Oregon 97239, USA
Genes Dev 17:2021-35. 2003..The phenotypic overlap between Fancd2-null and Brca2/Fancd1 hypomorphic mice is consistent with a common function for both proteins in the same pathway, regulating genomic stability... - Pancreatic-hepatic switches in vivoMarkus Grompe
Department of Molecular and Medical Genetics L103, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97201, USA
Mech Dev 120:99-106. 2003..These cells may represent a stem cell reservoir with potential in cell therapy applications in the future... - BRCA1 interacts directly with the Fanconi anemia protein FANCAAlexandra Folias
Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, Oregon, USA
Hum Mol Genet 11:2591-7. 2002..The interaction does not depend on DNA damage, thus FANCA and BRCA1 are constitutively interacting. The demonstrated interaction directly connects BRCA1 to the FA pathway of DNA repair... - Fanconi anemia group A and C double-mutant mice: functional evidence for a multi-protein Fanconi anemia complexMeenakshi Noll
Department of Molecular and Medical Genetics, Oregon Health Sciences University, Portland, OR 97201, USA
Exp Hematol 30:679-88. 2002..The aim of this study was 1) to determine whether the FA group A and group C genes have identical or partially distinct functions, and 2) to have a better model for human FA... - Maleylacetoacetate isomerase (MAAI/GSTZ)-deficient mice reveal a glutathione-dependent nonenzymatic bypass in tyrosine catabolismJosé Manuel Fernández-Cañón
Department of Molecular and Medical Genetics, Oregon Health Sciences University, Portland, Oregon, USA
Mol Cell Biol 22:4943-51. 2002..A glutathione-mediated isomerization of MAA to FAA independent of MAAI enzyme was demonstrated in vitro. This nonenzymatic bypass is likely responsible for the lack of a phenotype in nonstressed MAAI mutant mice... - Delineating the hepatocyte's hematopoietic fusion partnerHolger Willenbring
Oregon Stem Cell Center and Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, Oregon, USA
Cell Cycle 3:1489-91. 2004..Now it should be feasible to increase the efficiency and assess the potential of cell fusion for the correction of a broad range of somatic cell types that can be targeted by fusion... - Heterozygosity for p53 (Trp53+/-) accelerates epithelial tumor formation in fanconi anemia complementation group D2 (Fancd2) knockout miceScott Houghtaling
Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, OR 97239, USA
Cancer Res 65:85-91. 2005..Therefore, Trp53 is required for the S phase checkpoint activation observed in Fancd2 mutant cells. Fancd2(-/-)/Trp53(-/-) cells showed an increase in aneuploidy and had multiple gross chromosomal rearrangements... - In vivo correction of murine hereditary tyrosinemia type I by phiC31 integrase-mediated gene deliveryPatrice K Held
Molecular and Medical Genetics, Oregon Health and Science University, Portland, OR 97239, USA
Mol Ther 11:399-408. 2005..The stability of transgene expression, relatively high integration frequency, and significant site specificity that characterize the phiC31 integration system suggest that it may have utility in many gene therapy settings... - Chronic liver disease in murine hereditary tyrosinemia type 1 induces resistance to cell deathArndt Vogel
Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, OR 97239, USA
Hepatology 39:433-43. 2004..Stress-induced failure of cell death programs may lead to an accumulation of damaged cells and therefore enhance the risk for cancer as observed in HT1 and other chronic liver diseases... - Bone marrow-derived hepatocytesMarkus Grompe
Department of Molecular and Medical Genetics L103, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
Novartis Found Symp 265:20-7; discussion 28-34, 92-7. 2005..Fully functional bone marrow-derived hepatocytes indeed exist, but are extremely rare and are generated by cell fusion, not stem cell differentiation... - Slow-onset inhibition of fumarylacetoacetate hydrolase by phosphinate mimics of the tetrahedral intermediate: kinetics, crystal structure and pharmacokineticsRaynard L Bateman
Arthur F Scott Laboratory of Chemistry, Reed College, 3203 SE Woodstock Blvd, Portland, OR 97202, USA
Biochem J 402:251-60. 2007..These potent inhibitors provide a means to chemically phenocopy the metabolic defects of either HT1 or FAH knockout mice and promise future pharmacological utility for hepatocyte transplantation... - Therapeutic intervention in mice deficient for succinate semialdehyde dehydrogenase (gamma-hydroxybutyric aciduria)Maneesh Gupta
Department of Molecular and Medical Genetics and Pediatrics, Oregon Health and Science University, 2525 SW 3rd Avenue, MP 350, Portland, OR 97201, USA
J Pharmacol Exp Ther 302:180-7. 2002..We conclude that taurine and NCS-382 may have therapeutic relevance in human SSADH deficiency and that the poor clinical efficacy of VGB in this disease may relate to an inability to decrease brain GHB concentrations... - Low therapeutic threshold for hepatocyte replacement in murine phenylketonuriaKelly Hamman
Department of Pediatrics, Oregon Health and Science University, Portland, OR 97239, USA
Mol Ther 12:337-44. 2005..These data suggest that restoration of phenylalanine homeostasis requires PAH activity in only a minority of hepatocytes... - Myeloid lineage progenitors give rise to vascular endotheliumAlexis S Bailey
Oregon Stem Cell Center, Center for Hematologic Malignancies, Division of Hematology and Medical Oncology, Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, 97239, USA
Proc Natl Acad Sci U S A 103:13156-61. 2006..Our findings indicate that endothelial cells are an intrinsic component of myeloid lineage differentiation and underscore the close functional relationship between the hematopoietic and vascular systems... - Helper-independent and AAV-ITR-independent chromosomal integration of double-stranded linear DNA vectors in miceHiroyuki Nakai
Department of Pediatrics, Stanford University School of Medicine, Stanford, California 94305, USA
Mol Ther 7:101-11. 2003..In addition, they may provide a clue for developing new nonviral integrating gene delivery vector systems... - AAV serotype 2 vectors preferentially integrate into active genes in miceHiroyuki Nakai
Department of Pediatrics, Stanford University School of Medicine, 300 Pasteur Dr. Rm G305A, Stanford, California 94305, USA
Nat Genet 34:297-302. 2003.... - Mutational spectrum of the succinate semialdehyde dehydrogenase (ALDH5A1) gene and functional analysis of 27 novel disease-causing mutations in patients with SSADH deficiencyShinjiro Akaboshi
Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland 97201, USA
Hum Mutat 22:442-50. 2003.... - CDX2 in the formation of the trophectoderm lineage in primate embryosHathaitip Sritanaudomchai
Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR, USA
Dev Biol 335:179-87. 2009..These results provide evidence that CDX2 plays an essential role in functional TE formation during primate embryonic development... - Complete hepatic regeneration after somatic deletion of an albumin-plasminogen activator transgene. 1991Markus Grompe
Department of Molecular and Medical Genetics, L103, 3181 SW Sam Jackson Park Road, Portland, OR 97201, USA
J Hepatol 37:422-4. 2002 - Large-scale molecular characterization of adeno-associated virus vector integration in mouse liverHiroyuki Nakai
Department of Pediatrics, 300 Pasteur Dr, Grant Bldg, Rm S374, Stanford University School of Medicine, Stanford, CA 94305, USA
J Virol 79:3606-14. 2005..Thus, the present study provides new insights into the risk of rAAV-mediated insertional mutagenesis and the mechanisms of rAAV integration... - Embryonic versus adult stem cell pluripotency: in liver only fusion mattersHolger Willenbring
Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, Oregon 97239, USA
J Assist Reprod Genet 20:393-4. 2003 - Natural gene therapy in monozygotic twins with Fanconi anemiaAnuj Mankad
Department of Molecular and Medical Genetics, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, USA
Blood 107:3084-90. 2006..This finding suggests that treating FA patients with gene therapy might require transduction of only a few hematopoietic stem cells... - Validation of Fanconi anemia complementation Group A assignment using molecular analysisNabil N Moghrabi
Department of Molecular and Medical Genetics, Clinical Genetics Laboratories, Oregon Health and Science University, 3181 S W Sam Jackson Park Road, Portland, OR 97239, USA
Genet Med 11:183-92. 2009....
Research Grants
- TRANSPLANTATION ASSAY FOR LIVER REPOPULATING CELLSMarkus Grompe; Fiscal Year: 2005..In addition, we will explore the properties of liver repopulation by transplanted hematopoietic stem cells (HSCs). ..
- Pathophysiology of Distal Tyrosine Pathway DisordersMarkus Grompe; Fiscal Year: 2007..In aim 2, we will use small molecule inhibitors of Fah to select for genetically modified hepatocytes in vivo. In aim 3, the pathophysiology of hepatic cancer in HT 1 and strategies for its chemoprevention will be studied. ..
- Cell Therapy by In Vivo FusionMarkus Grompe; Fiscal Year: 2007..Aim 3 is geared toward enhancing the efficiency of in vivo fusion and thereby increasing the number of bone marrow derived hepatocytes. Fusogenic viral envelope proteins will be used to artificially induce cell fusion. ..
- Novel Reagents For Beta Cell BiologyMarkus Grompe; Fiscal Year: 2007..Definition of both families of targets is required for a complete understanding of beta cell biology and pathophysiology ..
- TRANSPLANTATION ASSAY FOR LIVER REPOPULATING CELLSMarkus Grompe; Fiscal Year: 2009..In addition, the creation of a widely usable murine model for repopulation with human hepatocytes will facilitate translational studies in all areas of liver biology. ..
- Treatment of Distal Tyrosine Pathway DisordersMarkus Grompe; Fiscal Year: 2010..In addition, a broadly applicable platform for pharmacological growth selection of genetically modified hepatocytes is being developed. ..
- TRANSPLANTATION ASSAY FOR LIVER REPOPULATING CELLSMarkus Grompe; Fiscal Year: 2010..In addition, the creation of a widely usable murine model for repopulation with human hepatocytes will facilitate translational studies in all areas of liver biology. ..
- FASEB Conference: Mechanisms of Liver Growth and DiseaseMarkus Grompe; Fiscal Year: 2004..abstract_text> ..
- Isolation of Murine Pancreatic Liver Stem CellsMarkus Grompe; Fiscal Year: 2004..Monoclonal antibodies useful for FACS sorting of pancreatic cells will be generated. We will apply cell-sorting methods to enrich pancreatic liver stem cells. ..
- CREATION & ANALYSIS OF A MOUSE MODEL OF TYROSINEMIAMarkus Grompe; Fiscal Year: 2000..Accumulation of the hepatotoxic substrates of FAH will be induced by administration of substrate precursor or specific FAH inhibitors and in vivo selection will be monitored. ..
- TRANSPLANTATION ASSAY FOR LIVER REPOPULATING CELLSMarkus Grompe; Fiscal Year: 2000..By crossbreeding to SCID mice, we will also create an immune deficient FAH mutant mouse. These animals will be used to develop an analogous liver repopulation assay for xenogeneic donor hepatocytes from rat, primates and humans. ..
- PATHOPHYSIOLOGY AND TREATMENT OF DISORDERS IN THE DISTALMarkus Grompe; Fiscal Year: 2004....
- TRANSPLANTATION ASSAY FOR LIVER REPOPULATING CELLSMarkus Grompe; Fiscal Year: 2009..In addition, the creation of a widely usable murine model for repopulation with human hepatocytes will facilitate translational studies in all areas of liver biology. ..