PETER GORDON GILLESPIE

Summary

Affiliation: Oregon Health and Science University
Country: USA

Publications

  1. ncbi request reprint Engineering of the myosin-ibeta nucleotide-binding pocket to create selective sensitivity to N(6)-modified ADP analogs
    P G Gillespie
    Department of Physiology, The Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Biol Chem 274:31373-81. 1999
  2. ncbi request reprint Have we found the tip link, transduction channel, and gating spring of the hair cell?
    Peter G Gillespie
    Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    Curr Opin Neurobiol 15:389-96. 2005
  3. pmc Myosin I and adaptation of mechanical transduction by the inner ear
    Peter G Gillespie
    Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    Philos Trans R Soc Lond B Biol Sci 359:1945-51. 2004
  4. ncbi request reprint Myosin-1c, the hair cell's adaptation motor
    Peter G Gillespie
    Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, Portland, Oregon 97239, USA
    Annu Rev Physiol 66:521-45. 2004
  5. pmc Calmodulin binding to recombinant myosin-1c and myosin-1c IQ peptides
    Peter G Gillespie
    Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, Portland OR 97239, USA
    BMC Biochem 3:31. 2002
  6. pmc Myosin-I nomenclature
    P G Gillespie
    Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, Portland, OR 97201, USA
    J Cell Biol 155:703-4. 2001
  7. ncbi request reprint Molecular basis of mechanosensory transduction
    P G Gillespie
    Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, Portland, Oregon 97201, USA
    Nature 413:194-202. 2001
  8. ncbi request reprint Myosin-1c interacts with hair-cell receptors through its calmodulin-binding IQ domains
    Janet L Cyr
    Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, Portland, Oregon 97201, USA
    J Neurosci 22:2487-95. 2002
  9. pmc A mechanism for active hearing
    Tianying Ren
    Oregon Hearing Research Center, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, NRC 04, Portland, OR 97239 3098, USA
    Curr Opin Neurobiol 17:498-503. 2007
  10. ncbi request reprint Hair-cell mechanotransduction and cochlear amplification
    Meredith LeMasurier
    Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, Oregon 97239, USA
    Neuron 48:403-15. 2005

Research Grants

  1. Quantifying Hair Bundle Components
    Peter Gillespie; Fiscal Year: 2007
  2. Hair-Bundle Proteins
    Peter Gillespie; Fiscal Year: 2007
  3. Hair-Bundle Proteins
    Peter Gillespie; Fiscal Year: 2009
  4. Hair-Bundle Lipids
    Peter Gillespie; Fiscal Year: 2009
  5. Hair-Bundle Lipids
    Peter Gillespie; Fiscal Year: 2009
  6. Hair-Bundle Proteins
    Peter Gillespie; Fiscal Year: 2009
  7. Hair-Bundle Proteins
    PETER GORDON GILLESPIE; Fiscal Year: 2010
  8. Hair-Bundle Proteins
    Peter Gillespie; Fiscal Year: 2006
  9. HAIR BUNDLE MYOSIN ISOZYMES
    Peter Gillespie; Fiscal Year: 2001
  10. HAIR-BUNDLE PROTEOMICS
    Peter Gillespie; Fiscal Year: 2004

Collaborators

Detail Information

Publications21

  1. ncbi request reprint Engineering of the myosin-ibeta nucleotide-binding pocket to create selective sensitivity to N(6)-modified ADP analogs
    P G Gillespie
    Department of Physiology, The Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Biol Chem 274:31373-81. 1999
    ..Introduction of Y61G myosin-Ibeta molecules into a cell should permit selective inhibition by N(6)-modified ADP analogs of cellular processes dependent on myosin-Ibeta...
  2. ncbi request reprint Have we found the tip link, transduction channel, and gating spring of the hair cell?
    Peter G Gillespie
    Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    Curr Opin Neurobiol 15:389-96. 2005
    ..Although these are excellent candidates for the three components, definitive experiments supporting each identification have yet to be performed...
  3. pmc Myosin I and adaptation of mechanical transduction by the inner ear
    Peter G Gillespie
    Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    Philos Trans R Soc Lond B Biol Sci 359:1945-51. 2004
    ..The sliding-filament hypothesis for muscle contraction has thus led to a detailed understanding of the behaviour of hair cells...
  4. ncbi request reprint Myosin-1c, the hair cell's adaptation motor
    Peter G Gillespie
    Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, Portland, Oregon 97239, USA
    Annu Rev Physiol 66:521-45. 2004
    ..This review focuses on the detailed operation of the adaptation motor and the functional consequences of the incorporation of this specific myosin isozyme into the motor complex...
  5. pmc Calmodulin binding to recombinant myosin-1c and myosin-1c IQ peptides
    Peter G Gillespie
    Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, Portland OR 97239, USA
    BMC Biochem 3:31. 2002
    ..How calmodulin binds to these IQ domains is the subject of this report...
  6. pmc Myosin-I nomenclature
    P G Gillespie
    Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, Portland, OR 97201, USA
    J Cell Biol 155:703-4. 2001
    ..Despite their faults, we believe that the names adopted by the HUGO nomenclature group for genome annotation are the best compromise, and we recommend universal adoption...
  7. ncbi request reprint Molecular basis of mechanosensory transduction
    P G Gillespie
    Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, Portland, Oregon 97201, USA
    Nature 413:194-202. 2001
    ..But recent progress in both invertebrates and vertebrates is beginning to reveal the identities of proteins essential for transduction...
  8. ncbi request reprint Myosin-1c interacts with hair-cell receptors through its calmodulin-binding IQ domains
    Janet L Cyr
    Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, Portland, Oregon 97201, USA
    J Neurosci 22:2487-95. 2002
    ..The calcium-sensitive binding of calmodulin to myosin-1c may therefore modulate the interaction of the adaptation motor with other components of the transduction apparatus...
  9. pmc A mechanism for active hearing
    Tianying Ren
    Oregon Hearing Research Center, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, NRC 04, Portland, OR 97239 3098, USA
    Curr Opin Neurobiol 17:498-503. 2007
    ..We instead propose a 'cochlear transformer' mechanism to interpret cochlear performance...
  10. ncbi request reprint Hair-cell mechanotransduction and cochlear amplification
    Meredith LeMasurier
    Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, Oregon 97239, USA
    Neuron 48:403-15. 2005
    ..quot;Fast adaptation," the rapid closure of transduction channels following a mechanical stimulus, appears to be intimately involved in bundle-based amplification...
  11. pmc Myosin-I isozymes in neonatal rodent auditory and vestibular epithelia
    Rachel A Dumont
    Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, Portland, OR 97201, USA
    J Assoc Res Otolaryngol 3:375-89. 2002
    ..Myo1c therefore remains the myosin isozyme best localized to carry out transducer adaptation...
  12. ncbi request reprint Hair cells require phosphatidylinositol 4,5-bisphosphate for mechanical transduction and adaptation
    Moritoshi Hirono
    Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    Neuron 44:309-20. 2004
    ..PIP(2) depletion also causes a loss in transduction current. PIP(2) therefore plays an essential role in hair cell adaptation and transduction...
  13. pmc Hair bundles are specialized for ATP delivery via creatine kinase
    Jung Bum Shin
    Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA
    Neuron 53:371-86. 2007
    ..Consistent with this critical role in hair bundle function, the creatine kinase circuit is essential for high-sensitivity hearing as demonstrated by hearing loss in creatine kinase knockout mice...
  14. ncbi request reprint The hair cell's transduction channel
    Meredith Strassmaier
    Oregon Hearing Research Center, L335A, Oregon Health and Science University, 3181 Sam Jackson Park Road, Portland, Oregon 97201, USA
    Curr Opin Neurobiol 12:380-6. 2002
    ..At present, we are forced to speculate as to the transduction channel's identity; functional characteristics suggest, however, that it may well belong to transient receptor potential superfamily of ion channels...
  15. pmc Xenopus TRPN1 (NOMPC) localizes to microtubule-based cilia in epithelial cells, including inner-ear hair cells
    Jung Bum Shin
    Oregon Hearing Research Center and Vollum Institute, Portland, OR 97239, USA
    Proc Natl Acad Sci U S A 102:12572-7. 2005
    ..This result suggests that although TRPN1 is unlikely to be the transduction channel of stereocilia, it plays an essential role, functionally related to transduction, in the kinocilium...
  16. pmc Splice-site A choice targets plasma-membrane Ca2+-ATPase isoform 2 to hair bundles
    Jennifer K Hill
    Oregon Hearing Research Center, Vollum Institute, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Neurosci 26:6172-80. 2006
    ....
  17. ncbi request reprint Vestibular hair bundles control pH with (Na+, K+)/H+ exchangers NHE6 and NHE9
    Jennifer K Hill
    Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Neurosci 26:9944-55. 2006
    ..This mechanism allows the hair cell to remove H+ generated by Ca2+ pumping without ATP hydrolysis in the cell...
  18. ncbi request reprint Apical phosphatidylserine externalization in auditory hair cells
    Xiaorui Shi
    Oregon Hearing Research Center NRC04, Department of Otolaryngology, Head and Neck Surgery, Oregon Health and Science University, Portland, Oregon 97239, USA
    Mol Membr Biol 24:16-27. 2007
    ..These results indicate that PS exposure on the external leaflet, presumably requiring vesicular transport, results from elevation of intracellular cAMP, which can be triggered by Na(+) entry into hair cells...
  19. ncbi request reprint Seeing touch: moving closer to the worm mechanotransduction complex
    Jennifer K Hill
    Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    Curr Biol 13:R967-9. 2003
  20. pmc Mechanotransduction by hair cells: models, molecules, and mechanisms
    Peter G Gillespie
    Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA
    Cell 139:33-44. 2009
    ....
  21. pmc The R109H variant of fascin-2, a developmentally regulated actin crosslinker in hair-cell stereocilia, underlies early-onset hearing loss of DBA/2J mice
    Jung Bum Shin
    Oregon Hearing Research Center, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Neurosci 30:9683-94. 2010
    ..Because ahl8 accelerates hearing loss only in the presence of mutant cadherin 23, a component of hair-cell tip links, mechanotransduction and actin crosslinking must be functionally interrelated...

Research Grants31

  1. Quantifying Hair Bundle Components
    Peter Gillespie; Fiscal Year: 2007
    ..This project is an essential first step in understanding what makes up a hair bundle. ..
  2. Hair-Bundle Proteins
    Peter Gillespie; Fiscal Year: 2007
    ..The complex of proteins in hair cells that transduce sound are still largely unknown; the goal of this project is to identify and characterize them, as they are essential for hearing. ..
  3. Hair-Bundle Proteins
    Peter Gillespie; Fiscal Year: 2009
    ..The complex of proteins in hair cells that transduce sound are still largely unknown; the goal of this project is to identify and characterize them, as they are essential for hearing. ..
  4. Hair-Bundle Lipids
    Peter Gillespie; Fiscal Year: 2009
    ..Together, the experiments in this proposal will allow us to determine how lipids regulate transduction and adaptation. ..
  5. Hair-Bundle Lipids
    Peter Gillespie; Fiscal Year: 2009
    ..Together, the experiments in this proposal will allow us to determine how lipids regulate transduction and adaptation. ..
  6. Hair-Bundle Proteins
    Peter Gillespie; Fiscal Year: 2009
    ..The complex of proteins in hair cells that transduce sound are still largely unknown; the goal of this project is to identify and characterize them, as they are essential for hearing. ..
  7. Hair-Bundle Proteins
    PETER GORDON GILLESPIE; Fiscal Year: 2010
    ..The complex of proteins in hair cells that transduce sound are still largely unknown;the goal of this project is to identify and characterize them, as they are essential for hearing. ..
  8. Hair-Bundle Proteins
    Peter Gillespie; Fiscal Year: 2006
    ..4) Finally, a combination of approaches will be applied to further characterize and eventually identify the elusive transduction channel. ..
  9. HAIR BUNDLE MYOSIN ISOZYMES
    Peter Gillespie; Fiscal Year: 2001
    ..We expect that these dynamics experiments with FRAP will tie together properties of myosin isozymes and their binding proteins, both at steady-state and during tip-link regeneration. ..
  10. HAIR-BUNDLE PROTEOMICS
    Peter Gillespie; Fiscal Year: 2004
    ..These experiments will allow us to identify the major proteins of the bundle and allow us to focus on proteins functionally important for bundle function. ..
  11. ROLE OF CALCIUM PUMPING IN HAIR-BUNDLE FUNCTION
    Peter Gillespie; Fiscal Year: 2004
    ..If re-expression of PMCA2 in hair cells rescues auditory function in these otherwise PMCA2-deficient mice, the key location of PMCA2 in the ear must be the hair cell. ..
  12. Hair Bundle Structure and Dynamics
    PETER GORDON GILLESPIE; Fiscal Year: 2010
    ..More significantly, these experiments will allow us to design rational approaches to detecting and ameliorating hearing loss and disrupted balance. ..